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RESEARCH ARTICLE

Effects of rumen-protected fat on performance, carcass and meat characteristics, and feeding behaviour of Nellore bulls finished in intensive grazing systems

Edjane Pereira da Silva A , Joanis Tilemahos Zervoudakis B , Osvaldo Alex de Sousa C , Mozart Alves Fonseca D , Danilo Vinicius da Silva Pereira A , Franciely de Oliveira Dutra E , Emanoelly Auxiliadora Paes Monge A , Vitória de Almeida e Silva F , Deborá Kemelly Xavier da Mata F and Nelcino Francisco de Paula https://orcid.org/0000-0002-0392-5653 A *
+ Author Affiliations
- Author Affiliations

A Faculdade de Agronomia e Zootecnia, Universidade Federal de Mato Grosso, Cuiabá, MT 78060-900, Brazil.

B Faculdade de Medicina Veterinária, Universidade Federal de Mato Grosso, Cuiabá, MT 78060-900, Brazil.

C Nutricorp, Araras, SP 13601-000, Brazil.

D Department of Agriculture, Veterinary & Rangeland Sciences, University of Nevada, Reno, NV 89557, USA.

E Faculdade de Zootecnia, Universidade do Estado de Mato Grosso, Pontes e Lacerda, MT 78250-000, Brazil.

F Faculdade de Medicina Veterinária, Universidade de Cuiabá, Cuiabá, MT 78065-900, Brazil.

* Correspondence to: nelcino.paula@ufmt.br

Handling Editor: D. Y. Wang

Animal Production Science 64, AN23408 https://doi.org/10.1071/AN23408
Submitted: 13 December 2023  Accepted: 20 July 2024  Published: 12 August 2024

© 2024 The Author(s) (or their employer(s)). Published by CSIRO Publishing

Abstract

Context

Beef cattle production in the tropics is based on the grazing system. Concentrate supplementation has a significant influence on carcass gain and meat quality. Additionally, the inclusion of some lipid feedstuffs, such as rumen-protected fat (RPF), can increase the net energy for weight gain, and be an alternative to increase the productive performance and carcass traits of grazing beef cattle.

Aims

This study evaluated the effects of RPF on performance, carcass traits and feeding behaviour of Nellore bulls in an intensive grazing system during the rainy season.

Methods

Twenty-four Nellore bulls (initial bodyweight of 454.0 ± 46.1 kg) were used in a randomised completed block design, based on initial bodyweight. The animals were distributed in 12 paddocks (two bulls per paddock) in a continuous grazing system and randomly assigned to one of two treatments: control (n = 6), a concentrate without added protected fat provided ad libitum, and RPF (n = 6), a concentrate with the addition of 3% protected fat (Nutricorp, Araras, São Paulo, Brazil) provided ad libitum. Statistical analysis was performed using the MIXED procedure of SAS 9.4 with paddock as the experimental unit (12 paddocks). The principal model included fixed effect of treatment and block as a random effect. For forage characteristics, the experimental period was a considered fixed effect. The study lasted 105 days, and was divided into four periods. At the end of each period, forage collections were performed, bulls were weighed and feeding behaviour was recorded. After 105 days, the bulls were slaughtered in a commercial slaughterhouse, and three 2.54-cm Longissimus dorsi steaks of each animal were collected to evaluate the colour, the chemical and physical composition, and the sensory analysis of the meat.

Keys results

The inclusion of RPF in the concentrate did not affect (P > 0.05) the final bodyweight, average daily gain, concentrate intake and feed efficiency, but there was a tendency (P < 0.07) for higher carcass weight for the bulls that received RPF. Bulls supplemented with RPF had a greater (P < 0.05) average carcass gain. For meat quality, an effect (P < 0.01) was observed for hue (25.32 and 27.91 for control and RPF, respectively).

Conclusions

The addition of 3% RPF in the concentrate ration has the potential to improve carcass traits and meat quality of beef cattle in an intensive pasture finishing system.

Implications

The inclusion of RPF will increase dietary energy density of grazing beef cattle with a tendency to increase carcass gain. This can be an opportunity to increase the production per animal and income of farmers.

Keywords: calcium salts, fatty acids, grazing, meat quality, nutrition, pasture finishing, systems, tropics.

References

ABIEC (2022) Exportação Brasileira de Carne Bovina. Associação Brasileira das Indústrias Exportadoras de Carne. Available at https://www.abiec.com.br/publicacoes/beef-report-2022/

AOCS (2005) Official Procedures of the American Oil Chemists Society, Approved Procedure Am 5-04, Rapid determination of oil/fat utilizing high temperature solvent extraction. The Society: Urbana, IL. Available at https://img.21food.cn/img/biaozhun/20100108/187/11286046.pdf

Behan AA, Loh TC, Fakurazi S, Kaka U, Kaka A, Samsudin AA (2019) Effects of supplementation of rumen protected fats on rumen ecology and digestibility of nutrients in sheep. Animals 9(7), 400.
| Crossref | Google Scholar | PubMed |

Bindari YR, Shrestha S, Shrestha N, Gaire TN (2013) Effects of nutrition on reproduction: a review. Advances in Applied Science Research 4(1), 421-429 Available at https://www.cabdirect.org/cabdirect/abstract/20133114938.
| Google Scholar |

Biserra TT, Cecato U, Galbeiro S, Bridi AM, Fernandes HJ, Duarte CFD, Da Silva DR, Vicente JVR, Rogel CP (2020) The effect of different grazing heights on carcass and meat quality characteristics of Nellore cattle fattened in pastures of Convert grass. Tropical Animal Health and Production 52, 625-635.
| Crossref | Google Scholar | PubMed |

Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology 37, 911-917.
| Crossref | Google Scholar | PubMed |

BR-CORTE (2016) ‘Cálculo de exigências nutricionais, formulação de dietas e predição de desempenho de zebuínos puros e cruzados.’ 3 edn. p. 142. (UFV/DZO: Viçosa) Available at https://brcorte.com.br/assets/book2016/br/c0.pdf

Brandão AP, Cooke RF, Schubach KM, Marques RS, Bohnert DW, Mercadante VRG (2018) Supplementing calcium salts of soybean oil after artificial insemination increases pregnancy success in Bos taurus beef cows. Translational Animal Science 2(suppl_1), S9-S13.
| Crossref | Google Scholar | PubMed |

Brasil (2000) Normative No. 3 for the technical regulation of stunning methods for humanitarian killing of slaughterhouse animals, January 17, 2000. Department of Agriculture, Brasilia, DF.

Carvalho JRR, Chizzotti ML, Schoonmaker JP, Teixeira PD, Lopes RC, Oliveira CVR, Ladeira MM (2016) Performance, carcass characteristics, and ruminal pH of Nellore and Angus young bulls fed a whole shelled corn diet. Journal of Animal Science 94, 2451-2459.
| Crossref | Google Scholar | PubMed |

Chaudhry S, Sidhu GPS (2022) Climate change regulated abiotic stress mechanisms in plants: a comprehensive review. Plant Cell Reports 41(1), 1-31.
| Crossref | Google Scholar | PubMed |

Choi SH, Gang GO, Sawyer JE, Johnson BJ, Kim KH, Choi CW, Smith B (2013) Fatty acid biosynthesis and lipogenic enzyme activities in subcutaneous adipose tissue of feedlot steers fed supplementary palm oil or soybean oil. Journal of Animal Science 91(5), 2091-2098.
| Crossref | Google Scholar | PubMed |

Choi SH, Park SK, Johnson BJ, Chung KY, Choi CW, Kim KH, Kim WY, Smith SB (2015) AMPKα, C/EBPβ, CPT1β, GPR43, PPARγ, and SCD Gene expression in single and co-cultured bovine satellite cells and intramuscular preadipocytes treated with palmitic, stearic, oleic, and linoleic acid. Asian-Australasian Journal of Animal Sciences 28, 411-419.
| Crossref | Google Scholar |

Cooke RF (2019) Early career achievement award: supplementing omega-6 fatty acids to enhance early embryonic development and pregnancy establishment in Bos indicus and B. taurus beef cows. Journal of Animal Science 97(1), 485-495.
| Crossref | Google Scholar | PubMed |

Cooke RF, Bohnert DW, Moriel P, Hess BW, Mills RR (2011) Effects of polyunsaturated fatty acid supplementation on ruminal in situ forage degradability, performance, and physiological responses of feeder cattle. Journal of Animal Science 89(11), 3677-3689.
| Crossref | Google Scholar | PubMed |

Cooke RF, Daigle CL, Moriel P, Smith SB, Tedeschi LO, Vendramini JMB (2020) Cattle adapted to tropical and subtropical environments: social, nutritional, and carcass quality considerations. Journal of Animal Science 98(2), skaa014.
| Crossref | Google Scholar |

Custodio L, Prados LF, Figueira DN, Yiannikouris A, Gloria EM, Holder VB, Pettigrew JE, Santin E, Resende FD, Siqueira GR (2020) Mycotoxin-contaminated diets and an adsorbent affect the performance of Nellore bulls finished in feedlots. Animal 14(10), 2074-2082.
| Crossref | Google Scholar | PubMed |

da Freiria LB, Zervoudakis JT, de Paula NF, Fonseca MA, da Rosa e Silva PIJL, Boas e Silva YRV, Possamai AJ (2022) Different fat sources in supplements for beef cattle at pasture. Tropical Animal Health and Production 54(3), 163.
| Crossref | Google Scholar | PubMed |

da Silva APV, Dias AM, Ítavo LCV, Ítavo CCBF, De Nadai Bonin Gomes M, Nogueira É, dos Santos Difante G, Gomes ENO, Junges L (2022) The influence of protected fat in supplements on the performance and carcass characteristics of Nellore beef bulls in tropical pasture. Tropical Animal Health and Production 54(2), 86.
| Crossref | Google Scholar |

De Paula NF, Paulino MF, Couto VRM, Detmann E, Maciel IFS, Barros LV, Lopes SA, Valente ÉEL, Zervoudakis JT, Martins LS (2019) Effects of supplementation plan on intake, digestibility, eating behavior, growth performance, and carcass characteristics of grazing beef cattle. Semina: Ciências Agrárias 40(6Supl2), 3233-3248.
| Crossref | Google Scholar |

De Vries MFW (1995) Estimating forage intake and quality in grazing cattle: a reconsideration of the hand-plucking method. Journal of Range Management 48(4), 370-375 Available at https://journals.uair.arizona.edu/index.php/jrm/article/download/9038/8650.
| Google Scholar |

Detmann E, Souza MA, Valadares Filho SC, Queiroz AC, Berchielli TT, Saliba EOS, Cabral LS, Pina DS, Ladeira MM, Azevedo JAG (Eds) (2012) ‘Métodos para 392 análise de alimentos.’ 1st edn. p. 214. (Visconde do Rio Branco: Suprema)

Detmann E, Valente ÉEL, Batista ED, Huhtanen P (2014) An evaluation of the performance and efficiency of nitrogen utilization in cattle fed tropical grass pastures with supplementation. Livestock Science 162, 141-153.
| Crossref | Google Scholar |

Gagaoua M, Monteils V, Couvreur S, Picard B (2017) Identification of biomarkers associated with the rearing practices, carcass characteristics, and beef quality: an integrative approach. Journal of Agricultural and Food Chemistry 65(37), 8264-8278.
| Crossref | Google Scholar | PubMed |

Habermann E, Contin DR, Afonso LF, Barosela JR, de Pinho Costa KA, Viciedo DO, Groppo M, Martinez CA (2022) Future warming will change the chemical composition and leaf blade structure of tropical C3 and C4 forage species depending on soil moisture levels. Science of The Total Environment 821, 153342.
| Crossref | Google Scholar | PubMed |

Hess BW, Moss GE, Rule DC (2008) A decade of developments in the area of fat supplementation research with beef cattle and sheep. Journal of Animal Science 86(suppl_14), E188-E204.
| Crossref | Google Scholar |

Huerta-Leidenz NO, Cross HR, Savell JW, Lunt DK, Baker JF, Smith SB (1996) Fatty acid composition of subcutaneous adipose tissue from male calves at different stages of growth. Journal Animal of Science 74(6), 1256-1264.
| Crossref | Google Scholar |

Hughes JM, Clarke FM, Purslow PP, Warner RD (2020) Meat color is determined not only by chromatic heme pigments but also by the physical structure and achromatic light scattering properties of the muscle. Comprehensive Reviews in Food Science and Food Safety 19(1), 44-63.
| Crossref | Google Scholar |

Irshad A, Kandeepan G, Kumar S, Ashish KA, Vishnuraj MR, Shukla V (2013) Factors influencing carcass composition of livestock: a review. Journal of Animal Production Advances 3(5), 177-186 Available at https://wwwresearchgatenet/publication/273716160.
| Google Scholar |

Jenkins TC (1993) Lipid metabolism in the rumen. Journal of Dairy Science 76(12), 3851-3863.
| Crossref | Google Scholar |

Mancini RA, Hunt MC (2005) Current research in meat color. Meat Science 71(1), 100-121.
| Crossref | Google Scholar |

Mangrum KS, Tuttle G, Duckett SK, Sell GS, Krehbiel CR, Long NM (2016) The effect of supplementing rumen undegradable unsaturated fatty acids on marbling in early-weaned steers. Journal of Animal Science 94(2), 833-844.
| Crossref | Google Scholar |

Martins TS, Sanglard LMP, Silva W, Chizzotti ML, Rennó LN, Serão NVL, Silva FF, Guimarães SEF, Ladeira MM, Dodson MV, Du M, Duarte MS (2015) Molecular factors underlying the deposition of intramuscular fat and collagen in skeletal muscle of Nellore and angus cattle. PLoS ONE 10(10), 1-13.
| Crossref | Google Scholar |

Mendes GA, Rocha Júnior VR, Ruas JRM, Silva FV, Caldeira LA, Pereira MEG, Soares FDS, Pires DAA (2012) Características de carcaça e qualidade da carne de novilhas alimentadas com silagem de capim-marandu. Pesquisa Agropecuária Brasileira 47(12), 1774-1781.
| Crossref | Google Scholar |

Nascimento FA, Silva NC, Prados LF, Pacheco RDL, Johnson BJ, Cappellozza BI, Resende FD, Siqueira GR (2020) Calcium salts of fatty acids with varying fatty acid profiles in diets of feedlot-finished Bos indicus bulls: impacts on intake, digestibility, performance, and carcass and meat characteristics. Journal of Animal Science 98(12), pskaa382.
| Crossref | Google Scholar |

Nave RLG, Sulc RM, Barker DJ (2013) Relationships of forage nutritive value to cool-season grass canopy characteristics. Crop Science 53(1), 341-348.
| Crossref | Google Scholar |

Owens FN, Dubeski P, Hanson CF (1993) Factors that alter the growth and development of ruminants. Journal of Animal Science 71(11), 3138-3150.
| Crossref | Google Scholar |

Owens FN, Gill DR, Secrist DS, Coleman SW (1995) Review of some aspects of growth and development of feedlot cattle. Journal of Animal Science 73(10), 3152-3172.
| Crossref | Google Scholar | PubMed |

Palma MNN, Reis WLS, Rodrigues JPP, Silva TE, Franco MO, Rennó LN, Detmann E (2023) Strategies of energy supplementation for cattle fed tropical forage and infrequently supplemented with protein. Animal Feed Science and Technology 297, 115599.
| Crossref | Google Scholar |

Palmquist DL, Jenkins TC (1980) Fat in lactation rations. Journal of Dairy Science 63(1), 1-14.
| Crossref | Google Scholar |

Paulino PVR, Oliveira TS, Gionbeli MP, Gallo SB (2013) Dietas sem forragem para terminação de animais ruminantes. Revista Científica de Produção Animal 15(2), 161-172.
| Crossref | Google Scholar |

Pflanzer SB, de Felício PE (2011) Moisture and fat content, marbling level and color of boneless rib cut from Nellore steers varying in maturity and fatness. Meat Science 87(1), 7-11.
| Crossref | Google Scholar |

Pikul J, Leszczynski DE, Kummerow FA (1989) Evaluation of three modified TBA methods for measuring lipid oxidation in chicken meat. Journal of Agricultural and Food Chemistry 37(5), 1309-1313.
| Crossref | Google Scholar |

Poppi DP, McLennan SR (1995) Protein and energy utilization by ruminants at pasture. Journal of Animal Science 73(1), 278-290.
| Crossref | Google Scholar |

Ramírez-Zamudio GD, da Cruz WFG, Schoonmaker JP, de Resende FD, Siqueira GR, Neto ORM, Gionbelli TRS, Teixeira PD, Rodrigues LM, Gionbelli MP, Ladeira MM (2022) Effect of rumen-protected fat on performance, carcass characteristics and beef quality of the progeny from Nellore cows fed by different planes of nutrition during gestation. Livestock Science 258, 104851.
| Crossref | Google Scholar |

Ramos JML, Vargas JAC, Diniz EAL, Lacerda NG, Mezzomo R, Alves KS, Oliveira LRS, de Loureiro JPB, da Silva PR, Gomes DI (2022) Carcass traits and meat characteristics of grazing Nellore cattle submitted to different supplementation strategies in the tropics. Tropical Animal Health and Production 54(6), 362.
| Crossref | Google Scholar | PubMed |

Rosa BL, Sampaio AAM, Henrique W, Oliveira EAd, Pivaro TM, Andrade ATd, Fernandes ARM (2013) Performance and carcass characteristics of Nellore young bulls fed different sources of oils, protected or not from rumen degradation. Revista Brasileira de Zootecnia 42, 109-116.
| Crossref | Google Scholar |

Silva FFd, Sá JFd, Schio AR, Ítavo LCV, Silva RR, Mateus RG (2009) Suplementação a pasto: disponibilidade e qualidade x níveis de suplementação x desempenho. Revista Brasileira de Zootecnia 38, 371-389.
| Crossref | Google Scholar |

Simioni TA, Torrecilhas JA, Messana JD, Granja-Salcedo YT, Vito ES, Lima ARC, Sanchez JMD, Reis RA, Berchielli TT (2021) Influence of growing-phase supplementation strategies on intake and performance of different beef cattle genotypes in finishing phase on pasture or feedlot. Livestock Science 251, 104653.
| Crossref | Google Scholar |

Sollenberger LE, Cherney DJR (1995) Evaluating forage production and quality. In ‘Forages: The science of grassland agriculture 2’. pp. 97–110. (Iowa State University Press)

Sousa LCO, Palma MNN, Franco MO, Detmann E (2022) Does frequency of protein supplementation affect performance of cattle under grazing in tropical pastures? Animal Feed Science and Technology 289, 115316.
| Crossref | Google Scholar |

Torrecilhas JA, San Vito E, Fiorentini G, De Souza Castagnino P, Simioni TA, Lage JF, Baldi F, Duarte JM, da Silva LG, Reis RA, Berchielli TT (2021) Effects of supplementation strategies during the growing phase on meat quality of beef cattle finished in different systems. Livestock Science 247, 104465.
| Crossref | Google Scholar |

Van Soest PJ (1994) ‘Nutritional ecology of the ruminant.’ 2nd edn. (Cornell University: Ithaca, United States)

Warren HE, Scollan ND, Nute GR, Hughes SI, Wood JD, Richardson RI (2008) Effects of breed and a concentrate or grass silage diet on beef quality in cattle of 3 ages. II: Meat stability and flavour. Meat Science 78(3), 270-278.
| Crossref | Google Scholar | PubMed |