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Enzymatic hydrolysis drastically reduces fibre content of palm-kernel expeller, but without enhancing performance in broiler chickens

W. L. Chen A , M. F. Jahromi A B , S. C. L. Candyrine C , J. B. Liang A D , N. Abdullah A and Z. Idrus A
+ Author Affiliations
- Author Affiliations

A Institute of Tropical Agriculture and Food Security, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.

B Agriculture Biotechnology Research Institute of Iran (ABRII), East and North-East Branch, PO Box 91735/844, Mashhad, Iran.

C Faculty of Sustainable Agriculture, Universiti Malaysia Sabah, Sandakan Campus, 90509 Sandakan, Sabah, Malaysia.

D Corresponding author. Email: jbliang@upm.edu.my

Animal Production Science 59(12) 2131-2137 https://doi.org/10.1071/AN17860
Submitted: 13 October 2017  Accepted: 23 December 2017   Published: 21 February 2018

Abstract

The effectiveness of enzymatic hydrolysis of palm-kernel expeller (PKE) is dependent on various factors that influence the stability and functionality of the enzymes. In the present study, parameters influencing the enzymatic treatment of PKE were optimised employing response surface methodology. In addition, the effectiveness of enzymatic hydrolysed PKE in increasing inclusion rates in broiler diets was evaluated. Results showed that temperature, enzyme concentration and duration of hydrolysis had significant (P < 0.01) effects on the enzymatic hydrolysis of PKE. Using the crude enzyme produced by Aspergillus terreus K1 isolated in our laboratory, maximum reduction of crude fibre (40%) was achieved by fermenting the PKE at 60% initial moisture with 9.0 U/g PKE mannanase at 51°C for 18 h, with the production of 9.9% (w/w) of monosaccharides and oligosaccharides. Results of the growth-performance study indicated that inclusion rate of PKE with or without enzyme treatment in broiler diet is 5% for starter period and 20% for the finisher diet, without any detrimental effect on animal performance. Although the inclusion rate of enzyme-treated PKE can be increased to 30% without affecting average daily gain, feed conversion ratio of the birds will be compromised.

Additional keywords: broilers, feed-conversion efficiency, nutritive evaluation.


References

Abdollahi MR, Hosking BJ, Ning D, Ravindran V (2016) Influence of palm kernel meal inclusion and exogenous enzyme supplementation on growth performance, energy utilization, and nutrient digestibility in young broilers. Asian-Australasian Journal of Animal Sciences 29, 539–548.
Influence of palm kernel meal inclusion and exogenous enzyme supplementation on growth performance, energy utilization, and nutrient digestibility in young broilers.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2sXivVWrsLY%3D&md5=2908bf21371b8ed3e7ec1cb2c7a6e31eCAS |

Akpodiete OJ (2008) Performance response and egg qualities of laying birds fed enzyme supplemented palm kernel cake, PKC based diets. Animal Research International 5, 819–822.

Alshelmani MI, Loh TC, Foo HL, Lau WH, Sazili AQ (2014) Biodegradation of palm kernel cake by cellulolytic and hemicellulolytic bacterial cultures through solid state fermentation. The Scientific World Journal 2014, 729852
Biodegradation of palm kernel cake by cellulolytic and hemicellulolytic bacterial cultures through solid state fermentation.Crossref | GoogleScholarGoogle Scholar |

AOAC (1990) ‘Official methods of analysis.’ (Association of Official Analytical Chemists: Washington, DC)

Chen WL, Liang JB, Jahromi MF, Ho YW, Abdullah N (2013) Optimization of multi-enzyme production by fungi isolated from palm kernel expeller using response surface methodology. BioResources 8, 3844–3857.
Optimization of multi-enzyme production by fungi isolated from palm kernel expeller using response surface methodology.Crossref | GoogleScholarGoogle Scholar |

Chen WL, Liang JB, Jahromi MF, Abdullah N, Ho YW, Tufarelli V (2015) Enzyme treatment enhances release of prebiotic oligosaccharides from palm kernel expeller. BioResources 10, 196–209.

Cobb500 (2015) ‘Broiler performance and nutrition supplement.’ (Cobb-vantress Inc.: AR)

Deuel HJ, Hallman LF, Murray S, Hilllard J (1938) Studies on ketosis. 15. The comparative metabolism of d-mannose and d-glucose. The Journal of Biological Chemistry 125, 79–84.

Düsterhöft EM, Posthumus MA, Voragen AGJ (1992) Non-starch polysaccharides from sunflower, Helianthus annuus meal and palm-kernel, Elaeis guineenis meal-investigation of the structure of major polysaccharides. Journal of the Science of Food and Agriculture 59, 151–160.
Non-starch polysaccharides from sunflower, Helianthus annuus meal and palm-kernel, Elaeis guineenis meal-investigation of the structure of major polysaccharides.Crossref | GoogleScholarGoogle Scholar |

Esuga PM, Sekoni AA, Omage JJ, Bawa GS (2008) Evaluation of enzyme, Maxigrain® supplementation of graded levels of palm kernel meal, PKM on the performance of broiler chickens. Pakistan Journal of Biological Sciences 7, 607–613.

Hanafiah HA, Zulkifli I, Soleimani AF, Awad EA (2017) Apparent metabolisable energy and ileal crude protein digestibility of various treated palm kernel cake based diets for heat-stressed broiler chickens. Archiv für Geflügelkunde 81,
Apparent metabolisable energy and ileal crude protein digestibility of various treated palm kernel cake based diets for heat-stressed broiler chickens.Crossref | GoogleScholarGoogle Scholar |

Hooge DM, Pierce JL, McBride KW, Rigolin PJ (2010) Meta-analysis of broiler chicken trials using diets with or without Allzyme® SSF Enzyme Complex®. International Journal of Poultry Science 9, 819–823.
Meta-analysis of broiler chicken trials using diets with or without Allzyme® SSF Enzyme Complex®.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXht1eksLjI&md5=bc7bca74d82062a6fcb89c42859d5294CAS |

Jahromi MF, Liang JB, Abdullah N, Goh YM, Ebrahimi R, Shokryazdan P (2016) Extraction and characterization of oligosaccharides from palm kernel cake as prebiotic. BioResources 11, 674–695.

Kyriazakis I, Emmans GC (1995) The voluntary feed intake of pigs given feed based on wheat bran, dried citrus pulp and grass meal, in relation to measurements of feed bulk. British Journal of Nutrition 73, 191–207.
The voluntary feed intake of pigs given feed based on wheat bran, dried citrus pulp and grass meal, in relation to measurements of feed bulk.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2MXktFGltL4%3D&md5=2d05b4f082990c7e94646a84472f325fCAS |

Navidshad B, Liang JB, Jahromi MF, Akhlaghi A, Abdullah N (2015) A comparison between a yeast cell wall extract (Biol.-Mos®) and palm kernel expeller as mannan-oligosaccharides sources on the performance and ileal microbial population of broiler chickens. Italian Journal of Animal Science 14, 3452
A comparison between a yeast cell wall extract (Biol.-Mos®) and palm kernel expeller as mannan-oligosaccharides sources on the performance and ileal microbial population of broiler chickens.Crossref | GoogleScholarGoogle Scholar |

Navidshad B, Liang JB, Jahromi MF, Akhlaghi A, Abdullah N (2016) Effects of enzymatic treatment and shell content of palm kernel expeller meal on performance, nutrient digestibility, and ileal bacterial population in broiler chickens. Journal of Applied Poultry Research 25, 474–482.
Effects of enzymatic treatment and shell content of palm kernel expeller meal on performance, nutrient digestibility, and ileal bacterial population in broiler chickens.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC1cXivVWnt7k%3D&md5=67caacd37c6b1e1f78ebbe735c0c5c65CAS |

Ng WK, Lim HA, Lim SL, Ibrahim CO (2002) Nutritive value of palm kernel meal pretreated with enzyme or fermented with Trichoderma konningii (Oudemans) as dietary ingredients for red hybrid tilapia (Oreochromis sp.). Aquaculture Research 33, 1199–1207.
Nutritive value of palm kernel meal pretreated with enzyme or fermented with Trichoderma konningii (Oudemans) as dietary ingredients for red hybrid tilapia (Oreochromis sp.).Crossref | GoogleScholarGoogle Scholar |

Onifade AA, Babatunde GM (1998) Comparison of the utilisation of palm kernel meal, brewers’ dried grains and maize offal by broiler chicks. British Poultry Science 39, 245–250.
Comparison of the utilisation of palm kernel meal, brewers’ dried grains and maize offal by broiler chicks.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaK1czhtlGltw%3D%3D&md5=6e517a2b47872eda81245d8406ee245aCAS |

Rezaei K, Jenab E, Temelli F (2007) Effects of water on enzyme performance with an emphasis on the reactions in supercritical fluids. Critical Reviews in Biotechnology 27, 183–195.
Effects of water on enzyme performance with an emphasis on the reactions in supercritical fluids.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXhsVentr7P&md5=a0c46d42612ed7f6139d9558954e4640CAS |

Rezaei S, Jahromi MF, Liang JB, Zulkifli I, Farjam AS, Laudadio V, Tufarelli V (2015) Effect of oligosaccharides extract from palm kernel expeller on growth performance, gut microbiota and immune response in broiler chickens. Poultry Science 94, 2414–2420.
Effect of oligosaccharides extract from palm kernel expeller on growth performance, gut microbiota and immune response in broiler chickens.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2sXhvFGhtrjM&md5=9a1c086392aa303d784434254dc1b8e0CAS |

Saenphoom P, Liang JH, Loh TR (2011) Effect of enzyme treatment on chemical composition and production of reducing sugars in palm (Elaeis guineenis) kernel expeller. African Journal of Biotechnology 10, 15372–15377.
Effect of enzyme treatment on chemical composition and production of reducing sugars in palm (Elaeis guineenis) kernel expeller.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXhsFKhs7zJ&md5=4d50cfe4600a17011ba3fde96c718cf0CAS |

Saenphoom P, Liang JB, Ho YW, Loh TC, Rosfarizan M (2013) Effects of enzyme treated palm kernel expeller on metabolizable energy, growth performance, villus height and digesta viscosity in broiler chickens. Asian-Australasian Journal of Animal Sciences 26, 537–544.
Effects of enzyme treated palm kernel expeller on metabolizable energy, growth performance, villus height and digesta viscosity in broiler chickens.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXmvVSrt74%3D&md5=671dccc8bd7860d3b6c3055dcc2cd28dCAS |

Shakila S, Sudhakara Reddy P, Reddy PVVS, Ramana JV, Ravi A (2012) Effect of palm kernel meal on the performance of broilers. The Indian Journal of Animal Sciences 8, 227–234.

Soltan MA (2009) Growth performance, immune response and carcass traits of broiler chicks fed on graded level of palm kernel cake without or with enzyme supplementation. Livestock Research for Rural Development 21, 37

Sundu B, Dingle JG (2003) Use of enzymes to improve the nutritional value of palm kernel meal and copra meal. In ‘Queensland poultry science symposium 2003 (Vol. 11)’. (Ed. JG Dingle) pp. 1-15. (World’s Poultry Science Association: Queensland)

Sundu B, Kumar A, Dingle J (2006) Palm kernel meal in broiler diets, effect on chicken performance and health. World’s Poultry Science Journal 62, 316–325.
Palm kernel meal in broiler diets, effect on chicken performance and health.Crossref | GoogleScholarGoogle Scholar |

Swe KH, Alimon AR, Abdullah N, Jaafar MD (2003) True protein content of Aspergillus niger solid fermented PKC. In ‘Proceeding of the 25th of Malaysian Society of Animal Production’, 1–3 August, Melaka, Malaysia. pp. 127–128.

Wilson TH, Vincent TN (1955) Absorption of sugars in vitro by the intestine of the golden hamster. The Journal of Biological Chemistry 216, 851–866.