Free Standard AU & NZ Shipping For All Book Orders Over $80!
Register      Login
Crop and Pasture Science Crop and Pasture Science Society
Plant sciences, sustainable farming systems and food quality
Shopping Cart: ( empty )
You are here: Home > Journals > CP > AR06300
RESEARCH ARTICLE
Contents Vol 58(5)

Genotypic variability in sequence and expression of HVA1 gene in Tibetan hulless barley, Hordeum vulgare ssp. vulgare, associated with resistance to water deficit

Gang Qian A B C D , Zhaoxue Han A C D , Tao Zhao A C , Guangbing Deng A , Zhifen Pan A and Maoqun Yu A E
+ Author Affiliations
- Author Affiliations

A Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China.

B Department of Biology, Zunyi Medical College, Zunyi 563003, China.

C Graduate University of the Chinese Academy of Sciences, Beijing 100039, China.

D The first two authors have contributed equally to this paper.

E Corresponding author. Email: yumq@cib.ac.cn

Australian Journal of Agricultural Research 58(5) 425-431 https://doi.org/10.1071/AR06300
Submitted: 6 September 2006  Accepted: 2 March 2007   Published: 11 May 2007

Abstract

Late embryogenesis abundant (LEA) proteins are thought to protect against water stress in plants. Characteristics of sequence and expression of barley gene HVA1, a member of LEA group 3 protein, were investigated in hulless barley (Hordeum vulgare ssp. vulgare), associated with phenotypically diverse drought-tolerant genotypes. Sensitive and tolerant genotypes were identified from Tibetan populations of cultivated hulless barley, based on scores of water loss rate (WLR), maldondialdehyde (MDA), and proline content. The results indicated that lower MDA contents, lower scores of WLR, and higher proline contents were associated with drought-tolerant genotypes in hulless barley. Notably, differential trends of expression patterns were detected among the selected contrasting genotypes, depending on the duration of dehydration stress. The HVA1 gene tended to respond earlier in the tolerance (after 2 h) compared with sensitive genotypes (after 4 h). Results of quantitative real-time PCR indicated that the relative level of HVA1 expression was always higher in tolerant genotypes, rapidly increasing at the earlier stages (after 2–4 h of dehydration). However, HVA1 expressions of sensitive genotypes had a fast increase from 8 to 12 h of stress. Variable numbers of the 11-amino-acid-motif in LEA3 proteins were not consistent with the lines of drought resistance in hulless barley. Molecular characteristic of LEA3 protein in tolerant lines existed in the consistency of Gln32, Arg33, and Ala195 in Tibetan hulless barley. The present study may indicate that the differential HVA1 gene has a functional role in the dehydration tolerance in hulless barley. The authors suggested that the observed variability in sequence and expression of HVA1 could be related to the diverse drought-tolerant genotypes in crops.

Additional keywords: water loss rate, lipid peroxidation, proline, quantitative polymerase chain reaction.


Acknowledgments

The authors greatly acknowledge the financial support of this project by the National High Technology Research and Development Program of China (No. 2003AA207100), National Natural Sciences Foundation of China (No. 30270830), and the Key Project of Scientific and Technological Innovation of the Chinese Academy of Sciences (No. KSCX2-SW-304).


References


Araus JL, Slafer GA, Reynolds MP, Royo C (2002) Plant breeding and drought in C3 cereals: what should we breed for? Annals of Botany 89, 925–940.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Aspinall D , Paleg LG (1981) Proline accumulation: physiological aspects. In ‘The physiology and biochemistry of drought resistance in plants’. (Eds L Paleg, D Aspinall) pp. 215–228. (Academic Press: Sydney)

Bartels D , Souer E (2004) Molecular responses of higher plants to dehydration. In ‘Plant responses to abiotic stress’. (Eds H Hirt, K Shinozaki) pp. 9–38. (Springer-Verlag: Berlin)

Bates LS, Waldren RP, Teare JD (1973) Rapid determination of free proline for water-stress studies. Plant and Soil 39, 205–207.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Bor M, Ozdemir F, Turkan I (2003) The effect of salts tress on lipid peroxidation and antioxidants in leaves of sugar beet Beta vulgaris L. and wild beet Beta maritima L. Plant Science 164,
| Crossref |
open url image1

Bruce WB, Edmeades GO, Barker TC (2002) Molecular and physiological approaches to maize improvement for drought tolerance. Journal of Experimental Botany 53, 13–25.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Cellier F, Conejero G, Breitler JC, Casse F (1998) Molecular and physiological responses to water deficit in drought-tolerant and drought-sensitive sunflower lines (Helianthus annuus L.): accumulation of dehydrin transcripts correlates with tolerance. Plant Physiology 116, 319–328.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Chandra Babu R, Zhang JX, Blum A, David Ho TH, Wu R, Nguyen HT (2004) HVA1, a LEA gene from barley confers dehydration in transgenic rice (Oryza sativa L.) via cell membrane protection. Plant Science 166, 855–862.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Choi DW, Zhu B, Close TJ (1999) The barley (Hordeum vulgare L.) dehydrin multigene family: sequences, allelic types, chromosome assignments, and expression characteristics of 11 Dhn genes of cv Dicktoo. Theoretical and Applied Genetics 98, 1234–1247.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Clarke JM, McCaig TN (1982) Evaluation of techniques for drought resistance in wheat. Crop Science 22, 503–506. open url image1

Close TJ , Choi DW , Venegas M , Salvi S , Tuberosa R , Ryabushkina N , Turuspekov Y , Nevo E (2000) Allelic variation in wild and cultivated barley at the Dhn4 locus, which encodes a major drought-induced and seed protein, DHN4. In ‘8th International Barley Genetics Symposium’. Adelaide, S. Aust. (Ed. S Logue)

Dure L III (1993) Structural motifs in LEA proteins of higher plants. In ‘Response of plants to cellular dehydration during environmental stress’. (Eds TJ Close, EA Bray) pp. 91–103. (American Society of Plant Physiologists: Rockville, MD)

Gal TZ, Glazer I, Koltai H (2004) An LEA group3 family member is involved in survival of C. elegans during exposure to stress. FEBS Letters 577, 21–26.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics 125, 189–198.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Hong B, Uknes SJ, Ho THD (1988) Cloning and characterization of a cDNA encoding a mRNA rapidly-induced by ABA in barley aleurone layers. Plant Molecular Biology 11, 495–506.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Jain M, Mathur G, Koul S, Sarin NB (2001) Ameliorative effects of proline on salt stress induced lipid peroxidation in cell lines of groundnut (Arachis hypogea L.). Plant Cell Reports 20, 463–468.
| Crossref |
open url image1

Jayaprakash TL, Ramamohan G, Krishnaprasad BT, Ganeshkumar , Prasad TG, Mathew MK, Udayakumar M (1998) Genotypic variability in differential expression of lea2 and lea3 genes and proteins in response to salinity stress in fingermillet (Eleusine coracana Gaertn) and rice (Oryza sativa L) seedlings. Annals of Botany 82, 513–522.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Kawasaki S, Borchert C, Deyholos M, Wang H, Brazille S, Kawai K, Galbraith D, Bohnert HJ (2001) Gene expression profiles during the initial phase of salt stress in rice. The Plant Cell 13, 889–905.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Krishnan M, Nguen HT, Burke JJ (1989) Heat shock protein synthesis and thermal tolerance in wheat. Plant Physiology 90, 140–145.
| PubMed |
open url image1

Labhilili M, Jourdier P, Gautier MF (1995) Characterization of cDNAs encoding Triticum durum dehydrins and their expression patterns in cultivars that differ in drought tolerance. Plant Science 112, 219–230.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Leon D (1993) A repeating 11-mer amino acid motif and plant desiccation. The Plant Journal 3, 393–369.
| Crossref | PubMed |
open url image1

Lopez CG, Banowetz GM, Peterson CJ, Kronstad WE (2003) Dehydrin expression and drought tolerance in seven wheat cultivars. Crop Science 43, 577–582. open url image1

Ludlow MM, Muchow RC (1990) A critical evaluation of traits for improving crop yields in water-limited environments. Advances in Agronomy 43, 107–153. open url image1

Mohsenzadeh S, Malboobi MA, Razavi K, Farrahi-Aschtiani S (2006) Physiological and molecular response of Aeluropus lagopoides (Poaceae) to water deficit. Environmental and Experimental Botany 56, 314–322.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15, 473–497.
| Crossref |
open url image1

Paquin R, Lechasseur P (1979) Observations sur une methode de dosage de la proline libre dans les extraits de plantes. Canadian Journal of Botany 57, 1851–1854. open url image1

Pelah D, Wang W, Altman A, Shoseyov O, Bartles D (1997) Differential accumulation of water stress-related proteins, sucrose synthase and soluble sugars in Populus species that differ in their water stress response. Physiologia Plantarum 99, 153–159.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Ramanjulu S, Bartels D (2002) Drought- and desiccation-induced modulation of gene expression in plants. Plant, Cell & Environment 25, 141–151.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Reid JL, Walker-Simmons MK (1993) Group 3 late embryogenesis abundant proteins in desiccation tolerant seedlings of wheat (Triticum aestivum L.). Plant Physiology 102, 125–131.
| PubMed |
open url image1

Ristic Z, Jenks MA (2002) Leaf cuticle and water loss in maize lines differing in dehydration avoidance. Journal of Plant Physiology 159, 645–651.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Sivamani E, Bahieldin A, Wraith JM, Al-Niemi T, Dyer WE, Ho T-HD, Qu RD (2000) Improved biomass productivity and water use efficiency under water deficit conditions in transgenic wheat constitutively expressing the barley HVA1 gene. Plant Science 155, 1–9.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Smith JAC , Griffiths H (1993) ‘Water deficits: plant responses from cell to community.’ pp. 1–332. (Bios Scientific Publishers: Oxford, UK)

Suprunova T, Krugman T, Fahima T, Chen G, Shams I, Korol A, Nevo E (2004) Differential expression of dehydrin genes in wild barly, Hordeum spontaneum, associated with resistance to water deficit. Plant, Cell & Environment 27, 1297–1308.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Turkan I, Bor M, Ozdemir F, Koca H (2005) Differential responses of lipid peroxidation and antioxidants in the leaves of drought-tolerant P. acutifolius Gray and drought-sensitive P. vulgaris L. subjected to polyethylene glycol mediated water stress. Plant Science 168, 223–231.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Uma S, Ravishankar KV, Prasad TG, Reid JL, Udayakumar M (1993) Abscisic acid responsive proteins induce salinity stress tolerance in fingermillet (Eleusine coracana Gaertn.). Current Science 65, 549–554. open url image1

Vierling E, Nguyen HT (1992) Heat-shock protein gene expression in diploid wheat genotypes differing in thermal tolerance. Crop Science 32, 370–377. open url image1

Xu D, Duan X, Wang B, Hong B, Ho THD, Wu R (1996) Expression of a late embryogenesis abundant protein gene, HVA1, from barley confers tolerance to water deficit and salt stress in transgenic rice. Plant Physiology 110, 249–257.
| PubMed |
open url image1

Zhu B, Choi DW, Fenton R, Close TJ (2000) Expression of the barley dehydrin multigene family and the development of freezing tolerance. Molecular and General Genetics 264, 145–153.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1