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Xylanase and phytase supplementation of Moringa oleifera diets: effects on the performance, egg quality and blood profile of laying hens

Gabriel Macambira , Carlos Rabello , HELIA OLIVEIRA , Marcos Santos , Dayane Silva , Ana Santos, Apolônio Ribeiro , Oziel Lins Júnior, Lucas Sousa, Igor Máximo , Manuel Navarro

Abstract

Context. Moringa oleifera leaves present enormous potential as an alternative feedstuff for laying hens. The utilisation of the leaves can be improved by exogenous enzymes due to the presence of non-starch polysaccharides and phytate. Aim. This study evaluated the influence of Moringa oleifera leaf meal (MOL) in diets supplemented with xylanase and phytase on laying hen performance and egg quality at peak production. Methods. A total of 288 Dekalb White laying hens (32 weeks old) were distributed in a completely randomized design, following a 2 × 4 factorial arrangement (with or without MOL × 4 enzyme supplementations). Each treatment comprised 6 repetitions, each containing 6 hens. The control diet was based on ground corn and soybean meal, and the treatment diets were supplemented with 5% MOL followed by three enzyme additives (xylanase, phytase and a xylanase + phytase blend). Key results. The enzyme supplementation in MOL diets positively influenced egg production (P=0.012), egg weight (P=0.021) and egg mass (P=0.009). The birds offered xylanase supplemented diets had higher feed intakes (P=0.015) than those fed other diets. Moringa oleifera increased the albumen height (P<0.001) and Haugh unit (P<0.001), while MOL and phytase improved eggshell thickness (P<0.001) and the candling score (P=0.049), compared to treatments without phytase supplementation. MOL and enzyme supplementation improved yolk colour intensity (L, A and R parameters) and decreased plasma total cholesterol and LDL cholesterol contents. The inclusion of 5% MOL in diets did not impair laying performance. However, when MOL diets were supplemented with exogenous enzymes, there was an increase in egg weight. Conclusion. MOL can be included at 5% in layer diets without affecting performance. When used in combination with xylanase and phytase, they improve performance, intensify yolk colour, improve shell thickness, candling score, as well as serum biochemical profile. Implications. These findings suggest that the combination of dietary MOL + xylanase + phytase can improve performance and egg quality in laying hens at peak production.

AN24194  Accepted 13 September 2024

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