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RESEARCH ARTICLE

Quantifying the ruminal degradation of nutrients in three cultivars of perennial ryegrass (Lolium perenne L.) sampled during early spring and summer

M. L. Douglas https://orcid.org/0000-0002-4709-4572 A B C , M. J. Auldist A , J. L. Jacobs A , M. C. Hannah A , S. C. Garcia B and W. J. Wales A
+ Author Affiliations
- Author Affiliations

A Agriculture Victoria Research, Ellinbank, Vic. 3821, Australia.

B Dairy Science Group, School of Life and Environmental Sciences, The University of Sydney, Camden, NSW 2570, Australia.

C Corresponding author. Email: meaghan.douglas@agriculture.vic.gov.au

Animal Production Science 60(3) 370-378 https://doi.org/10.1071/AN19002
Submitted: 11 January 2019  Accepted: 16 June 2019   Published: 27 November 2019

Abstract

Context: The Australian dairy industry is characterised by pasture-based feeding systems, where grazed pasture is commonly supplemented with cereal grain to increase milk production of dairy cows. Perennial ryegrass (PRG; Lolium perenne L.) is the most commonly used pasture species, with a wide range of cultivars available. However, the nutritive characteristics and the degradation of nutrients in individual PRG cultivars grown in different environments in Australia are unknown, and significant variation between cultivars may impact on supplementary nutrient requirements.

Aims: The objective of this experiment was to quantify the extent of ruminal degradation of nutrients in three contrasting PRG cultivars (Bealey NEA2, Trojan NEA2 and Victorian SE) harvested during early spring and summer from Gippsland, northern Victoria and south-west Victoria in Victoria, Australia.

Methods: Degradation parameters were determined by in situ incubation in the rumens of non-lactating, rumen-fistulated Holstein–Friesian cows for 72 h using a nylon bag technique.

Key results: During both seasons, Bealey NEA2 had the greatest effective degradability of crude protein and neutral detergent fibre compared with Trojan NEA2 and Victorian SE. Cultivars harvested during early spring had greater effective degradability of crude protein and neutral detergent fibre, and a greater amount of rumen degradable protein compared with cultivars harvested during summer. Cultivars harvested from Gippsland had greater crude protein and neutral detergent fibre effective degradability, as well as a greater amount of rumen degradable protein, whereas cultivars harvested from northern Victoria had a greater amount of undegraded dietary protein.

Conclusions: These results demonstrate that the ruminal degradation of PRG cultivars declines with increasing maturity, and that there are differences between cultivars and regions; however, differences between seasons are of a greater magnitude and are more important to consider when formulating optimal supplementary grain rations.

Implications: This research has positive implications for farmers who will be able to understand the degradation of nutrients in PRG, and the amounts of rumen degradable protein and undegraded dietary protein available from pasture during each season. Farmers will be able to use this information to formulate supplementary grain rations that complement the nutrients from pasture and optimise milk production.

Additional keywords: dairy cow, digestibility, ruminant nutrition.


References

Bargo F, Muller LD, Varga GA, Delahoy JE, Cassidy TW (2002) Ruminal digestion and fermentation of high-producing dairy cows with three different feeding systems combining pasture and total mixed rations. Journal of Dairy Science 85, 2964–2973.
Ruminal digestion and fermentation of high-producing dairy cows with three different feeding systems combining pasture and total mixed rations.Crossref | GoogleScholarGoogle Scholar | 12487462PubMed |

Charlton JFL, Stewart AV (1999) Pasture species and cultivars used in New Zealand – a list. In ‘Proceedings of the New Zealand Grassland Association’, Hawkes Bay. pp. 147–166. (New Zealand Grassland Association: Wellington)

CSIRO (2007) ‘Nutrient requirements of domesticated ruminants.’ (CSIRO Publishing: Melbourne)

Douglas ML, Auldist MJ, Jacobs JL, Kelly KB, Lawson AR, Leddin CM, García SC, Wales WJ (2018) Regional variation in the nutritive characteristics of perennial ryegrass in the three dairying regions of Victoria. In ‘Australasian Dairy Science Symposium 2018: dairy science for profitable and sustainable farming’, 21–23 November 2018, Palmerston North. pp. 175–178. (Australasian Dairy Science Symposium: Palmerston North)

Doyle PT, Stockdale CR, Lawson A, Cohen DC (2000) ‘Pastures for dairy production in Victoria.’ (Kyabram Dairy Centre: Kyabram, Vic.)

Earle DF (1976) A guide to scoring dairy cow condition. Journal of Agriculture Victoria 74, 228–231.

Fox DG, Barry MC, Pitt RE, Roseler DK, Stone WC (1995) Application of the Cornell Net Carbohydrate and Protein System for cattle consuming forages. Journal of Animal Science 73, 267–277.
Application of the Cornell Net Carbohydrate and Protein System for cattle consuming forages.Crossref | GoogleScholarGoogle Scholar | 7601743PubMed |

Gately T (1984) Early versus late perennial ryegrass (Lolium perenne) for milk production. Israel Journal of Agricultural Research 23, 1–11.

Herrera-Saldana R, Gomez-Alarcon R, Torabi M, Huber JT (1990) Influence of synchronizing protein and starch degradation in the rumen on nutrient utilization and microbial protein synthesis. Journal of Dairy Science 73, 142–148.
Influence of synchronizing protein and starch degradation in the rumen on nutrient utilization and microbial protein synthesis.Crossref | GoogleScholarGoogle Scholar | 2312880PubMed |

Hoffman PC, Sievert SJ, Shaver RD, Welch DA, Combs DK (1993) In situ dry matter, protein, and fiber degradation of perennial forages. Journal of Dairy Science 76, 2632–2643.
In situ dry matter, protein, and fiber degradation of perennial forages.Crossref | GoogleScholarGoogle Scholar | 8227665PubMed |

Kaufman W, Lupping W (1982) Protected proteins and protected amino acids for ruminants. In ‘Protein contribution of feedstuffs for ruminants’. (Eds EL Miller, IH Pike, AJH Vanes) pp. 36–68 (Buttersworth: London, UK)

Kellaway RC, Harrington T (2004) Protein supplements. In ‘Feeding concentrates: supplements for dairy cows’. (Ed. T Harrington) pp. 55–78 (Landlinks Press: Melbourne)

Lee JM, Matthew C, Thom ER, Chapman DF (2012) Perennial ryegrass breeding in New Zealand: a dairy industry perspective. Crop and Pasture Science 63, 107–127.
Perennial ryegrass breeding in New Zealand: a dairy industry perspective.Crossref | GoogleScholarGoogle Scholar |

Lowe KF, Moss RJ, Bowdler TM, Martin FD (1998) Digestibility and degradability of dry matter and crude protein of four temperate and one tropical grass in a subtropical environment. In ‘Animal production in Australia’. pp. 149–152.

Lowe KF, Bowdler TM, Hume DE, Casey ND, Tapper BA (2008) The effect of endophyte on the performance of irrigated perennial ryegrasses in subtropical Australia. Australian Journal of Agricultural Research 59, 567–577.
The effect of endophyte on the performance of irrigated perennial ryegrasses in subtropical Australia.Crossref | GoogleScholarGoogle Scholar |

McDonald P, Edwards RA, Greenhalgh JFD, Morgan CA, Sinclair LA, Wilkinson RG (2011) Digestion. In ‘Animal nutrition’. pp. 156–191. (Pearson Education Limited: Essex, UK)

McDonnell RP, Staines MvH, Douglas ML, Auldist MJ, Jacobs JL, Wales WJ (2017) Rumen degradability characteristics of five starch-based concentrate supplements used on Australian dairy farms. Animal Production Science 57, 1512–1519.
Rumen degradability characteristics of five starch-based concentrate supplements used on Australian dairy farms.Crossref | GoogleScholarGoogle Scholar |

NRC (2001) ‘Nutrient requirements of dairy cattle.’ (National Academies Press: Washington, DC)

Ohlsson C, Houmoller LP, Weisbjerg MR, Lund P, Hvelplund T (2007) Effective rumen degradation of dry matter, crude protein and neutral detergent fibre in forage determined by near infrared reflectance spectroscopy. Journal of Animal Physiology and Animal Nutrition 91, 498–507.
Effective rumen degradation of dry matter, crude protein and neutral detergent fibre in forage determined by near infrared reflectance spectroscopy.Crossref | GoogleScholarGoogle Scholar | 17988354PubMed |

Ørskov ER, McDonald I (1979) The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. The Journal of Agricultural Science 92, 499–503.
The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage.Crossref | GoogleScholarGoogle Scholar |

Pembleton KG, Hills JL, Freeman MJ, McLaren DK, French M, Rawnsley RP (2016) More milk from forage: milk production, blood metabolites, and forage intake of dairy cows grazing pasture mixes and spatially adjacent monocultures. Journal of Dairy Science 99, 3512–3528.
More milk from forage: milk production, blood metabolites, and forage intake of dairy cows grazing pasture mixes and spatially adjacent monocultures.Crossref | GoogleScholarGoogle Scholar | 26923052PubMed |

Rogers MJ, Lawson A, Kelly KB (2017) Forage options for dairy farms with reduced water availability in the Southern Murray Darling Basin of Australia. Sustainability 9, 2369–2388.
Forage options for dairy farms with reduced water availability in the Southern Murray Darling Basin of Australia.Crossref | GoogleScholarGoogle Scholar |

Rugoho I, Gourley CJP, Hannah M, Jacobs J (2016) Nutritive composition, mineral concentration and dietary cation-anion difference of feeds used within grazing-based dairy farms in Australia. Animal Production Science 57, 858–876.
Nutritive composition, mineral concentration and dietary cation-anion difference of feeds used within grazing-based dairy farms in Australia.Crossref | GoogleScholarGoogle Scholar |

Salama H, Lösche M, Herrmann A, Gierus M, Loges R, Feuerstein U, Ingwersen B, Stelling D, Luesink W, Taube F (2012) Limited genotype- and ploidy-related variation in the nutritive value of perennial ryegrass (Lolium perenne L.). Acta Agriculturæ Scandinavica. Section B, Soil and Plant Science 62, 23–34.
Limited genotype- and ploidy-related variation in the nutritive value of perennial ryegrass (Lolium perenne L.).Crossref | GoogleScholarGoogle Scholar |

Steg A, van Straalen WM, Hindle VA, Wensink WA, Dooper FMH, Schils RLM (1994) Rumen degradation and intestinal digestion of grass and clover at two maturity levels during the season in dairy cows. Grass and Forage Science 49, 378–390.
Rumen degradation and intestinal digestion of grass and clover at two maturity levels during the season in dairy cows.Crossref | GoogleScholarGoogle Scholar |

Sun XZ, Waghorn GC, Clark H (2010) Cultivar and age of regrowth effects on physical, chemical and in sacco degradation kinetics of vegetative perennial ryegrass (Lolium perenne L.). Animal Feed Science and Technology 155, 172–185.
Cultivar and age of regrowth effects on physical, chemical and in sacco degradation kinetics of vegetative perennial ryegrass (Lolium perenne L.).Crossref | GoogleScholarGoogle Scholar |

Tas BM, Taweel HZ, Smit HJ, Elgersma A, Dijkstra J, Tamminga S (2006) Rumen degradation characteristics of perennial ryegrass cultivars during the growing season. Animal Feed Science and Technology 131, 103–120.
Rumen degradation characteristics of perennial ryegrass cultivars during the growing season.Crossref | GoogleScholarGoogle Scholar |

Tedeschi LO, Galyean ML, Hales KE (2017) Recent advances in estimating protein and energy requirements of ruminants. Animal Production Science 57, 2237–2249.
Recent advances in estimating protein and energy requirements of ruminants.Crossref | GoogleScholarGoogle Scholar |

Wales WJ, Dellow DW, Doyle PT (1999) Degradabilities of dry matter and crude protein from perennial herbage and supplements used in dairy production systems in Victoria. Australian Journal of Experimental Agriculture 39, 645–656.
Degradabilities of dry matter and crude protein from perennial herbage and supplements used in dairy production systems in Victoria.Crossref | GoogleScholarGoogle Scholar |

Wales WJ, Heard JW, Ho CKM, Leddin CM, Stockdale CR, Walker GP, Doyle PT (2006) Profitable feeding of dairy cows on irrigated dairy farms in northern Victoria. Australian Journal of Experimental Agriculture 46, 743–752.
Profitable feeding of dairy cows on irrigated dairy farms in northern Victoria.Crossref | GoogleScholarGoogle Scholar |

Williams YJ, Doyle PT, Egan AR (2006) Rate of feed degradation in strip-grazing dairy cows. Australian Journal of Experimental Agriculture 46, 975–979.
Rate of feed degradation in strip-grazing dairy cows.Crossref | GoogleScholarGoogle Scholar |