Effect of iron source on iron absorption by in situ ligated intestinal loops of broilers
Xiaofei Li A , Lingyan Zhang A , Liyang Zhang A , Lin Lu A B and Xugang Luo A B
+ Author Affiliations
- Author Affiliations
A Mineral Nutrition Research Division, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P. R. China.
B Corresponding author. Email: Lulin1225@163.com; wlysz@263.net
Animal Production Science 57(2) 308-314 https://doi.org/10.1071/AN15531
Submitted: 1 September 2015 Accepted: 3 November 2015 Published: 9 March 2016
Abstract
Two experiments were conducted to investigate the effect of iron (Fe) source on Fe absorption by in situ ligated intestinal loops of broilers. In Experiment 1, in situ ligated intestinal loops from Fe-deficient chicks (29 days old) were perfused with solutions containing 0.45 mmol Fe/L from FeSO4 (FeSO4·7H2O), Fe-Gly chelate, Fe-Met chelate, one of three Fe-amino acid or protein complexes with weak, moderate or extremely strong complex strength (Fe-Met W, Fe-Pro M, or Fe-Pro ES), or the mixtures of FeSO4 with either Gly or Met (Fe + Gly or Fe + Met), respectively, up to 30 min. In Experiment 2, in situ ligated duodenal loops from Fe-deficient chicks (29 days old) were perfused with solutions containing 0–3.58 mmol Fe/L from FeSO4, Fe-Met W, Fe-Pro M, or Fe-Pro ES up to 30 min. The absorptions of Fe from both inorganic and organic Fe sources in the ligated duodenum were ~1.35–2.8 times higher (P < 0.05) than that in the ligated jejunum or ileum. The absorption of Fe as Fe-Pro M or Fe-Pro ES was higher (P < 0.05) than that of Fe as inorganic Fe or Fe-Met W at Fe concentration of 3.58 mmol/L. The absorption kinetics of Fe from organic and inorganic Fe sources in the ligated duodenal loops followed a saturable process as determined by regression analysis of concentration-dependent absorption rates. The maximum absorption rate and Michaelis–Menten constant values in the ligated duodenal loops were higher (P < 0.05) for Fe-Pro M and Fe-Pro ES than for FeSO4 and Fe-Met W. The results from this study indicate that the duodenum was the main site of Fe absorption in the intestines of broilers; organic Fe sources with stronger complex strength values showed higher Fe absorptions at a higher concentration of added Fe; and the simple mixture of FeSO4 with amino acids did not increase Fe absorption.
Additional keywords: organic iron, small intestine.
References
Ashmead HD (1993) Comparative intestinal absorption and subsequent metabolism of metal amino acid chelates and inorganic metal salts. In ‘The roles of amino acid chelates in animal nutrition’. (Eds CB Ammerman, DH Baker, AJ Lewis) pp. 32–57. (Noyes Publications: Park Ridge, NJ)Ashmead HD (2001) The absorption and metabolism of iron amino acid chelate. Archivos Latinoamericanos de Nutricion 51, 13–21.
Ashmead HD, Graff DJ, Ashmead HH (1985) Summary and implications. In ‘Intestinal absorption of metal ions and chelates’. (Eds HD Ashmead) pp. 213–232. (Noyes Publications: Charles C. Thomas: Springfield, IL)
Bai SP, Lu L, Luo XG, Liu B (2008) Kinetics of manganese absorption in ligated small intestinal segments of broilers. Poultry Science 87, 2596–2604.
| Kinetics of manganese absorption in ligated small intestinal segments of broilers.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXmtVemtA%3D%3D&md5=59a995b3ce157a971c1651f7876b7f1bCAS | 19038816PubMed |
Bai SP, Lu L, Wang RL, Xi L, Zhang LY, Luo XG (2012) Manganese source affects manganese transport and gene expression of divalent metal transporter 1 in the small intestine of broilers. British Journal of Nutrition 108, 267–276.
| Manganese source affects manganese transport and gene expression of divalent metal transporter 1 in the small intestine of broilers.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XhtVamsrjL&md5=4873fdfe899c674f073575446f5402e9CAS | 22172096PubMed |
Condomina J, Zornoza-Sabina T, Granero L, Polache A (2002) Kinetics of zinc transport in vitro in rat small intestine and colon: interaction with copper. European Journal of Pharmaceutical Sciences 16, 289–295.
| Kinetics of zinc transport in vitro in rat small intestine and colon: interaction with copper.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38Xms1ynsrY%3D&md5=9038dc544c8b2eab309d340d841b8414CAS | 12208459PubMed |
Cook JD (1990) Adaptation in iron metabolism. The American Journal of Clinical Nutrition 51, 301–308.
Cook JD, Layrisse M, Martinez-Torres C, Walker R, Monsen E, Finch CA (1972) Food iron absorption measured by an extrinsic tag. The Journal of Clinical Investigation 51, 805–815.
| Food iron absorption measured by an extrinsic tag.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaE38XhsFagurY%3D&md5=69b2d20d9294355b77079b26de00ffe6CAS | 5062612PubMed |
Creech BL, Spears JW, Flowers WL, Hill GM, Lloyd KE, Armstrong TA, Engle TE (2004) Effect of dietary trace mineral concentration and source (inorganic vs. chelated) on performance, mineral status, and fecal mineral excretion in pigs from weaning through finishing. Journal of Animal Science 82, 2140–2147.
Duke GE (1984) Avian digestion. In ‘Duke’s physiology of domestic animals.’ (Ed. JS Melvin) pp. 359–366. (Cornell University Press: Ithaca, NY)
Gagne P, Dayton CM (2002) Best regression model using information criteria. Journal of Modern Applied Statistical Methods 5, 479–488.
Hempe JM, Cousins RJ (1989) Effect of EDTA and zinc-methionine complex on zinc absorption by rat intestine. The Journal of Nutrition 119, 1179–1187.
Holwerda RA, Albin RC, Madsen FC (1995) Chelation effectiveness of zinc proteinates demonstrated. Feedstuffs 67, 12–13.
Huang YL, Lu L, Li SF, Luo XG, Liu B (2009) Relative bioavailabilities of organic zinc sources with different chelation strengths for broilers fed a conventional corn-soybean meal diet. Journal of Animal Science 87, 2038–2046.
| Relative bioavailabilities of organic zinc sources with different chelation strengths for broilers fed a conventional corn-soybean meal diet.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXms1eisr4%3D&md5=79d2bd0153c5cfb52469f9a41294d906CAS | 19213702PubMed |
Huang YL, Lu L, Xie JJ, Li SF, Li XL, Liu SB, Zhang LY, Xi L, Luo XG (2013) Relative bioavailabilities of organic zinc sources with different chelation strengths for broilers fed diets with low or high phytate content. Animal Feed Science and Technology 179, 144–148.
| Relative bioavailabilities of organic zinc sources with different chelation strengths for broilers fed diets with low or high phytate content.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XhslGjsb7K&md5=553cca31b12083564501018bb65d358aCAS |
Ji F, Luo XG, Lu L, Liu B, Yu SX (2006a) Effect of manganese source on manganese absorption by the intestine of broilers. Poultry Science 85, 1947–1952.
| Effect of manganese source on manganese absorption by the intestine of broilers.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28Xht1Wjsb7P&md5=c2132be590725b79ba6d96fbaae5fcc5CAS | 17032828PubMed |
Ji F, Luo XG, Lu L, Liu B, Yu SX (2006b) Effects of manganese source and calcium on manganese uptake by in vitro everted gut sacs of broilers’ intestinal segments. Poultry Science 85, 1217–1225.
| Effects of manganese source and calcium on manganese uptake by in vitro everted gut sacs of broilers’ intestinal segments.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XntVyksbk%3D&md5=7fb0f64cddb6c82fe21f053919c74d9aCAS | 16830862PubMed |
Kwong RWM, Niyogi S (2008) An in vitro examination of intestinal iron absorption in a freshwater teleost, rainbow trout (Oncorhynchus mykiss). Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology 178, 963–975.
| An in vitro examination of intestinal iron absorption in a freshwater teleost, rainbow trout (Oncorhynchus mykiss).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXht1yqsrbJ&md5=b79538133293183d63456f511c8bce95CAS |
Li SF, Luo XG, Liu B, Crenshaw TD, Kuang X, Shao GZ, Yu SX (2004) Use of chemical characteristics to predict the relative bioavailability of supplemental organic manganese sources for broilers. Journal of Animal Science 82, 2352–2363.
Li SF, Luo XG, Lu L, Crenshaw TD, Bu YQ, Liu B, Kuang X, Shao GZ, Yu SX (2005) Bioavailability of organic manganese sources in broilers fed high dietary calcium. Animal Feed Science and Technology 123–124, 703–715.
| Bioavailability of organic manganese sources in broilers fed high dietary calcium.Crossref | GoogleScholarGoogle Scholar |
Li SF, Lu L, Hao SF, Wang YP, Zhang LY, Liu SB, Liu B, Li K, Luo XG (2011) Dietary manganese modulates expression of the manganese-containing superoxide dismutase gene in chickens. The Journal of Nutrition 141, 189–194.
| Dietary manganese modulates expression of the manganese-containing superoxide dismutase gene in chickens.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXhsVemuro%3D&md5=ddd0c5958508779e96ff645dec937b2cCAS |
Li XL, Xie JJ, Lu L, Zhang LY, Zhang LY, Zou YX, Wang QY, Luo XG, Li SF (2013) Kinetics of manganese transport and gene expressions of manganese transport carriers in Caco-2 cell monolayers. Biometals 26, 941–953.
| Kinetics of manganese transport and gene expressions of manganese transport carriers in Caco-2 cell monolayers.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhtlOisLrO&md5=99390d30b796b4acf156b79b7fa3e861CAS |
Ma WQ, Sun H, Zhou Y, Wu J, Feng J (2012) Effects of iron glycine chelate on growth, tissue mineral concentrations, fecal mineral excretion, and liver antioxidant enzyme activities in broilers. Biological Trace Element Research 149, 204–211.
| Effects of iron glycine chelate on growth, tissue mineral concentrations, fecal mineral excretion, and liver antioxidant enzyme activities in broilers.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XhsFSnsb3J&md5=d7fed1cbcf257ce21764397854f56de1CAS | 22549700PubMed |
Ma XY, Liu SB, Lu L, Li SF, Xie JJ, Zhang LY, Zhang JH, Luo XG (2014) Relative bioavailability of iron proteinate for broilers fed a casein-dextrose diet. Poultry Science 93, 556–563.
| Relative bioavailability of iron proteinate for broilers fed a casein-dextrose diet.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXjs1GjsLw%3D&md5=0243d8a0beff19c4e669080cdf79b8c7CAS | 24604848PubMed |
National Research Council (1994) ‘Nutrient requirements of poultry.’ 9th edn. (National Academy Press: Washington, DC)
Qiao YG, Tan BP, Mai KS, Ai QH, Zhang WB, Xu W (2013) Evaluation of iron methionine and iron sulphate as dietary iron sources for juvenile cobia (Rachycentron canadum). Aquaculture Nutrition 19, 721–730.
| Evaluation of iron methionine and iron sulphate as dietary iron sources for juvenile cobia (Rachycentron canadum).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhtlKrsbnJ&md5=9778b058d55818f435d26671f22897b1CAS |
SAS (2003) ‘SAS user’s guide, version 9.1.’ (SAS Institute Inc.: Cary, NC)
Schedl HP, Miller D, White D (1966) Use of polyethylene glycol and phenol red as unabsorbed indicators for intestinal absorption studies in man. Gut 7, 159–163.
| Use of polyethylene glycol and phenol red as unabsorbed indicators for intestinal absorption studies in man.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaF2sXoslA%3D&md5=7908b43e95769b804312ebf526e2aebdCAS | 4160440PubMed |
Seo SH, Lee HK, Lee WS, Shin KS, Paik IK (2008) The effect of level and period of Fe-methionine chelate supplementation on the iron content of boiler meat. Asian-Australasian Journal of Animal Sciences 21, 1501–1505.
| The effect of level and period of Fe-methionine chelate supplementation on the iron content of boiler meat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXhtlCisrbJ&md5=1854804889704f6fcbf46b3a501983d6CAS |
Steel L, Cousins RJ (1985) Kinetics of zinc absorption by luminally and vascularly perfused rat intestine. The American Journal of Physiology 248, G46–G53.
Sturkie P (2000) ‘Avian physiology.’ (Academic Press: San Diego, CA)
Tako E, Rutzke MA, Glahn RP (2010) Using the domestic chicken (Gallus gallus) as an in vivo model for iron bioavailability. Poultry Science 89, 514–521.
| Using the domestic chicken (Gallus gallus) as an in vivo model for iron bioavailability.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXktlyruro%3D&md5=13bb2687e679280fccb24e1944f02bb3CAS | 20181868PubMed |
Thomson ABR, Valberg LS (1971) Kinetics of intestinal iron absorption in the rat: effect of cobalt. The American Journal of Physiology 220, 1080–1085.
Thomson ABR, Valberg LS, Sinclair DG (1971) Competitive nature of the intestinal transport mechanism for cobalt and iron in the rat. The Journal of Clinical Investigation 50, 2384–2394.
| Competitive nature of the intestinal transport mechanism for cobalt and iron in the rat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaE38XjtVyqsQ%3D%3D&md5=c57e907eacd600c4009333b91242cfb6CAS |
Wang W, Di XM, D’Agostino RB, Torti SV, Torti FM (2007) Excess capacity of the iron regulatory protein system. The Journal of Biological Chemistry 282, 24650–24659.
| Excess capacity of the iron regulatory protein system.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXpt1SlsL0%3D&md5=df67ba6af43dc8fec946db098da876e6CAS | 17604281PubMed |
Wapnir RA, Khani DE, Bayne MA, Lifshitz F (1983) Absorption of zinc by the rat ileum: effects of histidine and other low-molecular-weight ligands. The Journal of Nutrition 113, 1346–1354.
Yang QM, Diao YX (2003) ‘The hand book for raising of broilers.’ (China Agriculture University Press: Beijing)
Yu Y, Lu L, Wang RL, Xi L, Luo XG, Liu B (2010) Effects of zinc source and phytate on zinc absorption by in situ ligated intestinal loops of broilers. Poultry Science 89, 2157–2165.
| Effects of zinc source and phytate on zinc absorption by in situ ligated intestinal loops of broilers.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXht12rurvI&md5=cd4c80eb26423de74fedd0d5b9a2f0f1CAS | 20852107PubMed |
