Sika deer (Cervus nippon) velvet antler ameliorates 2,4-dinitrochlorobenzene-induced atopic dermatitis-like clinical signs in a rodent model
Young-Jin Choi A , Meiqi Fan A , Yonghai Yu B , Xiaoli Wang B , Yujiao Tang A B C and Eun-Kyung Kim
A C
+ Author Affiliations
- Author Affiliations
A Division of Food Bioscience, College of Biomedical and Health Sciences, Konkuk University, 268 Chungwondaero, Chungju 27478, Republic of Korea.
B School of Bio-science and Food Engineering, Changchun University of Science and Technology, 7089 Weixing Road, Changchun 130-600, China.
C Corresponding author. Email: yuanxi00@126.com; eunkyungkim@kku.ac.kr
Animal Production Science 60(10) 1357-1363 https://doi.org/10.1071/AN19500
Submitted: 12 September 2019 Accepted: 18 January 2020 Published: 13 May 2020
Abstract
Context: Deer velvet is a rarely used component in traditional Chinese medicine and has beneficial effects against several diseases. As a substance that covers the bone and cartilage of immature antlers, deer velvet is a natural cytokine ‘storeroom’ that is rich in protein and proteoglycans. Recently, proteoglycans have been shown to have beneficial effects against inflammation.
Aims: To determine whether antler extract possesses therapeutic effects in a mouse model of atopic dermatitis (AD) and to explore the underlying mechanisms of action.
Methods: BALB/c mice were randomly divided into the following groups: control, AD, and AD + antler groups. We established an in vivo AD model by repeatedly exposing the ears of mice to Dermatophagoides farinae extract (house dust-mite extract) and 2,4-dinitrochlorobenzene once per week for 4 weeks. On the day after induction, ear thickness was measured. Antler extract (100 mg/kg) was administered orally once a day for 26 days. After 4 weeks of treatment with antler extract, the epidermal and dermal ear thickness, mast-cell infiltration, spleen weight, and lymph-node weight were measured. In addition, the mRNA levels of several pathogenic cytokines in the ears were measured. The concentrations of IL-4, IL-5, IL-10, IL-31 and IL-17 mRNA in the skin lesions of each group were measured by quantitative polymerase chain reaction.
Key results: Epidermal and dermal ear thickness, mast-cell infiltration, lymph-node weight, and gene expression levels of pathogenic cytokines in ear tissue were diminished following oral administration of antler extract, unlike in the control group.
Conclusions: The results of the present study strongly suggest that antler extract exhibits therapeutic activity against atopic dermatitis via regulation of inflammatory response.
Implications: Further exploration of the mechanisms of action of antler extract will be important for clinical application.
Additional keywords: allergic disease, inflammation. skin disease.
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