Secondary walls in hyaline cells of Sphagnum

Abstract

The cytoskeleton and ultrastructural events associated with cell differentiation and secondary cell wall and pore formation in hyaline cells of Sphagnum are investigated. Microtubules reorient from random arrays in undifferentiated hyaline cells to transverse arrays in elongating cells. Once cells are fully elongated, broad bands of microtubules aggregate into a spiral that predicts the site of secondary cell wall deposition. The secondary wall has a similar fibrillar composition to the primary wall. After the secondary wall is deposited, the thin primary wall covering the pore breaks down, usually by cell-wall degradation at the centre of the pore and around its margin. Finally, the hyaline cell undergoes cytoplasmic degeneration. The orientation of microtubules associated with hyaline-cell formation and secondary cell wall patterning resembles ultrastructural development in tracheary elements of higher plants. The similarities in cytoskeletal arrays during cell differentiation and secondary-wall formation suggest a fundamental pathway of development shared by bryophytes and higher plants.