Investigating the role of blow fly olfaction in flystrike in sheep

Guanjie Yan; Anthony C. Schlink; Shimin Liu; Johan C. Greeff; Gavin R. Flematti; Graeme B. Martin

doi:10.1071/AN23238

PERSPECTIVES ON ANIMAL BIOSCIENCES (Open Access)

Previous Contents Vol 64(2)

Investigating the role of blow fly olfaction in flystrike in sheep

Guanjie Yan

^A ^B ^C , Anthony C. Schlink ^A , Shimin Liu

^A ^C , Johan C. Greeff

^A ^D , Gavin R. Flematti

^E and Graeme B. Martin

^A ^C ^*

+ Author Affiliations

- Author Affiliations

^A UWA Institute of Agriculture, The University of Western Australia, Perth, WA 6009, Australia.

^B China-UK-NYNU-RRes Joint Laboratory of Insect Biology, Nanyang Normal University, Nanyang, PR China.

^C UWA School of Agriculture and Environment, The University of Western Australia, Perth, WA 6009, Australia.

^D Department of Primary Industries and Regional Development, Perth, 3 Baron Hay Court, South Perth, WA 6151, Australia.

^E School of Molecular Sciences, The University of Western Australia, Perth, WA 6009, Australia.

^* Correspondence to: graeme.martin@uwa.edu.au

Handling Editor: Wayne Bryden

Animal Production Science 64, AN23238 https://doi.org/10.1071/AN23238

Submitted: 6 July 2023 Accepted: 3 December 2023 Published: 5 January 2024

© 2024 The Author(s) (or their employer(s)). Published by CSIRO Publishing. This is an open access article distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND)

Abstract

Breech flystrike is a painful, debilitating and potentially lethal disease caused by the larvae of the blowfly, Lucilia cuprina, and, despite many years of research, it remains a serious financial and animal-welfare issue for the Merino sheep industry in Australia. The common methods of prevention, namely insecticides, crutching and ‘mulesing’, are problematical, so alternative approaches are needed. Breeding for resistance to breech strike is a fundamentally attractive proposition, but the trait itself is difficult and expensive to quantify in large numbers of sheep in extensive production systems. Several indirect traits are correlated with susceptibility to flystrike, but a large proportion of the variation in susceptibility remains unexplained. The common thread through those indirect traits is odour, so we turned to the biology of insect olfaction and its role in fly–sheep interactions. L. cuprina uses odours to detect and locate potential hosts over long distances, to guide orientation and landing behaviour, and to select egg-laying sites. Preliminary studies demonstrated the importance of confining our work to gravid female L. cuprina, and also validated the use of flies reared in the laboratory for experimentation. Using laboratory-reared flies and a combination of gas chromatography–mass spectrometry with electroantennographic detection, we identified odouriferous compounds from sheep that the antenna of L. cuprina can detect. To determine whether the identified compounds were attractive or repulsive, we needed to use a behaviour test. In preliminary studies, we compared four behaviour bioassays (Y-tube, landing time, visiting frequency, and trap) and found the trap to be the most effective. We observed that L. cuprina was attracted by several compounds in Merino wool, including octanal, nonanal and dimethyl trisulfide. We also found that the wool levels of octanal and nonanal are heritable in Merino sheep, suggesting that these compounds might be useful as traits in selection for flystrike resistance. Another possibility is that these olfactory-active compounds might guide efforts to modify the genome of sheep, or perhaps even L. cuprina. Success in these endeavours could save as much as A$200 m per year for the Australian Merino-based industries, while also improving the image of wool in world markets.

Keywords: antenna, behaviour, blow fly, breeding, electroantennography, flystrike, Lucilia cuprina, Merino.

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