In-vitro assessment of antioxidant and antimicrobial activity of whole porcine-liver hydrolysates and its fractions
Akhilesh K. Verma A B , Manish Kumar Chatli A , Pavan Kumar A and Nitin Mehta A
+ Author Affiliations
- Author Affiliations
A Department of Livestock Products Technology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, (Punjab) India.
B Corresponding author. Email: vetakhilesh@gmail.com
Animal Production Science 59(4) 641-646 https://doi.org/10.1071/AN17047
Submitted: 25 January 2017 Accepted: 12 February 2018 Published: 26 April 2018
Abstract
Aim of the study was to evaluate antioxidant and antimicrobial activity of porcine liver-protein hydrolysates (PLHs) and their fractions recovered from enzymatic hydrolysis. Hydrolysates and fractions were assessed for antioxidants such as 2,2-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid, 2,2-diphenyl-1-picrylhydrazyl and ferric-reducing antioxidant power and antimicrobial activity. Inhibition activity for all antioxidant parameters was significantly (P < 0.05) higher for whole PLHs than for its corresponding fractions; however, among their fractions, 5–10 kDa and 1–5 kDa had a relatively higher antioxidant activity than did the other fractions. Trypsin-digested whole PLHs and their fractions exhibited the highest antioxidant and antimicrobial activity, followed by alcalase- and papain-digested PLHs, for all tested microbes. Results accomplished that whole PLHs exhibited better result than its fractions; thus, PLHs can be used as preservatives for food products or utilised for other pharmaceutical purposes.
Additional keywords: ABTS+, Bacillus cereus, DPPH, Escherichia coli, FRAP, Listeria monocytogenes, Staphylococcus aureus.
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