Studies on Marsupial Proteins III. N-Bromosuccinimide Cleavage of the (?-Chain of Kangaroo Haemoglobin and the Amino Acid Sequence of the N-Terminal Fragment

Abstract

The tryptophan contents of the (X- and ,a-chains of haemoglobin from the grey kangaroo Macropus giganteUB were determined by N-bromosuccinimide titration to be 1 and 2 residues respectively. Following cleavage of the tryptophyl bond in the (X-chain with N-bromosuccinimide in 8M urea solutions at pH 3·5 the N-termina.l fragment was purified by gel filtration and paper ionophoresis.