Enzymic Activities of Sub-Cellular Particles from Leaves III. Centrifugal Fractionation and Characterization of Particles in Homogenates of Green Leaves

Abstract

Homogellatos of greon poa leaves have been fractionated difforential oentrifugation. fractions so obtained examined mieroscopioally and assayed for total nitrogen, deoxyribo-, and ribonuclcic acid, stareh. chlorophyll contonj",s, sueeinic dehydrogenase, slwcinoxidase. (t-ketoglntarate cytochrome reductase, cytochrome oxidase. and their oxidative p!JosplJOl'.VJatiOtl adivity. amI photolytic capacity. These studies l'o\'oalod tho pl'osmwe of highly-significant numbers ofbiochomioally-act,ivc mitochondria am! smalJer, non-oxidativo partic!os, containing cytochromo reductase. Both pal't,iclc types were distinet from ehlol'oplasts, or, ehloroplast fragments. The onzyme clistl'iblltiollS almost, identical with those found for e{,jojnted leaves rovealing basic similarity bohvcen dark- and light·grown leaf coils.