Deep Freezing of Ram Semen: Recovery of Spermatozoa After Pelleting and Comparison With Other Methods of Freezing

Abstract

Four factorial experiments were conducted in which were examined and compared factors which affect revival of ram spermatozoa after pellet.freezing and other methods of freezing. Revival of spermatozoa after pellet.freezing was the best in egg yolk-lactose (333 mM), followed by egg yolk-raffinose (333 mM), egg yolk-glucose (355 mM), and egg yolk-fructose (355 mM) diluents. Within the glycerol range of 0-7%, lactose gave the best results with 3%, raffinose and glucose with 5%, and fructose with 7% (vJv) glycerol.