In vitro shoot regeneration from commercial cultivars of Australian canola (Brassica napus L.)
Yan Zhang A and Prem L Bhalla A BA Plant Molecular Biology and Biotechnology Laboratory, Institute of Land and Food Resources, The University of Melbourne, Parkville, Vic. 3010, Australia.
B Corresponding author; email: premlb@unimelb.edu.au
Australian Journal of Agricultural Research 55(7) 753-756 https://doi.org/10.1071/AR03209
Submitted: 15 October 2003 Accepted: 20 April 2004 Published: 2 August 2004
Abstract
Canola, (Brassica napus L.) is an important crop in Australia. Large genetic variability in the Australian canola cultivars is reflected by their diverse agronomic characteristics. Further improvement using modern breeding methods will lead to the generation of better canola varieties suited for Australian conditions. Genetic engineering relies on the development of efficient methods for regeneration of viable shoots from cultured tissues, and the successful application of transformation techniques. This study reports the in vitro shoot regeneration potential from seedling explants of 7 commercial genotypes (Dunkeld, Grouse, RK7, RI25, Oscar, Rainbow, and Monty) of Australian canola. Seedling explants of these genotypes were all responsive to shoot regeneration. Total number of shoots regenerated varied significantly among the 7 genotypes. Based on the number of shoots regenerated, Rainbow was found to be the most amenable to in vitro regeneration with 55% of cotyledon explants regenerating 2.47 shoots per explants on shoot initiation medium containing 6-benzylaminopurine (3 mg/L), 1-naphthylacetic acid (0.2 mg/L), and gibberellic acid (0.01 mg/L). Normal fertile canola plants from all the 7 genotypes were regenerated. The results obtained from this study will form the basis for genetic transformation studies.
Additional keywords: plant regeneration, tissue culture.
Acknowledgments
Our sincere thanks to Dr Phil Salisbury and Mr Wayne Burton, VIDA Horsham, for providing seeds of canola genotypes.
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