The Effects of Incubation Temperature and Cold Shock on the Metabolism of Ram Spermatozoa

Abstract

The glycolysis of ram semen was measured at 37, 21, and 5°C. Measurements of QI0 over this range gave values between about 2 and 3 for changes in lactic acid and total reducing substances. Cold shock greatly reduced glycolysis and dehydrogenase activity which were only partially maintained by egg yolk and lecithin.