Liver functionality and its association with milk production and ovarian activity in dairy cows
Arturo Geovany Fuentes-Castillo A , Carolina Bespalhok Jacometo B , José Isaac Norato A , Edil Enrique Araúz-Santamaría A and Pablo Montero-Prado
C *
A
B
C
Abstract
The liver function index (LFI) is a marker that reflects liver function and could be a tool to identify adequate conditions for ovarian functionality and improve milk production capacity during the transition period in the dairy cow.
This study aimed to evaluate LFI according to dietary changes, and its relationship with milk production and the onset of ovarian activity.
Thirty dairy cows were randomly selected into two experimental groups: CON (supplemented with concentrate) and MIX (supplemented with corn and soybean plants silage). Blood samples were taken on the 3rd and 28th days after calving and analyzed for bilirubin (mg/dL), cholesterol (mg/dL), and albumin (g/L) to calculate the LFI. The study variables identified were weight loss at 28 days, milk production at 7 (initial) and 28 days, ovarian and follicular size, and days to first estrus. A two-factor split-plot animal design was used.
The diets (CON or MIX) did not affect any of the studied variables. However, cows with a LFI greater than zero (LFI > 0) had higher initial and 28-day milk production (r = 0.41 and r = 0.80, respectively). Additionally, LFI was positively correlated with transverse (r = 0.28) and longitudinal (r = 0.52) preovulatory follicle size.. Within the first 90 days in milk (DIM), 89% of cows were in estrus, with an average of 49 days until first estrus after calving.
The LFI was correlated with initial milk production at 28 days, but not at 100 days. It was shown that an LFI > 0 influenced ovarian and follicular size, with larger longitudinal and transverse measurements compared to animals with LFI values lower than zero (LFI < 0). The same behavior was observed with preovulatory follicles, with 9 mm for LFI > 0 compared at 4.64 mm for LFI < 0.
The LFI is a potential tool to predict ovarian functionality in terms of the onset of postpartum ovarian activity.
Keywords: fertility problems, follicle size, liver function index, milk, ovarian function, ovulation, postpartum diet, prepartum diet.
References
Alba GLO, Rodriguez GA, Gómez PA, Silveira PEA (2006) Size and shape of the ovaries and cervix in heifers and cows of the absorbent crossbred holstein x zebu). Revista Electrónica de Veterinaria 7, 1-12.
| Google Scholar |
Batista CP, Gonçalves RS, Contreras LVQ, de Faria Valle S, González F (2022) Correlation between liver lipidosis, body condition score variation, and hepatic analytes in dairy cows. Brazilian Journal of Veterinary Medicine 44, e005121.
| Crossref | Google Scholar |
Bauman DE, Mather IH, Wall RJ, Lock AL (2006) Major advances associated with the biosynthesis of milk. Journal of Dairy Science 89(4), 1235-1243.
| Crossref | Google Scholar | PubMed |
Bell A (1995) News time. Time & Society 4(3), 305-328.
| Crossref | Google Scholar |
Bertoni G, Trevisi E (2013) Use of the liver activity index and other metabolic variables in the assessment of metabolic health in dairy herds. Veterinary Clinics of North America: Food Animal Practice 29(2), 413-431.
| Crossref | Google Scholar |
Bertoni G, Trevisi E, Han X, Bionaz M (2008) Effects of inflammatory conditions on liver activity in puerperium period and consequences for performance in dairy cows. Journal of Dairy Science 91(9), 3300-3310.
| Crossref | Google Scholar | PubMed |
Block E (2010) Transition cow research – what makes sense today? In ‘Presented at the high plains dairy conference, arm & hammer animal nutrition’, March 2010, Amarillo, Texas. (Ed. R Hagevoort). Available at Highplainsdairy.org
Bossaert P, Trevisi E, Opsomer G, Bertoni G, De Vliegher S, Leroy JLMR (2012) The association between indicators of inflammation and liver variables during the transition period in high-yielding dairy cows: an observational study. The Veterinary Journal 192(2), 222-225.
| Crossref | Google Scholar | PubMed |
Butler WR (2000) Nutritional interactions with reproductive performance in dairy cattle. Animal Reproduction Science 60–61, 449-457.
| Crossref | Google Scholar |
Campos Gaona R, Correa-Orozco A, Zambrano Burbano GL, Ospina Cordoba PA (2018) Alteraciones bioquímicas y metabólicas en el periodo de transición en vacas lecheras. Revista de Investigación Agraria y Ambiental 9(2), 165-179.
| Crossref | Google Scholar |
Cardoso FC, LeBlanc SJ, Murphy MR, Drackley JK (2013) Prepartum nutritional strategy affects reproductive performance in dairy cows. Journal of Dairy Science 96(9), 5859-5871.
| Crossref | Google Scholar | PubMed |
Cardoso FC, Kalscheur KF, Drackley JK (2020) Symposium review: Nutrition strategies for improved health, production, and fertility during the transition period. Journal of Dairy Science 103(6), 5684-5693.
| Crossref | Google Scholar | PubMed |
Cheong SH, Filho OGS, Absalón-Medina VA, Pelton SH, Butler WR, Gilbert RO (2016) Metabolic and endocrine differences between dairy cows that do or do not ovulate first postpartum dominant follicles. Biology of Reproduction 94(1), 1-11.
| Crossref | Google Scholar |
Crowe MA, Diskin MG, Williams EJ (2014) Parturition to resumption of ovarian cyclicity: comparative aspects of beef and dairy cows. Animal 8, 40-53.
| Crossref | Google Scholar |
Curone G, Filipe J, Cremonesi P, Trevisi E, Amadori M, Pollera C, Castiglioni B, Turin L, Tedde V, Vigo D, Moroni P, Minuti A, Bronzo V, Addis MF, Riva F (2018) What we have lost: mastitis resistance in Holstein Friesians and in a local cattle breed. Research in Veterinary Science 116, 88-98.
| Crossref | Google Scholar |
Dobson H, Smith R, Royal M, Knight C, Sheldon I (2007) The high producing dairy cow and its reproductive performance. Reproduction in Domestic Animals 42, 17-23.
| Crossref | Google Scholar |
Drackley JK, Cardoso FC (2014) Prepartum and postpartum nutritional management to optimize fertility in high-yielding dairy cows in confined TMR systems. Animal 8, 5-14.
| Crossref | Google Scholar | PubMed |
Drackley JK, Donkin SS, Reynolds CK (2006) Major advances in fundamental dairy cattle nutrition. Journal of Dairy Science 89(4), 1324-1336.
| Crossref | Google Scholar | PubMed |
Geary T (2003) What makes Flossie fertile? Can egg follicle size indicate cow fertility? Agricultural Research Magazine, United States Department of Agriculture. Available at https://agresearchmag.ars.usda.gov/2003/apr/cow/
Gill JL (1988) Standard errors for split-split-plot experiments with repeated measurements of animals. Journal of Animal Breeding and Genetics 105(1–6), 329-336.
| Crossref | Google Scholar |
Girma S, Fikadu T, Agdew E, Haftu D, Gedamu G, Dewana Z, Getachew B (2019) Factors associated with low birthweight among newborns delivered at public health facilities of Nekemte town, West Ethiopia: a case control study. BMC Pregnancy and Childbirth 19, 220.
| Crossref | Google Scholar |
Gobikrushanth M, Macmillan K, Behrouzi A, Hoff B, Colazo MG (2019) The factors associated with postpartum body condition score change and its relationship with serum analytes, milk production and reproductive performance in dairy cows. Livestock Science 228, 151-160.
| Crossref | Google Scholar |
Gong JG, Lee WJ, Garnsworthy PC, Webb R (2002) Effect of dietary-induced increases in circulating insulin concentrations during the early postpartum period on reproductive function in dairy cows. Reproduction 123(3), 419-427.
| Crossref | Google Scholar | PubMed |
Gross JJ (2023) Dairy cow physiology and production limits. Animal Frontiers 13, 44-50.
| Crossref | Google Scholar |
Grummer RR (2008) Nutritional and management strategies for the prevention of fatty liver in dairy cattle. The Veterinary Journal 176, 10-20.
| Crossref | Google Scholar |
Grummer RR, Mashek DG, Hayirli A (2004) Dry matter intake and energy balance in the transition period: managing the transition cow to optimize health and productivity. Veterinary Clinics of North America: Food Animal Practice 20, 447-470.
| Crossref | Google Scholar |
Kelly AK, Byrne C, McGee M, Perry GA, Crowe MA, Sauerwein H, Kenny DA (2020) Effect of calfhood nutrition on metabolic hormones, gonadotropins, and estradiol concentrations and on reproductive organ development in beef heifer calves. Journal of Animal Science 98, skaa310.
| Crossref | Google Scholar |
Landau S, Braw-Tal R, Kaim M, Bor A, Bruckental I (2000) Preovulatory follicular status and diet affect the insulin and glucose content of follicles in high-yielding dairy cows. Animal Reproduction Science 64(3–4), 181-197.
| Crossref | Google Scholar | PubMed |
Lucy MC (2000) Regulation of ovarian follicular growth by somatotropin and insulin-like growth factors in cattle. Journal of Dairy Science 83(7), 1635-1647.
| Crossref | Google Scholar | PubMed |
Lucy MC (2008) Functional differences in the growth hormone and insulin-like growth factor axis in cattle and pigs: implications for post-partum nutrition and reproduction. Reproduction in Domestic Animals 43(S2), 31-39.
| Crossref | Google Scholar |
Lucy MC, Savio JD, Badinga L, De La Sota RL, Thatcher WW (1992) Factors that affect ovarian follicular dynamics in cattle. Journal of Animal Science 70(11), 3615-3626.
| Crossref | Google Scholar | PubMed |
Lucy MC, Butler ST, Garverick HA (2014) Endocrine and metabolic mechanisms linking postpartum glucose with early embryonic and foetal development in dairy cows. Animal 8(1), 82-90.
| Crossref | Google Scholar | PubMed |
Marin D, Bazeos A, Mahon F-X, Eliasson L, Milojkovic D, Bua M, Apperley JF, Szydlo R, Desai R, Kozlowski K, Paliompeis C, Latham V, Foroni L, Molimard M, Reid A, Rezvani K, de Lavallade H, Guallar C, Goldman J, Khorashad JS (2010) Adherence is the critical factor for achieving molecular responses in patients with chronic myeloid leukemia who achieve complete cytogenetic responses on imatinib. Journal of Clinical Oncology 28, 2381-2388.
| Crossref | Google Scholar |
Mekuriaw Y (2023) Negative energy balance and its implication on productive and reproductive performance of early lactating dairy cows: review paper. Journal of Applied Animal Research 51, 220-228.
| Crossref | Google Scholar |
Montagner A, Korecka A, Polizzi A, Lippi Y, Blum Y, Canlet C, Tremblay-Franco M, Gautier-Stein A, Burcelin R, Yen Y-C, Je HS, Al-Asmakh M, Mithieux G, Arulampalam V, Lagarrigue S, Guillou H, Pettersson S, Wahli W (2016) Hepatic circadian clock oscillators and nuclear receptors integrate microbiome-derived signals. Scientific Reports 6(1), 20127.
| Crossref | Google Scholar | PubMed |
Nett TM, Turzillo AM, Baratta M, Rispoli LA (2002) Pituitary effects of steroid hormones on secretion of follicle-stimulating hormone and luteinizing hormone. Domestic Animal Endocrinology 23, 33-42.
| Crossref | Google Scholar |
Nigussie T (2018) A review on the role of energy balance on reproduction of dairy cow. Journal of Dairy Research & Technology 1, 003.
| Crossref | Google Scholar |
NRC (2001) ‘Nutrient Requirements of Dairy Cattle’, Seventh revised edn, National Research Council. ed. (The National Academies Press: Washington, DC, USA). 10.17226/9825
Roche JF (2006) The effect of nutritional management of the dairy cow on reproductive efficiency. Animal Reproduction Science 96(3–4), 282-296.
| Crossref | Google Scholar |
Rukkwamsuk T, Wensing T, Kruip TAM (1999) Relationship between triacylglycerol concentration in the liver and first ovulation in postpartum dairy cows. Theriogenology 51(6), 1133-1142.
| Crossref | Google Scholar | PubMed |
Sangsritavong S, Combs DK, Sartori R, Armentano LE, Wiltbank MC (2002) High feed intake increases liver blood flow and metabolism of progesterone and estradiol-17β in dairy cattle. Journal of Dairy Science 85(11), 2831-2842.
| Crossref | Google Scholar | PubMed |
Song X, Huang X, Xu H, Zhang C, Chen S, Liu F, Guan S, Zhang S, Zhu K, Wu C (2020) The prevalence of pathogens causing bovine mastitis and their associated risk factors in 15 large dairy farms in China: an observational study. Veterinary Microbiology 247, 108757.
| Crossref | Google Scholar |
Wiener-Lab (2000a) Colorimetric method for the determination of albumin in serum (No. UR230712). https://access.wiener-lab.com/VademecumDocumentos/Vademecum%20espanol/albumina_aa_sp.pdf
Wiener-Lab (2000b) Enzymatic method for determining cholesterol in serum or plasma (No. UR190508). https://www.distribuidoravisalud.com/vademecum/colestat_enzimatico_aa_liquida_en.pdf
Wiener-Lab (2000c) DPD method for total bilirubin determination in serum or plasma (No. UR170619). https://access.wiener-lab.com/VademecumDocumentos/Vademecum%20ingles/bilirrubina_total_aa_en.pdf
Wiltbank MC, Fricke PM, Sangsritavong S, Sartori R, Ginther OJ (2000) Mechanisms that prevent and produce double ovulations in dairy cattle. Journal of Dairy Science 83(12), 2998-3007.
| Crossref | Google Scholar | PubMed |
Wittwer F (2015) Marcadores bioquímicos sanguíneos en el diagnóstico y control de trastornos metabólicos en vacas lecheras. In ‘Presented at the anais do 2o simpósio nacional da vaca leiteira’, October 2015, Porto alegre. (Eds FHD González, PM Mallmann Júnior) pp. 34–62. (Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul: Porto Alegre)
Zhou X, Mayes HB, Broadbelt LJ, Nolte MW, Shanks BH (2016) Fast pyrolysis of glucose-based carbohydrates with added NaCl part 2: validation and evaluation of the mechanistic model. AIChE Journal 62, 778-791.
| Crossref | Google Scholar |
