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RESEARCH ARTICLE

The effect of molasses nitrate lick blocks on supplement intake, bodyweight, condition score, blood methaemoglobin concentration and herd scale methane emissions in Bos indicus cows grazing poor quality forage

M. J. Callaghan https://orcid.org/0000-0001-6930-3905 A G , N. W. Tomkins B C , G. Hepworth D and A. J. Parker https://orcid.org/0000-0001-6370-6623 E F
+ Author Affiliations
- Author Affiliations

A Ridley AgriProducts Pty Ltd, Toowong, Brisbane, Qld 4066, Australia.

B CSIRO, Australian Tropical Science and Innovation Precinct, JCU, Townsville, Qld 4811, Australia.

C Meat and Livestock Australia, Fortitude Valley, Brisbane, Qld 4006, Australia.

D Statistical Consulting Centre, The University of Melbourne, Melbourne, Vic. 3010, Australia.

E Department of Animal Sciences, The Ohio State University, Wooster, Ohio 44691, USA.

F Department of Veterinary Science, James Cook University, Townsville, Qld 4811, Australia.

G Corresponding author. Email: matthew.callaghan@ridley.com.au

Animal Production Science 61(5) 445-458 https://doi.org/10.1071/AN20389
Submitted: 24 July 2020  Accepted: 29 October 2020   Published: 23 November 2020

Abstract

Context: The Australian government has approved a greenhouse gas (GHG) offset method that requires cattle to consume nitrate in the form of a lick block. Field studies demonstrating the effectiveness of this methodology have not been previously reported.

Aims: This experiment was conducted to determine the effects on productivity and health when nitrate lick blocks were provided as a supplement to grazing beef cattle. We hypothesised that beef cattle given access to nitrate lick blocks would have similar productivity compared with cattle offered urea lick blocks.

Methods: Bos indicus breeding cows (n = 76) grazed a 467-ha paddock near Charters Towers, Queensland, between May and November 2014. A two-way remote automatic drafting system enabled allocation of cattle to different treatments while grazing in a common paddock. Treatments were 30% urea lick blocks (30U), or molasses nitrate lick blocks (MNB). At monthly intervals liveweight (LW), body condition score (BCS), and blood methaemoglobin concentration were recorded. Estimates of individual supplement intake were made on three separate occasions using a lithium marker technique.

Results: Mean daily supplement intake (±s.e.m.) of 30U (122 ± 13 g) was greater (P < 0.001) than MNB (67 ± 8 g). Lesser MNB intake was associated with greater variability for individual supplement intake, a greater proportion of non-consumers of supplement during July (P < 0.05) and reduced voluntary supplement intake until October (P < 0.001). Increasing MNB consumption during October and November was accompanied by elevated blood methaemoglobin concentration (P < 0.001). It was estimated that cattle offered MNB had insufficient supplementary nitrogen intake throughout the study to resolve rumen degradable nitrogen deficiency from grazed forage. Consequently, cattle provided access to MNB demonstrated conceptus free liveweight loss and lesser BCS compared with cattle treated with 30U (P < 0.001).

Conclusion: Nitrate lick blocks were ineffective as a dual-purpose non-protein nitrogen supplement and methane mitigant for beef cattle grazing poor quality forage. Further field experiments are required to determine if there may be situations where this GHG offset methodology is efficacious.

Implications: Caution is advised in implementing GHG mitigation methods that involve the use of nitrate lick blocks.

Keywords: Australian Carbon Credit Units, carbon economy, cardio-vascular challenge, cattle, Emissions Reduction Fund, greenhouse gas, greenhouse gas offset, lick blocks, methane, methaemoglobinaemia, nitrate, urea, protein supplement, supplementation.


References

Ash AJ, McIvor JG, Corfield JP, Winter WH (1995) How land condition alters plant-animal relationships in Australia’s tropical rangelands. Agriculture, Ecosystems & Environment 56, 77–92.
How land condition alters plant-animal relationships in Australia’s tropical rangelands.Crossref | GoogleScholarGoogle Scholar |

Australian Greenhouse Emissions Information System (2018) National greenhouse gas inventory: Kyoto protocol classifications. Available at http://ageis.climatechange.gov.au/ [Verified 8 June 2020].

Bailey DW, Welling GR (2007) Evaluation of low-moisture blocks and conventional dry mixes for supplementing minerals and modifying cattle grazing patterns. Rangeland Ecology and Management 60, 54–64.
Evaluation of low-moisture blocks and conventional dry mixes for supplementing minerals and modifying cattle grazing patterns.Crossref | GoogleScholarGoogle Scholar |

Benu I (2017) The safety and efficacy of nitrate N supplementation to Bos indicus cattle. PhD thesis. James Cook University, Australia.

Benu I, Callaghan MJ, Tomkins N, Hepworth G, Fitzpatrick LA, Parker AJ (2016) The effect of feeding frequency and dose rate of nitrate supplements on blood haemoglobin fractions in Bos indicus cattle fed Flinders grass (Iseilema spp.) hay. Animal Production Science 56, 1605–1611.
The effect of feeding frequency and dose rate of nitrate supplements on blood haemoglobin fractions in Bos indicus cattle fed Flinders grass (Iseilema spp.) hay.Crossref | GoogleScholarGoogle Scholar |

Benu I, Fitzpatrick LA, Callaghan MJ, Tomkins N, Parker AJ (2018) The effect of nitrate supplementation on arterial blood gases, haemoglobin fractions and heart rate in Bos indicus cattle after exercise. Animal Production Science 58, 1603–1607.
The effect of nitrate supplementation on arterial blood gases, haemoglobin fractions and heart rate in Bos indicus cattle after exercise.Crossref | GoogleScholarGoogle Scholar |

Bortolussi G, McIvor JG, Hodgkinson JJ, Coffey SG, Holmes CR (2005) The northern Australian beef industry, a snapshot. 2. Breeding herd performance and management. Australian Journal of Experimental Agriculture 45, 1075–1091.
The northern Australian beef industry, a snapshot. 2. Breeding herd performance and management.Crossref | GoogleScholarGoogle Scholar |

Bowman JG, Sowell BF (1997) Delivery method and supplement consumption by grazing ruminants: a review. Journal of Animal Science 75, 543–550.
Delivery method and supplement consumption by grazing ruminants: a review.Crossref | GoogleScholarGoogle Scholar | 9051478PubMed |

Bruning-Fann CS, Kaneene JB (1993) The effects of nitrate, nitrite, and N-nitroso compounds on animal health. Veterinary and Human Toxicology 35, 237–253.

Burns BM, Fordyce G, Holroyd RG (2010) A review of factors that impact on the capacity of beef cattle females to conceive, maintain a pregnancy and wean a calf—Implications for reproductive efficiency in northern Australia. Animal Reproduction Science 122, 1–22.
A review of factors that impact on the capacity of beef cattle females to conceive, maintain a pregnancy and wean a calf—Implications for reproductive efficiency in northern Australia.Crossref | GoogleScholarGoogle Scholar | 20447780PubMed |

Burrow HM (2014) Northern Australian beef production. In ‘Beef cattle: production and trade’. (Eds D Cottle, L Kahn) pp. 161–183. (CSIRO Publishing: Melbourne, Vic., Australia)

Callaghan MJ, Parker AJ, Edwards LJ (2013) Monensin reduces consumption of urea supplement blocks. In ‘Proceedings of the Northern Beef Research Update Conference’. (Ed. E Charmley) p. 158. (Northern Australia Beef Research Council: Gympie, Qld, Australia)

Callaghan MJ, Tomkins NW, Benu I, Parker AJ (2014) How feasible is it to replace urea with nitrates to mitigate greenhouse gas emissions from extensively managed beef cattle? Animal Production Science 54, 1300–1304.
How feasible is it to replace urea with nitrates to mitigate greenhouse gas emissions from extensively managed beef cattle?Crossref | GoogleScholarGoogle Scholar |

Charmley E, Stephens ML, Kennedy PM (2008) Predicting livestock productivity and methane emissions in northern Australia: development of a bio-economic modelling approach. Australian Journal of Experimental Agriculture 48, 109–113.
Predicting livestock productivity and methane emissions in northern Australia: development of a bio-economic modelling approach.Crossref | GoogleScholarGoogle Scholar |

Charmley ESRO, Williams SRO, Moate PJ, Hegarty RS, Herd RM, Oddy VH, Reyenga P, Staunton KM, Anderson A, Hannah MC (2016) A universal equation to predict methane production of forage-fed cattle in Australia. Animal Production Science 56, 169–180.
A universal equation to predict methane production of forage-fed cattle in Australia.Crossref | GoogleScholarGoogle Scholar |

Coates DB (2004) Faecal NIRS – technology for improving nutritional management of grazing cattle. Final Report of Project NAP3.121, Meat and Livestock Australia, Sydney, NSW, Australia.

Coates DB, Dixon RM (2007) Faecal near infrared reflectance spectroscopy (F.NIRS) measurements of non-grass proportions in the diet of cattle grazing tropical rangelands. The Rangeland Journal 29, 51–63.
Faecal near infrared reflectance spectroscopy (F.NIRS) measurements of non-grass proportions in the diet of cattle grazing tropical rangelands.Crossref | GoogleScholarGoogle Scholar |

Coates DB, Dixon RM (2008) Development of NIRS analysis of faeces to estimate non-grass proportions in the diets selected by cattle grazing tropical pastures. Journal of Near Infrared Spectroscopy 16, 471–480.
Development of NIRS analysis of faeces to estimate non-grass proportions in the diets selected by cattle grazing tropical pastures.Crossref | GoogleScholarGoogle Scholar |

Cockrum RR, Austin KJ, Ludden PA, Cammack KM (2010) Effect of subacute dietary nitrate on production traits and plasma analytes in Suffolk ewes. Animal 4, 702–708.
Effect of subacute dietary nitrate on production traits and plasma analytes in Suffolk ewes.Crossref | GoogleScholarGoogle Scholar | 22444122PubMed |

Cockwill CL, McAllister TA, Olson ME, Milligan DN, Ralston BJ, Huisma C, Hand RK (2000) Individual intake of mineral and molasses supplements by cows, heifers and calves. Canadian Journal of Animal Science 80, 681–690.
Individual intake of mineral and molasses supplements by cows, heifers and calves.Crossref | GoogleScholarGoogle Scholar |

Comlaw (2015) Carbon credits (Carbon Farming Initiative) (reducing greenhouse gas emissions by feeding nitrates to beef cattle) methodology determination 2014. Available at https://www.legislation.gov.au/Details/F2015C00580 [Verified 8 June 2020]

Dixon RM, Coates DB (2005) The use of faecal NIRS to improve nutritional management of cattle in northern Australia. Recent Advances in Animal Nutrition in Australia 15, 65–75.

Dixon RM, Coates DB (2010) Diet quality estimated with faecal near infrared reflectance spectroscopy and responses to N supplementation by cattle grazing buffel grass pastures. Animal Feed Science and Technology 158, 115–125.
Diet quality estimated with faecal near infrared reflectance spectroscopy and responses to N supplementation by cattle grazing buffel grass pastures.Crossref | GoogleScholarGoogle Scholar |

Dixon RM, Doyle PT (1996) Straw and low quality roughages as drought feeds. In ‘A user’s guide to drought feeding alternatives’. (Eds J Rowe, N Cossins) pp. 61–74. (Department of Animal Science, University of New England: Armidale, NSW, Australia)

Dixon RM, White A, Fry P, Petherick JC (2003a) Effects of supplement type and previous experience on variability in intake of supplements by heifers. Australian Journal of Agricultural Research 54, 529–540.
Effects of supplement type and previous experience on variability in intake of supplements by heifers.Crossref | GoogleScholarGoogle Scholar |

Dixon RM, Smith DR, Reid A (2003b) Lithium salts as a marker of intake of supplements by cattle. Australian Journal of Experimental Agriculture 43, 37–46.
Lithium salts as a marker of intake of supplements by cattle.Crossref | GoogleScholarGoogle Scholar |

Dixon RM, Smith DR, Coates DB (2007) Using faecal NIRS to improve nutritional management of breeders in the seasonally dry tropics. Recent Advances in Animal Nutrition in Australia 16, 123–134.

Dixon RM, Playford C, Coates DB (2011) Nutrition of beef breeder cows in the dry tropics. 1. Effects of nitrogen supplementation and weaning on breeder performance. Animal Production Science 51, 515–528.
Nutrition of beef breeder cows in the dry tropics. 1. Effects of nitrogen supplementation and weaning on breeder performance.Crossref | GoogleScholarGoogle Scholar |

Ducker MJ, Kendall PT, Hemingway RG, McClelland TH (1981) An evaluation of feed blocks as a means of providing supplementary nutrients to ewes grazing upland/hill pastures. Animal Science 33, 51–57.
An evaluation of feed blocks as a means of providing supplementary nutrients to ewes grazing upland/hill pastures.Crossref | GoogleScholarGoogle Scholar |

Eggington AR, McCosker TH, Graham CA (1990) Intake of lick block supplements by cattle grazing native monsoonal tallgrass pastures in the Northern Territory. Australian Rangeland Journal 12, 7–13.
Intake of lick block supplements by cattle grazing native monsoonal tallgrass pastures in the Northern Territory.Crossref | GoogleScholarGoogle Scholar |

Evelyn KA, Malloy HT (1938) Microdetermination of oxyhemoglobin, methemoglobin, and sulfhemoglobin in a single sample of blood. The Journal of Biological Chemistry 136, 655–662.

Farra PA, Satter LD (1971) Manipulation of the ruminal fermentation. III. Effect of nitrate on ruminal volatile fatty acid production and milk composition. Journal of Dairy Science 54, 1018–1024.
Manipulation of the ruminal fermentation. III. Effect of nitrate on ruminal volatile fatty acid production and milk composition.Crossref | GoogleScholarGoogle Scholar |

Flesch TK, Wilson JD, Harper LA, Crenna BP, Sharpe RR (2004) Deducing ground-air emissions from observed trace gas concentrations: a field trial. Journal of Applied Meteorology and Climatology 43, 487–502.
Deducing ground-air emissions from observed trace gas concentrations: a field trial.Crossref | GoogleScholarGoogle Scholar |

Flesch TK, McGinn SM, Chen D, Wilson JD, Desjardins RL (2013) Data filtering in bLS calculation of emissions rates: Improvements to increase data retention. In ‘Advances in Animal Biosciences: Proceedings of the 5th Greenhouse Gases and Animal Agriculture conference (GGAA 2013)’, Dublin, Ireland. Vol. 4. Part 2. p 472. (Cambridge University Press: Cambridge, UK)

Goopy JP, Hegarty RS (2019) Nitrate is safe to feed ad libitum in molasses roller drums as a source of non-protein nitrogen. Animal Production Science 59, 1288–1292.
Nitrate is safe to feed ad libitum in molasses roller drums as a source of non-protein nitrogen.Crossref | GoogleScholarGoogle Scholar |

Graham CA, Pern C, Linehan KL (1977) Individual daily consumption of a medicated bloat block. Australian Journal of Experimental Agriculture 17, 562–565.
Individual daily consumption of a medicated bloat block.Crossref | GoogleScholarGoogle Scholar |

Hennessy DW, Williamson PJ, Darnell RE (2000) Feed intake and liveweight responses to nitrogen and/or protein supplements by steers of Bos taurus, Bos indicus and Bos taurus × Bos indicus breed types offered a low quality grass hay. The Journal of Agricultural Science 135, 35–45.
Feed intake and liveweight responses to nitrogen and/or protein supplements by steers of Bos taurus, Bos indicus and Bos taurus × Bos indicus breed types offered a low quality grass hay.Crossref | GoogleScholarGoogle Scholar |

Hill JO, Coates DB, Whitbread AM, Clem RL, Robertson MJ, Pengelly BC (2009) Seasonal changes in pasture quality and diet selection and their relationship with liveweight gain of steers grazing tropical grass and grass–legume pastures in northern Australia. Animal Production Science 49, 983–993.
Seasonal changes in pasture quality and diet selection and their relationship with liveweight gain of steers grazing tropical grass and grass–legume pastures in northern Australia.Crossref | GoogleScholarGoogle Scholar |

Hill J, McSweeney C, Wright ADG, Bishop-Hurley G, Kalantar-zadeh K (2016) Measuring methane production from ruminants. Trends in Biotechnology 34, 26–35.
Measuring methane production from ruminants.Crossref | GoogleScholarGoogle Scholar | 26603286PubMed |

Holtenius P (1957) Nitrite poisoning in sheep, with special reference to the detoxification of nitrite in the rumen: an experimental study. Acta Agriculturae Scandinavica 7, 113–163.
Nitrite poisoning in sheep, with special reference to the detoxification of nitrite in the rumen: an experimental study.Crossref | GoogleScholarGoogle Scholar |

Horadagoda A, Fulkerson WJ, Nandra KS, Barchia IM (2009) Grazing preferences by dairy cows for 14 forage species. Animal Production Science 49, 586–594.
Grazing preferences by dairy cows for 14 forage species.Crossref | GoogleScholarGoogle Scholar |

Kahn L (1994) The use of lithium chloride for estimating supplement intake in grazing sheep: estimates of heritability and repeatability. Australian Journal of Agricultural Research 45, 1731–1739.
The use of lithium chloride for estimating supplement intake in grazing sheep: estimates of heritability and repeatability.Crossref | GoogleScholarGoogle Scholar |

Kendall PT, Ducker MJ, Hemingway RG (1980) Individual intake variation by cattle given self-help feed blocks or cubed concentrate fed in troughs. In ‘Titles and abstracts of papers for presentation at the 74th meeting of the British Society of Animal Production’. p. 485. (The British Society of Animal Production: Reading, UK)

Lee C, Beauchemin KA (2014) A review of feeding supplementary nitrate to ruminant animals: nitrate toxicity, methane emissions and production performance. Canadian Journal of Animal Science 94, 557–570.
A review of feeding supplementary nitrate to ruminant animals: nitrate toxicity, methane emissions and production performance.Crossref | GoogleScholarGoogle Scholar |

Lee C, Araujo RC, Koenig KM, Beauchemin KA (2015) Effects of encapsulated nitrate on eating behaviour, rumen fermentation, and blood profile of beef heifers fed restrictively or ad libitum. Journal of Animal Science 93, 2405–2418.
Effects of encapsulated nitrate on eating behaviour, rumen fermentation, and blood profile of beef heifers fed restrictively or ad libitum.Crossref | GoogleScholarGoogle Scholar | 26020336PubMed |

Leng RA (2008) The potential of feeding nitrate to reduce enteric methane production in ruminants. Report to Department of Climate Change, Commonwealth Government, Canberra, ACT, Australia. Available at http://www.penambulbooks.com/Downloads/Leng-Final%20Modified%20%2017-9-2008.pdf [Verified 8 June 2020]

Malestein A, Geurink JH, Schuyt G, Schotman AJH, Kemp ATh, van ‘t Klooste A (1980) Nitrate poisoning in cattle: 4. The effect of nitrine dosing during parturition on the oxygen capacity of maternal blood and the oxygen supply to the unborn calf. The Veterinary Quarterly 2, 149–159.
Nitrate poisoning in cattle: 4. The effect of nitrine dosing during parturition on the oxygen capacity of maternal blood and the oxygen supply to the unborn calf.Crossref | GoogleScholarGoogle Scholar | 22039936PubMed |

McGowan MR, McCosker K, Fordyce G, Smith D, O’Rourke PK, Perkins N, Barnes T, Marquet L, Morton J, Newsome T, Menzies D (2014) North Australian beef fertility project: Cash Cow. Final Report, Project B. NBP. 0382, Meat and Livestock Australia, Sydney, NSW, Australia.

McLennan SR (1999) New techniques for estimating supplement intake by grazing herbivores. In ‘Nutritional ecology of herbivores. Satellite symposium: emerging techniques for studying the nutrition of free ranging herbivores’. (CD-ROM) (Eds H Dove, SW Coleman) (American Society of Animal Science: San Antonio, TX, USA)

McLennan SR, Dunster PJ, O’Rourke PK, Murphy GM (1981) Comparison of dry season urea supplements containing salt, sulfur or molasses for steers grazing native pasture in the dry tropics of northern Queensland. Australian Journal of Experimental Agriculture 21, 457–463.
Comparison of dry season urea supplements containing salt, sulfur or molasses for steers grazing native pasture in the dry tropics of northern Queensland.Crossref | GoogleScholarGoogle Scholar |

Meat and Livestock Australia (2019) Fast facts 2019 Australia’s beef industry. Available at https://www.mla.com.au/prices-markets/Trends-analysis/fast-facts/ [Verified 8 June 2020]

Mulholland JG, Coombe JB (1979) Supplementation of sheep grazing wheat stubble with urea, molasses and minerals: quality of diet, intake of supplements and animal response. Australian Journal of Experimental Agriculture 19, 23–31.
Supplementation of sheep grazing wheat stubble with urea, molasses and minerals: quality of diet, intake of supplements and animal response.Crossref | GoogleScholarGoogle Scholar |

Newbold JR, Van Zijderveld SM, Hulshof RBA, Fokkink WB, Leng RA, Terencio P, Powers WJ, Van Adrichem PSJ, Paton ND, Perdok HB (2014) The effect of incremental levels of dietary nitrate on methane emissions in Holstein steers and performance in Nelore bulls. Journal of Animal Science 92, 5032–5040.
The effect of incremental levels of dietary nitrate on methane emissions in Holstein steers and performance in Nelore bulls.Crossref | GoogleScholarGoogle Scholar | 25349351PubMed |

O’Rourke PK, Entwistle KW, Arman C, Esdale CR, Burns BM (1991) Fetal development and gestational changes in Bos taurus and Bos indicus genotypes in the tropics. Theriogenology 36, 839–853.
Fetal development and gestational changes in Bos taurus and Bos indicus genotypes in the tropics.Crossref | GoogleScholarGoogle Scholar | 16727053PubMed |

Parkinson TJ, Vermunt JJ, Malmo J (2010) ‘Diseases of cattle in Australasia: a comprehensive textbook.’ (Vetlearn: Wellington, New Zealand)

Pfister JA, Provenza FD, Manners GD, Gardner DR, Ralphs MH (1997) Tall larkspur ingestion: can cattle regulate intake below toxic levels? Journal of Chemical Ecology 23, 759–777.
Tall larkspur ingestion: can cattle regulate intake below toxic levels?Crossref | GoogleScholarGoogle Scholar |

Provenza FD (1995) Post-ingestive feedback as an elementary determinant of food preference and intake in ruminants. Journal of Range Management 48, 2–17.
Post-ingestive feedback as an elementary determinant of food preference and intake in ruminants.Crossref | GoogleScholarGoogle Scholar |

Provenza FD, Bowns JE, Urness PJ, Malechek JC, Butcher JE (1983) Biological manipulation of blackbrush by goat browsing. Journal of Range Management 36, 513–518.
Biological manipulation of blackbrush by goat browsing.Crossref | GoogleScholarGoogle Scholar |

Provenza FD, Villalba JJ, Haskell J, MacAdam JW, Griggs TC, Wiedmeier RD (2007) The value to herbivores of plant physical and chemical diversity in time and space. Crop Science 47, 382–398.
The value to herbivores of plant physical and chemical diversity in time and space.Crossref | GoogleScholarGoogle Scholar |

Roth CH, Prosser IP, Post DA, Gross JE, Webb MJ (2003) Reducing sediment export from the Burdekin catchment. Final Report of Project NAP3.224. Meat and Livestock Australia. Sydney, NSW, Australia.

Stobbs TH (1975) Factors limiting the nutritional value of grazed tropical pastures for beef and milk production. Tropical Grasslands 9, 141–150.

Tomkins NW, Charmley E (2015) Herd-scale measurements of methane emissions from cattle grazing extensive sub-tropical grasslands using the open-path laser technique. Animal 9, 2029–2038.
Herd-scale measurements of methane emissions from cattle grazing extensive sub-tropical grasslands using the open-path laser technique.Crossref | GoogleScholarGoogle Scholar | 26290115PubMed |

Tomkins NW, McGinn SM, Turner DA, Charmley E (2011) Comparison of open-circuit respiration chambers with a micrometeorological method for determining methane emissions from beef cattle grazing a tropical pasture. Animal Feed Science and Technology 166, 240–247.
Comparison of open-circuit respiration chambers with a micrometeorological method for determining methane emissions from beef cattle grazing a tropical pasture.Crossref | GoogleScholarGoogle Scholar |

Tomkins N, Parker AJ, Hepworth G, Callaghan MJ (2018) Nitrate supplementation has marginal effects on enteric methane production from Bos indicus steers fed Flinders grass (Iseilema spp.) hay, but elevates blood methaemoglobin concentrations. Animal Production Science 58, 262–270.
Nitrate supplementation has marginal effects on enteric methane production from Bos indicus steers fed Flinders grass (Iseilema spp.) hay, but elevates blood methaemoglobin concentrations.Crossref | GoogleScholarGoogle Scholar |

Tothill JC, Hargreaves JN, Jones RM, McDonald CK (1992) BOTANAL – a comprehensive sampling and computing procedure for estimating pasture yield and composition 1. Field Sampling. Technical Memo No. 78. CSIRO, Melbourne, Vic., Australia.

Ungerfeld EM, Kohn RA (2006) The role of thermodynamics in the control of rumen fermentation. In ‘Ruminant physiology: digestion, metabolism and impact of nutrition on gene expression, immunology and stress’. (Eds K Sejrsen, T Hvelplund, MO Nielsen) pp. 55–85. (Wageningen Academic Publishers: Wageningen, Netherlands)

Velazco JI, Cottle DJ, Hegarty RS (2014) Methane emissions and feeding behaviour of feedlot cattle supplemented with nitrate or urea. Animal Production Science 54, 1737–1740.
Methane emissions and feeding behaviour of feedlot cattle supplemented with nitrate or urea.Crossref | GoogleScholarGoogle Scholar |

Winks L (1984) ‘Cattle growth in the dry tropics of Australia. Review No 45.’ (Australia Meat Research Committee: Sydney, NSW, Australia)

Winks L, Laing AR, O’Rourke PK, Wright GS (1979) Factors affecting response to urea-molasses supplements by yearling cattle in tropical Queensland. Australian Journal of Experimental Agriculture and Animal Husbandry 19, 522–529.
Factors affecting response to urea-molasses supplements by yearling cattle in tropical Queensland.Crossref | GoogleScholarGoogle Scholar |

Yang C, Rooke JA, Cabeza I, Wallace RJ (2016) Nitrate and inhibition of ruminal methanogenesis: microbial ecology, obstacles, and opportunities for lowering methane emissions from ruminant livestock. Frontiers in Microbiology 7, 132
Nitrate and inhibition of ruminal methanogenesis: microbial ecology, obstacles, and opportunities for lowering methane emissions from ruminant livestock.Crossref | GoogleScholarGoogle Scholar | 26904008PubMed |

Zhu XS, Deyoe CW (1991) Effects of various ingredients on the manufacture of poured feed block containing a distillery by-product. Animal Feed Science and Technology 34, 229–239.
Effects of various ingredients on the manufacture of poured feed block containing a distillery by-product.Crossref | GoogleScholarGoogle Scholar |