The high molecular weight glutenin subunit composition in old and modern bread wheats cultivated in Iran
Ali-Akbar Shahnejat-Bushehri A C , Masoud Gomarian B and Bahman Yazdi-Samadi AA Faculty of Agriculture, University of Tehran, Karaj, Iran.
B Islamic Azad University, Science and Research Branch, Tehran, Iran.
C Corresponding author. Email: ashah@ut.ac.ir
All authors contributed equally to this article.
Australian Journal of Agricultural Research 57(10) 1109-1114 https://doi.org/10.1071/AR06015
Submitted: 11 August 2005 Accepted: 22 May 2006 Published: 27 September 2006
Abstract
All current and old wheat cultivars grown in Iran were characterised by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE). The high-molecular-weight glutenin subunit (HMW-GS) banding patterns for each cultivar were assigned a Glu-1 quality score, a theoretical quality score based on Payne’s Glu-1 quality assignments. At the Glu-A1 loci, HMW-GS subunit compositions N, 7 + 8, 2 + 12 and 2*, 7 + 8, 2 + 12 were found to be predominant being expressed in 24 and 15 cultivars, respectively, out of 95. Eighteen different alleles were identified for the 3 loci studied: Glu-A1 (3), Glu-B1 (9), and Glu-D1 (6). The glutenin quality scores of Iranian wheat ranged from 4 to 10, with an average of 7.4. It was found that some cultivars were heterogeneous in HMW-GS composition. In cv. Cooleh, only one glutenin subunit at the Glu-B1 locus was present. HMW-GS 2*** + 12′ was found in 6 cultivars and biotypes. The results obtained here describing the allelic composition of bread wheat commonly grown in Iran may be useful in wheat breeding programs selecting for good quality parameters.
Additional keywords: Iranian bread wheat, HMW-GS, bread-making quality.
Acknowledgments
This research was carried out as part of a loss reduction in wheat production and consumption project, funded by the Iranian Scientific Research Council (Agriculture Commission), National Research Program. The authors gratefully acknowledge this support.
Bahraei S,
Saidi A, Alizadeh D
(2004) High molecular weight glutenin subunits of current bread wheats grown in Iran. Euphytica 137, 173–179.
| Crossref | GoogleScholarGoogle Scholar |
Bietz JA,
Shepherd KW, Wall JC
(1975) Single kernel analysis of glutenin: use in wheat genetics and breeding. Cereal Chemistry 52, 513–532.
Branlard G,
Dardevet M,
Amiour N, Igrejas G
(2003) Allelic diversity of HMW and LMW glutenin subunits and omega-gliadins in French bread wheat (Triticum aestivum L.). Genetic Resources and Crop Evolution 50, 669–679.
| Crossref | GoogleScholarGoogle Scholar |
Cornish CB,
Bekes F,
Allen HM, Martin DJ
(2001) Flour proteins linked to quality traits in an Australian doubled haploid wheat population. Australian Journal of Agricultural Research 52, 1339–1348.
| Crossref | GoogleScholarGoogle Scholar |
Dong H,
Cox TS,
Sears RC, Lookhart GL
(1991) High molecular weight glutenin genes: effect on quality in wheat. Crop Science 31, 974–979.
Gianibelli MC,
Larroque OR,
MacRitchie F, Wrigley CW
(2001) Biochemical, genetic, and molecular characterization of wheat glutenin and its component subunits. Cereal Chemistry 78, 635–646.
Gregov E,
Tisov V, Kraic J
(1997) Genetic variability at the Glu-1 loci in old and modern wheats (Triticum aestivum L.) cultivated in Slovakia. Genetic Resources and Crop Evolution 44, 301–306.
| Crossref | GoogleScholarGoogle Scholar |
Gupta RB,
Paul IG,
Cornish GB,
Palmer GA,
Bekes F, Rathjen AJ
(1994) Allelic variation at glutenin subunit and gliadin loci, Glu-1, Glu-3 and Gli-1, of common wheats. Its additive and interaction effects of dough properties. Journal of Cereal Science 19, 9–17.
| Crossref | GoogleScholarGoogle Scholar |
Harberd NP,
Bartels D, Thompson RD
(1986) DNA restriction fragment variation in the gene family encoding high molecular weight (HMW) glutenin subunits of wheat. Biochemical Genetics 24, 579–596.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
He ZH,
Pena RJ, Rajaram S
(1992) High molecular weight glutenin subunit composition of Chinese bread wheat. Euphytica 64, 11–20.
Igrejas G,
Guedes-Pinto H,
Carnide V, Brandlard G
(1999) The high and low molecular weight subunits and ω-gliadins composition of bread and durum wheats commonly grown in Portugal. Plant Breeding 118, 297–302.
| Crossref | GoogleScholarGoogle Scholar |
Laemmli VK
(1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Lawrence GJ
(1986) The high-molecular-weight glutenin subunit composition of Australian wheat cultivars. Australian Journal of Agricultural Research 46, 451–460.
Lawrence GJ,
Moss HJ,
Shepherd KW, Wrigley CW
(1987) Dough quality of biotypes of eleven Ausralian wheat cultivars that differ in high-molecular-weight glutenin subunit composition. Journal of Cereal Science 6, 99–101.
Lookhart GL,
Hagman K, Kasarda DD
(1993) High-molecular-weight glutenin subunits of the most commonly grown wheat cultivars in the U.S. in 1984. Plant Breeding 110, 48–62.
| Crossref | GoogleScholarGoogle Scholar |
Lukow OM,
Payne PI, Tkachuk R
(1989) The HMW glutenin composition of Canadian wheat cultivars and their association with bread-making quality. Journal of the Science of Food and Agriculture 46, 451–460.
MacRitchie F
(1992) Physicochemical properties of wheat proteins in relation to functionality. Advances in Food and Nutrition Research 36, 1–87.
Margiotta B,
Colaprico G,
D’Ovido R, Lafiandra D
(1993) Characterization of high Mr subunits of glutenin by combined chromatographic (RP-HPLC) and electrophoretic separations and restriction fragment length polymorphism (RFLP) analysis of their encoding genes. Journal of Cereal Science 17, 221–236.
| Crossref | GoogleScholarGoogle Scholar |
McIntosh RA,
Hart GE, Gale MD
(1994) Catalogue of gene symbols for wheat. Annual Wheat Genetics Newsletter 40, 362–375.
Metakovsky EV
(1991) Gliadin allele identification in common wheat. II. Catalogue of gliadin alleles in common wheat. Journal of Genetics and Breeding 45, 325–344.
Morgunov AI,
Rogers WJ,
Sayers EJ, Metakovky EV
(1990) The high-molecular-weight glutenin subunit composition of Soviet wheat varieties. Euphytica 51, 41–52.
| Crossref | GoogleScholarGoogle Scholar |
Nakamura H
(2000) The association between high-molecular-weight glutenin subunit composition and bread-making quality of Chinese and Japanese hexaploid wheat. Australian Journal of Agricultural Research 51, 371–375.
| Crossref | GoogleScholarGoogle Scholar |
Nakamura H,
Inazu A, Hirano H
(1999) Allelic variation in high-molecular-weight glutenin subunit loci of Glu-1 in Japanese common wheats. Euphytica 106, 131–138.
| Crossref | GoogleScholarGoogle Scholar |
Nei M
(1973) Analysis of gene diversity in subdivided population. Proceedings of the National Academy of Sciences of the USA 70, 3321–3323.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Ng PKW,
Pogna NE,
Mellini F, Bushuk W
(1989) Glu-1 allele compositions of the wheat cultivars registered in Canada. Journal of Genetics and Breeding 43, 53–60.
Osborne TB
(1907) The proteins of the wheat kernel. Carnegie Institute, Washington DC Publication 84, 1–19.
Payne PI
(1987) Genetics of wheat storage proteins and the effect of allelic variation on bread-making quality. Annual Review of Plant Physiology 38, 141–153.
| Crossref | GoogleScholarGoogle Scholar |
Payne PI, Corfield KG
(1979) Subunit composition of wheat glutenin protein isolated by gel filtration in a dissociating medium. Planta 145, 83–88.
| Crossref | GoogleScholarGoogle Scholar |
Payne PI,
Holt LM,
Jackson EA, Law CN
(1984) Wheat storage proteins: their genetics, and their potential for manipulation by plant breeding. Philosophical Transactions of the Royal Society of London B304, 359–371.
Payne PI,
Holt LM,
Krattiger AF, Carrillo JM
(1988) Relationships between seed quality characteristics and HMW glutenin subunit composition determined using wheats grown in Spain. Journal of Cereal Science 7, 229–235.
Payne PI, Lawrence GJ
(1983) Catalogue of alleles for the complex gene loci, Glu-A1, Glu-B1 and Glu-D1 which code for high molecular-weight subunits of glutenin in hexaploid wheat. Cereal Research Communications 11, 29–35.
Payne PI,
Nightingale MA,
Krattiger AF, Holt LM
(1987) The relationship between HMW glutenin subunit composition and the bread-making quality of British-grown wheat varieties. Journal of The Science of Food and Agriculture 40, 51–65.
Pogna NE,
Mellini F,
Beretta A, Dal Belin Peruffo A
(1989) The high-molecular weight glutenin subunits of common wheat cultivars grown in Italy. Journal of Genetics and Breeding 43, 17–24.
Redaelli R,
Ng PKW, Pogna NE
(1997) Allelic variation at the storage protein loci of 55 US-grown white wheats. Plant Breeding 116, 429–436.
| Crossref | GoogleScholarGoogle Scholar |
Rogers WJ,
Payne PI, Harinder K
(1989) The HMW glutenin subunit and gliadin composition of German-grown wheat varieties and their relationship with bread-making quality. Plant Breeding 103, 89–100.
| Crossref | GoogleScholarGoogle Scholar |
Sontag T,
Salovaara H, Payne PI
(1986) The high-molecular-weight glutenin subunit compositions of wheat varieties bred in Finland. Journal of Agricultural Science 58, 151–156.
Tahir M,
Hussain SA,
Turchetta T, Lafiandra D
(1995) The HMW glutenin subunits of bread wheat varieties bred in Pakistan. Plant Breeding 114, 442–444.
| Crossref | GoogleScholarGoogle Scholar |
Uhlen AK
(1990) The composition of high molecular weight glutenin subunits in Norwegian wheats, and their relation to bread-making quality. Norwegian Journal of Agricultural Sciences 4, 1–17.
