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RESEARCH ARTICLE
Contents Vol 56(4)

A genetic linkage map in autotetraploid lucerne adapted to northern Australia, and use of the map to identify DNA markers linked to resistance to Phytophthora medicaginis

J. M. Musial A , K. S. Aitken B , J. M. Mackie A and J. A. G. Irwin A C
+ Author Affiliations
- Author Affiliations

A Cooperative Research Centre for Tropical Plant Protection, The University of Queensland, Brisbane, Qld 4072, Australia.

B CSIRO Plant Industry, Queensland Bioscience Precinct, St Lucia, Qld 4067, Australia.

C Corresponding author. Email: j.irwin@tpp.uq.edu.au

Australian Journal of Agricultural Research 56(4) 333-344 https://doi.org/10.1071/AR04317
Submitted: 20 December 2004  Accepted: 1 March 2005   Published: 26 April 2005

Abstract

Phytophthora root rot, caused by Phytophthora medicaginis, is a major limitation to lucerne (Medicago sativa L.) production in Australia and North America. Quantitative trait loci (QTLs) involved in resistance to P. medicaginis were identified in a lucerne backcross population of 120 individuals. A genetic linkage map was constructed for tetraploid lucerne using 50 RAPD (randomly amplified polymorphic DNA), 104 AFLP (amplified fragment length polymorphism) markers, and one SSR (simple sequence repeat or microsatellite) marker, which originated from the resistant parent (W116); 13 markers remain unlinked. The linkage map contains 18 linkage groups covering 2136.5 cM, with an average distance of 15.0 cM between markers. Four of the linkage groups contained only either 2 or 3 markers. Using duplex markers and repulsion phase linkages the map condensed to 7 homology groups and 2 unassigned linkage groups. Three regions located on linkage groups 2, 14, and 18, were identified as associated with root reaction and the QTLs explained 6–15% of the phenotypic variation. The research also indicates that different resistance QTLs are involved in conferring resistance in different organs. Two QTLs were identified as associated with disease resistance expressed after inoculation of detached leaves. The marker, W11-2 on group 18, identified as associated with root reaction, contributed 7% of the phenotypic variation in leaf response in our population. This marker appears to be linked to a QTL encoding a resistance factor contributing to both root and leaf reaction. One other QTL, not identified as associated with root reaction, was positioned on group 1 and contributed to 6% of the variation. This genetic linkage map provides an entry point for future molecular-based improvement of lucerne in Australia, and markers linked to the QTLs we have reported should be useful for marker-assisted selection for partial resistance to P. medicaginis in lucerne.

Additional keywords: alfalfa, SDRF, disease resistance, molecular markers, QTL mapping.


Acknowledgments

The authors thank the CRC for Tropical Plant Protection and the GRDC (Grains Research and Development Corporation) for providing funding support for the project. We also thank Dr C. L. McIntyre, Prof. E. T. Bingham, Prof. T. C. Osborn, Prof. M. J. Havey, and Dr B. Julier for discussions on autotetraploid genetics.


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