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RESEARCH ARTICLE
Contents Vol 58(4)

Effect of lamb age and electrical stimulation on the colour stability of fresh lamb meat

R. H. Jacob A D , M. F. D’Antuono A , G. M. Smith A , D. W. Pethick B and R. D. Warner C
+ Author Affiliations
- Author Affiliations

A Department of Agriculture and Food WA, 3 Baron-Hay Court, South Perth, WA 6151, Australia.

B School of Veterinary and Biomedical Science, Murdoch University, Murdoch, WA 6150, Australia.

C Department of Primary Industries, 600 Sneydes Rd, Werribee, Vic. 3030, Australia.

D Corresponding author. Email: rjacob@agric.wa.gov.au

Australian Journal of Agricultural Research 58(4) 374-382 https://doi.org/10.1071/AR06126
Submitted: 21 April 2006  Accepted: 11 January 2007   Published: 12 April 2007

Abstract

The aim of this experiment was to compare the effects of electrical stimulation and lamb age on the retail colour stability of lamb meat. Poll Dorset Merino crossbred lambs that were 5 months (‘suckers’, SU) and 12 months (‘carryovers’, CO) finished on the same green annual pasture were slaughtered at a commercial abattoir. Half of the carcasses in each age group (10) were either electrically stimulated (ES) or not stimulated (NES) post-dressing with a commercial high voltage electrical stimulation system. Luminescence (L), hue angle (indication of redness), chroma (intensity), and oxy/met (a measure of browning) values were measured at 0 h (0 days), 21 h (0.86 days), 93 h (3.88 days), and 166 h (6.91 days) after meat had been cut 1 day after slaughter, over wrapped, and put under simulated retail display conditions. Three muscles, m. longissimus thoracis et lumborum (LL), m. gluteus medias (GM), and m. rectus femoris (RF), were measured in this way. A linear mixed model was used to fit a repeated-measurements model to the light measurements of L, hue angle, chroma, and oxy/met. Oxy/met rate of change was compared by fitting splines to predict the time required for oxy/met to reach an arbitrary benchmark value of 3.

Colour and rate of colour change during display were affected by both age class and electrical stimulation but these effects depended on the muscle. The LL was the darkest (lowest L) and reddest (lowest hue angle), whilst RF was the most stable in colour (lowest rate of change for oxy/met) of the 3 muscles tested. Age class had a greater effect on oxy/met rate of change for the LL and GM compared with RF. Oxy/met of LL changed more rapidly for CO than SU lambs. ES increased the time for oxy/met to reach 3 in RF and GM for SU only but did so for both age groups in LL. ES decreased this time in RF and GM for CO. CO meat contained a higher concentration of myoglobin than SU meat. Negative effects of electrical stimulation on colour stability are more likely to occur in older lambs and in the GM and RF rather than in the LL.

Additional keywords: metmyoglobin, oxymyoglobin, shelf life.


Acknowledgments

The authors are appreciative of the funding provided by Meat and Livestock Australia and for the combined resources of the Sheep Meat Eating Quality team from Agriculture WA, NSW Agriculture, DPI Victoria, University of New England, and Murdoch University in conducting this project.


References


Anon. (1998) ‘Handbook of Australian meat.’ (Ausmeat: Brisbane, Qld)

Bekhit AED, Faustman C (2005) Metmyoglobin reducing activity in fresh meat: a review. Meat Science 71, 407–439.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Bekhit AED, Geesink GH, Morton JD, Bickerstaffe R (2001) Metmyoglobin reducing activity and colour stabilty of ovine longisimuss muscle. Meat Science 57, 427–435.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Bekhit AED, Geesink GH, Ilian MA, Morton JD, Sedcote JR, Bickerstaffe R (2003) Particulate metmyoglobin reducing activity and its relationship with meat colour. Journal of Agricultural and Food Chemistry 51, 6026–6035.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Bieri JG, Pollard CJ, Prange I, Dam H (1961) The determination of α-tocopherol in animal tissues by column chromatography. Acta Chemica Scandinavica. Series A: Physical and Inorganic Chemistry 15, 783–790. open url image1

Bradford M (1976) Determination of protein concentration in the manufacture and characterization of biopharmaceuticals. Analytical Biochemistry 72, 248–254.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Brandstetter AM, Picard B, Geay Y (1997) Regional variations of muscle fibre characteristics in m. semitendinosus of growing cattle. Journal of Muscle Research and Cell Motility 18, 57–62.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Brandstetter AM, Picard B, Geay Y (1998) Muscle fibre characterisitics in four muscles of growing male cattle I. Postnatal differentiation. Livestock Production Science 53, 15–23.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Faustman C, Chan WKM, Schaefer DM, Havens A (1998) Beef color update: the role of vitamin E. Journal of Animal Science 76, 1019–1026.
| PubMed |
open url image1

Fry JM, Smith GM, McGrath MC, Speijers EJ, Allen JG (1993) Plasma and tissue concentrations of α-tocopherol during vitamin E depletion in sheep. The British Journal of Nutrition 69, 225–232.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Grunert KG, Bredahl L, Brunso K (2004) Consumer perception of meat quality and implications for product development in the meat sector— a review. Meat Science 66, 259–272.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Gutzke D, Trout GR (2002) Temperature and pH dependence of the autoxidation rate of bovine, ovine, porcine and cervine oxymyoglobin isolated from three different muscles—Longisimuss dorsi, Gluteus medias, and Biceps femoris. Journal of Agricultural and Food Chemistry 50, 2673–2678.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Gutzke DA , Trout GR , D’Arcy BR (1997) The relationship between the colour stability and the formation of the metmyoglobin layer at the surface of beef, pork and venison muscles. In ‘43rd International Congress of Meat Science and Technology’. Auckland, New Zealand. (Ed. J Bass) pp. 652–653. (The Congress: Auckland, New Zealand)

Hopkins DL (1996) Assessment of lamb meat colour. Meat Focus International 5, 400–401. open url image1

Hunt MC , Acton J , Benedict R , Calkins C , Cornforth D , Jeremiah L , Olsen D , Salm C , Savell J , Shivas S (1991) ‘Guidelines for meat color evaluation.’ pp. 1–17. (American Meat Science Association, Kansas State University: Manhattan, KS)

Jose C (2004) Colour stability of sheep meat. Honours thesis, Murdoch University, Western Australia.

Kropf DH (1993) Colour stability—factors affecting the colour stability of fresh meat. Meat Focus International 2, 269–275. open url image1

Lynch A, Kerry JP, O’Sullivan MG, Lawlor JBP, Buckley DJ, Morrissey PA (2000) Distribution of α-tocopherol in beef muscles following dietary α-tocopherol acetate supplementation. Meat Science 56, 211–214.
| Crossref | GoogleScholarGoogle Scholar | open url image1

McMurray CH, Blanchflower WJ (1979) Application of high performance liquid chromatographic fluorescence method for the rapid determination of α tocopherol in the plasma of cattle and pigs and its comparison with direct fluorescence and high performance liquid chromatography—ultraviolet detection methods. Journal of Chromatography 178, 525–531.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Menzies DJ , Hopkins DL (1996) Relationship between colour and pH in lamb loins. In ‘Proceedings of the Australian Society of Animal Production’. Brisbane, Qld. (Ed. J Walker) p. 353. (University of Queensland: Brisbane, Qld)

Mitsumoto M, Ozawa S, Mitsuhashi T, Keide K (1998) Effect of dietary vitamin E supplementation for one week before slaughter on drip colour and lipid stability during display in Japanese Black Steer beef. Meat Science 49, 165–174.
| Crossref |
open url image1

Moore VJ, Young OA (1991) The effects of electrical stimulation, thawing, ageing and packaging on the colour and display life of lamb chops. Meat Science 30, 131–145.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Payne RW (2005) Modelling repeated measurements data. Ch. 5.4. In ‘The guide to GenStat® Release 8. Part 2: Statistics’. (VSN International: Hemel Hempstead, UK)

Pethick DW, Hopkins DL, D’Souza DN, Thompson JM, Walker PJ (2005) Effects of animal age on the eating quality of sheep meat. Australian Journal of Experimental Agriculture 45, 491–498.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Russell B, McAllister G, Ross IS, Pethick DW (2005) Lamb and sheep meat eating quality—industry and scientific issues and the need for integrated research. Australian Journal of Experimental Agriculture 45, 465–467.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Salviati G, Betto R, Margeth A, Novello F, Bonetti E (1980) Differential binding of vitamin E to sarcoplasmic reticulum from fast and slow muscles of the rabbit. Experientia 36, 1140–1141.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Trout GR (1991) A rapid method for measuring pigment concentration in porcine and other low pigmented muscles. In ‘37th International Congress of Meat Science and Technology’. Kulmbach, Germany. (Ed. J Horwitz) pp. 1198–1201. (The Congress: Kulmbach, Germany)

Trout GR (2003) Biochemistry of lipid and myoglobin oxidation in postmortem muscle and processed meat products—effects on rancidity. In ‘49th International Congress of Meat Science and Technology’. Campinas, Brazil. (Ed. N Beraquet) pp. 50–55. (Brazilian Journal of Food Technology: Campinas, Brazil)

Verbyla AP, Cullis BR, Kenward MG, Welham SJ (1999) The analysis of designed experiments and longitudinal data by using smoothing splines. Applied Statistics 48, 269–311. open url image1

Welham S, Cullis B, Gogel B, Gilmour A, Thompson R (2004) Prediction in linear mixed models. Australian and New Zealand Journal of Statistics 46, 325–347.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Wulf DM, Morgan JB, Sanders SK, Tatum JD, Smith GC, Williams S (1995) Effects of dietary supplementation of vitamin E on storage and caselife properites of lamb retail cuts. Journal of Animal Science 73, 399–405.
| PubMed |
open url image1

Zhang C, Jiang J, Zhang Y, Jia C (2005) Interaction of dietary iron and vitamin A influences the performance of broilers. Australian Journal of Agricultural Research 56, 435–442.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Zhu LG, Brewer MS (1998) Discolouration of fresh pork as related to muscle and display conditions. Journal of Food Science 63, 763–767.
| Crossref | GoogleScholarGoogle Scholar | open url image1