Effects of phytase supplementation on growth performance, plasma biochemistry, bone mineralisation and phosphorus utilisation in pre-lay pullets fed various levels of phosphorus
Mingyan Jing A , Shusheng Zhao A , Anna Rogiewicz B , Bogdan A. Slominski B and James D. House
A B C
+ Author Affiliations
- Author Affiliations
A Department of Food and Human Nutritional Sciences, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada.
B Department of Animal Science, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada.
C Corresponding author. Email: James.House@umanitoba.ca
Animal Production Science 61(6) 568-576 https://doi.org/10.1071/AN20265
Submitted: 9 June 2020 Accepted: 24 November 2020 Published: 23 February 2021
Journal Compilation © CSIRO 2021 Open Access CC BY-NC-ND
Abstract
Context: Reducing the environmental impact of animal production is becoming a really hot topic, especially with raised concerns over excessive flows of nitrogen and phosphorus (P) to the environment.
Aims: The present study was conducted to determine the effects of phytase supplementation on growth, plasma biochemistry, bone mineralisation and P utilisation of pre-lay pullets fed varying levels of non-phytate P.
Methods: A total of 240 Lohmann pullet chicks were randomly allocated to one of six dietary treatments with eight replicate cages (5 birds per cage) per treatment. Six treatments included three phytase-free diets and three diets supplemented with 1000 U/kg phytase; the non-phytate P levels were 2.75–2.50–2.25, 3.75–3.50–3.25 and 4.75–4.50–4.25 g/kg in the former, and 1.75–1.50–1.25, 2.75–2.50–2.25 and 3.75–3.50–3.25 g/kg in the latter, for the age of 0–4, 4–8 and 8–16 weeks respectively.
Key results: No significant differences were found for growth performance, plasma biochemistry (calcium, P, alkaline phosphatase and albumin) and bone mineralisation among dietary treatments, but P retention (%) was different (P < 0.001). Analysis of planned contrasts showed that phytase supplementation increased phytate P retention (P < 0.001), and improving the utilisation of phytate P tended most efficiently under low P conditions. Total P retention rate was reduced slightly by phytase supplementation (P < 0.05).
Conclusions: The results indicated that dietary non-phytate P level could possibly be reduced to 1.75, 1.50 and 1.25 g/kg for 0–4, 4–8 and 8–16 weeks of age respectively after phytase supplementation, without compromising pullet growth and performance during the pre-laying period.
Implications: The results of this study will contribute to decreasing P excretion by poultry and reducing the potential environmental impact with land application of manure.
Keywords: characteristics of bones, growth, phosphorus retention, phytase.
References
Ankra-Badu GA, Aggrey SE, Pesti GM, Bakalli RI, Edwards HM (2004) Modeling of parameters affecting phytate phosphorus bioavailability in growing birds. Poultry Science 83, 1083–1088.| Modeling of parameters affecting phytate phosphorus bioavailability in growing birds.Crossref | GoogleScholarGoogle Scholar | 15285496PubMed |
AOAC International (2005) ‘Association of official analytical chemists. Official methods of analysis.’ 18th edn. (AOAC International: Washington, DC)
Bhatnagar V, Richard EL, Wu W, Nievergelt CM, Lipkowitz MS, Jeff J, Maihofer AX, Nigam SK (2016) Analysis of ABCG2 and other urate transporters in uric acid homeostasis in chronic kidney disease: potential role of remote sensing and signaling. Clinical Kidney Journal 9, 444–453.
| Analysis of ABCG2 and other urate transporters in uric acid homeostasis in chronic kidney disease: potential role of remote sensing and signaling.Crossref | GoogleScholarGoogle Scholar | 27274832PubMed |
Boling SD, Douglas MW, Johnson ML, Wang X, Parsons CM, Koelkebeck KW, Zimmerman RA (2000) The effects of dietary available phosphorus levels and phytase on performance of young and older laying hens. Poultry Science 79, 224–230.
| The effects of dietary available phosphorus levels and phytase on performance of young and older laying hens.Crossref | GoogleScholarGoogle Scholar | 10735751PubMed |
CCAC (2009) ‘Guidelines on: the care and use of farm animals in research, teaching and testing.’ Vol. II. (Canadian Council on Animal Care: Ottawa)
Dersjant-Li Y, Awati A, Schulze H, Partridge G (2015) Phytase in non-ruminant animal nutrition: a critical review on phytase activities in the gastrointestinal tract and influencing factors. Journal of the Science of Food and Agriculture 95, 878–896.
| Phytase in non-ruminant animal nutrition: a critical review on phytase activities in the gastrointestinal tract and influencing factors.Crossref | GoogleScholarGoogle Scholar | 25382707PubMed |
Douglas CR, Harms RH (1986) Phosphorus requirement of commercial Leghorn pullets from 8 to 20 weeks. Poultry Science 65, 2366–2368.
| Phosphorus requirement of commercial Leghorn pullets from 8 to 20 weeks.Crossref | GoogleScholarGoogle Scholar | 3575224PubMed |
Gordon RW, Roland DA (1997) Performance of commercial laying hens fed various phosphorus levels with and without supplemental phytase. Poultry Science 76, 1172–1177.
| Performance of commercial laying hens fed various phosphorus levels with and without supplemental phytase.Crossref | GoogleScholarGoogle Scholar | 9251148PubMed |
Haug W, Lantzsch H-J (1983) Sensitive method for the rapid determination of phytate in cereals and cereal products. Journal of the Science of Food and Agriculture 34, 1423–1426.
| Sensitive method for the rapid determination of phytate in cereals and cereal products.Crossref | GoogleScholarGoogle Scholar |
Jing M, Zhao S, Rogiewicz A, Slominski BA, House JD (2018a) Assessment of the minimal available phosphorus needs of laying hens: implications for phosphorus management strategies. Poultry Science 97, 2400–2410.
| Assessment of the minimal available phosphorus needs of laying hens: implications for phosphorus management strategies.Crossref | GoogleScholarGoogle Scholar | 29617962PubMed |
Jing M, Zhao S, Rogiewicz A, Slominski BA, House JD (2018b) Assessment of the minimal available phosphorus needs of pullets during the pre-laying period. Poultry Science 97, 557–567.
| Assessment of the minimal available phosphorus needs of pullets during the pre-laying period.Crossref | GoogleScholarGoogle Scholar | 29077938PubMed |
Keshavarz K (1998) Further investigations on the effect of dietary manipulation of protein, phosphorus, and calcium for reducing their daily requirement for laying hens. Poultry Science 77, 1333–1346.
| Further investigations on the effect of dietary manipulation of protein, phosphorus, and calcium for reducing their daily requirement for laying hens.Crossref | GoogleScholarGoogle Scholar | 9733121PubMed |
Keshavarz K (2000a) Nonphytate phosphorus requirement of laying hens with and without phytase on a phase feeding program. Poultry Science 79, 748–763.
| Nonphytate phosphorus requirement of laying hens with and without phytase on a phase feeding program.Crossref | GoogleScholarGoogle Scholar | 10824965PubMed |
Keshavarz K (2000b) Reevaluation of nonphytate phosphorus requirement of growing pullets with and without phytase. Poultry Science 79, 1143–1153.
| Reevaluation of nonphytate phosphorus requirement of growing pullets with and without phytase.Crossref | GoogleScholarGoogle Scholar | 10947183PubMed |
Leske KL, Coon CN (1999) A bioassay to determine the effect of phytase and phytate-phosphorus hydrolysis and total phosphorus retention of feed ingredients as determined with broilers and laying hens. Poultry Science 78, 1151–1157.
| A bioassay to determine the effect of phytase and phytate-phosphorus hydrolysis and total phosphorus retention of feed ingredients as determined with broilers and laying hens.Crossref | GoogleScholarGoogle Scholar | 10472841PubMed |
Li W, Angel R, Kim SW, Brady K, Yu S, Plumstead PW (2016) Impacts of dietary calcium, phytate, and nonphytate phosphorus concentrations in the presence or absence of phytase on inositol hexakisphosphate (IP6) degradation in different segments of broilers digestive tract. Poultry Science 95, 581–589.
| Impacts of dietary calcium, phytate, and nonphytate phosphorus concentrations in the presence or absence of phytase on inositol hexakisphosphate (IP6) degradation in different segments of broilers digestive tract.Crossref | GoogleScholarGoogle Scholar | 26740131PubMed |
Lim HS, Namkung H, Paik IK (2003) Effects of phytase supplementation on the performance, egg quality, and phosphorous excretion of laying hens fed different levels of dietary calcium and nonphytate phosphorous. Poultry Science 82, 92–99.
| Effects of phytase supplementation on the performance, egg quality, and phosphorous excretion of laying hens fed different levels of dietary calcium and nonphytate phosphorous.Crossref | GoogleScholarGoogle Scholar | 12580249PubMed |
Maddaiah VT, Kurnick AA, Reid BL (1964) Phytic acid studies. Proceedings of the Society for Experimental Biology and Medicine 115, 391–393.
| Phytic acid studies.Crossref | GoogleScholarGoogle Scholar | 14121619PubMed |
Marounek M, Skřivan M, Dlouhá G, Břeňová N (2008) Availability of phytate phosphorus and endogenous phytase activity in the digestive tract of laying hens 20 and 47 weeks old. Animal Feed Science and Technology 146, 353–359.
| Availability of phytate phosphorus and endogenous phytase activity in the digestive tract of laying hens 20 and 47 weeks old.Crossref | GoogleScholarGoogle Scholar |
Marounek M, Skřivan M, Rosero O, Rop O (2010) Intestinal and total tract phytate digestibility and phytase activity in the digestive tract of hens fed a wheat-maize-soybean diet. Journal of Animal and Feed Sciences 19, 430–439.
| Intestinal and total tract phytate digestibility and phytase activity in the digestive tract of hens fed a wheat-maize-soybean diet.Crossref | GoogleScholarGoogle Scholar |
Neijat M, House JD, Guenter W, Kebreab E (2011) Calcium and phosphorus dynamics in commercial laying hens housed in conventional or enriched cage systems. Poultry Science 90, 2383–2396.
| Calcium and phosphorus dynamics in commercial laying hens housed in conventional or enriched cage systems.Crossref | GoogleScholarGoogle Scholar | 21934024PubMed |
NRC (1994) ‘Nutrient requirements of domestic animals. Nutrient requirements of poultry.’ 9th rev. edn. (National Research Council, National Academy Press: Washington, DC)
Punna S, Roland DA (1999) Influence of supplemental microbial phytase on first cycle laying hens fed phosphorus-deficient diets from day one of age. Poultry Science 78, 1407–1411.
| Influence of supplemental microbial phytase on first cycle laying hens fed phosphorus-deficient diets from day one of age.Crossref | GoogleScholarGoogle Scholar | 10536789PubMed |
Ravindran V, Cabahug S, Ravindran G, Selle PH, Bryden WL (2000) Response of broiler chickens to microbial phytase supplementation as influenced by dietary phytic acid and non-phytate phosphorus levels. II. Effects on apparent metabolisable energy, nutrient digestibility and nutrient retention. British Poultry Science 41, 193–200.
| Response of broiler chickens to microbial phytase supplementation as influenced by dietary phytic acid and non-phytate phosphorus levels. II. Effects on apparent metabolisable energy, nutrient digestibility and nutrient retention.Crossref | GoogleScholarGoogle Scholar | 10890216PubMed |
Sakoh T, Nakayama M, Tsuchihashi T, Yoshitomi R, Tanaka S, Katafuchi E, Fukui A, Shikuwa Y, Anzai N, Kitazono T, Tsuruya K (2016) Associations of fibroblast growth factor 23 with urate metabolism in patients with chronic kidney disease. Metabolism: Clinical and Experimental 65, 1498–1507.
| Associations of fibroblast growth factor 23 with urate metabolism in patients with chronic kidney disease.Crossref | GoogleScholarGoogle Scholar |
Selle PH, Ravindran V (2007) Microbial phytase in poultry nutrition. Animal Feed Science and Technology 135, 1–41.
| Microbial phytase in poultry nutrition.Crossref | GoogleScholarGoogle Scholar |
Slominski BA (2011) Recent advances in research on enzymes for poultry diets. Poultry Science 90, 2013–2023.
| Recent advances in research on enzymes for poultry diets.Crossref | GoogleScholarGoogle Scholar | 21844268PubMed |
Sugimoto R, Watanabe H, Ikegami K, Enoki Y, Imafuku T, Sakaguchi Y, Murata M, Nishida K, Miyamura S, Ishima Y, Tanaka M, Matsushita K, Komaba H, Fukagawa M, Otagiri M, Maruyama T (2017) Downregulation of ABCG2, a urate exporter, by parathyroid hormone enhances urate accumulation in secondary hyperparathyroidism. Kidney International 91, 658–670.
| Downregulation of ABCG2, a urate exporter, by parathyroid hormone enhances urate accumulation in secondary hyperparathyroidism.Crossref | GoogleScholarGoogle Scholar | 27988213PubMed |
Tierzucht L Management guide, cage housing. Available at http://www.winmixsoft.com/files/info/Lohman-lsl-classic-management-guide-eng.pdf. [Verified 23 March 2015]
Viveros A, Brenes A, Arija I, Centeno C (2002) Effects of microbial phytase supplementation on mineral utilization and serum enzyme activities in broiler chicks fed different levels of phosphorus. Poultry Science 81, 1172–1183.
| Effects of microbial phytase supplementation on mineral utilization and serum enzyme activities in broiler chicks fed different levels of phosphorus.Crossref | GoogleScholarGoogle Scholar | 12211310PubMed |
