Determination of changes in bovine plasma and milk proteins during naturally occurring Escherichia coli mastitis by comparative proteomic analysis
Yongxin Yang A , Suizhong Cao B C , Xiaowei Zhao A , Dongwei Huang A , Huiling Zhao A and Guanglong Cheng A D
+ Author Affiliations
- Author Affiliations
A Institute of Animal Science and Veterinary Medicine, Anhui Academy of Agricultural Sciences, Hefei 230031, China.
B College of Veterinary Medicine, Sichuan Agricultural University, Ya’an 625014, China.
C College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824, USA.
D Corresponding author. Email: cgl0126@126.com
Animal Production Science 56(11) 1888-1896 https://doi.org/10.1071/AN14901
Submitted: 27 July 2013 Accepted: 22 May 2015 Published: 15 September 2015
Abstract
The aim of this study was to investigate changes in plasma and milk proteins in response to Escherichia coli infection in cows. Plasma and milk were collected from healthy cows, cows suffering from mild E. coli mastitis, and cows suffering from severe E. coli mastitis. Protein composition was examined by two-dimensional gel electrophoresis coupled with mass spectrometry. Plasma haptoglobin and α-1 acid glycoprotein demonstrated greater expression in mastitic cows compared with controls, but there were no difference between mildly and severely mastitic cows. Milk from mildly mastitic cows showed increased albumin and casein variants. Severely mastitic cows showed lower casein levels and increased anti-microbial and acute phase proteins. Milk α-1 acid glycoprotein and cathelicidins were associated with severe mastitis. A greater number of β-casein fragments that corresponded to β-casein isoforms were found in milk from mildly mastitic cows. These results suggest that caseins levels decreased and the concentrations of anti-microbial and acute phase proteins increased corresponding to the degree of E. coli mastitis. Nevertheless, further studies are needed to determine whether cathelicidin could serve as a diagnostic marker for mastitis.
Additional keywords: dairy cows, proteome.
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