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RESEARCH ARTICLE
Contents Vol 59(9)

Genetic analysis of pod and seed resistance to pea weevil in a Pisum sativum × P. fulvum interspecific cross

O. M. Byrne A C D , D. C. Hardie B , T. N. Khan B , J. Speijers B and G. Yan A
+ Author Affiliations
- Author Affiliations

A School of Plant Biology, Faculty of Natural and Agricultural Sciences, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.

B Department of Agriculture and Food, Western Australia, 3 Baron-Hay Court, South Perth, WA 6151, Australia.

C Centre for Legumes in Mediterranean Agriculture, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.

D Corresponding author. Email: oonagh.byrne@uwa.edu.au

Australian Journal of Agricultural Research 59(9) 854-862 https://doi.org/10.1071/AR07353
Submitted: 25 September 2007  Accepted: 3 June 2008   Published: 26 August 2008

Abstract

Interspecific populations derived from crossing cultivated field pea, Pisum sativum, with the wild pea relative, Pisum fulvum, were scored for pod and seed injury caused by the pea weevil, Bruchus pisorum. Pod resistance was quantitatively inherited in the F2 population, with evidence of transgressive segregation. Heritability of pod resistance between F2 and F3 generations was very low, suggesting that this trait would be difficult to transfer in a breeding program. Seed resistance was determined for the F2 population by testing F3 seed tissues of individual F2 plants and pooling data from seed reaction for each F2 plant (inferred F2 genotype). Segregation for seed resistance in the F2 population of the cross Pennant/ATC113 showed a trigenic mode of inheritance, with additive effects and dominant epistasis towards susceptibility. Seed resistance was conserved over consecutive generations (F2 to F5) and successfully transferred to a new population by backcross introgression. Seed resistance in the backcross introgressed population segregated in a 63 : 1 ratio, supporting the three-gene inheritance model. It is proposed that complete resistance to pea weevil is controlled by three major recessive alleles assigned pwr1, pwr2, and pwr3, and complete susceptibility by three major dominant alleles assigned PWR1, PWR2, and PWR3. It is recommended that large populations (>300 F2 plants) would be required to effectively transfer these recessive alleles to current field pea cultivars through hybridisation and repeated backcrossing.

Additional keywords: interspecific hybridisation, trigenic inheritance, antibiosis, Bruchus pisorum.


Acknowledgments

We are most grateful to H. Collie and R. Flores Vargas for their technical expertise. This research was financed by the Grains Research and Development Corporation (UWA209 and UWA314) at the Centre for Legumes in Mediterranean Agriculture, The University of Western Australia. The support of the Department of Agriculture and Food (WA), the Australian Research Council, and Dardin Agri Holdings (Australia) Pty Ltd is also gratefully acknowledged.


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