Effects of cloprostenol and flurogestone acetate sponge on embryo yields and quality in indigenous ewes in Bangladesh
B. F. Zohara A D ,A Department of Medicine, Surgery and Obstetrics, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh.
B Department of Medicine and Surgery, Chittagong Veterinary and Animal Sciences University, Chittagong, Bangladesh.
C Department of Surgery and Obstetrics, Bangladesh Agricultural University, Mymensingh, Bangladesh.
D Corresponding author. Email: begumfatemafatema@ymail.com
Animal Production Science 54(10) 1605-1608 https://doi.org/10.1071/AN14279
Submitted: 13 March 2014 Accepted: 14 June 2014 Published: 19 August 2014
Abstract
The effects of two doses of cloprostenol and two doses of flurogestone acetate sponge on the onset of oestrus, and embryo recovery and quality were evaluated. Thirty-two indigenous ewes (Wera breed) were allocated into four groups of eight. All ewes were synchronised with 100 µg (Group I) or 175 µg (Group II) cloprostenol injection, 9 days apart, or insertion of intravaginal sponges containing 30 mg (Group III) or 45 mg (Group IV) flurogestone acetate (FGA) for 12 days. The ewes were superovulated with 600 IU PMSG intramuscularly 10 days after the second cloprostenol injection or immediately after sponge removal on Day 12. After the detection of oestrus, the ewes were mated naturally at 6 and 12 h, and some ewes were inseminated laparoscopically. Embryos were recovered surgically 5 or 6 days after service. All ewes exhibited oestrus. The onset of oestrus occurred significantly (P < 0.05) earlier in FGA-treated (50.0 ± 1.5 and 48.0 ± 0.00 h) than in cloprostenol-treated groups. There were no significant differences (P > 0.05) in the mean time of onset of oestrus (50.0 ± 1.5 and 48.0 ± 0.00 h) between the two doses of cloprostenol. The mean number of corpora lutea (8.1 ± 1.26) and embryos recovered (6.1 ± 1.00) was significantly (P < 0.05) higher in ewes treated with 45 mg FGA than in ewes treated with cloprostenol. Embryo recovery rate was significantly (P < 0.05) higher in ewes treated with 45 mg FGA (75.4%) than in other groups (41.7% and 51.6% in 100 μg and 175 μg cloprostenol- and 52.7% in 30 mg FGA-treated groups, respectively). Fertilisation rate was 93.3% in ewes given 100 µg cloprostenol, whereas other groups showed 100% fertilisation rate. The highest percentage (100%) of Grade 1 embryos was in FGA groups. In conclusion, despite FGA protocol presenting superior results, cloprostenol protocol was equally efficient in synchronising oestrus. The embryo recovery rate was better after 45 mg FGA than 30 mg FGA or after either dose of cloprostenol.
Additional keywords: embryos, sheep, subtropical climate, superovulation, synchronisation.
References
Armstrong DT, Pfitzner AP, Warnes GM, Seamark RF (1983) Superovulation treatments and embryo transfer in Angora goats. Journal of Reproduction and Fertility 67, 403–410.| Superovulation treatments and embryo transfer in Angora goats.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaL3s7mtlyrsw%3D%3D&md5=29f57e2872f92d8caaa732310ff163a7CAS | 6403698PubMed |
Ataman MB, Akoz M, Akman O (2006) GnRH–PGF2α and PGF2α–PGF2α synchronization in Akkaraman cross-breed sheep in the breeding season. Bulletin of the Veterinary Institute Pulawy 50, 101–104.
Bari FY, Haresign W (1998) Embryo recovery, fertilization rates and embryo quality and survival rates in the superovulated ewes following natural service and/or laparoscopic intrauterine insemination. The Bangladesh Veterinarian 15, 1–6.
Bari FY, Khalid M, Haresign W, Murray A, Merrell B (2000) Effect of mating system, flushing procedure, progesterone dose and donor ewe age on the yield and quality of embryos within a MOET programme in sheep. Theriogenology 53, 727–742.
| Effect of mating system, flushing procedure, progesterone dose and donor ewe age on the yield and quality of embryos within a MOET programme in sheep.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD3c7pvFaitg%3D%3D&md5=521da545c95263d20bc71c66c7a2ff6fCAS |
Bari FY, Khalid M, Haresign W, Murray A, Richards RIW (2001) Effect of repeatability of treatment on superovulatory response and embryo recovery in sheep. Theriogenology 56, 147–155.
| Effect of repeatability of treatment on superovulatory response and embryo recovery in sheep.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD3MvhvV2quw%3D%3D&md5=75c7a3766189c7bdcbe5124133945417CAS |
Bari FY, Khalid M, Haresign W, Murray A, Merrell B (2003) Factors affecting the survival of sheep embryos after transfer within a MOET program. Theriogenology 59, 1265–1275.
| Factors affecting the survival of sheep embryos after transfer within a MOET program.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD3s%2FitFeqsw%3D%3D&md5=430a0791f338f5335f6a3531ac0e210cCAS |
Bettencourt EM, Bettencourt CM, Chagas e Silva J, Ferreira P, Manito CI, Matos CM, Romao RJ, Rocha A (2008) Effect of season and gonadotrophin preparation on superovulatory response and embryo quality in Portuguese Black Merinos. Small Ruminant Research 74, 134–139.
| Effect of season and gonadotrophin preparation on superovulatory response and embryo quality in Portuguese Black Merinos.Crossref | GoogleScholarGoogle Scholar |
Boland MP, Crosby TF, Gordon I (1983) Ovarian response in ewes following horse anterior pituitary extract and progestagen treatment. Animal Reproduction Science 6, 119–127.
| Ovarian response in ewes following horse anterior pituitary extract and progestagen treatment.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL2cXksF2lsQ%3D%3D&md5=643150e0bbce6f086f5dc026ad17ed8eCAS |
Caroline W, Summers PH (1999) The recovery rate of embryos using eight different protocols of synchronization and superovulation in sheep. Jurnal Ilmu Ternak dan Veteriner 4, 13–19.
Cordeiro MF, Lima-Verde JB, Lopes-Junior ES, Teixeira DIA, Farias LN, Salles HO, Simplicio AA, Rondina D, Freitas VJF (2003) Embryo recovery rate in Santa Ines ewes subjected to successive superovulatory treatments with p-FSH. Small Ruminant Research 49, 19–23.
| Embryo recovery rate in Santa Ines ewes subjected to successive superovulatory treatments with p-FSH.Crossref | GoogleScholarGoogle Scholar |
Gonzalez-Bulnes A, Veiga-Lopez A, Garcia P, Garcia-Garcia RM, Ariznavarreta C, Sanchez MA, Tresguerres JAF, Cocero MJ, Flores JM (2005) Effects of progestagens and prostaglandin analogues on ovarian function and embryo viability in sheep. Theriogenology 63, 2523–2534.
| Effects of progestagens and prostaglandin analogues on ovarian function and embryo viability in sheep.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXks1CktbY%3D&md5=253a813431fd2fb62544e299e0b26aa1CAS | 15910932PubMed |
Greyling JPC, Van Der Westhuysen JM (1980) The synchronization of oestrus in sheep. 3. The use of intravaginal progestagen and/or prostaglandin. South African Journal of Animal Science 10, 65–68.
Kor NM, Sadeghi S, Ziaei N (2012) Comparison reproductive Performance in Kermani ewes treated with two synchronization methods and subsequent eCG treatment out of the breeding season. International Journal of Biological & Medical Research 3, 1485–1489.
Macías-Cruz U, Álvarez-Valenzuela FD, Correa-Calderón A, Molina-Ramírez L, González-Reyna A, Soto-Navarro S, Avendaño-Reyes L (2009) Pelibuey ewe productivity and subsequent pre-weaning lamb performance using hair-sheep breeds under a confinement system. Journal of Applied Animal Research 36, 255–260.
| Pelibuey ewe productivity and subsequent pre-weaning lamb performance using hair-sheep breeds under a confinement system.Crossref | GoogleScholarGoogle Scholar |
Macías-Cruz U, Álvarez-Valenzuela FD, Olguín-Arredondo HA, Molina-Ramírez L, Avendaño-Reyes L (2012) Pelibuey ewes synchronized with progestagens and mated with rams from Dorper and Katahdin breed under feedlot conditions: ewe production and lamb growth during the pre-weaning period. Archivos de Medicina Veterinaria 44, 29–37.
Martinez-Tinajero JJ, Ruiz-Herluver I, Montanez-Valdez OD (2011) Reproductive performance in Dorper ewes synchronized at estrus during nonbreeding season in tropical conditions. Journal of Animal and Veterinary Advances 10, 221–223.
| Reproductive performance in Dorper ewes synchronized at estrus during nonbreeding season in tropical conditions.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XjvValtLk%3D&md5=36c385a1b858d7811759a6b564fff51eCAS |
Mayorga I, Mara L, Sanna D, Stelletta C, Morgante M, Casu S, Dattena M (2011) Good quality sheep embryos produced by superovulation treatment without the use of progesterone devices. Theriogenology 75, 1661–1668.
| Good quality sheep embryos produced by superovulation treatment without the use of progesterone devices.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXotFWgt7g%3D&md5=9b134aeab3cd5776a0fcb0c86a3728abCAS | 21396698PubMed |
Menchaca A, Vilariño M, Pinczak A, Kmaid S, Saldaña JM (2009) Progesterone treatment, FSH plus eCG, GnRH administration, and Day 0 Protocol for MOET programs in sheep. Theriogenology 72, 477–483.
| Progesterone treatment, FSH plus eCG, GnRH administration, and Day 0 Protocol for MOET programs in sheep.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXptl2nsrs%3D&md5=09ee49cf9c78da729b6cd70e26ca44abCAS | 19515409PubMed |
Naqvi SMK, Maurya VP, Gulyani R, Joshi A, Mittal JP (2004) The effect of thermal stress on superovulatory response and embryo production in Bharat Merino ewes. Small Ruminant Research 55, 57–63.
| The effect of thermal stress on superovulatory response and embryo production in Bharat Merino ewes.Crossref | GoogleScholarGoogle Scholar |
Oliveira MEF, D’Amato CC, Oliveira CS, Barros FFPC, Perini AP, Lima MR, Teixeira PPM, Feliciano MAR, Oliveira LG, Bicudo SD, Fonseca JF, Vicente WRR (2013) Effect of time of onset of follicle stimulating hormone treatment on superovulatory response and embryo yield of Santa Ines ewes during breeding season. Reproduction, Fertility and Development 25, 306
| Effect of time of onset of follicle stimulating hormone treatment on superovulatory response and embryo yield of Santa Ines ewes during breeding season.Crossref | GoogleScholarGoogle Scholar |
Pendleton RJ, Youngs CR, Rorei RW, Pool SH, Memon MA, Godke RA (1992) Follicle stimulating versus pregnant mare serum gonadotropin for superovulation of dairy goats. Small Ruminant Research 8, 217–224.
| Follicle stimulating versus pregnant mare serum gonadotropin for superovulation of dairy goats.Crossref | GoogleScholarGoogle Scholar |
Scudamore CL, Robinson JJ, Aitken RP, Robertson IS (1992) A comparison of two dosages of fluorogestone acetate in pessaries on the quality of embryos recovered from superovulated ewes. Theriogenology 37, 445–456.
| A comparison of two dosages of fluorogestone acetate in pessaries on the quality of embryos recovered from superovulated ewes.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK38XhvVyiu7Y%3D&md5=2c45fba62aadadef3af3265058c14590CAS |
Zonturlu AK, Ozyurtlu N, Kacar C (2011) Effect of different doses PMSG on estrus synchronization and fertility in Awassi ewes synchronized with progesterone during the transition period. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 17, 125–129.
