Effect of carvacrol essential oils on growth performance and intestinal barrier function in broilers with lipopolysaccharide challenge
S. D. Liu
A B * , M. H. Song C * , W. Yun B , J. H. Lee B , H. B. Kim D E and J. H. Cho B E
A Department of Animal Science and Technology, Hebei Agricultural University, Baoding, 071000, China.
B Division of Food and Animal Science, Chungbuk National University, Cheongju-si, 361-763, Republic of Korea.
C Department of Animal Science and Biotechnology, Chungnam National University, Daejeon, 305-764, Republic of Korea.
D Department of Animal Resource and Science; Dankook University, Cheonan, 330-714, Republic of Korea.
E Corresponding author. Email: jinhcho@chungbuk.ac.kr; hbkim@dankook.ac.kr
Animal Production Science 60(4) 545-552 https://doi.org/10.1071/AN18326
Submitted: 23 May 2018 Accepted: 10 June 2019 Published: 22 January 2020
Abstract
The purpose of this study was to evaluate the effects of orally administrated carvacrol essential oils (CEOs) on the function of the intestinal barrier in broilers challenged by lipopolysaccharide (LPS). Eighty 28-day-old Ross 308 broilers were assigned to a 2 × 2 factorial arrangement of treatments (20 pens of 1 chick/treatment). Factors were CEOs (with or without orally administered) and LPS (challenged or non-challenged). Individually housed broilers were randomly assigned to four treatments (n = 20 broilers per treatment: 10 males and 10 females): no CEOs and no LPS challenge (NCEOs + NLPS); CEOs and no LPS challenge (CEOs + NLPS); no CEOs and LPS challenge (NCEOs + LPS); CEOs and LPS challenge (CEOs + LPS); all birds were fed the same diet. The broilers were administered 200 μL CEOs daily for 15 days. Orally administered CEOs significantly (P < 0.05) increased the gene expressions of occludin, claudin-1, claudin-5, zonula occludens-1 (ZO-1) and ZO-2 in the small intestinal mucosa, and increased (P < 0.05) the counts of goblet cell in the small intestinal epithelium; LPS-challenge damaged the intestinal barrier, and significantly (P < 0.05) reduced the gene expression of the aforementioned genes and increased the Escherichia coli (P < 0.05) and Salmonella enumeration (P < 0.05) in the intestine of broilers compared with the NCEOs + NLPS group. Injecting LPS reduced (P < 0.05) the gene expression of sIgA compared with the NCEOs + NLPS group. In summary, the CEOs improved the development of the intestinal barrier, to a certain extent, and repaired the intestinal damage caused by LPS.
Additional keywords: broiler, intestinal barrier, LPS, tight junction.
References
Anderson RC, Dalziel JE, Gopal PK, Bassett S, Ellis A, Roy NC (2012) The role of intestinal barrier function in early life in the development of colitis (IntechOpen). Available at https://www.intechopen.com/books/colitis/the-role-of-intestinal-barrier-function-in-early-life-in-the-development-of-colitis [Verified 16 June 2019]Ciftci M (2005) The effect of an essential oil mix derived from oregano, clove and anise on broiler performance. International Journal of Poultry Science 4, 444–451.
| The effect of an essential oil mix derived from oregano, clove and anise on broiler performance.Crossref | GoogleScholarGoogle Scholar |
Di Pasqua PR, Betts G, Hoskins N, Edwards M, Ercolini D, Mauriello G (2007) Membrane toxicity of antimicrobial compounds from essential oils. Journal of Agricultural and Food Chemistry 55, 4863–4870.
| Membrane toxicity of antimicrobial compounds from essential oils.Crossref | GoogleScholarGoogle Scholar |
Gnoth MJ, Clemens K, Evamaria KS, Silvia R (2000) Human milk oligosaccharides are minimally digested in vitro. Journal of Nutrition 130, 3014–3020.
| Human milk oligosaccharides are minimally digested in vitro.Crossref | GoogleScholarGoogle Scholar | 11110861PubMed |
González ML, Tapia R, Chamorro D (2008) Crosstalk of tight junction components with signaling pathways. Biochimica et Biophysica Acta (BBA) - Biomembranes 1778, 729–756.
| Crosstalk of tight junction components with signaling pathways.Crossref | GoogleScholarGoogle Scholar |
Gosain A, Gamelli RL (2005) Role of the gastrointestinal tract in burn sepsis. The Journal of Burn Care & Rehabilitation 26, 85–91.
| Role of the gastrointestinal tract in burn sepsis.Crossref | GoogleScholarGoogle Scholar |
Hayek SA, Aboghasem S, Mulumebet W, Salam AI (2013) Enzymatic activity of Lactobacillus reuteri grown in a sweet potato based medium with the addition of metal ions. SpringerPlus 2, 465
| Enzymatic activity of Lactobacillus reuteri grown in a sweet potato based medium with the addition of metal ions.Crossref | GoogleScholarGoogle Scholar | 24083112PubMed |
Kalemba D, Kunicka A (2003) Antibacterial and antifungal properties of essential oils. Current Medicinal Chemistry 10, 813–829.
| Antibacterial and antifungal properties of essential oils.Crossref | GoogleScholarGoogle Scholar | 12678685PubMed |
Khattak F, Ronchi A, Castelli P, Sparks N (2014) Effects of natural blend of essential oil on growth performance, blood biochemistry, cecal morphology, and carcass quality of broiler chickens. Poultry Science 93, 132–137.
| Effects of natural blend of essential oil on growth performance, blood biochemistry, cecal morphology, and carcass quality of broiler chickens.Crossref | GoogleScholarGoogle Scholar | 24570432PubMed |
Lee SA, Julian W, Helen V, Dawn VS, Emily JB, Sandra EH (2017) Understanding the direct and indirect mechanisms of xylanase action on starch digestion in broilers. Jounral of World Poulty Reserach 7, 35–47.
Li SY, Ru YJ, Liu M, Xu B, Péron A, Shi XG (2012) The effect of essential oils on performance, immunity and gut microbial population in weaner pigs. Livestock Science 145, 119–123.
| The effect of essential oils on performance, immunity and gut microbial population in weaner pigs.Crossref | GoogleScholarGoogle Scholar |
Liu SD, Song MH, Yun W, Lee JH, Lee CH, Kwak WG, Han NS, Kim HB, Cho JH (2018) Effects of oral administration of different dosages of carvacrol essential oils on intestinal barrier function in broilers. Journal of Animal Physiology and Animal Nutrition 102, 1257–1265.
| Effects of oral administration of different dosages of carvacrol essential oils on intestinal barrier function in broilers.Crossref | GoogleScholarGoogle Scholar |
McKay AM (1991) Extracellular β-galactosidase production during growth of filamentous fungi on polygalacturonic acid. Letters in Applied Miirohiology 12, 75–77.
| Extracellular β-galactosidase production during growth of filamentous fungi on polygalacturonic acid.Crossref | GoogleScholarGoogle Scholar |
National Research Council (1994) ‘Nutrient requirements of poultry.’ 9th rev. edn. (National Academy Press: Washington, DC)
Reisinger N, Steiner T, Nitsch S, Schatzmayr G, Applegate TJ (2011) Effects of a blend of essential oils on broiler performance and intestinal morphology during coccidial vaccine exposure. Journal of Applied Poultry Research 20, 272–283.
| Effects of a blend of essential oils on broiler performance and intestinal morphology during coccidial vaccine exposure.Crossref | GoogleScholarGoogle Scholar |
Sun Q, Liu D, Guo S, Chen Y, Guo Y (2015) Effects of dietary essential oil and enzyme supplementation on growth performance and gut health of broilers challenged by Clostridium perfringens. Animal Feed Science and Technology 207, 234–244.
| Effects of dietary essential oil and enzyme supplementation on growth performance and gut health of broilers challenged by Clostridium perfringens.Crossref | GoogleScholarGoogle Scholar |
Thompson KL, Applegate TJ (2006) Feed withdrawal alters small-intestinal morphology and mucus of broilers. Poultry Science 85, 1535–1540.
| Feed withdrawal alters small-intestinal morphology and mucus of broilers.Crossref | GoogleScholarGoogle Scholar | 16977838PubMed |
Tiihonen K, Kettunen H, Bento MH, Saarinen M, Lahtinen S, Ouwehand AC (2010) The effect of feeding essential oils on broiler performance and gut microbiota. British Poultry Science 51, 381–392.
| The effect of feeding essential oils on broiler performance and gut microbiota.Crossref | GoogleScholarGoogle Scholar | 20680873PubMed |
Tran AX, Whitfield C (2009) Lipopolysaccharides (endotoxins). Encyclopedia of Microbiology 71, 513–528.
| Lipopolysaccharides (endotoxins).Crossref | GoogleScholarGoogle Scholar |
Vicente Y, Da RC, Yu J, Hernandezperedo G, Martinez L, Pérezmies B (2001) Architecture and function of the gastroesophageal barrier in the piglet. Digestive Diseases and Sciences 46, 1899–1908.
| Architecture and function of the gastroesophageal barrier in the piglet.Crossref | GoogleScholarGoogle Scholar | 11575442PubMed |
