Seasonal changes in polyamine content of vegetative and reproductive olive organs in relation to floral initiation, anthesis, and fruit development
T. S. Pritsa A and D. G. Voyiatzis A BA Aristotle University of Thessaloniki, Department of Horticulture, Lab. of Biology of Horticultural Plants, 541 24 Thessaloniki, Greece.
B Corresponding author; email: voyiatzi@agro.auth.gr
Australian Journal of Agricultural Research 55(10) 1039-1046 https://doi.org/10.1071/AR04056
Submitted: 9 March 2004 Accepted: 11 August 2004 Published: 25 October 2004
Abstract
The concentrations of free and non-free spermidine and spermine in vegetative (leaves, shoot apices) and reproductive (lateral buds, ovaries, fruit tissues) olive organs were determined. The aim was to investigate whether the seasonal fluctuations of these polyamines in different organs correlated to developmental processes such as floral differentiation, shoot growth, anthesis, fertilisation, and fruit growth. Samples were taken from trees of the large-fruited cultivar Chondrolia Chalkidikis and the small-fruited Koroneiki. High levels of both polyamines were determined in buds and shoot apices during the cold period, especially in Ch. Chalkidikis, possibly connected to cold-hardiness exhibited by this cultivar. Two peaks of total spermine in buds, the first occurring in January–February and the second in mid April, coincided with the morphological differentiation of flowers and the inflorescence emergence, respectively. Polyamine levels in ovaries dropped after ovule fertilisation and remained low during the subsequent growth of fruit tissues. Spermidine accumulation, especially in the non-free form, was recorded around the middle of August during a period of intense physiological activity in olive connected to embryo and fruit growth, endocarp hardening, and preceding the second flush of growth in early September. The data showed a direct relationship, in terms of time, of polyamine fluctuations with developmental processes in olive. Whether polyamines act, in this respect, as nitrogen providers or assume a more active regulatory role remains to be elucidated.
Additional keywords: Olea europaea L., growth regulators, flowering, fruit set.
Acknowledgments
Dr Theodora Pritsa is grateful to the National Scholarship Foundation for financial support during her doctorate research, part of which is presented in this paper.
Aziz A
(2003) Spermidine and related-metabolic inhibitors modulate sugar and amino acid levels in Vitis vinifera L.: possible relationships with initial fruitlet abscission. Journal of Experimental Botany 54, 355–363.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Badr SA, Hartmann HT
(1971) Effect of diurnally fluctuating vs. constant temperatures on flower induction and sex expression in the olive (Olea europaea). Physiologia Plantarum 24, 40–45.
Bagni N, Altamura MM, Biondi S, Mengoli M, Torrigiani P
(1993) Polyamines and morphogenesis in normal and transgenic plant cultures. ‘Morphogenesis in plants’. (Eds KA Roubelakis-Angelakis, Van K Tran Thanh)
pp. 89–111. (Plenum Press: New York, USA)
Bartolozzi F,
Rocchi P,
Camerini F, Fontanazza G
(1999) Changes of biochemical parameters in olive (Olea europaea L.) leaves during an entire vegetative season, and their correlation with frost resistance. Acta Horticulturae 474, 435–440.
Biasi R,
Bagni N, Costa G
(1988) Endogenous polyamines in apple and their relationship to fruit set and fruit growth. Physiologia Plantarum 73, 205–210.
Bouchereau A,
Aziz A,
Larher F, Martin-Tanguy J
(1999) Polyamines and environmental challenges: recent development. Plant Science 140, 103–125.
| Crossref | GoogleScholarGoogle Scholar |
Caffaro SV, Vicente C
(1995) Early changes in the content of leaf polyamines during the photoperiodic flowering induction in soybean. Journal of Plant Physiology 145, 756–758.
Drossopoulos JB, Niavis CA
(1988) Seasonal changes of the metabolites in leaves, bark and xylem tissues of olive tree (Olea europaea L.) II. Carbohydrates. Annals of Botany 62, 321–327.
Evans PT, Malmberg R
(1989) Do polyamines have a role in plant development? Annual Reviews of Plant Physiology and Plant Molecular Biology 40, 235–269.
Faust M, Wang AY
(1992) Polyamines in horticulturally important plants. Horticultural Reviews 14, 333–356.
Fernández-Escobar R,
Benlloch M,
Navarro C, Martin GC
(1992) The time of floral induction in the olive. Journal of the American Society for Horticultural Science 117, 304–307.
Flores HE, Galston AW
(1982) Analysis of polyamines in higher plants by high performance liquid chromatography. Plant Physiology 69, 701–706.
Galston AW, Kaur-Sawhney R
(1995) Polyamines as endogenous growth regulators. ‘Plant hormones: physiology, biochemistry and molecular biology’. (Ed. PJ Davies)
pp. 158–178. (Kluwer Academic Publishers: Dordrecht, The Netherlands)
Guye MG,
Vigh L, Wilson JM
(1986) Polyamine titer in relation to chill-sensitivity in Phaseolus sp. Journal of Experimental Botany 37, 1036–1043.
Hackett WP, Hartmann HT
(1967) The influence of temperature on floral initiation in the olive. Physiologia Plantarum 20, 430–436.
Kakkar RK, Rai VK
(1993) Plant polyamines in flowering and fruit ripening. Phytochemistry 33, 1281–1288.
| Crossref | GoogleScholarGoogle Scholar |
Kakkar RK, Sawhney VK
(2002) Polyamine research in plants—a changing perspective. Physiologia Plantarum 116, 281–292.
| Crossref | GoogleScholarGoogle Scholar |
Kaur-Sawhney R, Applewhite PB
(1993) Endogenous protein-bound polyamines: correlation with regions of cell division in tobacco leaves, internodes and ovaries. Plant Growth Regulation 12, 223–227.
| Crossref |
Königshofer H
(1989) Seasonal changes in polyamine content in different parts of juvenile spruce trees [Picea abies (L.) Karst.]. Journal of Plant Physiology 134, 736–740.
Königshofer H
(1990) Polyamine content in needles, shoot-axes, buds and xylem exudates of mature spruce trees [Picea abies (L.) Karst.]. Journal of Plant Physiology 136, 377–380.
Kotzabasis K,
Christakis-Hampsas MD, Roubelakis-Angelakis KA
(1993) A narrow-bore HPLC method for the identification and quantitation of free, conjugated and bound polyamines. Analytical Biochemistry 214, 484–489.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Kushad MM
(1998) Changes in polyamine levels in relationship to the double-sigmoidal growth curve of peaches. Journal of the American Society for Horticultural Science 123, 950–955.
Lavee S,
Harshemesh H, Avidan N
(1986) Phenolic acids—possible involvement in regulating growth and alternate fruiting in olive trees. Acta Horticulturae 179, 317–328.
| Crossref | GoogleScholarGoogle Scholar |
Manrique T,
Rapoport HF,
Castro J, Pastor M
(1999) Mesocarp cell division and expansion in the growth of olive fruit. Acta Horticulturae 474, 301–304.
Minocha R,
Smith DR,
Reeves C,
Steele KD, Minocha SC
(1999) Polyamine levels during the development of zygotic and somatic embryos of Pinus radiata.
Physiologia Plantarum 105, 155–164.
| Crossref | GoogleScholarGoogle Scholar |
Minocha SC,
Minocha R, Robie CA
(1990) High-performance liquid chromatographic method for the determination of dansyl-polyamines. Journal of Chromatography 511, 177–183.
| Crossref | GoogleScholarGoogle Scholar |
Navarro C,
Fernández-Escobar R, Benlloch M
(1990) Flower bud induction in ‘Manzanillo’ olive. Acta Horticulturae 286, 195–198.
Pannelli G,
Famiani F, Rugini E
(1992) Effects of change in ploidy level on anatomical, cytological, reproductive and growth performance changes and polyamine contents, in mutants of gamma irradiated olive plants. Acta Horticulturae 317, 209–215.
Rallo L, Martin GC
(1991) The role of chilling in releasing olive floral buds from dormancy. Journal of the American Society for Horticultural Science 116, 1058–1062.
Rallo L,
Torrepo P,
Vergas A, Alvarado J
(1994) Dormancy and alternate bearing in olive. Acta Horticulturae 356, 127–136.
Rey M,
Díaz-Sala C, Rodríguez R
(1994) Comparison of endogenous polyamine content in hazel leaves and buds between the annual dormancy and flowering phases of growth. Physiologia Plantarum 91, 45–50.
| Crossref |
Rugini E, Mencuccini M
(1985) Increased yield in the olive with putrescine treatment. HortScience 20, 102–103.
Sagee O, Lovatt CJ
(1991) Putrescine content parallels ammonia and arginine metabolism in developing flowers of the ‘Washington’ Navel orange. Journal of the American Society for Horticultural Science 116, 280–285.
Shen W,
Nada K, Tachibana S
(2000) Involvement of polyamines in the chilling tolerance of cucumber cultivars. Plant Physiology 124, 431–439.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Shiozaki S,
Ogata T, Horiuchi S
(2000) Endogenous polyamines in the pericarp and seed of the grape berry during development and ripening. Scientia Horticulturae 83, 33–41.
| Crossref | GoogleScholarGoogle Scholar |
Smith MA, Davies PJ
(1985) Separation of polyamines in plant tissue by high performance liquid chromatography of their dansyl derivatives. Plant Physiology 78, 89–91.
Tarenghi E, Martin-Tanguy J
(1995) Polyamines, floral induction and floral development of strawberry (Fragaria ananassa Duch.). Plant Growth Regulation 17, 157–165.
| Crossref |
Ulger S,
Sonmez S,
Karkacier M,
Ertoy N,
Akdesir O, Aksu M
(2004) Determination of endogenous hormones, sugar and mineral nutrition levels during the induction, initiation and differentiation stage and their effects on flower formation in olive. Plant Growth Regulation 42, 89–95.
| Crossref | GoogleScholarGoogle Scholar |
Wang SY, Faust M
(1993) Comparison of seasonal growth and polyamine content in shoot of orchard-grown standard and genetic dwarf apple trees. Physiologia Plantarum 89, 376–380.
| Crossref | GoogleScholarGoogle Scholar |
Wang SY, Faust M
(1994) Changes in polyamine content during dormancy in flower buds of ‘Anna’ apple. Journal of the American Society for Horticultural Science 119, 70–73.
