Effects of dietary supplementation with Artemisia argyi alcohol extract on growth performance, blood biochemical properties and small intestinal immune markers of broilers challenged with lipopolysaccharide
Shuo Yang A , Jing Zhang A , Yang Jiang A , Yuanqing Xu A , Xiao Jin A , Sumei Yan A and Binlin Shi
A *
+ Author Affiliations
- Author Affiliations
A College of Animal Science, Inner Mongolia Agricultural University, 306 Zhaowuda Road, Saihan District, Hohhot, Inner Mongolia 010018, People’s Republic of China.
Animal Production Science 62(3) 234-247 https://doi.org/10.1071/AN21157
Submitted: 25 March 2021 Accepted: 29 September 2021 Published: 4 November 2021
© 2022 The Author(s) (or their employer(s)). Published by CSIRO Publishing
Abstract
Context: Broilers are prone to immunological stress when subjected to unsuitable environmental conditions (such as virus attack, nutrient deficiency and high stocking density), lowering immunity and resulting in inflammatory bowel diseases. The herb-feed additive Artemisia argyi has been applied in poultry production, and its extract may improve small intestinal immune capacity.
Aims: This study was conducted to investigate the effect of A. argyi alcohol extract (AAAE) on growth performance and blood biochemical and small intestinal immune markers of broilers challenged with a proinflammatory substance, lipopolysaccharide (LPS). The study also examined possible mechanisms of action, and aimed to clarify whether AAAE could be applied as a feed additive.
Methods: In total, 192 one-day-old Arbor Acres broilers were allocated to four groups following a 2 × 2 factorial arrangement (including six replicates with eight birds per replicate) with two dietary AAAE rates (0 or 750 mg/kg) and two immune stress treatments (LPS or saline injection). Blood and small intestine were sampled on Days 21 and 35.
Key results: Dietary AAAE alleviated the LPS-induced decrease in bodyweight, average daily gain and average daily feed intake, and mitigated the elevated serum alanine aminotransferase, triglyceride, low-density lipoprotein cholesterol, adrenocorticotropic hormone and corticosterone concentrations at Day 21 and/or Day 35 in LPS-challenged broilers. AAAE significantly (P < 0.05) attenuated LPS-induced increases in intestinal immunoglobulin (IgA, IgG and IgM) and interleukin (IL-1β and IL-6) concentrations. Moreover, the small intestinal mRNA abundances of the genes TLR4, MyD88, NF-κBp65, IL-1β and IL-6 in LPS-challenged broilers were decreased (P < 0.05) in response to dietary AAAE treatment.
Conclusions: These results further demonstrated that AAAE at 750 mg/kg enhanced small intestinal tissue immune capacity of broilers, thereby alleviating LPS-induced immune stress damage in broilers. Its mechanism of action may be related to the mediating of TLR4/NF-κB pathways.
Implications: Dietary AAAE can be used to improve the immune function of broilers, and to provide a new scientific theoretical basis for the development of new anti-stress feed additives.
Keywords: Artemisia argyi, biochemical marker, broiler, growth performance, immune stress, intestine, lipopolysaccharide (LPS), TLR4/NF-κB pathway.
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