Register      Login
Crop and Pasture Science Crop and Pasture Science Society
Plant sciences, sustainable farming systems and food quality
Shopping Cart: ( empty )
You are here: Home > Journals > CP > AR04082
RESEARCH ARTICLE
Contents Vol 56(7)

The role of osmotolerant rhizobacteria in rice growth under different drought conditions

Triwibowo Yuwono A B , Desi Handayani A and Joedoro Soedarsono A
+ Author Affiliations
- Author Affiliations

A Laboratory of Microbiology, Faculty of Agriculture, Gadjah Mada University, Bulaksumur, Yogyakarta 55281, Indonesia.

B Corresponding author. Email: triwibowo@faperta.ugm.ac.id

Australian Journal of Agricultural Research 56(7) 715-721 https://doi.org/10.1071/AR04082
Submitted: 5 April 2004  Accepted: 2 May 2005   Published: 22 July 2005

Abstract

Osmotolerant rhizobacterial isolates were used as inoculants for rice under different drought conditions in a greenhouse. Rice was grown on a Regosol, under either sterile or non-sterile conditions, in which the water level was adjusted to 80% and 40% of field capacity. Rice seed was inoculated with osmotolerant rhizobacterial isolates (isolates Al-19, A82, and M7b) and grown until the plant reached maximum vegetative phase (86 days from seed sowing). Inocula used were: Al-19 plus A82, Al-19 plus M7b, A82 plus M7b, and a mixture of 3 isolates.

Under sterile conditions, inoculation of rice with the different inocula resulted in an increase in shoot dry weight, root dry weight, and number of tillers of 0–45.72%, 0.62–79.67%, and 0– 61.11%, respectively. Under non-sterile condition, shoot dry weight, root dry weight, and number of tillers increased by 19.66–28.04%, 14.00–91.33%, and 14.29–157.14%, respectively. The study also demonstrated that the role of osmotolerant rhizobacteria was more pronounced at 40% field capacity than 80% field capacity.

Additional keywords: osmotolerant rhizobacteria, drought stress, rice.


Acknowledgment

This work was part of a research project funded by the Directorate General of Higher Education, Ministry of Education and Culture, Republic of Indonesia, through the Graduate Team Research Grant Project (URGE Project) awarded to Joedoro Soedarsono and Triwibowo Yuwono.


References


Anon., (1994). Soil taxonomy. (United States Department of Agriculture (USDA)

Csonka LN (1989) A third L-proline permease in Salmonella typhimurium which functions in media of elevated osmotic strength. Journal of Bacteriology 151, 1433–1443. open url image1

De Datta, SK (1975). ‘Major research in upland rice.’ (IRRI: Los Banos, Philippines)

Galinski EA, Truper HG (1994) Microbial behaviour in salt-stressed ecosystems. FEMS Microbiology Review 15, 95–108.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Landfald B, Strom AR (1986) Choline-glycine pathway confers a high level of osmotic tolerance in Escherichia coli.  Journal of Bacteriology 165, 849–855.
| PubMed |
open url image1

Marthi B, Lighthart B (1990) Effects of betaine on enumeration of airborne bacteria. Applied and Environmental Microbiology 56, 1286–1289.
| PubMed |
open url image1

Hutkins RW, Ellefson WL, Kashket ER (1987) Betaine transport imparts osmotolerance on a strain of Lactobacillus acidophilus.  Applied and Environmental Microbiology 53, 2275–2281. open url image1

Peng S, Biswas JC, Ladha K, Gyaneshwar P, Chen Y (2002) Influence of rhizobial inoculation on photosynthesis and grain yield of rice. Agronomy Journal 94, 925–929. open url image1

Rudulier DL, Bouillard L (1983) Glycine betaine, an osmotic effector in Klebsiella pneumoniae and other members of the Enterobacteriaceae.  Applied and Environmental Microbiology 46, 152–159.
| PubMed |
open url image1

Smith LT, Pocard J-A, Bernard T, Rudulier DL (1988) Osmotic control of glycine betaine biosynthesis and degradation in Rhizobium meliloti.  Journal of Bacteriology 170, 3124–3149. open url image1

Tien TM, Gaskins MH, Hubbell DH (1979) Plant growth substances produced by Azospirillum brasilense and their effect on the growth of pearl millet (Penisettum americanum L.). Applied and Environmental Microbiology 37, 1016–1024. open url image1

Turner, GJ ,  and  Gibson, AH (1980). Measurement of nitrogen fixation by indirect means. In ‘Method for evaluation of biological nitrogen fixation.’ (John Wiley and Sons, Inc.: New York)

Yuwono T, Susilowati LE, Ikhwan A, Ngadiman , Shovitri M, Mursyanti E, Soedarsono J (1997) The potency of drought-tolerant rhizobacteria as inoculants for Gogo rice. In ‘Proceedings of IC Biotech’. (Osaka University: Osaka, Japan)


Yuwono T, Mursyanti E, Soedarsono J (1999) Cloning of betain aldehyde dehydrogenase gene of Escherichia coli and its use as a probe for related gene in salt-tolerant rhizobacteria. Indonesian Journal of Biotechnology June (1999) , open url image1