Dietary chromium-methionine supplementation and broiler (22–43 days) responses during heat stress. 2 - Physiological variables, and heat shock protein 70 and insulin-like growth factor-1 gene expression
Felipe Santos Dalólio
A , Luiz Fernando Teixeira Albino B , Haniel Cedraz de Oliveira B , Alba Kyonara Barbosa Alves Tenorio Fireman C , Alvaro Burin Junior C , Marcos Busanello D , Nilton Rohloff Junior E , Guilherme Luis Silva Tesser E * and Ricardo Vianna Nunes E
A
B
C
D
E
Abstract
Dietary supplementation with trace mineral chromium (Cr) has been shown to enhance the physiological responses of broilers subjected to heat stress (HS), modulate gene expression, and improve performance.
This study aimed to evaluate the impact of chromium–methionine (CrMet) supplementation on growth performance, body temperatures, lymphoid organ weights, hormones, blood parameters, and the expression of heat-shock protein-70 (HSP-70) and insulin-like growth factor-1 (IGF-1) genes in broilers under HS conditions (33°C for 12 h/day).
In the first experiment, 336 22-day-old male broilers were randomly distributed into four blocks with six treatments (0, 0.10, 0.20, 0.40, 0.80, and 1.20 mg/kg CrMet) and eight replicates with seven birds per cage. These broilers were subjected to HS from 22 to 43 days of age. In the second experiment, 24 male broilers, in total, at 43 days of age, previously exposed to HS, were randomly distributed to the same six treatments from the first experiment, with four replicates. Breast samples were collected for the analysis of HSP-70 and IGF-1 expression.
A quadratic effect (P < 0.05) was observed on bodyweight gain (BWG) and feed conversion ratio (FCR). The supplementation of 0.71 and 0.68 mg/kg improved BWG and FCR, respectively. At 28 days of age, cloacal and mean body temperatures, corticosterone, and thyroid hormones were quadratically affected (P < 0.05), while at 43 days of age, a linear effect (P < 0.05) was observed on haemoglobin concentration. There was a reduction (P < 0.05) in the expression of HSP-70 and an increase in IGF-1 (P < 0.05) in the breast tissue of broilers supplemented with CrMet.
The supplementation with 0.71 mg/kg and 0.68 mg/kg of CrMet improved BWG and FCR, respectively. Additionally, the supplementation with 0.80 mg/kg improved hormones, reduced HSP-70 and increased the expression of IGF-1 in broilers during HS.
These findings suggest that CrMet can be included in the diet of broiler chickens subjected to HS to enhance physiological responses and performance.
Keywords: animal physiology, animal production, growth performance, heat stress, hormones, mineral nutrition, organic mineral, trace mineral.
References
Akdemir F, Sahin N, Orhan C, Tuzcu M, Sahin K, Hayirli A (2015) Chromium–histidinate ameliorates productivity in heat-stressed Japanese quails through reducing oxidative stress and inhibiting heat-shock protein expression. British Poultry Science 56, 247-254.
| Crossref | Google Scholar | PubMed |
Almeida VV, Berenchtein B, Costa LB, Tse MLP, Braz DB, Miyada VS (2010) Ractopamine, chromium-methionine and their combinations as metabolism modifier feed additives of growing and finishing pigs. Brazilian Journal of Animal Science 39, 1969-1977.
| Crossref | Google Scholar |
Altan Ö, Pabuçcuoğlu A, Altan A, Konyalioğlu S, Bayraktar H (2003) Effect of heat stress on oxidative stress, lipid peroxidation, and some stress parameters in broilers. British Poultry Science 44, 545-550.
| Crossref | Google Scholar | PubMed |
Anderson RA (1997) Chromium as an essential nutrient for humans. Regulatory Toxicology and Pharmacology 26, S35-S41.
| Crossref | Google Scholar | PubMed |
Anderson RA, Allen J (1994) Nutrition of macrominerals and trace elements. In ‘Functional foods: designer foods, pharmafoods, nutraceuticals’. (Ed. I Goldberg), pp. 323–354. (Springer: Boston, MA, USA) doi:10.1007/978-1-4615-2073-3_15
Arif H, Hussain I, Mahmood MA, Abd El-Hack ME, Swelum AA, Alagawany M, Mahmoud AH, Ebaid H, Komany A (2019) Effect of varying levels of chromium propionate on growth performance and blood biochemistry of broilers. Animals 9, 935.
| Crossref | Google Scholar | PubMed |
Bahrami A, Moeini MM, Ghazi SH, Targhibi MR (2012) The effect of different levels of organic and inorganic chromium supplementation on immune function of broiler chicken under heat-stress conditions. Journal of Applied Poultry Research 21, 209-215.
| Crossref | Google Scholar |
Borges SA, Maiorka A, Silva AVF (2003) Heat stress physiology and electrolytes for broilers. Ciência Rural 33, 975-981.
| Crossref | Google Scholar |
Cedraz H, Gromboni JGG, Garcia Junior AAP, Farias Filho RV, Souza TM, Oliveira ER, Oliveira EB, Nascimento CS, Meneghetti C, Wenceslau AA (2017) Heat stress induces expression of HSP genes in genetically divergent chickens. PLoS ONE 12, e0186083.
| Crossref | Google Scholar | PubMed |
Cobb-Vantress (2013) ‘Broiler Management Guide.’ (Cobb-Vantress Inc.: Siloam Springs, AR, USA). Available at https://www.cob-vantress.com/
Cruzat VF, Donato Júnior J, Tirapegui J, Schneider CD (2008) Hormônio do crescimento e exercício físico: considerações atuais. Brazilian Journal of Pharmaceutical Sciences 44, 549-562.
| Crossref | Google Scholar |
Daher R, Yazbeck T, Jaoude JB, Abboud B (2009) Consequences of dysthyroidism on the digestive tract and viscera. World Journal of Gastroenterology 15, 2834-2838.
| Crossref | Google Scholar | PubMed |
Dalólio FS, Albino LFT, Silva JN, Campos PHRF, Lima HJ, Moreira J, Ribeiro Júnior V (2018) Dietary chromium supplementation for heat-stressed broilers. World’s Poultry Science Journal 74, 101-116.
| Crossref | Google Scholar |
Dalólio FS, Albino LFT, Silva JN, Fireman AKAT, Burin Júnior AM, Busanello M, Ribeiro Júnior V (2021) Dietary chromium-methionine supplementation and broiler (22–43 days) responses during heat stress. 1. Growth performance and carcass yield, metabolisable energy and serum biochemistry. Animal Production Science 61, 586-595.
| Crossref | Google Scholar |
Das R, Sailo L, Verma N, Bharti P, Saikia J, Imtiwati, Kumar R (2016) Impact of heat stress on health and performance of dairy animals: a review. Veterinary World 9, 260-268.
| Crossref | Google Scholar | PubMed |
Del Vesco AP, Gasparino E, Oliveira Neto AR, Guimarães SEF, Marcato SMM, Voltolini DM (2013) Dietary methionine effects on IGF-I and GHR mRNA expression in broilers. Genetics and Molecular Research 12, 6414-6423.
| Crossref | Google Scholar | PubMed |
Del Vesco AP, Gasparino E, Grieser DO, Zancanela V, Voltolini DM, Khatlab AS, Guimarães SE, Soares MA, Oliveira Neto AR (2015) Effects of methionine supplementation on the expression of protein deposition-related genes in acute heat stress-exposed broilers. PLoS ONE 25, e0115821.
| Crossref | Google Scholar |
Ebrahimzadeh SK, Farhoomand P, Noori K (2012) Immune response of broiler chickens fed diets supplemented with different levels of chromium methionine under heat stress conditions. Asian-Australasian Journal of Animal Science 25, 256-260.
| Crossref | Google Scholar |
Ezzat W, Abdallah EA, Rizk AM, Ouda MMM, Abd El-Krim RE (2017) Impact of chromium picolinate supplementation on productive performance, immune response, and heat shock proteins of broiler chickens under heat-stress condition. Egypt Poultry Science Journal 37, 559-583.
| Google Scholar |
Fu WJ, Hu J, Spencer T, Carroll R, Wu G (2006) Statistical models in assessing fold change of gene expression in real-time RT-PCR experiments. Computational Biology and Chemistry 30, 21-26.
| Crossref | Google Scholar | PubMed |
Ghazi SH, Habibian M, Moeini MM, Abdolmohammadi AR (2012) Effects of different levels of organic and inorganic chromium on growth performance and immunocompetence of broilers under heat stress. Biological Trace Element Research 146, 309-317.
| Crossref | Google Scholar | PubMed |
Giloh M, Shinder D, Yahav S (2012) Skin surface temperature of broiler chickens is correlated to body core temperature and is indicative of their thermoregulatory status. Poultry Science 91, 175-188.
| Crossref | Google Scholar | PubMed |
Godini A, Ghasemi A, Zahediasl S (2015) The possible mechanisms of the impaired insulin secretion in hypothyroid rats. PLoS ONE 10, e0131198.
| Crossref | Google Scholar | PubMed |
Gupta SC, Siddique HR, Mathur N, Vishwakarma AL, Mishra RK, Saxena DK, Chowdhuri DK (2007) Introduction of Hsp70, alteration in oxidative stress markers, and apoptosis against dichlorvos exposure in transgenic Drosophila melanogaster: modulation by reactive oxygen species. Biochimica et Biophysica Acta 1770, 1382-1394.
| Crossref | Google Scholar | PubMed |
Hayat K, Bodinga BM, Han D, Yang X, Sun Q, Aleya L, Abdel-Daim MM, Yang X (2020) Effects of dietary inclusion of chromium propionate on growth performance, intestinal health, immune response, and nutrient transporter gene expression in broilers. Science of The Total Environment 705, 135869.
| Crossref | Google Scholar | PubMed |
He SP, Arowolo MA, Medrano RF, Li S, Yu QF, Chen JY, He JH (2018) Impact of heat stress and nutritional interventions on poultry production. World’s Poultry Science Journal 74, 647-664.
| Crossref | Google Scholar |
Jahanian R, Rasouli E (2015) Dietary chromium methionine supplementation could alleviate immunosuppressive effects of heat stress in broiler chicks. Journal of Animal Science 93, 3355-3363.
| Crossref | Google Scholar | PubMed |
Kumari KNR, Nath DN (2018) Ameliorative measures to counter heat stress in poultry. World’s Poultry Science Journal 74, 117-130.
| Crossref | Google Scholar |
Li R, Zhou Y, Li Y, Guo L, Zhang Y, Qi Z (2018) Effects of chromium picolinate supplementation on growth performance, small intestine morphology, and antioxidant status in ducks under heat stress conditions. International Journal of Morphology 36, 226-234.
| Crossref | Google Scholar |
Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25, 402-408.
| Crossref | Google Scholar | PubMed |
Lu L, Zhao LL, Dong SY, Liao XD, Dong XY, Zhang LY, Luo XG (2019) Dietary supplementation of organic or inorganic chromium modulates the immune responses of broilers vaccinated with avian influenza virus vaccine. Animal 13, 983-991.
| Crossref | Google Scholar | PubMed |
Martinez B, Ortiz RM (2017) Thyroid hormone regulation and insulin resistance: insights from animals naturally adapted to fasting. Physiology 32, 141-151.
| Crossref | Google Scholar | PubMed |
Norain TM, Ismail IB, Abdoun KA, Al-Haidary AA (2013) Dietary inclusion of chromium to improve growth performance and immune-competence of broilers under heat stress. Italian Journal of Animal Science 12, 562-566.
| Crossref | Google Scholar |
NRC (1997) ‘The role of chromium in animal nutrition.’ (National Academies Press: Washington, DC, USA) doi:10.17226/5778
Orhan C, Akdemir F, Sahin N, Tuzcu M, Komorowski JR, Hayirli A, Sahin K (2012) Chromium histidinate protects against heat stress by modulating the expression of hepatic nuclear transcription factors in quail. British Poultry Science 53, 828-835.
| Crossref | Google Scholar | PubMed |
Phalen DN, Mitchell ME, Cavazos-Martinez ML (1996) Evaluation of three heat sources for their ability to maintain core body temperature in the anesthetized avian patient. Journal of Avian Medicine and Surgery 10, 174-178 Available at http://www.jstor.org/stable/30133092.
| Google Scholar |
Rajkumar U, Vinoth A, Reddy EPK, Shanmugan M, Rao SVR (2018) Effect of supplemental trace minerals on HSP70 mRNA expression in commercial broiler chickens. Animal Biotechnology 29, 20-25.
| Crossref | Google Scholar |
Rao SVR, Raju MVLN, Panda AK, Poonam NS, Murthy OK, Sunder GS (2012) Effect of dietary supplementation of organic chromium on performance, carcass traits, oxidative parameters, and immune responses in commercial broiler chickens. Biological Trace Element Research 147, 135-141.
| Crossref | Google Scholar | PubMed |
Rao SVR, Prakash B, Raju MVLN, Panda AK, Kumari RK, Reddy EPK (2016) Effect of supplementing organic forms of zinc, selenium, and chromium on performance, antioxidant, and immune responses in broiler chickens reared in tropical summer. Biological Trace Element Research 172, 511-520.
| Crossref | Google Scholar | PubMed |
Richards SA (1971) The significance of changes in the temperature of the skin and body core of the chicken in the regulation of heat loss. Journal of Physiology 216, 1-10.
| Crossref | Google Scholar | PubMed |
Roll VFB, Lopes LL, Rossi P, Anciuti MA, Rutz F, Xavier EG, Silva SS (2010) Hematology of broilers fed diets containing aflatoxins and mycotoxin adsorbent. Archivos de Zootecnia 59, 93-101.
| Crossref | Google Scholar |
Ryu ST, Park BS, Bang HT, Kang HK, Hwangbo J (2016) Effects of anti-heat diet and inverse lighting on growth performance, immune organ, microorganism, and short-chain fatty acids of broiler chickens under heat stress. Journal of Environmental Biology 37, 185-192.
| Google Scholar | PubMed |
Sacheck JM, Ohtsuka A, McLary SC, Goldberg AL (2004) IGF-I stimulates muscle growth by suppressing protein breakdown and expression of atrophy-related ubiquitin ligases, atrogin-1 and MuRF1. American Journal of Physiology - Endocrinology and Metabolism 287, E591-E601.
| Crossref | Google Scholar | PubMed |
Safwat AM, Elnaggar AS, Elghalid OA, EL-Tahawy WS (2020) Effects of different sources and levels of dietary chromium supplementation on performance of broiler chicks. Animal Science Journal 91, e13448.
| Crossref | Google Scholar | PubMed |
Sahin K, Sahin N, Onderic M, Gursu F, Cikim G (2002) Optimal dietary concentration of chromium for alleviating the effect of heat stress on growth, carcass qualities, and some serum metabolites of broiler chickens. Biological Trace Element Research 89, 53-64.
| Crossref | Google Scholar | PubMed |
Sahin K, Sahin N, Küçük O (2003) Effects of chromium and ascorbic acid supplementation on growth, carcass traits, serum metabolites, and antioxidant status of broiler chickens reared at a high environmental temperature (32°C). Nutrition Research 23, 225-238.
| Crossref | Google Scholar |
Sahin N, Akdemir F, Tuzcu M, Hayrli A, Smith MO, Sahin K (2010) Effects of supplemental chromium sources and levels on performance, lipid peroxidation, and proinflammatory markers in heat-stressed quails. Animal Feed Science and Technology 159, 143-149.
| Crossref | Google Scholar |
Sahin N, Hayrli A, Orhan C, Tuzcu M, Akdemir F, Komorowski JR, Sahin K (2017) Effects of supplemental chromium form on performance and oxidative stress in broilers exposed to heat stress. Poultry Science 96, 4317-4324.
| Crossref | Google Scholar | PubMed |
Sahin N, Hayirli A, Orhan C, Tuzcu M, Komorowski JR, Sahin K (2018) Effects of the supplemental chromium form on performance and metabolic profile in laying hens exposed to heat stress. Poultry Science 97, 1298-1305.
| Crossref | Google Scholar | PubMed |
Saleh AA, Ragab MM, Ahmed EAM, Abudabos AM, Ebeid TA (2018) Effect of dietary zinc–methionine supplementation on growth performance, nutrient utilization, antioxidative properties and immune response in broiler chickens under high ambient temperature. Journal of Applied Animal Research 46, 820-827.
| Crossref | Google Scholar |
SAS Institute, Inc. (2012) SAS OnDemand for Academics. Release 9.04.01M5P09132017. SAS Institute Inc., Cary, NC, USA. Available at hhttps://odamid.oda.sas.com/SASStudio/
Siegel HS (1995) Stress, strains and resistance. British Poultry Science 36, 3-22.
| Crossref | Google Scholar | PubMed |
Silva SRG, Abreu MLT, Lopes JB, Leal DIB, Almendra SNO, Silva SMMS, Costa SEM (2014) Desempenho e resposta imune de frangos de corte alimentados com dietas suplementadas com cromo na forma orgânica. Revista Brasileira de Medicina Veterinária 21, 199-203.
| Google Scholar |
Steibel JP, Poletto R, Coussens PM, Rosa GJM (2009) A powerful and flexible linear mixed model framework for the analysis of relative quantification RT-PCR data. Genomics 94, 146-152.
| Crossref | Google Scholar | PubMed |
Valente Junior DT, Barbosa LMR, Soares MH, Rodrigues GA, Gomes M, Silva CB, Teixeira LM, Cunha Júnior RL, Abranches FF, Saraiva A (2021) Dietary supplementation of chromium for finishing pigs. Ciência Rural 51, e20200554.
| Crossref | Google Scholar |
Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F (2002) Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biology 3, 1-12.
| Crossref | Google Scholar |
Vignale K, Caldas JV, England JA, Boonsinchai N, Magnuson A, Pollock ED, Dridi S, Owens CM, Coon CN (2017) Effect of white striping myopathy on breast muscle (Pectoralis major) protein turnover and gene expression in broilers. Poultry Science 96, 886-893.
| Crossref | Google Scholar | PubMed |
Vincent JB (2000) The biochemistry of chromium. The Journal of Nutrition 130, 715-718.
| Crossref | Google Scholar | PubMed |
Vincent JB (2001) The bioinorganic chemistry of chromium (III). Polyhedron 20, 1-26.
| Crossref | Google Scholar |
Vincent JB (2010) Chromium: celebrating 50 years as an essential element? Dalton Transactions 39, 3787-3794.
| Crossref | Google Scholar | PubMed |
Vincent JB (2017) New evidence against Chromium as an essential trace element. The Journal of Nutrition 147, 2212-2219.
| Crossref | Google Scholar | PubMed |
Wasti S, Sah N, Mishra B (2020) Impact of heat stress on poultry health and performances, and potential mitigation strategies. Animals 10, 1266.
| Google Scholar |
Wen C, Jiang X, Ding L, Wang T, Zhou Y (2017) Effects of dietary methionine on breast muscle growth, myogenic gene expression and IGF-I signaling in fast- and slow-growing broilers. Scientific Reports 7, 1924.
| Crossref | Google Scholar |
Williams CH, David DJ, Iismaa O (1962) The determination of chromic oxide in faeces samples by atomic absorption spectrophotometry. Journal of Agricultural Science 59, 381-385.
| Crossref | Google Scholar |
Youssef IMI, Abdo IMI, Elsukkary HFA, El-Kady MF, Elsayed M (2022) Effects of dietary supplementation of chromium methionine chelate on growth performance, oxidative stress, hematological indices, and carcass traits of broiler chickens. Tropical Animal Health and Production 54, 267.
| Crossref | Google Scholar | PubMed |
Zheng C, Huang Y, Xiao F, Lin X, Lloyd K (2016) Effects of supplemental chromium source and concentration on growth, carcass characteristics, and serum lipid parameters of broilers reared under normal conditions. Biological Trace Element Research 169, 352-358.
| Crossref | Google Scholar | PubMed |
