Accumulation and phosphatase-lability of organic phosphorus in fertilised pasture soils
Timothy S. George A B , Richard J. Simpson A , Paul A. Hadobas A , David J. Marshall A and Alan E. Richardson A CA CSIRO Plant Industry, PO Box 1600, Canberra, ACT 2601, Australia.
B Current address: Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, Scotland, UK.
C Corresponding author. Email: alan.richardson@csiro.au
Australian Journal of Agricultural Research 58(1) 47-55 https://doi.org/10.1071/AR06167
Submitted: 17 May 2006 Accepted: 12 September 2006 Published: 2 January 2007
Abstract
The accumulation and phosphatase-lability of organic P was investigated in soil taken from 3 pastures that had received contrasting fertiliser management over 8 years. The soils were either unfertilised or had received superphosphate either from 1994 to 1997 or applied annually to 2002. P-fertilisation led to an increase in both the inorganic and organic P content of the soils, but with differences in the distribution of organic P in various extractable pools. Fertilisation also affected the amount of organic P that was amenable to hydrolysis by a non-specific phosphatase. In particular, the amount of water-extractable organic P that was phosphatase-labile was greatest in soil that had received continuous fertiliser application. Despite improved phosphatase-lability of different organic P pools in the fertilised soils, transgenic Trifolium subterraneum L., which releases extracellular phytase, showed no consistent advantage in growth and P nutrition compared with either wild-type or azygous controls when grown in intact cores of soil. This indicates that organic P that accumulates with P-fertilisation is either not an effective substrate for transgenic plants that exude phytase or is equally available to transgenic and control plants.
Additional keywords: Alfisol, Aspergillus niger, subterranean clover, intact-cores, P-availability, phytate.
Acknowledgments
This research was supported by the Australian woolgrowers and the Australian Government through grants from Australian Wool Innovations Ltd. The authors thank Barry Smith and staff at the Ginninderra Experimental Station for technical assistance.
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