An enzyme-linked immunosorbent assay for the detection of Rathayibacter toxicus, the bacterium involved in annual ryegrass toxicity, in hay

We thank D. Roberts for supplying the field samples, A. Kelly, G. Dawson, and R. McKenzie of the Queensland Department of Primary Industries and Fisheries for providing the ‘uninfected’ hay, L. den Hollander, D. Hare, M. Kalkhoven, D. Galvin, and N. Lowry for their excellent technical assistance during this study, and Dr J. Allen for advice on the manuscript. The ‘Corynebacterium agropyri’ isolate was kindly provided by Dr Ian Riley. Financial support was provided by the Grains Research Development Corporation and the Rural Industries Research and Development Corporation.

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