Effect of glycerin supplementation on the expression of antioxidant and mitochondrial genes in broilers
R. S. Araújo A , K. R. S. Sousa B E , F. C. B. Sousa A , A. C. Oliveira A , L. R. B. Dourado A , S. E. F. Guimarães C , W. Silva C , D. Biagiotti A , G. F. V. Bayão D and A. C. Araujo A
+ Author Affiliations
- Author Affiliations
A Department of Animal Science, Universidade Federal do Piauí, Campus Profa Cinobelina Elvas, 64900-000, Bom Jesus, PI, Brazil.
B Department of Animal Science, Universidade Federal do Maranhão, 65500-000, Chapadinha, MA, Brazil.
C Department of Animal Science, Animal Biotechnology Laboratory, Universidade Federal de Viçosa, 36900-000 Viçosa, MG, Brazil.
D Department of Animal Science, Instituto Federal de Educação, Ciência e Tecnologia do Amapá, 68997-000, Porto Grande, AP, Brazil.
E Corresponding author. Email: katiregia@gmail.com
Animal Production Science 59(3) 408-415 https://doi.org/10.1071/AN16391
Submitted: 16 June 2016 Accepted: 13 December 2017 Published: 16 March 2018
Abstract
Crude glycerin has been used as an alternative energy source in animal feeding and can thus change both the animal’s performance and the levels of gene expression related to energy production process and the antioxidant system, since the effect of glycerin depends on its degree of purity in the diet. The aim of this study was to evaluate the effect of crude glycerin inclusion (0, 6 and 12%) in the diet has on performance characteristics and mRNA abundance of genes associated with the mitochondrial function (uncoupling protein (UCP), cytochrome C oxidase subunit III (COX III) and adenine nucleotide transporter (ANT)) and those that combat the reactive oxygen species (superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx)) in the liver and muscle of broilers at 42 days of age. Total RNA was extracted from the tissues, and cDNA was amplified using specific primers for the genes under study through real-time reverse transcription polymerase chain reaction; the endogenous gene used was β-actin. There was no difference between glycerin concentrations for weight gain, feed intake or feed conversion. Greater expression of UCP at muscle and higher GPx expression at liver were observed in broilers fed 12% glycerin. In conclusion, the addition of 12% glycerin to broiler diets increased the expression of UCP and GPx, but did not change broiler performance. Therefore, glycerin can be used as an alternative ingredient without losses.
Additional keywords: alternative feedstuffs, functional genomics, poultry nutrition, ROS.
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