Register      Login
Crop and Pasture Science Crop and Pasture Science Society
Plant sciences, sustainable farming systems and food quality
Shopping Cart: ( empty )
You are here: Home > Journals > CP > AR07353
RESEARCH ARTICLE
Contents Vol 59(9)

Genetic analysis of pod and seed resistance to pea weevil in a Pisum sativum × P. fulvum interspecific cross

O. M. Byrne A C D , D. C. Hardie B , T. N. Khan B , J. Speijers B and G. Yan A
+ Author Affiliations
- Author Affiliations

A School of Plant Biology, Faculty of Natural and Agricultural Sciences, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.

B Department of Agriculture and Food, Western Australia, 3 Baron-Hay Court, South Perth, WA 6151, Australia.

C Centre for Legumes in Mediterranean Agriculture, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.

D Corresponding author. Email: oonagh.byrne@uwa.edu.au

Australian Journal of Agricultural Research 59(9) 854-862 https://doi.org/10.1071/AR07353
Submitted: 25 September 2007  Accepted: 3 June 2008   Published: 26 August 2008

Abstract

Interspecific populations derived from crossing cultivated field pea, Pisum sativum, with the wild pea relative, Pisum fulvum, were scored for pod and seed injury caused by the pea weevil, Bruchus pisorum. Pod resistance was quantitatively inherited in the F2 population, with evidence of transgressive segregation. Heritability of pod resistance between F2 and F3 generations was very low, suggesting that this trait would be difficult to transfer in a breeding program. Seed resistance was determined for the F2 population by testing F3 seed tissues of individual F2 plants and pooling data from seed reaction for each F2 plant (inferred F2 genotype). Segregation for seed resistance in the F2 population of the cross Pennant/ATC113 showed a trigenic mode of inheritance, with additive effects and dominant epistasis towards susceptibility. Seed resistance was conserved over consecutive generations (F2 to F5) and successfully transferred to a new population by backcross introgression. Seed resistance in the backcross introgressed population segregated in a 63 : 1 ratio, supporting the three-gene inheritance model. It is proposed that complete resistance to pea weevil is controlled by three major recessive alleles assigned pwr1, pwr2, and pwr3, and complete susceptibility by three major dominant alleles assigned PWR1, PWR2, and PWR3. It is recommended that large populations (>300 F2 plants) would be required to effectively transfer these recessive alleles to current field pea cultivars through hybridisation and repeated backcrossing.

Additional keywords: interspecific hybridisation, trigenic inheritance, antibiosis, Bruchus pisorum.


Acknowledgments

We are most grateful to H. Collie and R. Flores Vargas for their technical expertise. This research was financed by the Grains Research and Development Corporation (UWA209 and UWA314) at the Centre for Legumes in Mediterranean Agriculture, The University of Western Australia. The support of the Department of Agriculture and Food (WA), the Australian Research Council, and Dardin Agri Holdings (Australia) Pty Ltd is also gratefully acknowledged.


References


ACTA (1966) Biology and control of the pea weevil in Lotein, Hupeh, China. Acta Entomologica Sinica 15, 288–293. open url image1

Adjadi O, Singh BB, Singh SR (1985) Inheritance of bruchid resistance in cowpea. Crop Science 25, 740–742. open url image1

Ben-Ze’ev N, Zohary D (1973) Species relationships in the genus Pisum L. Israel Journal of Botany 22, 73–91. open url image1

Birks PR (1965) Pea weevil—a new insect of peas in South Australia. Journal of Agriculture , 156–160. open url image1

Brindley TA (1933) Some notes on the biology of the pea weevil Bruchus pisorum L. (Coleoptera, Bruchidae) at Moscow, Idaho. Journal of Economic Entomology 26, 1058–1062. open url image1

Brindley TA , Chamberlin JC , Schopp R (1956) The pea weevil and methods for its control. U.S. Department of Agriculture – Farmers’ Bulletin No. 1971. pp. 1–24.

Byrne OMT (2005) Incorporation of pea weevil resistance from wild pea (Pisum fulvum) into cultivated field pea (Pisum sativum). PhD Thesis, The University of Western Australia, WA, Australia.

Clement SL, Hardie DC, Elberson LR (2002) Variation among accessions of Pisum fulvum for resistance to pea weevil. Crop Science 42, 2167–2173. open url image1

Dodds KS, Matthews P (1966) Neoplastic pod in the pea. Journal of Heredity 57, 83–88. open url image1

Doss RP, Oliver JE, Proebsting WM, Potter SW, Kuy S, Clement SL, Williamson RT, Carney JR, DeVilbiss ED (2000) Bruchins: insect-derived plant regulators that stimulate neoplasm formation. Proceedings of the National Academy of Sciences of the United States of America 97, 6218–6223.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Errico A, Conicella C, De Martino T, Ercolano R, Monti LM (1996) Chromosome reconstructions in Pisum sativum through interspecific hybridisation with P. fulvum. Journal of Genetics & Breeding 50, 309–313. open url image1

Falconer (1981) ‘Introduction to quantitative genetics.’ (Longman Group Limited: New York)

Fondevilla S, Torres AM, Moreno MT, Rubiales D (2007) Identification of a new gene for resistance to powdery mildew in Pisum fulvum, a wild relative of pea. Breeding Science 57, 181–184.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Hardie DC (1992) Resistance to the pea weevil in Pisum species. PhD Thesis, The University of Adelaide, SA, Australia.

Hardie DC, Baker GJ, Marshall DR (1995) Field screening of Pisum accessions to evaluate their susceptibility to the pea weevil (Coleoptera: Bruchidae). Euphytica 84, 155–161.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Hardie DC, Clement SL (2001) Development of bioassays to evaluate wild pea germplasm for resistance to pea weevil (Coleoptera: Bruchidae). Crop Protection 20, 517–522.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Hoey BK, Crowe KR, Jones VM, Polans NO (1996) A phylogenetic analysis of Pisum based on morphological characters, and allozyme and RAPD markers. Theoretical and Applied Genetics 92, 92–100.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Kornegay JL, Cardona C (1991) Inheritance of resistance to Acanthoscelides obtectus in a wild common bean accession crossed to commercial bean cultivars. Euphytica 52, 103–111. open url image1

Lawrence MJ , Jinks JL (1973) Quantitative genetics. In ‘Practical genetics’. (Ed. PM Sheppard) pp. 86–129. (Blackwell Scientific Publications: Oxford, UK)

Marzo F, Aguirre A, Castiella MV, Alonso R (1997) Fertilization effects of phosphorus and sulfur on chemical composition of seeds of Pisum sativum L. and relative infestation by Bruchus pisorum L. Journal of Agricultural and Food Chemistry 45, 1829–1833.
| Crossref | GoogleScholarGoogle Scholar | open url image1

McCullagh P , Nelder JA (1989) ‘Generalized linear models.’ (Chapman and Hall: London)

Muehlbauer FJ , Kaiser WJ (1994) Using host plant resistance to manage biotic stresses in cool season legumes. In ‘Expanding the production and use of cool season food legumes’. (Eds FJ Muehlbauer, WJ Kaiser) pp. 233–246. (Kluwer Academic Publishers: Dordrecht, The Netherlands)

Newman LJ (1932) The pea weevil (Bruchus pisorum, Linn). Journal of Agriculture, Western Australia , 297–300. open url image1

Nuttal VW, Lyall LH (1964) Inheritance of neoplastic pods in pea. Journal of Heredity 55, 184–186. open url image1

Nyquist WE (1991) Estimation of heritability and prediction of selection response in plant populations. Critical Reviews in Plant Sciences 103, 235–322. open url image1

Ochatt SJ, Benabdelmouna A, Marget P, Aubert G, Moussy F, Pontecaille C, Jacas L (2004) Overcoming hybridisation barriers between pea and some of its wild relatives. Euphytica 137, 353–359.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Parlevliet JE (1992) Selecting components of partial resistance. In ‘Plant breeding in the 1990s’. (Eds HT Stalker, JP Murphy) pp. 281–302. (CAB International: Wallingford, UK)

Pesho GR, Muehlbauer FJ, Harberts WH (1977) Resistance of pea introductions to the pea weevil. Journal of Economic Entomology 70, 30–33. open url image1

Pintureau B, Gerding M, Cisternas E (1999) Description of three new species of Trichogrammatidae (Hymenoptera) from Chile. Canadian Entomologist 131, 53–63. open url image1

Redden RJ (1983) The inheritance of seed resistance to Callosobruchus maculatus F. in cowpea (Vigna unguiculata L. Walp.). II. Analyses of percentage emergence and emergence periods of bruchids in F4 seed generation of two reciprocal crosses. Australian Journal of Agricultural Research 34, 697–705.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Redden RJ, McGuire J (1983) The genetic evaluation of bruchid resistance in seed of cowpea. Australian Journal of Agricultural Research 34, 707–715.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Reyes-Valdés MH (2000) A model for marker-based selection in gene introgression breeding programs. Crop Science 40, 91–98. open url image1

Robinson RA (1996) Techniques. In ‘Return to resistance’. (Ed. RA Robinson) pp. 277–303. (agAccess: Davis, CA)

Rusoke DG, Fatunla T (1987) Inheritance of pod and seed resistance to the cow-pea seed beetle (Callosobruchus maculatus Fabr.). Journal of Agricultural Science, Cambridge 108, 655–660. open url image1

Simmonds MSJ, Blaney WM, Birch ANE (1989) Legume seeds: the defence of a wild and cultivated species of Phaseolus against attack by bruchid beetles. Annals of Botany 63, 177–184. open url image1

Skaife SH (1918) Pea and bean weevils. Bulletin of the Department of Agriculture, Union of South Africa. pp. 1–32.

Smartt J (1984) Evolution of grain legumes. I. Mediterranean pulses. Experimental Agriculture 20, 275–296. open url image1

Snedecor GW , Cochran WG (1980) ‘Statistical methods.’ (The Iowa State University Press: Ames, IA)

Tanksley SD, Young ND, Paterson AH, Bonierbale MW (1989) RFLP mapping in plant breeding: new tools for an old science. Bio/Technology 7, 257–264.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Wright S (1968) ‘Genetic and biometric foundations.’ (The University of Chicago Press: Chicago, IL)

Wroth JM (1998) Possible role for wild genotypes of Pisum spp. to enhance ascochyta blight resistance in pea. Australian Journal of Experimental Agriculture 38, 469–479.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Xu H, Qiang S, Han Z, Guo J, Huang Z , et al. (2006) The status and causes of alien species invasion in China. Biodiversity and Conservation 15, 2893–2904.
| Crossref | GoogleScholarGoogle Scholar | open url image1