Effect of dietary fish oil with or without vitamin E supplementation on fresh and cryopreserved ovine sperm
M. Habibi A , M. J. Zamiri A D , A. Akhlaghi A , A. H. Shahverdi B , A. R. Alizadeh C and M. R. Jaafarzadeh A
+ Author Affiliations
- Author Affiliations
A Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, Iran.
B Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
C Department of Animal Science, Saveh Branch, Islamic Azad University, Saveh, Iran.
D Corresponding author. Email: zamiri@shirazu.ac.ir; mjzamiri@gmail.com
Animal Production Science 57(3) 441-447 https://doi.org/10.1071/AN15358
Submitted: 7 July 2015 Accepted: 4 December 2015 Published: 20 April 2016
Abstract
This study was carried out to determine whether dietary fish oil (FO) with or without vitamin E (VE) supplementation would have a beneficial effect on characteristics of fresh and cryopreserved ovine sperm. Sixteen rams were allotted to four groups and fed one of the four diets: control diet (CON; without FO and VE supplementation), VE diet supplying 200 IU VE/day.ram, FO diet containing 2.5% (dry matter basis) FO, and OVE diet containing FO and VE. Semen samples were collected at Weeks 7, 9 and 11 for evaluation of seminal quality. Frozen samples from Weeks 9 and 11 were subjected to computer-assisted semen analysis (CASA). Seminal volume, percentage of sperm with normal morphology and intact acrosome and seminal concentration of malondialdehyde were increased in FO rams (P < 0.05). In VE rams, semen volume and percentage of morphologically normal sperm were higher compared with CON rams; however, in OVE rams these attributes were not different from those in CON rams. Other traits in fresh semen were not affected by the diet. Fish oil alone negatively affected the sperm viability, but not motility, of the frozen–thawed semen; however, simultaneous supplementation with FO and VE resulted in increased percentage of morphologically normal sperm, motility, and viability. The percentages of CASA type A (showing rapid progressive motility) and type B (showing medium progressive motility) sperm were higher in OVE compared with other groups. Vitamin E supplementation decreased the beat-cross frequency values compared with other groups. Other CASA parameters were not affected by the diets. In conclusion, dietary FO and VE in the breeding season of ram, generally, do not cause significant changes in fresh sperm quality but, for cryopreservation of sperm, supplementation of the diet with an antioxidant such as VE may be beneficial when polyunsaturated fatty acids are added to the diet.
Additional keywords: ram, semen.
References
Adeel M, Ijaz A, Aleem M, Rehman H, Yousaf MS, Jabbar MA (2009) Improvement of liquid and frozen–thawed semen quality of Nili-Ravi buffalo bulls (Bubalus bubalis) through supplementation of fat. Theriogenology 71, 1220–1225.| Improvement of liquid and frozen–thawed semen quality of Nili-Ravi buffalo bulls (Bubalus bubalis) through supplementation of fat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXktF2nurs%3D&md5=9897e862761eccdf109d5a388cbbbea5CAS | 19246083PubMed |
Aitken RJ, Clarkson JS (1988) Significance of reactive oxygen species and antioxidants in defining the efficacy of spermatozoa preparation techniques. Journal of Andrology 9, 367–376.
Alizadeh AR, Esmaeili V, Shahverdi AH, Rashidi L (2014) Dietary fish oil can change sperm parameters and fatty acid profiles of ram sperm during oil consumption period and after removal of oil source. Cell Journal 16, 289–298.
Argov-Argaman N, Mahgrefthe K, Zeron Y, Roth Z (2013) Variation in lipid profiles within semen compartments – the bovine model of aging. Theriogenology 80, 712–721.
| Variation in lipid profiles within semen compartments – the bovine model of aging.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhtVKrsb%2FM&md5=39ae19abb0745f08949be1fc5000a184CAS | 23830232PubMed |
Attaman JA, Toth TL, Furtado J, Campos H, Hauser R, Chavarro JE (2012) Dietary fat and semen quality among men attending a fertility clinic. Human Reproduction (Oxford, England) 27, 1466–1474.
| Dietary fat and semen quality among men attending a fertility clinic.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38Xmt1Sltbk%3D&md5=e47e002cdc8eb409e43c00b41e52fe1bCAS |
Brinsko SP, Varner DD, Love CC, Blanchard TL, Day BC, Wilson ME (2005) Effect of feeding a DHA-enriched nutriceutical on the quality of fresh, cooled and frozen stallion semen. Theriogenology 63, 1519–1527.
| Effect of feeding a DHA-enriched nutriceutical on the quality of fresh, cooled and frozen stallion semen.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXhslWnurs%3D&md5=28a60f79281f218082f585602d82bf2dCAS | 15725455PubMed |
Castellano CA, Audet I, Bailey JL, Chouinard PY, Laforest JP, Matte JJ (2010) Effect of dietary n-3 fatty acids (fish oils) on boar reproduction and semen quality. Journal of Animal Science 88, 2346–2355.
| Effect of dietary n-3 fatty acids (fish oils) on boar reproduction and semen quality.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXos1Kmsbo%3D&md5=1c1be9b7c0d2e3ecf3bd7f9175524998CAS | 20348371PubMed |
Cerolini S, Kelso KA, Noble RC, Speak BK, Pizzi F, Cavalchini LG (1997) Relationship between spermatozoa lipid composition and fertility during aging of chickens. Biology of Reproduction 57, 976–980.
| Relationship between spermatozoa lipid composition and fertility during aging of chickens.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2sXmvFSis7k%3D&md5=6b4ea5774f0a7e8a72acd8f5b06ac92aCAS | 9369160PubMed |
Cerolini S, Zaniboni L, Maldjian A, Gliozzi T (2006) Effect of docosahexaenoic acid and α-tocopherol enrichment in chicken sperm on semen quality, sperm lipid composition and susceptibility to peroxidation. Theriogenology 66, 877–886.
| Effect of docosahexaenoic acid and α-tocopherol enrichment in chicken sperm on semen quality, sperm lipid composition and susceptibility to peroxidation.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28Xot1Wrtb0%3D&md5=f0c3975cd54aa49131e95f1049bb4ebeCAS | 16530814PubMed |
Chavarro JE, Furtado J, Toth TL, Ford J, Keller M, Campos H, Hauser R (2011) Trans-fatty acid levels in sperm are associated with sperm concentration among men from an infertility clinic. Fertility and Sterility 95, 1794–1797.
| Trans-fatty acid levels in sperm are associated with sperm concentration among men from an infertility clinic.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXjs1Srtbk%3D&md5=50cfb6121ce4836d4bc5da0f7e874bfdCAS | 21071027PubMed |
Chavarro JE, Minguez-Alarcon L, Mendiola J, Cutillas-Tolin A, Lopez-Eespin J, Torres-Cantero AM (2014) Trans fatty acid intake is inversely related to sperm count in young healthy men. Human Reproduction (Oxford, England) 29, 429–440.
| Trans fatty acid intake is inversely related to sperm count in young healthy men.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXis1Chtro%3D&md5=263787a9b13a0a0995dada53fe3b9eadCAS |
Combs GF, Jr (2008) ‘The vitamins: fundamental aspects in nutrition and health.’ 3rd edn. (Elsevier, Academic Press: New York)
Conquer JA, Martin JB, Tummon I, Watson L, Tekpetey F (2000) Effect of DHA supplementation on DHA status and sperm motility in asthenozoospermic males. Lipids 35, 149–154.
| Effect of DHA supplementation on DHA status and sperm motility in asthenozoospermic males.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXit1Krurc%3D&md5=16c2a4383be1e15f75015028223ea74bCAS | 10757545PubMed |
de Graaf SP, Peake K, Maxwell WMC, O’Brien JK, Evans G (2007) Influence of supplementing diet with oleic oleic and linoleic acid on the freezing ability and sex-sorting parameters of ram semen. Livestock Science 110, 166–173.
| Influence of supplementing diet with oleic oleic and linoleic acid on the freezing ability and sex-sorting parameters of ram semen.Crossref | GoogleScholarGoogle Scholar |
Dolatpanah MB, Towhidi A, Farshad A, Rashidi A, Rezayazdi A (2008) Effect of dietary fish oil on semen quality of goats. Asian-Australasian Journal of Animal Sciences 21, 29–34.
| Effect of dietary fish oil on semen quality of goats.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXktVWqurs%3D&md5=0dd8eb04d0b243035324057d1d0cb885CAS |
Esmaeili V, Shahverdi AH, Alizadeh AR, Alipour H, Towhidi A, Zarrabi M (2012) Fatty acid profile of ram’s sperm after removing some fatty acids from the diets and persistency of fatty acids in sperm. International Journal of Fertility & Sterility 5, 211–216.
Esmaeili V, Shahverdi AH, Alizadeh AR, Alipour H, Chehrazi M (2014) Saturated, omega-6 and omega-3 dietary fatty acid effects on the characteristics of fresh, frozen-thawed semen and blood parameters in rams. Andrologia 46, 42–49.
| Saturated, omega-6 and omega-3 dietary fatty acid effects on the characteristics of fresh, frozen-thawed semen and blood parameters in rams.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXovFyitQ%3D%3D&md5=7cfc1bde58b35a5f682ec91073317e4fCAS | 23157190PubMed |
Estienne MJ, Harper AF, Crawford RJ (2008) Dietary supplementation with a source of omega-3 fatty acids increases sperm number and the duration of ejaculation in boars. Theriogenology 70, 70–76.
| Dietary supplementation with a source of omega-3 fatty acids increases sperm number and the duration of ejaculation in boars.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXmvVymurg%3D&md5=dc402fbeafa4434ac0742d7ff672be21CAS | 18433852PubMed |
Evans G, Maxwell WMC (1987) ‘Salamon’s artificial insemination of sheep and goats.’ (Butterworths: Sydney)
Fair S, Doyle DN, Diskin MG, Hennessy AA, Kenny DA (2014) The effect of dietary n-3 polyunsaturated fatty acids supplementation of rams on semen quality and subsequent quality of liquid stored semen. Theriogenology 81, 210–219.
| The effect of dietary n-3 polyunsaturated fatty acids supplementation of rams on semen quality and subsequent quality of liquid stored semen.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhsFOgt7fO&md5=e0a39a908ea7ad1b415aa8e870074a90CAS | 24100164PubMed |
Gholami H, Chamani M, Towhidi A, Fazeli MH (2010) Effect of feeding a docosahexaenoic acid-enriched nutriceutical on the quality of fresh and frozen–thawed semen in Holstein bulls. Theriogenology 74, 1548–1558.
| Effect of feeding a docosahexaenoic acid-enriched nutriceutical on the quality of fresh and frozen–thawed semen in Holstein bulls.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXhtlKhsLnF&md5=20a913fb1f82bda2aee0f3eba5a316a0CAS | 20708237PubMed |
Gliozzi TM, Zaniboni L, Maldjian A, Luzi F, Maertens L, Cerolini S (2009) Quality and lipid composition of spermatozoa in rabbits fed DHA and vitamin E rich diets. Theriogenology 71, 910–919.
| Quality and lipid composition of spermatozoa in rabbits fed DHA and vitamin E rich diets.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXivVWltL0%3D&md5=add647c4cca9b904690a8a6fdbcb9fe7CAS | 19121864PubMed |
Gokcen H, Camas H, Erding H, Asti R, Cekgul E, Sener E (1990) Studies on the effects of vitamin E and selenium added to the ration on acrosome morphology, enzyme activity and fertility of frozen ram spermatozoa. Doga Turk Veterinerlik Ve Hayvancilik Dergisi 14, 207–218.
Gulliver CE, Friend MA, King BJ, Clayton EH (2012) The role of omega-3 polyunsaturated fatty acids in reproduction of sheep and cattle. Animal Reproduction Science 131, 9–22.
| The role of omega-3 polyunsaturated fatty acids in reproduction of sheep and cattle.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38Xjs1alu7Y%3D&md5=337de4b7a505d1f9f112a49af0dec926CAS | 22386690PubMed |
Hong Z, Hailing L, Hui M, Guijie Z, Leyan Y, Dubing Y (2010) Effect of vitamin E supplement in diet on antioxidant ability of testis in Boer goat. Animal Reproduction Science 117, 90–94.
| Effect of vitamin E supplement in diet on antioxidant ability of testis in Boer goat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXhtlyjt7nO&md5=ac5375bf8a7ab9ffcdcd442f181bd791CAS | 19427140PubMed |
Jafaroghli M, Abdi-Benemar H, Zamiri MJ, Khalili B, Farshad A, Shadparvar AA (2014) Effects of dietary n-3 fatty acids and vitamin C on semen characteristics, lipid composition of sperm and blood metabolites in fat-tailed Moghani rams. Animal Reproduction Science 147, 17–24.
| Effects of dietary n-3 fatty acids and vitamin C on semen characteristics, lipid composition of sperm and blood metabolites in fat-tailed Moghani rams.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXmtlCgtro%3D&md5=2efb91ea36ce091b843c3e3497802100CAS | 24745668PubMed |
Jenkins TC, Wallace RJ, Moate PJ, Mosley EE (2008) Board-invited review: recent advances in biohydrogenation of unsaturated fatty acids within the rumen microbial ecosystem. Journal of Animal Science 86, 397–412.
| Board-invited review: recent advances in biohydrogenation of unsaturated fatty acids within the rumen microbial ecosystem.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXhsVajsL4%3D&md5=d3cfb2c0d65f3132505119e5241abab8CAS | 18042812PubMed |
Jones R, Mann T, Sherins R (1979) Peroxidative breakdown of phospholipids in human spermatozoa, spermicidal properties of fatty acid peroxides, and protective action of seminal plasma. Fertility and Sterility 31, 531–537.
Kheradmand A, Babaei H, Abshenas J (2006) Comparative evaluation of the effect of antioxidants on the chilled-stored ram semen. Iranian Journal of Veterinary Research 7, 40–45.
Leedle RA, Leedle JA, Butine MD (1993) Vitamin E is not degraded by ruminal microorganisms: assessment with ruminal contents from a steer fed a high-concentrate diet. Journal of Animal Science 71, 3442–3450.
Maldjian A, Pizzi F, Gliozzi T, Cerolini S, Penny P, Noble R (2005) Changes in sperm quality and lipid composition during cryopreservation of boar semen. Theriogenology 63, 411–421.
| Changes in sperm quality and lipid composition during cryopreservation of boar semen.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXjsVSm&md5=5b5a3705bddd8185eeba85d50d8e6682CAS | 15626408PubMed |
Marin-Guzman J, Mahan DC, Chung YK, Pate JL, Pope WF (1997) Effects of dietary selenium and Vitamin vitamin E on boar performance and tissue responses, semen quality, and subsequent fertilization rates in mature gilts. Journal of Animal Science 75, 2994–3003.
Mitre R, Cheminade C, Allaume P, Lagrand P, Legrand A (2004) Oral intake of shark liver oil modifies lipid composition and improves motility and velocity of boar sperm. Theriogenology 62, 1557–1566.
| Oral intake of shark liver oil modifies lipid composition and improves motility and velocity of boar sperm.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXnvV2rtro%3D&md5=fc7598cace0a050ca15219fa980dab87CAS | 15451263PubMed |
NRC (2007) ‘Nutrient requirements of small ruminants: sheep, goats, cervids, and New World camelids.’ (National Research Council, National Academy Press: Washington, DC)
Paulenz H, Taugboslash O, Kommisrud E, Grevle IS (1999) Effect of dietary supplementation with cod liver oil on cold shock and freezability of boar semen. Reproduction in Domestic Animals 34, 431–435.
| Effect of dietary supplementation with cod liver oil on cold shock and freezability of boar semen.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1MXmvFWltr8%3D&md5=82f5f6fd3f26e1fa4dc05430c529c821CAS |
Pottier J, Focant M, Debier C, De Buysser G, Goffe C, Mignolet E, Froidmont E, Larondelle Y (2006) Effect of dietary vitamin E on rumen biohydrogenation pathways and milk fat depression in dairy cows fed high-fat diets. Journal of Dairy Science 89, 685–692.
| Effect of dietary vitamin E on rumen biohydrogenation pathways and milk fat depression in dairy cows fed high-fat diets.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XhtlWrtL8%3D&md5=4b40d980a4dc19edba580ff46d267544CAS | 16428637PubMed |
Revell SG, Mrode RA (1994) Anosmotic resistance test for bovine semen. Animal Reproduction Science 36, 77–86.
| Anosmotic resistance test for bovine semen.Crossref | GoogleScholarGoogle Scholar |
Roqueta-Rivera M, Abbott TL, Sivaguru M, Hess RA, Nakamura MT (2011) Deficiency in the omega-3 fatty acid pathway results in failure of acrosome biogenesis in mice. Biology of Reproduction 85, 721–732.
| Deficiency in the omega-3 fatty acid pathway results in failure of acrosome biogenesis in mice.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXht12ht73P&md5=e9dd2532f7620967e96c5352693a27ceCAS | 21653892PubMed |
Salamon S, Maxwell WMC (1995) Frozen storage of ram semen I. Processing, freezing, thawing and fertility after cervical insemination. Animal Reproduction Science 37, 185–249.
| Frozen storage of ram semen I. Processing, freezing, thawing and fertility after cervical insemination.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2MXltlertL0%3D&md5=6f82d921bb97e08091c5708f8477387dCAS |
Samadian F, Towhidi A, Rezayazdi K, Bahreini M (2010) Effects of dietary n-3 fatty acids on characteristics and lipid composition of ovine sperm. Animal 4, 2017–2022.
| Effects of dietary n-3 fatty acids on characteristics and lipid composition of ovine sperm.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXhtlGjsr%2FE&md5=9b7d68fbf8615edab1c30b4e89f29f03CAS | 22445376PubMed |
SAS (2003) ‘SAS/STAT user’s guide, Version 9.’ (Statistical Analysis System Institute Inc.: Cary, NC)
Selvaraju S, Raju P, Rao SBN, Raghavendra S, Nandi S, Dineshkumar D, Thayakumar A, Parthipan S, Ravindra JP (2012) Evaluation of maize grain and polyunsaturated fatty acid (PUFA) as energy sources for breeding rams based on hormonal, sperm functional parameters and fertility. Reproduction, Fertility and Development 24, 669–678.
| Evaluation of maize grain and polyunsaturated fatty acid (PUFA) as energy sources for breeding rams based on hormonal, sperm functional parameters and fertility.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XnvFehu78%3D&md5=cf3c11925d485db28dda8dc9939a2832CAS |
Sorensen AM, Jr (1979) ‘Animal reproduction.’ (McGraw-Hill Book Company: New York)
Surai PF, Noble RC, Sparks NHC, Speake BK (2000) Effect of long-term supplementation with arachidonic or docosahexaenoic acids on sperm production in the broiler chicken. Journal of Reproduction and Fertility 120, 2257–2264.
Tamuli MK, Watson PF (1994) Use of a simple staining technique to distinguish acrosomal changes in the live sperm sub-population. Animal Reproduction Science 35, 247–254.
| Use of a simple staining technique to distinguish acrosomal changes in the live sperm sub-population.Crossref | GoogleScholarGoogle Scholar |
Valk EE, Hornstra G (2000) Relationship between vitamin E requirement and polyunsaturated fatty acid intake in man: a review. International Journal for Vitamin and Nutrition Research 70, 31–42.
| Relationship between vitamin E requirement and polyunsaturated fatty acid intake in man: a review.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXktFWks7Y%3D&md5=12fbc2e77e15b56b3707383ddceb8f8cCAS | 10804454PubMed |
Wathes DC, Abayasekara DRE, Aitken RJ (2007) Polyunsaturated fatty acids in male and female reproduction. Biology of Reproduction 77, 190–201.
| Polyunsaturated fatty acids in male and female reproduction.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXot1OnsL0%3D&md5=fc3ebfba6db9849f953bbc2292fb354fCAS | 17442851PubMed |
Weiss WP (1998) Requirements of fat-soluble vitamins for dairy cows: a review. Journal of Dairy Science 81, 2493–2501.
| Requirements of fat-soluble vitamins for dairy cows: a review.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1cXmsVCrtrk%3D&md5=68517bcab8e2db479d29c384bff1ee02CAS | 9785241PubMed |
Weiss WP, Smith KL, Hogan JS, Steiner TE (1995) Effect of forage to concentrate ratio on disappearance of vitamins A and E during in vitro ruminal fermentation. Journal of Dairy Science 78, 1837–1842.
| Effect of forage to concentrate ratio on disappearance of vitamins A and E during in vitro ruminal fermentation.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2MXosVGmt7s%3D&md5=b44427865903ec6080955709ca6ca75aCAS | 8786267PubMed |
Wu SH, Oldfield JE, Whanger PD, Weswig PH (1973) Effect of selenium, vitamin E, and antioxidants on testicular function in rats. Biology of Reproduction 8, 625–629.
Yan L, Bai XL, Fang ZF, Che LQ, Xu SY, Wu D (2013) Effect of different dietary omega-3/omega-6 fatty acid ratios on reproduction in male rats. Lipids in Health and Disease 12, 33
| Effect of different dietary omega-3/omega-6 fatty acid ratios on reproduction in male rats.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXnvFajs7k%3D&md5=accaacd3f93ed81e73056414e8c1e7f4CAS | 23497018PubMed |
Yeste M, Barrera X, Coll D, Bonet S (2011) The effects on boar sperm quality of dietary supplementation with omega-3 polyunsaturated fatty acids differ among porcine breeds. Theriogenology 76, 184–196.
| The effects on boar sperm quality of dietary supplementation with omega-3 polyunsaturated fatty acids differ among porcine breeds.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXntFKitbc%3D&md5=1c8b641d1bea58363a9496c35504bda3CAS | 21458051PubMed |
Yue D, Yan L, Luo H, Xu X, Jin X (2010) Effect of vitamin E supplementation on semen quality and the testicular cell membranal and mitochondrial antioxidant abilities in Aohan fine-wool sheep. Animal Reproduction Science 118, 217–222.
| Effect of vitamin E supplementation on semen quality and the testicular cell membranal and mitochondrial antioxidant abilities in Aohan fine-wool sheep.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXhtVGlur8%3D&md5=e6f9d8e924658539f0d003014adc210bCAS | 19733455PubMed |
Zalata AA, Christophe AB, Depuydt CE, Schoonjans F, Comhaire FH (1998) The fatty acid composition of phospholipids of spermatozoa from infertile patients. Molecular Human Reproduction 4, 111–118.
| The fatty acid composition of phospholipids of spermatozoa from infertile patients.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1cXitlamurY%3D&md5=8d965bf469a67c61a35532db0e746924CAS | 9542967PubMed |
Zaniboni L, Rizzi R, Cerolini S (2006) Combined effect of DHA and α-tocopherol enrichment on sperm quality and fertility in the turkey. Theriogenology 65, 1813–1827.
| Combined effect of DHA and α-tocopherol enrichment on sperm quality and fertility in the turkey.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XksVCksbo%3D&md5=a9d6a82112108d33917d67915afdb501CAS | 16298425PubMed |
