Potential of capillary electrophoresis for identification of Australian triticale varieties
S. Siriamornpun A , M. Wootton B C and J. B. Schultheiss BA Department of Food Technology and Nutrition, Faculty of Technology, Mahasarakham University, Mahasarakham 44000 Thailand.
B School of Chemical Engineering and Industrial Chemistry (Food Science and Technology), the University of New South Wales, Sydney, NSW 2052, Australia.
C Corresponding author; email: m.wootton@unsw.edu.au
Australian Journal of Agricultural Research 55(5) 595-598 https://doi.org/10.1071/AR03220
Submitted: 22 October 2003 Accepted: 26 February 2004 Published: 8 June 2004
Abstract
Ethanol soluble proteins were extracted from 5 different triticale varieties grown at 2 different sites in South Australia, and from single samples of wheat and rye. Separation of these proteins using capillary electrophoresis (CE) showed different patterns for different varieties but similar patterns for the same varieties grown at the 2 sites. The main differences between electropherograms for wheat and triticale were attributed to the presence of rye secalins, higher levels of α-gliadins, and lower levels of β-gliadins in those from the latter. These results indicate the potential of CE of ethanol-soluble proteins for identification of triticale varieties.
Additional keywords: triticale, ethanol-soluble proteins, varietal identification.
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