Sheep grazing Trigonella balansae had productivity, health and meat quality similar to sheep grazing subterranean clover or French serradella
Matt G. Wilmot A , Hayley C. Norman A * , Joshua Hendry A , Paul Young A , Elizabeth Hulm A , Andrew Toovey A , Jane Speijers B and Robert Harrison CA CSIRO Agriculture and Food, 147 Underwood Avenue, Floreat, WA 6014, Australia.
B Speijers Consulting, 11a Swanbourne Street, Fremantle, WA 6160, Australia.
C Department of Primary Industries and Regional Development and Murdoch University, 3 Baron-Hay Court, South Perth, WA 6151, Australia.
Animal Production Science 63(2) 152-167 https://doi.org/10.1071/AN22287
Submitted: 28 July 2022 Accepted: 24 November 2022 Published: 5 January 2023
© 2023 The Author(s) (or their employer(s)). Published by CSIRO Publishing. This is an open access article distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND)
Abstract
Context: An accession of the annual legume Trigonella balansae Boiss. & Reuter. has been selected for commercial release in Australia.
Aims: As part of a duty-of-care assessment, we tested the hypothesis that sheep grazing the trigonella accession will have liveweight, condition scores and wool production similar to those of sheep grazing two widely adopted annual legumes, subterranean clover (subclover, Trifolium subterraneum L. cv. Dalkeith) and French serradella (Ornithopus sativus Brot. cv. Erica). We also hypothesised that sheep grazing the three legumes will have similar meat quality and health, as indicated by blood plasma panel analysis.
Methods: Wethers (n = 6) grazed plots (n = 4) of three pasture species. Liveweight, condition scores and wool growth were measured, and a subset of animals were subject to blood sampling to assess health. Consumer sensory taste analysis was used to assess meat eating quality from two sheep per plot. Forage biomass, dry-matter digestibility, crude protein, fibre, mineral content and isoflavones were measured across the plant’s lifecycle.
Key results: There were no significant differences in liveweight, wool growth, wool yield or condition score that were associated with pasture species during the grazing period. There were differences in dry-matter digestibility, minerals and crude protein content of the pastures over time. As trigonella matured, it did not meet the minimum sulfur, phosphorus, manganese, molybdenum and copper requirements for mature sheep. We found some differences in indications of animal health; however, there were no significant systematic detrimental differences between sheep grazing trigonella and the other two species. In total, 2 of 24 individual sheep grazing trigonella had more than two plasma indicators of liver damage. There were no differences in meat tenderness, juiciness, flavour and overall consumer acceptability.
Conclusions: The data supported the hypotheses. Under the conditions of this experiment, the pasture legumes had the same relative feeding value. Some care must be taken in interpreting the outcome as the trigonellacomprised only 18% of the dry matter on offer at the termination of grazing.
Implications: Results of this study will be considered during the commercialisation process for T. balansae.
Keywords: forage quality, grassland management, ley farming system, meat flavour, pasture improvement, plant secondary compounds, predicted growth, ruminant modelling, sward composition.
References
Adams NR (1998) Clover phyto-oestrogens in sheep in Western Australia. Pure and Applied Chemistry 70, 1855–1862.| Clover phyto-oestrogens in sheep in Western Australia.Crossref | GoogleScholarGoogle Scholar |
Agustini K, Sriningsih S, Effe J (2015) Acute toxicity study of ethanolic extract of fenugreek seeds (Trigonella foenum-graecum L.) on white rats. Journal of Indonesian Medicinal Plants 8, 9–13.
Bennetts HW, Underwood EJ, Shier FL (1946) A specific breeding problem of sheep on subterranean clover pastures in Western Australia. Australian Veterinary Journal 22, 2–12.
| A specific breeding problem of sheep on subterranean clover pastures in Western Australia.Crossref | GoogleScholarGoogle Scholar |
Cramer DA, Barton RA, Shorland FB, Czochanska Z (1967) A comparison of the effects of white clover (Trifolium repens) and of perennial ryegrass (Lolium perenne) on fat composition and flavour of lamb. The Journal of Agricultural Science 69, 367–373.
| A comparison of the effects of white clover (Trifolium repens) and of perennial ryegrass (Lolium perenne) on fat composition and flavour of lamb.Crossref | GoogleScholarGoogle Scholar |
Czochanska Z, Shorland FB, Barton RA, Rae AL (1970) A note on the effect of the length of the resting period before slaughter on the intensity of flavour and odour of lamb. New Zealand Journal of Agricultural Research 13, 662–663.
| A note on the effect of the length of the resting period before slaughter on the intensity of flavour and odour of lamb.Crossref | GoogleScholarGoogle Scholar |
de Vega A, Poppi DP (1997) Extent of digestion and rumen condition as factors affecting passage of liquid and digesta particles in sheep. The Journal of Agricultural Science 128, 207–215.
| Extent of digestion and rumen condition as factors affecting passage of liquid and digesta particles in sheep.Crossref | GoogleScholarGoogle Scholar |
de Koning CT, Beale PE, Duncan AJ, Hughes S, Haskard K (2001) A technique for assessing seed survival of new pasture legumes following grazing by sheep. In ‘Proceedings of the XIX international grasslands congress’, San Paulo Brazil, 11–21 February 2021. Published Online. (Fundacao de Estudos Agrarios Luiz de Queiroz)
Fortune JA, Cocks PS, Macfarlane CK, Smith FP (1995) Distribution and abundance of annual legume seeds in the wheatbelt of Western Australia. Australian Journal of Experimental Agriculture 35, 189–197.
| Distribution and abundance of annual legume seeds in the wheatbelt of Western Australia.Crossref | GoogleScholarGoogle Scholar |
Freer M, Moore AD, Donnelly JR (1997) GRAZPLAN: Decision support systems for Australian grazing enterprises: II. The animal biology model for feed intake, production and reproduction and the Grazfeed DSS. Agricultural Systems 54, 77–126.
| GRAZPLAN: Decision support systems for Australian grazing enterprises: II. The animal biology model for feed intake, production and reproduction and the Grazfeed DSS.Crossref | GoogleScholarGoogle Scholar |
Freer M, Dove H, Nolan JV (2007) ‘Nutrient requirements of domesticated ruminants.’ (CSIRO Publishing: Melbourne)
Frye EA, Behling-Kelly EL, Lejuene M, Webb JL (2022) Complete blood count and biochemistry reference intervals for healthy adult sheep in the northeastern United States. Veterinary Clinical Pathology 51, 119–125.
| Complete blood count and biochemistry reference intervals for healthy adult sheep in the northeastern United States.Crossref | GoogleScholarGoogle Scholar |
Hackney B, Rodham C, Piltz J (2013) ‘Using French serradella to increase crop and livestock production.’ (Meat & Livestock Australia: Sydney, NSW, Australia) Available at http://www.mla.com.au/News-and-resources/Publication-details?pubid=6124 [Verified 2 September 2020]
Harrison RJ, Howieson JG, Yates RJ, Nutt BJ (2021) Long-term storage of forage legumes greatly alters the hard seed breakdown pattern in situ. Grass and Forage Science 76, 72–81.
| Long-term storage of forage legumes greatly alters the hard seed breakdown pattern in situ.Crossref | GoogleScholarGoogle Scholar |
Hassan WM (2014) Influence of fenugreek seeds (Trigonella foenum graecum) on blood parameters, kidney, liver and mammary gland function for parturited Aissi ewes. Al-Qadisiya Journal for Agricultural Sciences 4, 1–12.
| Influence of fenugreek seeds (Trigonella foenum graecum) on blood parameters, kidney, liver and mammary gland function for parturited Aissi ewes.Crossref | GoogleScholarGoogle Scholar |
Howie JH, Ballard RA, de Koning C, Sandral G, Charman N (2001). Trigonella balansae – a new pasture legume for the alkaline soils of southern Australia? In ‘Proceedings of the 10th Australian agronomy conference’, Hobart. (Australian Society of Agronomy). Published online. Available at https://scholar.google.com/scholar?q=Trigonella%20balansaea%20new%20pasture%20legume%20for%20the%20alkaline%20soils%20of%20southern%20Australia.
Howieson JG, O’Hara GW, Carr SJ (2000) Changing roles for legumes in Mediterranean agriculture: developments from an Australian perspective. Field Crops Research 65, 107–122.
| Changing roles for legumes in Mediterranean agriculture: developments from an Australian perspective.Crossref | GoogleScholarGoogle Scholar |
Jones ML, Allison RW (2007) Evaluation of the ruminant complete blood cell count. Veterinary Clinics of North America: Food Animal Practice 23, 377–402.
| Evaluation of the ruminant complete blood cell count.Crossref | GoogleScholarGoogle Scholar |
Langlands JP, Wheeler JL (1968) The dyebanding and tattooed patch procedures for estimating wool production and obtaining samples for the measurement of fibre diameter. Australian Journal of Experimental Agriculture and Animal Husbandry 8, 265–269.
| The dyebanding and tattooed patch procedures for estimating wool production and obtaining samples for the measurement of fibre diameter.Crossref | GoogleScholarGoogle Scholar |
Loi A, Howieson JG, Nutt BJ, Carr SJ (2005) A second generation of annual pasture legumes and their potential for inclusion in Mediterranean-type farming systems. Australian Journal of Experimental Agriculture 45, 289–299.
| A second generation of annual pasture legumes and their potential for inclusion in Mediterranean-type farming systems.Crossref | GoogleScholarGoogle Scholar |
Loi A, Nutt BJ, Howieson JG, Yates RJ, Norman HC (2012) Preliminary assessment of bladder clover (Trifolium spumosum L.) as an annual legume for ley farming systems in southern Australia. Crop & Pasture Science 63, 582–591.
| Preliminary assessment of bladder clover (Trifolium spumosum L.) as an annual legume for ley farming systems in southern Australia.Crossref | GoogleScholarGoogle Scholar |
Mannetje L, Haydock KP (1963) The dry-weight-rank method for the botanical analysis of pasture. Grass and Forage Science 18, 268–275.
| The dry-weight-rank method for the botanical analysis of pasture.Crossref | GoogleScholarGoogle Scholar |
Masters DG, Mata G, Revell CK, Davidson RH, Norman HC, Nutt BJ, Solah V (2006) Effects of Prima gland clover (Trifolium glanduliferum Boiss cv. Prima) consumption on sheep production and meat quality. Australian Journal of Experimental Agriculture 46, 291–297.
| Effects of Prima gland clover (Trifolium glanduliferum Boiss cv. Prima) consumption on sheep production and meat quality.Crossref | GoogleScholarGoogle Scholar |
Masters DG, Norman HC, Thomas DT (2019) Minerals in pastures – are we meeting the needs of livestock? Crop & Pasture Science 70, 1184–1195.
| Minerals in pastures – are we meeting the needs of livestock?Crossref | GoogleScholarGoogle Scholar |
Nair RM, Dundas IS, Wallwork M, Verlin DC, Waterhouse L, Dowling K (2004) Breeding System in a population of Trigonella balansae (Leguminosae). Annals of Botany 94, 883–888.
| Breeding System in a population of Trigonella balansae (Leguminosae).Crossref | GoogleScholarGoogle Scholar |
Nichols PGH, Loi A, Nutt BJ, Evans PM, Craig AD, Pengelly BC, Dear BS, Lloyd DL, Revell CK, Nair RM, Ewing MA, Howieson JG, Auricht GA, Howie JH, Sandral GA, Carr SJ, de Koning CT, Hackney BF, Crocker GJ, Snowball R, Hughes SJ, Hall EJ, Foster KJ, Skinner PW, Barbetti MJ, You MP (2007) New annual and short-lived perennial pasture legumes for Australian agriculture – 15 years of revolution. Field Crops Research 104, 10–23.
| New annual and short-lived perennial pasture legumes for Australian agriculture – 15 years of revolution.Crossref | GoogleScholarGoogle Scholar |
Nichols PGH, Foster KJ, Piano E, Pecetti L, Kaur P, Ghamkhar K, Collins WJ (2013) Genetic improvement of subterranean clover (Trifolium subterraneum L.). 1. Germplasm, traits and future prospects. Crop & Pasture Science 64, 312–346.
| Genetic improvement of subterranean clover (Trifolium subterraneum L.). 1. Germplasm, traits and future prospects.Crossref | GoogleScholarGoogle Scholar |
Norman HC, Masters DG, Rintoul AJ, Wilmot MG, Jayasena V, Loi A, Revell CK (2005) The relative feeding value of a new pasture legume, eastern star clover (Trifolium dasyurum), compared with subterranean clover (Trifolium subterraneum. Australian Journal of Agricultural Research 56, 637–644.
| The relative feeding value of a new pasture legume, eastern star clover (Trifolium dasyurum), compared with subterranean clover (Trifolium subterraneum.Crossref | GoogleScholarGoogle Scholar |
Norman HC, Loi A, Wilmot MG, Rintoul AJ, Nutt BJ, Revell CK (2013) Sheep grazing bladder clover (Trifolium spumosum L.) had similar productivity and meat quality to sheep grazing subterranean clover (Trifolium subterraneum L.). Animal Production Science 53, 209–216.
| Sheep grazing bladder clover (Trifolium spumosum L.) had similar productivity and meat quality to sheep grazing subterranean clover (Trifolium subterraneum L.).Crossref | GoogleScholarGoogle Scholar |
Norman HC, Hulm E, Humphries AW, Hughes SJ, Vercoe PE (2020) Broad near-infrared spectroscopy calibrations can predict the nutritional value of >100 forage species within the Australian feedbase. Animal Production Science 60, 1111–1122.
| Broad near-infrared spectroscopy calibrations can predict the nutritional value of >100 forage species within the Australian feedbase.Crossref | GoogleScholarGoogle Scholar |
Nutt BJ, Loi A, Hackney B, Yates RJ, D’Antuono M, Harrison RJ, Howieson JG (2021) ‘Summer sowing’: a successful innovation to increase the adoption of key species of annual forage legumes for agriculture in Mediterranean and Temperate environments. Grass and Forage Science 76, 93–104.
| ‘Summer sowing’: a successful innovation to increase the adoption of key species of annual forage legumes for agriculture in Mediterranean and Temperate environments.Crossref | GoogleScholarGoogle Scholar |
O’Fallon JV, Busboom JR, Nelson ML, Gaskins CT (2007) A direct method for fatty acid methyl ester synthesis: application to wet meat tissues, oils, and feedstuffs. Journal of Animal Science 85, 1511–1521.
| A direct method for fatty acid methyl ester synthesis: application to wet meat tissues, oils, and feedstuffs.Crossref | GoogleScholarGoogle Scholar |
Pearce KL, Norman HC, Wilmot M, Rintoul A, Pethick DW, Masters DG (2008) The effect of grazing saltbush with a barley supplement on the carcass and eating quality of sheepmeat. Meat Science 79, 344–354.
| The effect of grazing saltbush with a barley supplement on the carcass and eating quality of sheepmeat.Crossref | GoogleScholarGoogle Scholar |
Provenza FD, Pfister JA (1991) Influence of plant toxins on food ingestion by herbivores. In ‘Recent advances on the nutrition of herbivores’. (Eds YW Ho, HK Wong, N Abdullah, ZA Tajuddin) pp. 199–206. (Malaysian Society of Animal Production: Kuala Lumpur, Malaysia)
Reed KFM (2016) Fertility of herbivores consuming phytoestrogen-containing Medicago and Trifolium species. Agriculture 6, 35
| Fertility of herbivores consuming phytoestrogen-containing Medicago and Trifolium species.Crossref | GoogleScholarGoogle Scholar |
Reid RL (1981) Livestock disease. In ‘A manual of Australian agriculture’. 4th edn. (Ed. RL Reid) pp. 489–525. (The Australian Institute of Agricultural Science, William Heinemann: Australia)
Revell C, Revell D (2007) Meeting ‘duty of care’ obligations when developing new pasture species. Field Crops Research 104, 95–102.
| Meeting ‘duty of care’ obligations when developing new pasture species.Crossref | GoogleScholarGoogle Scholar |
Schreurs NM, Lane GA, Tavendale MH, Barry TN, McNabb WC (2008) Pastoral flavour in meat products from ruminants fed fresh forages and its amelioration by forage condensed tannins. Animal Feed Science and Technology 146, 193–221.
| Pastoral flavour in meat products from ruminants fed fresh forages and its amelioration by forage condensed tannins.Crossref | GoogleScholarGoogle Scholar |
Suiter J (1994) ‘Body condition scoring of sheep and goats.’ (Farmnote, Department of Agriculture: Perth, WA, Australia)
Sweeney RA, Rexroad PR (1987) Comparison of LECO FP-228 ‘Nitrogen Determinator’ with AOAC copper catalyst Kjeldahl method for crude protein. Journal of Association of Official Analytical Chemists 70, 1028–1030.
| Comparison of LECO FP-228 ‘Nitrogen Determinator’ with AOAC copper catalyst Kjeldahl method for crude protein.Crossref | GoogleScholarGoogle Scholar |
Underwood EJ, Suttle NF (1999) ‘The mineral nutrition of livestock.’ (3rd edn). (CAB International Publishing: Wallingford, UK)
Weston RH (1996) Some aspects of constraint to forage consumption by ruminants. Australian Journal of Agricultural Research 47, 175–197.
| Some aspects of constraint to forage consumption by ruminants.Crossref | GoogleScholarGoogle Scholar |
White CL, Masters DG, Peter DW, Purser DB, Roe SP, Barnes MJ (1992) A multi element supplement for grazing sheep. I. Intake, mineral status and production responses. Australian Journal of Agricultural Research 43, 795–808.
| A multi element supplement for grazing sheep. I. Intake, mineral status and production responses.Crossref | GoogleScholarGoogle Scholar |
Yusharyahya SN, Bramono K, Hestiantoro A, Edwar SQ, Kusuma I (2020) Fenugreek (Trigonella foenum-graceum) increases postmenopausal fibroblast-associated COL1A1 and COL3A1 production dominantly through its binding to estrogen receptor beta. Journal of Applied Pharmaceutical Science 10, 22–27.
| Fenugreek (Trigonella foenum-graceum) increases postmenopausal fibroblast-associated COL1A1 and COL3A1 production dominantly through its binding to estrogen receptor beta.Crossref | GoogleScholarGoogle Scholar |