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RESEARCH ARTICLE
Contents Vol 59(6)

The effect of endophyte on the performance of irrigated perennial ryegrasses in subtropical Australia

K. F. Lowe A C , T. M. Bowdler A , D. E. Hume B , N. D. Casey A and B. A. Tapper B
+ Author Affiliations
- Author Affiliations

A Department of Primary Industries and Fisheries, Mutdapilly Research Station, MS 825, Peak Crossing, Qld 4306, Australia.

B AgResearch, Grasslands Research Centre, Private Bag 11008, Palmerston North 4442, New Zealand.

C Corresponding author. Email: kevin.lowe@dpi.qld.gov.au

Australian Journal of Agricultural Research 59(6) 567-577 https://doi.org/10.1071/AR08019
Submitted: 13 October 2007  Accepted: 13 February 2008   Published: 10 June 2008

Abstract

The effect of fungal endophyte (Neotyphodium lolii) infection on the performance of perennial ryegrass (Lolium perenne) growing under irrigation in a subtropical environment was investigated. Seed of 4 cultivars, infected with standard (common toxic or wild-type) endophyte or the novel endophyte AR1, or free of endophyte (Nil), was sown in pure swards, which were fertilised with 50 kg N/ha.month. Seasonal and total yield, persistence, and rust susceptibility were assessed over 3 years, along with details of the presence of endophyte and alkaloids in plant shoots.

Endophyte occurrence in tillers in both the standard and AR1 treatments was above 95% for Bronsyn and Impact throughout and rose to that level in Samson by the end of the second year. Meridian AR1 only reached 93% while, in the standard treatment, the endophyte had mostly died before sowing. Nil endophyte treatments carried an average of ~0.6% infection throughout.

Infection of the standard endophyte was associated with increased dry matter (DM) yields in all 3 years compared with no endophyte. AR1 also significantly increased yields in the second and third years. Over the full 3 years, standard and AR1 increased yields by 18% and 11%, respectively. Infection with both endophytes was associated with increased yields in all 4 seasons, the effects increasing in intensity over time. There was 27% better persistence in standard infected plants compared with Nil at the end of the first year, increasing to 198% by the end of the experiment, while for AR1 the improvements were 20 and 134%, respectively. The effect of endophyte on crown rust (Puccinia coronata) infection was inconsistent, with endophyte increasing rust damage on one occasion and reducing it on another. Cultivar differences in rust infection were greater than endophyte effects.

Plants infected with the AR1 endophyte had no detectable ergovaline or lolitrem B in leaf, pseudostem, or dead tissue. In standard infected plants, ergovaline and lolitrem B were highest in pseudostem and considerably lower in leaf. Dead tissue had very low or no detectable ergovaline but high lolitrem B concentrations. Peramine concentration was high and at similar levels in leaf and pseudostem, but not detectable in dead material. Concentration was similar in both AR1 and standard infected plants.

Endophyte presence appeared to have a similar effect in the subtropics as has been demonstrated in temperate areas, in terms of improving yields and persistence and increasing tolerance of plants to stress factors.

Additional keywords: novel endophyte, alkaloids, yield, persistence, ergovaline, Lolitrem B, Peramine.


Acknowledgments

The authors thank Mr Dave Schofield and the staff at the Gatton Research Station for the day-to-day management of the experiment, Mrs Sandra Nolan for the botanical composition assessments, Mr Wayne Simpson and Ms Anouck de Bonth for endophyte detection, and Mrs Elizabeth Davies and Ms Amy Watson for alkaloid analysis.


References


Adcock RA, Hill NS, Boerma HR, Ware GO (1997) Sample variation and resource allocation for ergot alkaloid characterization in endophyte-infected tall fescue. Crop Science 37, 31–35. open url image1

Ball OJP, Barker GM, Prestidge RA, Lauren DR (1997a) Distribution and accumulation of the alkaloid peramine in Neotyphodium lolii-infected perennial ryegrass. Journal of Chemical Ecology 23, 1419–1434.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Ball OJP, Barker GM, Prestidge RA, Sprosen JM (1997b) Distribution and accumulation of the mycotoxin lolitrem B in Neotyphodium lolii-infected perennial ryegrass. Journal of Chemical Ecology 23, 1435–1449.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Barker DJ , Hume DE , Quigley PE (1997) Negligible physiological responses to water deficit in endophyte-infected and uninfected perennial ryegrass. In ‘Neotyphodium/grass interactions’. (Eds CW Bacon, NS Hill) pp. 137–139. (Plenum Press: New York)

Bluett SJ, Thom ER, Clark DA, Macdonald KA, Minneé EMK (2005a) Effects of perennial ryegrass infected with either AR1 or wild endophyte on dairy production in the Waikato. New Zealand Journal of Agricultural Research 48, 197–212. open url image1

Bluett SJ, Thom ER, Clark DA, Waugh CD (2005b) Effects of a novel ryegrass endophyte on pasture production, dairy cow milk production and calf liveweight gain. Australian Journal of Experimental Agriculture 45, 11–19.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Bouton J , Easton S (2005) Endophytes in forage cultivars. In ‘Neotyphodium in cool-season grasses’. (Eds CA Roberts, CP West, DE Spiers) pp. 327–340. (Blackwell Publishing: Ames, IA)

Christensen MJ (1996) Antifungal activity in grasses infected with Acremonium and Epichloë endophytes. Australasian Plant Pathology 25, 186–191.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Clay K (1990) The impact of parasitic and mutualistic fungi on competitive interactions among plants. In ‘Perspective on plant competition’. pp. 391–412. (Academic Press: New York)

Cooper B , Hume D , Panckhurst K , Popay A , Lyons T (2006) The role of perennial ryegrass endophyte in Italian ryegrass. In ‘Breeding for success: diversity in action. Proceedings of the Australasian Plant Breeding Conference’. (Ed. C Mercer) pp. 526–530. (New Zealand Grassland Association: Dunedin)

Crush JR, Popay AJ, Waller J (2004) Effect of different Neotyphodium endophytes on root distribution of a perennial ryegrass (Lolium perenne L.) cultivar. New Zealand Journal of Agricultural Research 47, 345–349. open url image1

Cunningham PJ, Foot JZ, Reed KFM (1993) Perennial ryegrass (Lolium perenne) endophyte (Acremonium lolii) relationships: the Australian experience. Agriculture, Ecosystems & Environment 44, 157–168.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Davies E , Lane GA , Latch GCM , Tapper BA , Garthwaite I , Towers NR , Fletcher LR , Pownall DB (1993) Alkaloid concentrations in field-grown synthetic perennial ryegrass endophyte associations. In ‘Proceedings of the Second International Symposium on Acremonium/Grass Interactions’. (Eds DE Hume, GCM Latch, HS Easton) pp. 72–76. (AgResearch: Palmerston North, NZ)

Di Menna ME, Mortimer PH, Prestidge RA, Hawkes AD, Sprosen JM (1992) Lolitrem B concentrations, counts of Acremonium lolii hyphae, and the incidence of ryegrass staggers in lambs on plots of A. lolii-infected perennial ryegrass. New Zealand Journal Agricultural Research 35, 211–217. open url image1

Fletcher LR (1999) “Non-toxic” endophytes in ryegrass and their effect on livestock health and production. In ‘Ryegrass endophyte: an essential New Zealand symbiosis’. Grassland Research and Practice Series 7. (Eds DR Woodfield, C Matthew) pp. 133–139. (New Zealand Grassland Association: Palmerston North)

Fletcher LR , Lane GA , Baird DB (2001) Seasonal variations of alkaloid concentrations in two perennial ryegrass – endophyte associations. In ‘Fourth International Neotyphodium/Grass Interactions Symposium’. Soest, Germany. pp. 535–541. (Universität – Gesamthochschule Paderborn: Abteilung Soest, Germany)

Fletcher LR , Sutherland BL , Fletcher CG (1999) The impact of endophyte on the health and productivity of sheep grazing ryegrass based pastures. In ‘Ryegrass endophyte: an essential New Zealand symbiosis’. Grassland Research and Practice Series 7. (Eds DR Woodfield, C Matthew) pp. 11–17. (New Zealand Grassland Association: Palmerston North)

Francis SM, Baird DB (1989) Increase in the proportion of endophyte-infected perennial ryegrass plants in overdrilled pastures. New Zealand Journal of Agricultural Research 32, 437–440. open url image1

Gallagher RT, Hawkes AD, Stewart JM (1985) Rapid determination of the neurotoxin lolitrem B in perennial ryegrass by high-performance liquid chromatography with fluorescence detection. Journal of Chromatography. A 321, 217–226.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Genstat 8 Committee (2005) ‘GenStat Release 8 Reference Manual. Software version 8.’ (Oxford University Press: Oxford, UK)

Gwinn KD, Collins-Shepard MH, Reddick BB (1991) Tissue print-immunoblot, an accurate method for the detection of Acremonium coenophialum in tall fescue. Phytopathology 81, 747–748.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Hahn H, Huth W, Schoberlein W, Diepenbrock W (2003) Detection of the endophytic fungi in Festuca spp. by means of tissue print immunoassay. Plant Breeding 122, 217–222.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Hawkes AD , Sprosen JM , Armstrong JA , Laboyrie D , Hopper JI (1995) Influence of weather conditions on lolitrem B pasture levels and ryegrass staggers incidence. In ‘Toxinology and Food Safety Research Report’. (Ed. L Garthwaite) pp. 5–7. (AgResearch: Hamilton, New Zealand)

Hume DE , Barker DJ (2005) Growth and management of endophytic grasses in pastoral agriculture. In ‘Neotyphodium in cool-season grasses’. (Eds CA Roberts, CP West, DE Spiers) pp. 201–226. (Blackwell Publishing: Ames, IA)

Hume DE , Popay AJ , Cooper BM , Eerens JPJ , Lyons TB , Pennell CGL , Tapper BA , Latch GCM , Baird DB (2004) Effect of a novel endophyte on the productivity of perennial ryegrass (Lolium perenne) in New Zealand. In ‘Proceedings of the 5th International Symposium on Neotyphodium/Grass Interactions’. Fayetteville, Arkansas. (Eds R Kallenbach, CJ Rosenkranz, TR Lock) p. Poster 313. (University of Arkansas: Fayetteville, AR)

Keogh RG, Tapper BA, Fletcher RH (1996) Distributions of the fungal endophyte Acremonium lolii, and of the alkaloids lolitrem B and peramine, within perennial ryegrass. New Zealand Journal of Agricultural Research 39, 121–127. open url image1

Lane GA , Tapper BA , Davies E , Hume DE , Latch GCM , Barker DJ , Easton HS , Rolston MP (1997) Effect of growth conditions on alkaloid concentrations in perennial ryegrass naturally infected with endophyte. In ‘Neotyphodium/grass interactions. Proceedings of the Third International Symposium on Neotyphodium/Grass Interactions’. (Eds CW Bacon, NS Hill) pp. 179–182. (Plenum Press: New York, Athens, GA)

Latch GCM (1994) The influence of Acremonium endophytes on perennial grass improvement. New Zealand Journal of Experimental Agriculture 37, 311–318. open url image1

Latch GCM, Christensen MJ, Samuels GJ (1984) Five endophytes of Lolium and Festuca in New Zealand. Mycotaxon 20, 535–550. open url image1

Latch GCM, Hunt WF, Musgrave DR (1985) Endophytic fungi affect growth of perennial ryegrass. New Zealand Journal of Agricultural Research 28, 165–168. open url image1

Launders T , Blumenthal M , Kemp H (1996) Perennial ryegrass and tall fescue – evaluation. In ‘NSW Agriculture Dairy Research Institute 1995/96’. (Ed. B Fulkerson) pp. 15–20. (NSW Agriculture: NSW, Australia)

Layton DL, Fletcher LR, Litherland AJ, Scannell MG, Sprosen J, Hoogendoorn CJ, Lambert MG (2004) Effect of ergot alkaloids on liveweight gain and urine lysergol level in ewe hoggets and heifers. Proceedings of the New Zealand Society of Animal Production 64, 192–196. open url image1

Lowe KF, Bowdler TM (1984) The performance of temperate and tropical grasses under two irrigation frequencies. Tropical Grasslands 18, 46–55. open url image1

Lowe KF, Bowdler TM (1995) Growth, persistence, and rust sensitivity of irrigated, temperate perennial grasses in the Queensland subtropics. Australian Journal of Experimental Agriculture 35, 571–578.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Lowe KF , Bowdler TM , Casey ND (2004) ‘Evaluation of temperate species in the subtropics – 2003.’ Department of Primary industries Information Series QI0403. (Department of Primary Industries: Brisbane, Qld)

Lowe KF, Bowdler TM, Casey ND, Lowe SA, White JA, Pepper PM (2008a) Evaluating temperate species in the subtropics. 2. Perennial grasses. Tropical Grasslands 42, 1–26. open url image1

Lowe KF, Bowdler TM, Casey ND, Moss RJ (1999a) Performance of temperate perennial pastures in the Australian subtropics. 1. Yield, persistence and pasture quality. Australian Journal of Experimental Agriculture 39, 663–676.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Lowe KF, Bowdler TM, Casey ND, Moss RJ (1999b) Performance of temperate perennial pastures in the Australian subtropics. 2. Milk production. Australian Journal of Experimental Agriculture 39, 677–683.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Lowe KF, Callow NM, Bowdler TM, Lowe SA, White JA, Gobius N (2008b) The performance of irrigated mixtures of tall fescue, ryegrass and white clover in subtropical Australia. 1. The effect of sowing mixture combinations, nitrogen and oversowing on establishment, productivity, botanical composition and persistence. Tropical Grasslands in press 42, open url image1

Malinowski DP, Belesky DP (2000) Adaptations of endophyte-infected cool-season grasses to environmental stresses: mechanisms of drought and mineral stress tolerance. Crop Science 40, 923–940. open url image1

McDonald G , Noske A , Van Heeswijck R , Frost WE (1993) The role of the perennial ryegrass endophyte in the management of pasture pests in south eastern Australia. In ‘Proceedings of the 6th Australasian Conference on Grassland Invertebrate Ecology’. (Ed. RA Prestidge) pp. 295–302. (AgResearch: Hamilton, New Zealand)

Northcote KH (1971) ‘A factual key for the recognition of Australian soils.’ 3rd edn (Rellim Technical Publications: Glenside, S. Aust.)

Pennell CGL, Popay AJ, Ball OJ-P, Hume DE, Baird DB (2005) Occurrence and impact of pasture mealybug (Balanococcus poae) and root aphid (Aploneura lentisci) on ryegrass (Lolium spp.) with and without infection by Neotyphodium fungal endophytes. New Zealand Journal of Agricultural Research 48, 329–337. open url image1

Popay AJ , Bonos SA (2005) Biotic responses in endophytic grasses. In ‘Neotyphodium in cool-season grasses’. (Eds CA Roberts, CP West, DE Spiers) pp. 163–185. (Blackwell Publishing, Ames, IA)

Popay AJ , Hume DE , Baltus JG , Latch GCM , Tapper BA , Lyons TB , Cooper BM , Pennell CG , Eerens JPJ , Marshall SL (1999) Field performance of perennial ryegrass (Lolium perenne) infected with toxin-free fungal endophytes (Neotyphodium spp.). In ‘Ryegrass endophyte: an essential New Zealand symbiosis’. Grassland Research and Practice Series 7. (Eds DR Woodfield, C Matthew) pp. 113–122.

Prestidge RA , di Menna ME , van der Zijpp S , Badan D (1985) Ryegrass content, Acremonium endophyte and Argentine stem weevil in pastures in the volcanic plateau. Proceedings of the 38th New Zealand Weed and Pest Control Conference 38, 41–44.

Quigley PE (2000) Effects of Neotyphodium lolii infection and sowing rate of perennial ryegrass (Lolium perenne) on the dynamics of ryegrass/subterranean clover (Trifolium subterraneum) swards. Australian Journal of Agricultural Research 51, 47–56.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Read J , McGowan A , Reed K , Ward G , Lowe K , Kaehne I (1990) Research in perennial pastures for the Australian dairy industry. In ‘Feedbase 2000: A Workshop to Determine the Priorities for Research into Soils, Pastures and Fodder Crops’. (Eds B Bartsch, W Mason) pp. 35–43. (CSIRO and Dairy Research and Development Corporation: Melbourne, Vic.)

Reed KFM, Walsh JR, Cross PA, McFarlane NM, Sprague MA (2004) Ryegrass endophyte (Neotyphodium lolii) alkaloids and mineral concentrations in perennial ryegrass (Lolium perenne) from southwest Victorian pasture. Australian Journal of Experimental Agriculture 44, 1185–1194.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Schmidt SP, Osborn TG (1993) Effects of endophyte-infected tall fescue on animal performance. Agriculture, Ecosystems & Environment 44, 233–262.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Spiering MJ, Davies E, Tapper BA, Schmid J, Lane GA (2002) Simplified extraction of ergovaline and peramine for analysis of tissue distribution in endophyte-infected grass tillers. Journal of Agricultural and Food Chemistry 50, 5856–5862.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Stace HCT , Hubble GD , Brewer R , Northcote KH , Sleeman JR , Mulcahy MJ , Hallsworth EG (1972) ‘A handbook of Australian soils.’ (Rellim Technical Publications: Glenside, S. Aust.)

Tor-Agbidye J, Blythe LL, Craig AM (2001) Correlation of endophyte toxins (ergovaline and lolitrem B) with clinical disease: fescue foot and perennial ryegrass staggers. Veterinary and Human Toxicology 43, 140–146.
| PubMed |
open url image1

Valentine SC , Bartsch BD , Boyce KG , Mathison MJ , Newbery TR (1993) ‘Incidence and significance of endophyte in perennial grass seed lines’. Final report on project DAS33 prepared for the Dairy Research and Development Corporation. (SARDI: Adelaide, S. Aust.)

Vincelli P, Powell AJ (1991) Reaction of perennial ryegrass varieties to red thread, 1990. Biological and Cultural Tests for Control of Plant Disease 6, 102. open url image1

Watson RH, Keogh RG, McDonald MF (1999) Ewe reproductive performance and growth rate of suckling-lambs on endophyte-infected perennial ryegrass pasture. Grassland Research and Practice Series New Zealand Grassland Association 7, 19–26. open url image1

Wheatley WM (1998) Management, environmental and livestock interactions impact on perennial ryegrass/Neotyphodium/livestock associations. In ‘Toxic plants and other natural toxicants’. (Eds T Garland, AC Barr) pp. 45–48. (CAB International: Oxon, UK)

Wheatley WM , Nicol HI , Hunt ER , Nikandrow A , Cother N (2000) An association between perennial ryegrass endophyte, a leafspot caused by (Pyrenophora semeniperda) and preferential grazing by sheep. In ‘The 3rd International Conference on Harmful and Beneficial Microorganisms in Grasslands, Pastures and Turf’. (Eds VH Paul, PD Dapprich) pp. 71–75. (Universität Paderborn: Soest, Germany)

Woodburn OJ , Walsh JR , Foot JZ , Heazlewood PG (1993) Seasonal ergovaline concentrations in perennial ryegrass cultivars of differing endophyte status. In ‘Proceedings of the Second International Symposium on Acremonium/Grass Interactions’. Palmerston North, NZ. (Eds DE Hume, GCM Latch, HS Easton) pp. 100–102. (AgResearch)