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RESEARCH ARTICLE
Contents Vol 55(12)

Identification of microsatellite markers associated with the cereal cyst nematode resistance gene Cre3 in wheat

E. M. Martin A C D , R. F. Eastwood B C and F. C. Ogbonnaya A C
+ Author Affiliations
- Author Affiliations

A Department of Primary Industries (Primary Industries Research Victoria), Private Bag 260, Horsham, Vic. 3401, Australia.

B Australian Grain Technologies, Private Bag 260, Horsham, Vic. 3401, Australia.

C Molecular Plant Breeding Cooperative Research Centre, Suite 21, 2 Park Drive, Bundoora, Vic. 3083, Australia.

D Corresponding author. Email: erica.martin@dpi.vic.gov.au

Australian Journal of Agricultural Research 55(12) 1205-1211 https://doi.org/10.1071/AR04085
Submitted: 13 April 2004  Accepted: 22 October 2004   Published: 21 December 2004

Abstract

Cereal cyst nematode (CCN) is a root disease caused by the pathogen Heterodera avenae Woll. that significantly reduces wheat (Triticum aestivum L.) grain yields in temperate countries. The Cre3 gene, located on chromosome 2DL, provides high levels of resistance to the Australian pathotype and isolates from Syria and Algeria, and has become available to wheat breeders. Selection for lines carrying the Cre3 gene in Australian wheat breeding programs is currently based on a dominant PCR marker (Cre3spf/r) diagnostic for the Cre3 gene. However, this marker has limitations that increase the cost and reduce selection efficiency in screening early-generation breeding lines. Such limitations would be minimised by the identification of a microsatellite marker linked to the Cre3 gene. We have constructed 2 genetic linkage maps of wheat chromosome 2DL and identified microsatellite markers mapping closely to the diagnostic Cre3spf/r marker. These closely linked markers were validated in a diverse range of germplasm, and one microsatellite marker, Xgwm301, which mapped 4 cM from Cre3spf/r, was shown to be highly associated with the presence of the Cre3 gene. Amplification conditions for the Xgwm301 locus were optimised, and its use in marker-assisted selection to identify Cre3 CCN-resistant wheat in the Australian Grain Technologies breeding program is demonstrated.

Additional keywords: Aegilops tauschii, MAS, molecular marker, pyramiding, SSR.


Acknowledgments

The authors would like to acknowledge the financial support of the Grains Research and Development Cooperation and the support of the CRC for Molecular Plant Breeding. They would like to thank Dr Howard Eagles for his valuable comments on the manuscript, Dr Livinus Emebiri for his assistance with the mapping work and Jayne Wilson for her contributions with the biological assay.


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