Choice of companion legume influences lamb liveweight output and grain yields in a dual use perennial wheat/legume intercrop system

Matthew T. Newell; Richard C. Hayes; Gordon Refshauge; Benjamin W. B. Holman; Neil Munday; David L. Hopkins; Li Guangdi

doi:10.1071/AN24246

RESEARCH ARTICLE (Open Access)

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Choice of companion legume influences lamb liveweight output and grain yields in a dual use perennial wheat/legume intercrop system

Matthew T. Newell

^A ^* , Richard C. Hayes ^B , Gordon Refshauge ^A ^C , Benjamin W. B. Holman

^B ^C , Neil Munday ^D , David L. Hopkins ^C and Li Guangdi ^B

+ Author Affiliations

- Author Affiliations

^A Cowra Agricultural Research and Advisory Station, NSW Department of Primary Industries and Regional Development, Cowra, NSW 2794, Australia.

^B Wagga Wagga Agricultural Institute, NSW Department of Primary Industries and Regional Development, Wagga Wagga, NSW 2650, Australia.

^C Centre for Red Meat and Sheep Development, NSW Department of Primary Industries and Regional Development, Cowra, NSW 2794, Australia.

^D Orange Agricultural Institute, NSW Department of Primary Industries and Regional Development, Orange, NSW 2800, Australia.

^* Correspondence to: matt.newell@dpi.nsw.gov.au

Handling Editor: Stephanie Muir

Animal Production Science 65, AN24246 https://doi.org/10.1071/AN24246

Submitted: 29 July 2024 Accepted: 12 February 2025 Published: 3 March 2025

© 2025 The Author(s) (or their employer(s)). Published by CSIRO Publishing. This is an open access article distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND)

Abstract

Context

Perennial cereals are being developed for dual roles of forage and grain production. Like other cereals, perennial wheat (PW) forage requires mineral supplementation if grazed by ruminants.

Aims

To investigate the effect on liveweight gain in lambs grazing PW/legume intercrops in comparison to grazing PW with a mineral supplement. Effects of intercropping and impact of grazing on PW grain yield were also investigated.

Methods

Lambs (14-week-old, n = 144) grazed one of four treatments, namely PW with a mineral supplement (PW + Min) or PW intercropped with either lucerne (Medicago sativa) (PW + L), subterranean clover (Trifolium subterraneum) (PW + C), or French serradella (Ornithopus sativus) (PW + S) for 12 weeks. Treatments were arranged in a randomised block design with six replicates. Following grazing, grain yield from each treatment was compared with an ungrazed control upon maturity.

Key results

Intercropping with either subterranean clover or French serradella increased carrying capacity and total liveweight grain, similar to the PW + Min treatment and supported a higher stocking rate compared with the PW + L treatment. Sodium concentration was approximately 10-fold higher in the herbage of subterranean clover and serradella compared with PW, and 5-fold higher than lucerne. Grain yields from intercropping were lower compared with PW + Min due to the reduction in perennial wheat density. However, proportionally, PW grain yield was improved in the PW + S and PW + L treatments with a Net Effect Ratio (NER) > 1.

Conclusions

Increased feed availability from the provision of forages, such as subterranean clover and French serradella, enabled greater liveweight output through greater carrying capacity of grazing lambs when compared with a PW + L diet. However these were not different to PW + Min. Improved sodium intake is also implicated in this result, however not confirmed by this study. Grain yields were not affected by grazing, although they were reduced by intercropping. However, the comparative improvement in PW grain yield (NER) in combination with a compatible legume, along with increased grazing days, highlight the potential of intercropping where more than one product is produced in a multi-functional, dual-purpose perennial grain system.

Implications

The comparative improvement in grain yield from intercropping, coupled with increased liveweight change, supports the use of compatible legume intercrops in dual-purpose perennial grain systems.

Keywords: alfalfa, forage minerals, Land Equivalent Ratio, Net Effect Ratio, perennial cereals, perennial crops, serradella, subterranean clover.

References

AFIA (2014) ‘AFIA laboratory methods manual.’ (Australian Fodder Industry Association Limited: St Kilda, Victoria)

Atabo JA, Umaru TM (2015) Assessing the land equivalent ratio (LER) and stability of yield of two cultivars of sorghum (Sorghum bicolor L. Moench)-Soyabean (Glycine max L. Merr) to row intercropping system. Journal of Biology, Agriculture and Healthcare 5(18), 144-149.
| Google Scholar |

Bell LW (2013) Economics and system applications for perennial grain crops in dryland farming systems in Australia. In ‘Perennial crops for food sedurity. Proceedings of FAO expert workshop’, 28–30 August, Rome. (Eds C Batello, L Wade, S Cox, N Pogna, A Bozzini, J Choptiany) pp. 169–186. (FAO: Rome)

Bell LW, Moore AD, Kirkegaard JA (2014) Evolution in crop–livestock integration systems that improve farm productivity and environmental performance in Australia. European Journal of Agronomy 57, 10-20.
| Crossref | Google Scholar |

Betencourt E, Duputel M, Colomb B, Desclaux D, Hinsinger P (2012) Intercropping promotes the ability of durum wheat and chickpea to increase rhizosphere phosphorus availability in a low P soil. Soil Biology and Biochemistry 46, 181-190.
| Crossref | Google Scholar |

Block E (1984) Manipulating dietary anions and cations for prepartum dairy cows to reduce incidence of milk fever. Journal of Dairy Science 67(12), 2939-2948.
| Crossref | Google Scholar | PubMed |

Butler D (2020) ‘Package ‘ASReml’ 4.1.0.130.’ (VSN International: Hemel Hempstead, UK)

Cann DJ, Hunt JR, Porker KD, Harris FAJ, Rattey A, Hyles J (2023) The role of phenology in environmental adaptation of winter wheat. European Journal of Agronomy 143, 126686.
| Crossref | Google Scholar |

Chalmers S (2014) Intercropping pea and canola based on row orientation and nitrogen rates final report 2011–2013. Westman Agricultural Diversification Organistaion 2014 Annual Report. Westman Agricultural Diversification Organisation, Manitoba.

Champness MR, McCormick JI, Bhanugopan MS, McGrath SR (2021) Sodium deficiency in lucerne (Medicago sativa) forage in southern Australia and the effect of sodium and barley supplementation on the growth rate of lambs grazing lucerne. Animal Production Science 61(11), 1170-1180.
| Crossref | Google Scholar |

Chapagain T, Riseman A (2014) Barley–pea intercropping: effects on land productivity, carbon and nitrogen transformations. Field Crops Research 166, 18-25.
| Crossref | Google Scholar |

Coombes N (2020) DiGGer: DiGGer design generator under correlation and blocking. R Package Version 1.0.5. Available at http://nswdpibiom.org/austatgen/software

Cox CM, Murray TD, Jones SS (2002) Perennial wheat germ plasm lines resistant to eyespot, Cephalosporium stripe, and wheat streak mosaic. Plant Disease 86(9), 1043-1048.
| Crossref | Google Scholar | PubMed |

Crews TE, Blesh J, Culman SW, Hayes RC, Jensen ES, Mack MC, Peoples MB, Schipanski ME (2016) Going where no grains have gone before: from early to mid-succession. Agriculture, Ecosystems & Environment 223, 223-238.
| Crossref | Google Scholar |

Crews TE, Carton W, Olsson L (2018) Is the future of agriculture perennial? Imperatives and opportunities to reinvent agriculture by shifting from annual monocultures to perennial polycultures. Global Sustainability 1, e11.
| Crossref | Google Scholar |

Crews TE, Kemp L, Bowden JH, Murrell EG (2022) How the nitrogen economy of a perennial cereal-legume intercrop affects productivity: can synchrony be achieved? Frontiers in Sustainable Food Systems 6, 755548.
| Crossref | Google Scholar |

Darch T, McGrath SP, Lee MRF, Beaumont DA, Blackwell MSA, Horrocks CA, Evans J, Storkey J (2020) The mineral composition of wild-type and cultivated varieties of pasture species. Agronomy 10(10), 1463.
| Crossref | Google Scholar |

Daryanto S, Fu B, Zhao W, Wang S, Jacinthe P-A, Wang L (2020) Ecosystem service provision of grain legume and cereal intercropping in Africa. Agricultural Systems 178, 102761.
| Crossref | Google Scholar |

Dick C, Cattani D, Entz MH (2018) Kernza intermediate wheatgrass (Thinopyrum intermedium) grain production as influenced by legume intercropping and residue management. Canadian Journal of Plant Science 98(6), 1376-1379.
| Crossref | Google Scholar |

Dove H, Kirkegaard J (2014) Using dual-purpose crops in sheep-grazing systems. Journal of the Science of Food and Agriculture 94(7), 1276-1283.
| Crossref | Google Scholar | PubMed |

Dove H, McMullen KG (2009) Diet selection, herbage intake and liveweight gain in young sheep grazing dual-purpose wheats and sheep responses to mineral supplements. Animal Production Science 49(10), 749-758.
| Crossref | Google Scholar |

Dove H, Holst P, Stanley D, Flint P (2002) Grazing value of dual-purpose winter wheats for young sheep. Proceedings of the Australian Society of Animal Production 24, 53-56.
| Google Scholar |

Dove H, Kirkegaard JA, Kelman WM, Sprague SJ, McDonald SE, Graham JM (2015) Integrating dual-purpose wheat and canola into high-rainfall livestock systems in south-eastern Australia. 2. Pasture and livestock production. Crop & Pasture Science 66(4), 377-389.
| Crossref | Google Scholar |

Dove H, Masters DG, Thompson AN (2016) New perspectives on the mineral nutrition of livestock grazing cereal and canola crops. Animal Production Science 56(8), 1350-1360.
| Crossref | Google Scholar |

Fletcher AL, Kirkegaard JA, Peoples MB, Robertson MJ, Whish J, Swan AD (2016) Prospects to utilise intercrops and crop variety mixtures in mechanised, rain-fed, temperate cropping systems. Crop & Pasture Science 67(12), 1252-1267.
| Crossref | Google Scholar |

Floate MJS (1981) Effects of grazing by large herbivores on nitrogen cycling in agricultural ecosystems. Ecological Bulletins 33, 585-601.
| Google Scholar |

Harris D, Natarajan M, Willey RW (1987) Physiological basis for yield advantage in a sorghum/groundnut intercrop exposed to drought. 1. Dry-matter production, yield, and light interception. Field Crops Research 17(3–4), 259-272.
| Crossref | Google Scholar |

Harrison MT, Evans JR, Dove H, Moore AD (2011a) Dual-purpose cereals: can the relative influences of management and environment on crop recovery and grain yield be dissected? Crop & Pasture Science 62(11), 930-946.
| Crossref | Google Scholar |

Harrison MT, Evans JR, Dove H, Moore AD (2011b) Recovery dynamics of rainfed winter wheat after livestock grazing 1. Growth rates, grain yields, soil water use and water-use efficiency. Crop & Pasture Science 62(11), 947-959.
| Crossref | Google Scholar |

Harrison MT, Evans JR, Dove H, Moore AD (2011c) Recovery dynamics of rainfed winter wheat after livestock grazing 2. Light interception, radiation-use efficiency and dry-matter partitioning. Crop & Pasture Science 62(11), 960-971.
| Crossref | Google Scholar |

Hayes RC, Dear BS, Orchard BA, Peoples M, Eberbach PL (2008) Response of subterranean clover, balansa clover, and gland clover to lime when grown in mixtures on an acid soil. Australian Journal of Agricultural Research 59(9), 824-835.
| Crossref | Google Scholar |

Hayes RC, Newell MT, DeHaan LR, Murphy KM, Crane S, Norton MR, Wade LJ, Newberry M, Fahim M, Jones SS, Cox TS, Larkin PJ (2012) Perennial cereal crops: an initial evaluation of wheat derivatives. Field Crops Research 133, 68-89.
| Crossref | Google Scholar |

Hayes RC, Newell MT, Crews TE, Peoples MB (2017) Perennial cereal crops: an initial evaluation of wheat derivatives grown in mixtures with a regenerating annual legume. Renewable Agriculture and Food Systems 32(3), 276-290.
| Crossref | Google Scholar |

Hayes RC, Wang S, Newell MT, Turner K, Larsen J, Gazza L, Anderson JA, Bell LW, Cattani DJ, Frels K, Galassi E, Morgounov AI, Revell CK, Thapa DB, Sacks EJ, Sameri M, Wade LJ, Westerbergh A, Shamanin V, Amanov A, Li GD (2018) The performance of early-generation perennial winter cereals at 21 sites across four continents. Sustainability 10(4), 1124.
| Crossref | Google Scholar |

Hayes RC, Newell MT, Pembleton KG, Peoples MB, Li GD (2021) Sowing configuration affects competition and persistence of lucerne (Medicago sativa) in mixed pasture swards. Crop & Pasture Science 72, 707-722.
| Crossref | Google Scholar |

Hayes RC, Newell MT, Swan AD, Peoples MB, Pembleton KG, Li GD (2022) Consequences of changing spatial configuration at sowing in the transitions between crop and pasture phases. Journal of Agronomy and Crop Science 208(3), 394-412.
| Crossref | Google Scholar |

Hayes RC, Li GD, Smith RW, Peoples MB, Rawnsley RP, Newell MT, Pembleton KG (2024) Prospects for improving productivity and composition of mixed swards in semi-arid environments by separating species in drill rows – a review. Agriculture, Ecosystems & Environment 373, 109131.
| Crossref | Google Scholar |

Hunter MC, Sheaffer CC, Culman SW, Jungers JM (2020) Effects of defoliation and row spacing on intermediate wheatgrass I: Grain production. Agronomy Journal 112(3), 1748-1763.
| Crossref | Google Scholar |

Isbell RF (1996) ‘The Australian soil classification.’ (CSIRO Publishing: Melbourne)

Juknevičius S, Sabienė N (2007) The content of mineral elements in some grasses and legumes. Ekologija 53(1), 44-52.
| Google Scholar |

Kemp A, ‘t Hart ML (1957) Grass tetany in grazing milking cows. Netherlands Journal of Agricultural Science 5(1), 4-17.
| Crossref | Google Scholar |

Khanal U, Stott KJ, Armstrong R, Nuttall JG, Henry F, Christy BP, Mitchell M, Riffkin PA, Wallace AJ, McCaskill M, Thayalakumaran T, O’Leary GJ (2021) Intercropping – evaluating the advantages to broadacre systems. Agriculture 11(5), 453.
| Crossref | Google Scholar |

Larkin PJ, Newell MT, Hayes RC, Aktar J, Norton MR, Moroni SJ, Wade LJ (2014) Progress in developing perennial wheats for grain and grazing. Crop & Pasture Science 65(11), 1147-1164.
| Crossref | Google Scholar |

Lee MA (2018) A global comparison of the nutritive values of forage plants grown in contrasting environments. Journal of Plant Research 131, 641-654.
| Crossref | Google Scholar | PubMed |

Lefroy EC, Stirzaker RJ (1999) Agroforestry for water management in the cropping zone of southern Australia. Agroforestry Systems 45, 277-302.
| Crossref | Google Scholar |

Li C, Hoffland E, Kuyper TW, Yu Y, Zhang C, Li H, Zhang F, van der Werf W (2020) Syndromes of production in intercropping impact yield gains. Nature Plants 6, 653-660.
| Crossref | Google Scholar | PubMed |

Li C, Stomph T-J, Makowski D, Li H, Zhang C, Zhang F, van der Werf W (2023) The productive performance of intercropping. Proceedings of the National Academy of Sciences 120(2), e2201886120.
| Crossref | Google Scholar | PubMed |

Masters DG (2018) Practical implications of mineral and vitamin imbalance in grazing sheep. Animal Production Science 58(8), 1438-1450.
| Crossref | Google Scholar |

Masters DG, Thompson AN (2016) Grazing crops: implications for reproducing sheep. Animal Production Science 56(4), 655-668.
| Crossref | Google Scholar |

Masters DG, Hancock S, Refshauge G, Robertson SM, McGrath S, Bhanugopan M, Friend MA, Thompson AN (2019) Mineral supplements improve the calcium status of pregnant ewes grazing vegetative cereals. Animal Production Science 59(7), 1299-1309.
| Crossref | Google Scholar |

McGrath SR, Pinares-Patiño CS, McDonald SE, Kirkegaard JA, Simpson RJ, Moore AD (2021a) Utilising dual-purpose crops in an Australian high-rainfall livestock production system to increase meat and wool production. 1. Forage production and crop yields. Animal Production Science 61, 1062-1073.
| Crossref | Google Scholar |

McGrath SR, Behrendt R, Friend MA, Moore AD (2021b) Utilising dual-purpose crops effectively to increase profit and manage risk in meat production systems. Animal Production Science 61(11), 1049-1061.
| Crossref | Google Scholar |

National Research Council (2007) ‘Nutrient requirements of small ruminants: sheep, goats, cervids, and new world camelids.’ (National Research Council: Washington, DC)

Naudin C, Corre-Hellou G, Pineau S, Crozat Y, Jeuffroy M-H (2010) The effect of various dynamics of N availability on winter pea–wheat intercrops: crop growth, N partitioning and symbiotic N2 fixation. Field Crops Research 119(1), 2-11.
| Crossref | Google Scholar |

Newell MT, Hayes RC (2017) An initial investigation of forage production and feed quality of perennial wheat derivatives. Crop & Pasture Science 68(12), 1141-1148.
| Crossref | Google Scholar |

Newell MT, Holman BWB, Refshauge G, Shanley AR, Hopkins DL, Hayes RC (2020a) The effect of a perennial wheat and lucerne biculture diet on feed intake, growth rate and carcass characteristics of Australian lambs. Small Ruminant Research 192, 106235.
| Crossref | Google Scholar |

Newell M, Holman B, Refshauge G, Hopkins D, Hayes R (2020b) Novel dual purpose perennial wheat for grazing. In ‘2020 ASA-CSSA-SSSA international annual meeting virtual’, November 9–13. (American Society of Agronomy | Crop Science Society of America | Soil Science Society of America)

Oddy H, Allan A (2001) The importance of feed quality to animal performance. In ‘From feast to famine: balancing the pasture feed supply. Proceedings of the 16th annual conference’, 25 July, Grassland Society NSW, Gundagai. (Grassland Society NSW Inc)

Peoples MB, Brockwell J, Hunt JR, Swan AD, Watson L, Hayes RC, Li GD, Hackney B, Nuttall JG, Davies SL, Fillery IRP (2012) Factors affecting the potential contributions of N2 fixation by legumes in Australian pasture systems. Crop and Pasture Science 63, 759-786.
| Crossref | Google Scholar |

Provenza FD, Villalba JJ, Dziba LE, Atwood SB, Banner RE (2003) Linking herbivore experience, varied diets, and plant biochemical diversity. Small Ruminant Research 49(3), 257-274.
| Crossref | Google Scholar |

Refshauge G, Newell MT, Hopkins DL, Holman BWB, Morris S, Hayes RC (2022) The plasma and urine mineral status of lambs offered diets of perennial wheat or annual wheat, with or without lucerne. Small Ruminant Research 209, 106639.
| Crossref | Google Scholar |

Ryan MR, Crews TE, Culman SW, DeHaan LR, Hayes RC, Jungers JM, Bakker MG (2018) Managing for multifunctionality in perennial grain crops. BioScience 68(4), 294-304.
| Crossref | Google Scholar | PubMed |

Smith GS, Middleton KR, Edmonds AS (1978) A classification of pasture and fodder plants according to their ability to translocate sodium from their roots into aerial parts. New Zealand Journal of Experimental Agriculture 6(3), 183-188.
| Crossref | Google Scholar |

SPAC (1998) Soil and plant analysis council. In ‘Handbook of reference methods for plant analysis.’ (Ed. YP Kalra) p. 320. (CRC press: Washington DC)

Sprague SJ, Kirkegaard JA, Bell LW, Seymour M, Graham J, Ryan M (2018) Dual-purpose cereals offer increased productivity across diverse regions of Australia’s high rainfall zone. Field Crops Research 227, 119-131.
| Crossref | Google Scholar |

Stott KJ, Wallace AJ, Khanal U, Christy BP, Mitchell ML, Riffkin PA, McCaskill MR, Henry FJ, May MD, Nuttall JG, O’Leary GJ (2023) Intercropping-towards an understanding of the productivity and profitability of dryland crop mixtures in Southern Australia. Agronomy 13(10), 2510.
| Crossref | Google Scholar |

Takagi H, Block E (1991) Effects of various dietary cation-anion balances on response to experimentally induced hypocalcemia in sheep. Journal of Dairy Science 74(12), 4215-4224.
| Crossref | Google Scholar | PubMed |

Tautges NE, Jungers JM, DeHaan LR, Wyse DL, Sheaffer CC (2018) Maintaining grain yields of the perennial cereal intermediate wheatgrass in monoculture v. bi-culture with alfalfa in the Upper Midwestern USA. The Journal of Agricultural Science 156(6), 758-773.
| Crossref | Google Scholar |

Virgona JM, Gummer FAJ, Angus JF (2006) Effects of grazing on wheat growth, yield, development, water use, and nitrogen use. Australian Journal of Agricultural Research 57(12), 1307-1319.
| Crossref | Google Scholar |

Walker R, Pappa V, Rees R, Baddeley J, Watson C (2009) Effects of cereal/legume intercrops within a rotation. ‘18th symposium of the international scientific centre of fertilizers,’ 8–12 November 2009, Rome, Italy. (Ministry of Agriculture Food and Forestry Policies)

Wheeler DM, Dodd MB (1995) Effect of aluminium on yield and plant chemical concentrations of some temperate legumes. Plant and Soil 173, 133-145.
| Crossref | Google Scholar |

Zadoks JC, Chang TT, Konzak CF (1974) A decimal code for the growth stages of cereals. Weed Research 14(6), 415-421.
| Crossref | Google Scholar |

‘t Mannetje L (1978) Measuring quantity of grassland vegetation. In ‘Measurement of grassland vegetation and animal production’. (Ed. L ‘t Mannetje) pp. 63–91. (Commonwealth Agricultural Bureaux: Hurley, UK)