CAPN1 gene as a potential marker for growth performance and carcass characteristics in pigs
K. Ropka-Molik A C , E. Robert B , M. Tyra B , K. Piórkowska A , M. Oczkowicz A , M. Szyndler-Nędza B and A. Bereta B
+ Author Affiliations
- Author Affiliations
A Laboratory of Genomics, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, Poland.
B Department of Animal Genetics and Breeding, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, Poland.
C Corresponding author. Email: katarzyna.ropka@izoo.krakow.pl
Animal Production Science 57(6) 1014-1021 https://doi.org/10.1071/AN14999
Submitted: 15 December 2014 Accepted: 9 April 2016 Published: 11 July 2016
Abstract
The calcium-activated neutral proteinase 1 (CAPN1) gene, which encodes µ-calpain, is considered to be associated with the majority of meat-quality traits. The objective of the present study was to evaluate the effect of CAPN1 polymorphism on the most important slaughter, fattening and pork-quality traits. The genotype frequency of g.1429G>A polymorphism was estimated on 860 pigs represented by five breeds (Polish Landrace, Polish Large White, Pietrain, Duroc and Pulawska). In all breeds, the most frequent were pigs with an AA genotype (60–74%), with the exception of Duroc pigs where the largest number of GG animals was observed (14%). The results showed that g.1429G>A polymorphism significantly affected the number of days on test (P ≤ 0.05) and age at slaughter (P ≤ 0.01), the weight of ham and primary cuts (P ≤ 0.05, P ≤ 0.01 respectively), loin eye area (P ≤ 0.01) and average backfat thickness (P ≤ 0.05). The g.1429G>A single-nucleotide polymorphism showed no clear influence on complex pork-quality traits. At the present stage of research, the results obtained allow for the use of g.1429G>A polymorphism in CAPN1 gene as genetic marker in pig selection to improve several slaughter or fattening traits.
Additional keywords: µ-calpain, fattening trait, meat quality, pig, slaughter trait.
References
Casas E, White SN, Wheeler TL, Shackelford SD, Koohmaraie M, Riley DG, Chase CC, Johnson DD, Smith TPL (2006) Effects of calpastatin and µ-calpain markers in beef cattle on tenderness traits. Journal of Animal Science 84, 520–525.Cheong HS, Yoon DH, Park BL, Kim LH, Bae JS, Namgoong S, Lee HW, Han ChS, Kim JO, Cheong ICh, Shin HD (2008) A single nucleotide polymorphism in CAPN1 associated with marbling score in Korean cattle. BMC Genetics 9, 33
| A single nucleotide polymorphism in CAPN1 associated with marbling score in Korean cattle.Crossref | GoogleScholarGoogle Scholar | 18423040PubMed |
Chung HY, Davis ME, Hines HC, Wulf DM (1999) Effect of calpain proteolysis and calpain genotypes on meat tenderness of Angus bulls. Research and Reviews: Beef and Sheep 169–199.
Costello S, O’Doherty E, Troy DJ, Ernst CW, Kim KS, Stapleton P, Sweeney T, Mullen AM (2007) Association of polymorphisms in the calpain I, calpain II and growth hormone genes with tenderness in bovine M. longissimus lumborum. Meat Science 75, 551–557.
| Association of polymorphisms in the calpain I, calpain II and growth hormone genes with tenderness in bovine M. longissimus lumborum.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXhtlaqu7Y%3D&md5=6bf04c09f0e218d59259fb693e1abaa4CAS | 22064018PubMed |
Court MH, Michael H (2012) ‘Court’s (2005–2008) online calculator.’ (Tuft University) Available at http://goo.gl/1UElb4 [Verified 23 June 2016]
Gandolfi G, Pomponio L, Ertbjerg P, Karlsson AH, Nanni Costa L, Lametsch R, Russo V, Davoli R (2011) Investigation on CAST, CAPN1 and CAPN3 porcine gene polymorphisms and expression in relation to post-mortem calpain activity in muscle and meat quality. Meat Science 88, 694–700.
| Investigation on CAST, CAPN1 and CAPN3 porcine gene polymorphisms and expression in relation to post-mortem calpain activity in muscle and meat quality.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXltFyrs7w%3D&md5=4fa801f8aee2965a76ff151da98e2377CAS | 21450414PubMed |
Gill JL, Bishop SC, McCorquodale C, Wiliams JL, Wiener P (2009) Association of selected SNP with carcass and taste panel assessed meat quality traits in a commercial population of Aberdeen Angus-sired beef cattle. Genetics, Selection, Evolution. 41, 36
| Association of selected SNP with carcass and taste panel assessed meat quality traits in a commercial population of Aberdeen Angus-sired beef cattle.Crossref | GoogleScholarGoogle Scholar | 19555501PubMed |
Goll DE, Thompson VF, Taylor RG, Ouali A (1998) The calpain system and skeletal muscle growth. Canadian Journal of Animal Science 78, 503–512.
| The calpain system and skeletal muscle growth.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1MXhtlCht78%3D&md5=b9d8c15c152cc619dcbf9dfd8fb8e143CAS |
Hamm R (1986) Functional properties of the myofibrilar system and their measurement. In ‘Muscle as food’. (Ed. PJ Bechtel) pp. 135–199. (Academic Press: London)
Huff-Lonergan E, Lonergan SM (1999) Post mortem mechanisms of meat tenderization: The roles of the structural proteins and the calpain system. In ‘Quality attributes of muscle foods’. (Eds YL Xiong, CT Ho, F Shahidi) pp. 229–251. (Kluwer Academic/Plenum Publishers: New York)
Huff-Lonergan E, Lonergan SM (2005) Mechanisms of water-holding capacity of meat: the role of postmortem biochemical and structural changes. Meat Science 71, 194–204.
| Mechanisms of water-holding capacity of meat: the role of postmortem biochemical and structural changes.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXmt1Wiurc%3D&md5=43a67e5ed71cce9627435ac5f289e811CAS | 22064064PubMed |
Huff-Lonergan E, Mitsuhashi T, Beekman DD, Parrish FC, Olson DG, Robson RM (1996) Proteolysis of specific muscle structural proteins by µ-calpain at low pH and temperature is similar to degradation in postmortem bovine muscle. Journal of Animal Science 74, 993–1008.
Jánossy J, Ubezio P, Apáti Á, Magócsi M, Tompa P, Friedrich P (2004) Calpain as a multi-site regulator of cell cycle. Biochemical Pharmacology 67, 1513–1521.
| Calpain as a multi-site regulator of cell cycle.Crossref | GoogleScholarGoogle Scholar | 15041468PubMed |
Jennifer LG, Stephen CB, McCorquodale C (2009) Association of selected SNP with carcass and taste panel assessed meat quality traits in a commercial population of Aberdeen Angus-sired of cattle. Genetics, Selection, Evolution. 41, 36
Juszczuk-Kubiak E, Sakowski T, Flisikowski K, Wicinska K, Oprzadek J, Rosochacki SJ (2004) Bovine mu-calpain (capn1) gene: new snp within intron 14. Journal of Applied Genetics 45, 457–460.
Kemp CM, Sensky PL, Bardsley RG, Buttery PJ, Parr T (2010) Tenderness: an enzymatic view. Meat Science 84, 248–256.
| Tenderness: an enzymatic view.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXhsVGlt7bN&md5=78b3a8abb778620cf3fc1f5eed908395CAS | 20374783PubMed |
Melody JL, Lonergan SM, Rowe LJ, Huiatt TW, Mayes MS, Huff-Lonergan E (2004) Early postmortem biochemical factors influence tenderness and water-holding capacity of three porcine muscles. Journal of Animal Science 82, 1195–1205.
Oczkowicz M, Tyra M, Ropka-Molik M, Mucha A, Żukowski K (2012) Effect of IGF2 intron3–g.3072G>A on intramuscular fat (IMF) content in pigs raised in Poland. Livestock Science 149, 301–304.
| Effect of IGF2 intron3–g.3072G>A on intramuscular fat (IMF) content in pigs raised in Poland.Crossref | GoogleScholarGoogle Scholar |
Page BT, Casas E, Heaton MP, Cullen NG, Hyndman DL, Morris CA, Crawford AM, Wheeler TL, Koohmaraie M, Keele JW, Smith TPL (2002) Evaluation of single-nucleotide polymorphisms in CAPN1 for association with meat tenderness in cattle. Journal of Animal Science 80, 3077–3085.
Page BT, Casas E, Quaas RL, Thallman RM, Wheeler TL, Shackelford SD, Koohmaraie M, White SN, Bennett GL, Keele JW, Dikeman ME, Smith TP (2004) Association of markers in the bovine capn1 gene with meat tenderness in large crossbred populations that sample influential industry sires. Journal of Animal Science 82, 3474–3481.
Pinto LFB, Ferraz JBS, Pedrosa VB, Eler JP, Meirelles FV, Bonin MN, Rezende FM, Carvalho ME, Cucco DC, Silva RCG (2011) Single nucleotide polymorphisms in CAPN1 and leptin genes associated with meat color and tenderness in Nellore cattle. Genetics and Molecular Research 10, 2057–2064.
| Single nucleotide polymorphisms in CAPN1 and leptin genes associated with meat color and tenderness in Nellore cattle.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXhtlajsLzK&md5=46617e6d43385d642e2a9b1b72fbacaeCAS |
Ropka-Molik K, Bereta A, Tyra M, Różycki M, Piórkowska K, Szyndler-Nędza M, Szmatoła T (2014a) Association of calpastatin gene polymorphisms and meat quality traits in pig. Meat Science 97, 143–150.
| Association of calpastatin gene polymorphisms and meat quality traits in pig.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXltFCksrc%3D&md5=09ce9d0670af461cc6f09980fa742783CAS | 24583321PubMed |
Ropka-Molik K, Eckert R, Piórkowska K (2014b) A new polymorphisms in regulatory regions of porcine µ-calpain gene and their association with CAPN1 transcript abundance. Annals of Animal Science 14, 525–535.
| A new polymorphisms in regulatory regions of porcine µ-calpain gene and their association with CAPN1 transcript abundance.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXhsleksLzM&md5=8425b9ac81a6d09a25fa5b2473754692CAS |
Shi M, Gao X, Ren H, Yuan Z, Wu H, Li J, Zhang L, Gao H, Li J, Xu S (2011) Association analysis of CAPN1 gene variants with carcass and meat quality traits in Chinese native cattle. African Journal of Biotechnology 10, 17367–17371.
Sorimachi H, Ishiura S, Suzuki K (1997) Structure and physiological function of calpains. The Biochemical Journal 328, 721–732.
| Structure and physiological function of calpains.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1cXjtVKntA%3D%3D&md5=1427e00a5c7066e41c3ccc275057f9ffCAS | 9396712PubMed |
Szyndler-Nedza M, Tyra M, Blicharski T, Piórkowska K (2010) Effect of mutation in MC4R gene on carcass quality in Pulawska pig included in conservation breeding programme. Animal Science Papers and Reports 28, 37–45.
Tyra M, Żak G (2012) Analysis of relationships between fattening and slaughter performance of pigs and the level of intramuscular fat (IMF) in longissimus lumborum muscle. Annals of Animal Science 12, 169–178.
| Analysis of relationships between fattening and slaughter performance of pigs and the level of intramuscular fat (IMF) in longissimus lumborum muscle.Crossref | GoogleScholarGoogle Scholar |
Xin J, Li-Chun Z, Zhao-Zhi L, Xiao-Hui L, Hai-Guo J, Chang-Guo Y (2011) Association of polymorphisms in the calpain I gene with meat quality traits in Yanbian yellow cattle of China. Asian-Australasian Journal of Animal Sciences 24, 9–16.
| Association of polymorphisms in the calpain I gene with meat quality traits in Yanbian yellow cattle of China.Crossref | GoogleScholarGoogle Scholar |
Zhang ZR, Zhu Q, Jiang XS, Du HR (2007) Study on correlation between single nucleotide polymorphism of CAPN1 gene and muscle tenderness and carcass traits in chicken. Hereditas (Beijing) 29, 982–988.
| Study on correlation between single nucleotide polymorphism of CAPN1 gene and muscle tenderness and carcass traits in chicken.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXpvVKisr0%3D&md5=6bb9e088f4ab0bcb1bb9aee923dcd781CAS |
Zhang ZR, Liu YP, Jiang X, Du HR, Zhu Q (2008) Study on association of single nucleotide polymorphism of CAPN1 gene with muscle fiber and carcass traits in quality chicken populations. Journal of Animal Breeding and Genetics 125, 258–264.
| Study on association of single nucleotide polymorphism of CAPN1 gene with muscle fiber and carcass traits in quality chicken populations.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXhtVWmtrbI&md5=a074288121d8338f35f92281323e63a8CAS | 18727211PubMed |
