RAMP based fingerprinting and assessment of relationships among Australian narrow-leafed lupin (Lupinus angustifolius L.) cultivars
H. Yuan A , G. Yan B E , K. H. M. Siddique C and H. Yang C DA Department of Biotechnology, Zhanjiang Ocean University, Guangdong, Zhanjiang, 524088, China.
B School of Plant Biology, Faculty of Natural and Agricultural Sciences, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.
C Centre for Legumes in Mediterranean Agriculture, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.
D Department of Agriculture Western Australia, 3 Baron-Hay Court, South Perth, WA 6151, Australia.
E Corresponding author. Email: gyan@plants.uwa.edu.au
Australian Journal of Agricultural Research 56(12) 1339-1346 https://doi.org/10.1071/AR05188
Submitted: 30 May 2005 Accepted: 12 August 2005 Published: 15 December 2005
Abstract
Narrow-leafed lupin is a major winter grain legume crop in the Australian farming system and a number of commercial cultivars are currently available to growers. A significant level of polymorphism was detected in narrow-leafed lupin cultivars by the randomly amplified microsatellite polymorphism (RAMP) approach, suggesting that cultivars harbour considerable DNA variation. Seventy-seven cultivar-specific markers were found among the 23 lupin cultivars examined and a dichotomous fingerprinting key was developed for the molecular identification of lupin cultivars. Cluster analysis of pairwise distance matrix computed from RAMP profiles grouped the 23 cultivars into 4–5 clusters, which generally agreed with their pedigree relationships.
Additional keywords: RAMP, lupin cultivars, dichotomous molecular identification key.
Acknowledgments
H. Yuan acknowledges the financial support by CLIMA and the School of Plant Biology during her visit to Western Australia in 2004/2005.
Astarini IA,
Plummer JA,
Lancaster RA, Yan G
(2004) Fingerprinting of cauliflower cultivars using RAPD markers. Australian Journal of Agricultural Research 55, 117–124.
| Crossref | GoogleScholarGoogle Scholar |
Chin ECL,
Senior ML, Smith JSC
(1996) Maize simple repetitive DNA sequences: abundance and allele variation. Genome 39, 866–873.
| PubMed |
Cowling WA, Huyghe C, Swiecicki W
(1998) Lupin breeding. ‘Lupins as crop plants: biology, production and utilization’. (Eds JS Gladstones, CA Atkins, J Hamblin)
pp. 93–120. (CAB International: London)
Davila JA,
Loarce Y, Ferrer E
(1999) Molecular characterization and genetic mapping of random amplified microsatellite polymorphism in barley. Theoretical and Applied Genetics 98, 265–273.
| Crossref | GoogleScholarGoogle Scholar |
Gladstones JS
(1994) A historical view of lupins in Australia. ‘Proceedings of the First Australian Lupin Technical Symposium’. (Ed. M Dracup ,
J Palta )
pp. 1–38. (Department of Agriculture Western Australia: Perth, W. Aust.)
Pharmawati M,
Yan G, Finnegan PM
(2005) Molecular variation and fingerprinting of Leucadendron cultivars (Proteaceae) by ISSR markers. Annals of Botany 95, 1163–1170.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Pradhan A,
Yan G, Plummer JA
(2004) Development of DNA fingerprinting keys for the identification of radish cultivars. Australian Journal of Experimental Agriculture 44, 95–102.
| Crossref | GoogleScholarGoogle Scholar |
Raeder U, Broda P
(1985) Rapid preparation of DNA from filamentous fungi. Letters in Applied Microbiology 1, 17–20.
Shan F,
Clarke H,
Yan G,
Plummer JA, Siddique KHM
(2004) Development of DNA fingerprinting keys for discrimination of Cicer echinospermum (P.H. Davis) accessions using AFLP markers. Australian Journal of Agricultural Research 55, 947–952.
| Crossref | GoogleScholarGoogle Scholar |
Siddique KHM, Sykes J
(1997) Pulse production in Australia past, present and future. Australian Journal of Experimental Agriculture 37, 103–111.
| Crossref | GoogleScholarGoogle Scholar |
Swofford DL
(1998)
Taramino G, Tingey S
(1996) Simple sequence repeats for germplasm analysis and mapping in maize. Genome 39, 277–287.
| PubMed |
Wu K,
Jones R,
Danneberger L, Scolnik PA
(1994) Detection of microsatellite polymorphisms without cloning. Nucleic Acids Research 22, 3257–3258.
| PubMed |
Yang H,
Boersma JG,
You M,
Buirchell BJ, Sweetingham MW
(2004) Development and implementation of a sequence-specific PCR marker linked to a gene conferring resistance to anthracnose disease in narrow-leafed lupin (Lupinus angustifolius L.). Molecular Breeding 14, 145–151.
| Crossref | GoogleScholarGoogle Scholar |
Yang H,
Shankar M,
Buirchell BJ,
Sweetingham MW,
Caminero C, Smith PMC
(2002) Development of molecular markers using MFLP linked to a gene conferring resistance to Diaporthe toxica in narrow-leafed lupin (Lupinus angustifolius L.). Theoretical and Applied Genetics 105, 265–270.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Yang H,
Sweetingham MW,
Cowling WA, Smith PMC
(2001) DNA fingerprinting based on microsatellite-anchored fragment length polymorphisms, and isolation of sequence-specific PCR markers in lupin (Lupinus angustifolius L.). Molecular Breeding 7, 203–209.
| Crossref | GoogleScholarGoogle Scholar |
You M,
Boersma JG,
Buirchell BJ,
Sweetingham MW,
Siddique KHM, Yang H
(2005) A PCR-based molecular marker applicable for marker-assisted selection for anthracnose disease resistance in lupin breeding. Cellular and Molecular Biology Letters 10, 123–134.
| PubMed |
Zietkiewicz E,
Rafalski A, Labuda D
(1994) Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics 20, 176–183.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
