Use of n-alkanes for determination of Kikuyu grass (Pennisetum clandestinum) intake in free-range broilers
M. Singh A B D , T. Durali A B and A. J. Cowieson CA Poultry CRC, PO Box U242, Armidale, NSW 2351, Australia.
B Poultry Research Foundation, Faculty of Veterinary Science, University of Sydney, 425 Werombi Road, Camden, NSW 2570, Australia.
C DSM Nutritional Products, Orkney, KW17 2QT, United Kingdom.
D Corresponding author. Email: mini.singh@sydney.edu.au
Animal Production Science 56(7) 1152-1160 https://doi.org/10.1071/AN14778
Submitted: 25 August 2014 Accepted: 20 January 2015 Published: 22 April 2015
Abstract
N-alkanes, long-chained saturated hydrocarbons occurring in plant cuticles, were used for estimating grass intake in fast-growing, free-range broilers. A total of 1440 as hatched mixed sex Cobb 500 broilers were equally divided between one of four experimental treatments in a 2 by 2 factorial arrangement involving conventional (barn) or free-range (barn and range access) production systems and diets (crude protein: 21%, metabolisable energy: 13 MJ/K) with or without in-feed antibiotic (Zinc Bacitracin: Albac® G 150 antibiotic feed premix, Pfizer Australia Pty Ltd, Sydney, NSW, Australia). Day-old chicks were randomly allocated to 48 pens (12 replicates/treatment) with 30 birds in each pen. Chicks were assigned to treatment diets on Day 1 while free-range access was available to birds from Day 21 onwards. Alkane concentrations in litter were measured and compared with alkane profiles of the intake components (grass, diet pellets and woodchip). Correction for incomplete recovery followed by estimation using a non-negative least square procedure resulted in calculation of total grass intake from the range area. Kikuyu grass consumption was estimated to be 13.5–14.7% of total ‘as-fed’ intake, equating to 6.34–6.78 g of grass per bird per hour of range access in this study. Taking into account grass consumption, this resulted in a significant increase in feed intake by 8.7–8.9% (P < 0.01) and feed conversion ratio from 2.30 to 2.54 points (P < 0.01). It can be concluded that broilers reared under free-range conditions eat a substantial quantity of grass. However, the nutrient profile of grass is not complementary to the formulated ration and its consumption is likely to lead to an array of nutritional changes for the bird, thus affecting performance.
Additional keywords: broiler, free range, grass, n-alkanes.
References
Ali HAM, Mayes RW, Lamb CS, Hector BL, Verma AK, Orskov ER (2004) The potential of long-chain fatty alcohols and long-chain fatty acids as diet composition markers: development of methods for quantitative analysis and faecal recoveries of these compounds in sheep fed mixed diets. The Journal of Agricultural Science 142, 71–78.| The potential of long-chain fatty alcohols and long-chain fatty acids as diet composition markers: development of methods for quantitative analysis and faecal recoveries of these compounds in sheep fed mixed diets.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXlsFCjsL0%3D&md5=2d10fd579b68b91a392f6ff79846316bCAS |
Almeida GFD, Hinrichsen LK, Horsted K, Thamsborg SM, Hermansen JE (2012) Feed intake and activity level of two broiler genotypes foraging different types of vegetation in the finishing period. Poultry Science 91, 2105–2113.
| Feed intake and activity level of two broiler genotypes foraging different types of vegetation in the finishing period.Crossref | GoogleScholarGoogle Scholar |
Antell S, Ciszuk P (2006) Forage consumption of laying hens – the crop content as an indicator of feed intake and AME content of ingested forage. Archiv fur Geflugelkunde 70, 154–160.
Bassler A, Ciszuk P, Sjelin K (2000) Management of laying hens in mobile houses – a review of experiences. Ecological animal husbandry in the Nordic countries. In ‘NJF-seminar No. 303. Tjele, Denmark, 16–17 September 1999’. (Eds JE Hermansen, V Lund, E Thuen) pp. 45–50. (Danish Research Centre for Organic Research: Tjele, Denmark)
Buchanan NP, Hott JM, Kimbler LB, Moritz JS (2007) Nutrient composition and digestibility of organic broiler diets and pasture forages. Journal of Applied Poultry Research 16, 13–21.
| Nutrient composition and digestibility of organic broiler diets and pasture forages.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXls12itLk%3D&md5=b6e3d2858604077c2dfb1e3734547e24CAS |
Bugalho MN, Dove H, Kelman W, Wood JT, Mayes RW (2004) Plant wax alkanes and alcohols as herbivore diet composition markers. Journal of Range Management 57, 259–268.
| Plant wax alkanes and alcohols as herbivore diet composition markers.Crossref | GoogleScholarGoogle Scholar |
Ding LM, Long RJ (2010) The use of herbage N-alkanes as markers to estimate the diet composition of yaks on the Qinghai-Tibetan plateau. Asian-Australasian Journal of Animal Sciences 23, 61–67.
| The use of herbage N-alkanes as markers to estimate the diet composition of yaks on the Qinghai-Tibetan plateau.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXjvFCltr4%3D&md5=6f04c496e68d09d0b50c203b843ef8eaCAS |
Dove H (1992) Using the n-alkanes of plant cuticular wax to estimate the species composition of herbage mixtures. Australian Journal of Agricultural Research 43, 1711–1724.
| Using the n-alkanes of plant cuticular wax to estimate the species composition of herbage mixtures.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3sXnvFGmtg%3D%3D&md5=ad78da82bef2a0d03ff8d95d59ab4437CAS |
Dove H, Mayes R (1991) The use of plant wax alkanes as marker substances in studies of the nutrition of herbivores: a review. Australian Journal of Agricultural Research 42, 913–952.
| The use of plant wax alkanes as marker substances in studies of the nutrition of herbivores: a review.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3MXmsFCku78%3D&md5=c6bb0fd979308745c1f23122e7e352a0CAS |
Dove H, Mayes RW (1996) Plant wax components: a new approach to estimating intake and diet composition in herbivores. The Journal of Nutrition 126, 13–26.
Dove H, Mayes RW (2005) Using n-alkanes and other plant wax components to estimate intake, digestibility and diet composition of grazing/browsing sheep and goats. Small Ruminant Research 59, 123–139.
| Using n-alkanes and other plant wax components to estimate intake, digestibility and diet composition of grazing/browsing sheep and goats.Crossref | GoogleScholarGoogle Scholar |
Dove H, Moore AD (1995) Using a least-squares optimization procedure to estimate botanical composition based on the alkanes of plant cuticular wax. Australian Journal of Agricultural Research 46, 1535–1544.
| Using a least-squares optimization procedure to estimate botanical composition based on the alkanes of plant cuticular wax.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK28XhtFylsLY%3D&md5=43a6136f0d9e5572f87bf26e75468f95CAS |
Duncan AJ, Mayes RW, Lamb CS, Young SA, Castillo I (1999) The use of naturally occurring and artificially applied n-alkanes as markers for estimation of short-term diet composition and intake in sheep. The Journal of Agricultural Science 132, 233–246.
| The use of naturally occurring and artificially applied n-alkanes as markers for estimation of short-term diet composition and intake in sheep.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1MXivVGrtrY%3D&md5=9ec40072c9a343d82b802327c96e8f49CAS |
Durali T, Groves P, Cowieson AJ (2012) Comparison of performance of commercial conventional and free range broilers. In ‘Proceedings of the 23rd Annual Australian Poultry Science Symposium, Sydney, Australia, 19–22 February 2012’. pp. 28–31. (Faculty of Veterinary Science, University of Sydney: Sydney)
Elbe U, Ross A, Steffens G, Van den Weghe H, Winckler C (2004) Free range hens: interactions between use of outdoor run and amount of excrement in the outdoor run Freiland-Legehennen: Interaktionen zwischen Auslaufnutzung und Anfall von Ausscheidungen im Auslauf. In ‘Auf dem Weg zu einer tiergerechten Haltung, Wissenschaftliche Tagung 23–25 September 2004 an der Universitat fur Bodenkultur Wien, Vienna, Austria.’ pp. 75–78.
Ferreira LMM, Olivan M, Garcia U, Rodrigues MAM, Osoro K (2005) Validation of the alkane technique to estimate diet selection of goats grazing heather-gorse vegetation communities. Journal of the Science of Food and Agriculture 85, 1636–1646.
| Validation of the alkane technique to estimate diet selection of goats grazing heather-gorse vegetation communities.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXmsVaru7Y%3D&md5=ce7b040d20112fafdee662ddd6068a29CAS |
Ferreira LMM, Celaya R, Santos AS, Falco V, Guedes C, Rodrigues MAM, Osoro K (2011) The utilization of long-chain fatty acids as markers for diet composition estimates in ruminants: effects of animal species, diet composition and marker combination. Grass and Forage Science 66, 183–195.
| The utilization of long-chain fatty acids as markers for diet composition estimates in ruminants: effects of animal species, diet composition and marker combination.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXnvFOis74%3D&md5=cf2b7eb123bfaadb77c7476feac7dbb9CAS |
Ferreira LMM, Celaya R, Santos AS, Guedes CMV, Rodrigues MAM, Mayes RW, Osoro K (2012) Evaluation of long-chain alcohols as diet composition markers in goats grazing heathland areas. Animal 6, 683–692.
| Evaluation of long-chain alcohols as diet composition markers in goats grazing heathland areas.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38Xjt1eisrk%3D&md5=dc8f07e831c8cd1c9e6edbd8a0e0276fCAS |
FREPA (2011) FREPA RangeCare’s Code of Practice for Accreditation. 14. (Free Range Egg and Poultry Australia Ltd) Available at http://frepa.com.au/wp-content/uploads/2010/06/RangeCare-Code-of-Practice-for-Accrediation.pdf [Verified February 2015]
Glatz PC, Ru YJ, Miao ZH, Wyatt SK, Rodda BJ (2005) Integrating poultry into a crop and pasture farming system. International Journal of Poultry Science 4, 187–191.
| Integrating poultry into a crop and pasture farming system.Crossref | GoogleScholarGoogle Scholar |
Glatz PC, Miao ZH, Ru YJ (2006) Feeding free-range poultry and pigs. In ‘Feeding in domestic vertebrates: from structure to behaviour’. (Ed. V Bels) pp. 326–348. (CABI Publishing, CAB International: Wallingford, Oxfordshire, UK)
Hameleers A, Mcnab J, Mayes RW (1996) Use of aliphatic hydrocarbons (alkanes) as markers in nutrition studies in chickens. British Poultry Science 37, S55–S56.
Hatt JM, Mayes RW, Clauss M, Lechner-Doll M (2001) Use of artificially applied n-alkanes as markers for the estimation of digestibility, food selection and intake in pigeons (Columba livia). Animal Feed Science and Technology 94, 65–76.
| Use of artificially applied n-alkanes as markers for the estimation of digestibility, food selection and intake in pigeons (Columba livia).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXptVyht7g%3D&md5=54f08c31183f95607fceabefbc7114efCAS |
Horsted K, Hermansen JE, Hansen H (2007) Botanical composition of herbage intake of free-range laying hens determined by microhistological analysis of faeces. Archiv fur Geflugelkunde 71, 145–151.
IUPAC (1997) ‘Compendium of chemical terminology.’ 2nd edn (the ‘Gold Book’). (Blackwell Scientific Publications: Oxford, UK) Available at http://goldbook.iupac.org [Verified 8 July 2014]
Jondreville, C, Travel, A, Besnard, J, Feidt, C (2011) Intake of herbage and soil by free-range laying hens offered a complete diet compared to a whole-wheat diet Ingestion de sol et de vegetaux par des poules elevees en plein air nourries avec un aliment complet ou a base de ble entier et de coquilles marines. Neuviémes Journées de la Recherche Avicole, Tours, France, 29 et 30 mars 2011. pp. 91–95.
Jurjanz, S, Germain, K, Juin, H, Jondreville, C (2011) Ingestion of soil and plant matter by broilers in grassed or tree-planted outdoor runs Ingestion de sol et de vegetaux par le poulet de chair sur des parcours enherbes ou arbores. Neuviémes Journées de la Recherche Avicole, Tours, France, 29 et 30 mars 2011. pp. 101–105.
Jurjanz S, Germain K, Dziurla MA, Juin H, Jondreville C (2014) Use of acid-insoluble ash and n-alkanes as markers of soil and plant ingestion by chickens. Animal Feed Science and Technology 188, 92–101.
| Use of acid-insoluble ash and n-alkanes as markers of soil and plant ingestion by chickens.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXitVShu77M&md5=f49be3706180ffacf5243ab30bfbe189CAS |
Keir BL (2000) The potential use of urinary metabolites of plant compounds as markers for assessing the botanical composition of the diet of free-ranging herbivores. PhD Thesis, University of Aberdeen.
Kelman W, Bugalho M, Dove H (2003) Cuticular wax alkanes and alcohols used as markers to estimate diet composition of sheep (Ovis aries). Biochemical Systematics and Ecology 31, 919–927.
| Cuticular wax alkanes and alcohols used as markers to estimate diet composition of sheep (Ovis aries).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXktFGjsrs%3D&md5=611147ef5c8d0a9e9b1c9f5cfc0e0a83CAS |
Lazzari GL, Cossu ME, Cumini ML, Basilio AM (2007) Broiler productivity and carcass quality in a free range vs indoor systems. Productividad y calidad de carcasa en pollos parrilleros criados a parque vs confinamiento. Revista Argentina de Produccion Animal 27, 11–16.
Lin LJ, Zhu XY, Jiang C, Luo HL, Wang H, Zhang YJ, Hong FZ (2012) The potential use of n-alkanes, long-chain alcohols and long-chain fatty acids as diet composition markers: indoor validation with sheep and herbage species from the rangeland of Inner Mongolia of China. Animal 6, 449–458.
| The potential use of n-alkanes, long-chain alcohols and long-chain fatty acids as diet composition markers: indoor validation with sheep and herbage species from the rangeland of Inner Mongolia of China.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XhsFCitLo%3D&md5=e95881a952f6eaf93b89748b3a37904bCAS | 22436224PubMed |
Liu G, Wang S, Zhang Y, Han J, Liu GH, Wang SP, Zhang YJ, Han JG (2009) Estimation of diet composition and herbage intake of grazing cows using n-alkane technique in Inner Mongolia typical steppes. Acta Agrestia Sinica 17, 693–698.
MacLeod MG, Bentley JS (2012) Nutritional challenges of alternative production systems. In ‘Alternative systems for poultry: health, welfare and productivity’. (Eds V Sandilands, P Hocking) pp. 297–315. Poultry Science Symposium 30 (CAB International: Oxford, UK)
Mayes RW, Dove H (2000) Measurement of dietary nutrient intake in free-ranging mammalian herbivores. Nutrition Research Reviews 13, 107–138.
| Measurement of dietary nutrient intake in free-ranging mammalian herbivores.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXlvVaksbY%3D&md5=c3055f5ed44cc3b51cbf969844e287c0CAS | 19087435PubMed |
Mayes RW, Lamb CS, Colgrove PM (1986) The use of dosed and herbage n-alkanes as markers for the determination of herbage intake. The Journal of Agricultural Science 107, 161–170.
| The use of dosed and herbage n-alkanes as markers for the determination of herbage intake.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL28XlsFajs7k%3D&md5=b39f7d9e4c1c2668f7e27494c83bc3ebCAS |
Miao ZH, Glatz PC, Ru YJ (2005) Free-range poultry production – a review. Asian-Australasian Journal of Animal Sciences 18, 113–132.
| Free-range poultry production – a review.Crossref | GoogleScholarGoogle Scholar |
Mikulski D, Celej J, Jankowski J, Majewska T, Mikulska M (2011) Growth performance, carcass traits and meat quality of slower-growing and fast-growing chickens raised with and without outdoor access. Asian-Australasian Journal of Animal Sciences 24, 1407–1416.
| Growth performance, carcass traits and meat quality of slower-growing and fast-growing chickens raised with and without outdoor access.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXhsVOjsbjF&md5=b2b81cf39e5c1a03ff5d62b17691eac1CAS |
Milby TT (1961) Range versus confinement for growing turkeys. Poultry Science 40, 46–50.
| Range versus confinement for growing turkeys.Crossref | GoogleScholarGoogle Scholar |
Milošević N, Perić L, Strugar V, Žikić D, Pavlovski Z (2005) Rearing of fattening chickens on free range and extensively in chicken coop. Biotechnology in Animal Husbandry 21, 217–222.
Narvaez N, Brosh A, Pittroff W (2012) Use of n-alkanes to estimate seasonal diet composition and intake of sheep and goats grazing in California chaparral. Small Ruminant Research 104, 129–138.
| Use of n-alkanes to estimate seasonal diet composition and intake of sheep and goats grazing in California chaparral.Crossref | GoogleScholarGoogle Scholar |
Oliveira DEd, Silva SCd, de Oliveira DE, da Silva SC (2007) Alkanes as markers in nutritional studies with wild ruminant and non-ruminant animals. Scientia Agricola 64, 657–662.
| Alkanes as markers in nutritional studies with wild ruminant and non-ruminant animals.Crossref | GoogleScholarGoogle Scholar |
Pavlovski Z, Skrbic Z, Lukic M, Petricevic V, Trenkovski S (2009) The effect of genotype and housing system on production results of fattening chickens. Biotechnology in Animal Husbandry 25, 221–229.
| The effect of genotype and housing system on production results of fattening chickens.Crossref | GoogleScholarGoogle Scholar |
Peiretti PG, Meineri G, Miraglia N, Mucciarelli M, Bergero D (2006) Intake and apparent digestibility of hay or hay plus concentrate diets determined in horses by the total collection of feces and n-alkanes as internal markers. Livestock Science 100, 189–194.
| Intake and apparent digestibility of hay or hay plus concentrate diets determined in horses by the total collection of feces and n-alkanes as internal markers.Crossref | GoogleScholarGoogle Scholar |
Ponte PIP, Rosado CMC, Crespo JP, Crespo DG, Mourao JL, Chaveiro-Soares MA, Bras JLA, Mendes I, Gama LT, Prates JAM, Ferreira LMA, Fontes CMGA (2008a) Pasture intake improves the performance and meat sensory attributes of free-range broilers. Poultry Science 87, 71–79.
| Pasture intake improves the performance and meat sensory attributes of free-range broilers.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXhtVClur4%3D&md5=3bae8d847f9788bc574c0108b03c8637CAS |
Ponte PIP, Prates JAM, Crespo JP, Crespo DG, Mourao JL, Alves SP, Bessa RJB, Chaveiro-Soares MA, Gama LT, Ferreira LMA, Fontes CMGA (2008b) Restricting the intake of a cereal-based feed in free-range-pastured poultry: effects on performance and meat quality. Poultry Science 87, 2032–2042.
| Restricting the intake of a cereal-based feed in free-range-pastured poultry: effects on performance and meat quality.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXhtF2qt77I&md5=e1dcac9b8d1ea4fa3b6da3bc21ecad80CAS |
Premaratne S, Fontenot JP, Shanklin RK (2005) Use of N-alkanes to estimate intake and digestibility by beef steers. Asian-Australasian Journal of Animal Sciences 18, 1564–1568.
| Use of N-alkanes to estimate intake and digestibility by beef steers.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXht1KktLvM&md5=9f179bfbcaf903652db68da2200c7b71CAS |
Queiroz MFS, Morais JAS, Berchielli TT, Malheiros EB, Sader APO, Resende de KT, e Eifert EC (2011) Markers for estimate beef cattle digesta flow. Indicadores na estimativa do fluxo de digesta em bovinos de corte. Archivos de Zootecnia 60, 571–582.
| Markers for estimate beef cattle digesta flow. Indicadores na estimativa do fluxo de digesta em bovinos de corte.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38Xjs1amu7g%3D&md5=3899ef2e066883badebd44658d693f77CAS |
Rao SJ, Iason GR, Hulbert IAR, Mayes RW, Racey PA (2003) Estimating diet composition for mountain hares in newly established native woodland: development and application of plant-wax faecal markers. Canadian Journal of Zoology 81, 1047–1056.
| Estimating diet composition for mountain hares in newly established native woodland: development and application of plant-wax faecal markers.Crossref | GoogleScholarGoogle Scholar |
Rodriguez NM, Simoes Saliba EO, Guimaraes Junior R (2007) Use of indicators to estimate the consumption and digestibility of forage LIPE, purified enriched lignin. Uso de indicadores para estimar consumo y digestibilidad de pasto LIPE, lignina purificada y enriquecida. Revista Colombiana de Ciencias Pecuarias 20, 518–525.
Ru YJ, Kruk JA, Fischer M, Choct M, Glatz PC (2002) Predicting N-alkane concentration in pastures and deer faeces for dietary composition and digestibility measurement using near infrared spectroscopy. Asian-Australasian Journal of Animal Sciences 15, 1611–1616.
| Predicting N-alkane concentration in pastures and deer faeces for dietary composition and digestibility measurement using near infrared spectroscopy.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XpsV2mtb0%3D&md5=0c2bc4cf863542116e6351e899622a2dCAS |
Ru YJ, Hughes RJ, Choct M, Kruk JA (2003) Variation in nutritive value of commercial broiler diets. Asian-Australasian Journal of Animal Sciences 16, 830–836.
| Variation in nutritive value of commercial broiler diets.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXktF2gsr8%3D&md5=5121818305cfac35e12e0fbe5f8eead6CAS |
Takahashi SE, Mendes AA, Saldanha ESPB, Pizzolante CC, Pelicia K, Garcia RG, Paz ICLA, Quinteiro RR (2006) Effect of production system on performance and carcass yield of free range broiler chickens. Efeito do sistema de criacao sobre o desempenho e rendimento de carcaca de frangos de corte tipo colonial. Arquivo Brasileiro de Medicina Veterinária e Zootecnia 58, 624–632.
| Effect of production system on performance and carcass yield of free range broiler chickens. Efeito do sistema de criacao sobre o desempenho e rendimento de carcaca de frangos de corte tipo colonial.Crossref | GoogleScholarGoogle Scholar |
Walker A, Gordon S (2003) Intake of nutrients from pasture by poultry. The Proceedings of the Nutrition Society 62, 253–256.
| Intake of nutrients from pasture by poultry.Crossref | GoogleScholarGoogle Scholar | 14506872PubMed |
Ward JM, Houston DC, Ruxton GD, McCafferty DJ, Cook P (2001) Thermal resistance of chicken (Gallus domesticus) plumage: a comparison between broiler and free-range birds. British Poultry Science 42, 558–563.
| Thermal resistance of chicken (Gallus domesticus) plumage: a comparison between broiler and free-range birds.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD38%2FosV2msg%3D%3D&md5=3c6765f58095a8e2d7d0c60d59bda8adCAS | 11811905PubMed |