Lack of Correlation between Gpd Expression and X Chromosome Late Replication in Cultured Fibroblasts of the Kangaroo Macropus robustus

Abstract

Cultured fibroblasts and lymphocytes from M. robustus females heterozygous for the X-linked Gpd gene were examined electrophoretically and cytologically. Gpd expression in lymphocytes was restricted to the maternal allele while in fibroblasts there was also partial expression of the paternal allele. The Gpd gene is thought to be located on the long arm of the X chromosome. However, in fibroblasts the long arm of the paternal X chromosome showed no indication of an early replicating segment.