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Animal Production Science Animal Production Science Society
Food, fibre and pharmaceuticals from animals
RESEARCH ARTICLE

Bioactive forage legumes as a strategy to improve silage quality and minimise nitrogenous losses

G. Copani A , C. Ginane A , A. Le Morvan A and V. Niderkorn A B
+ Author Affiliations
- Author Affiliations

A INRA, UMR1213 Herbivores, F-63122 Saint-Genès-Champanelle, France.

B Corresponding author. Email: vincent.niderkorn@clermont.inra.fr

Animal Production Science 54(10) 1826-1829 https://doi.org/10.1071/AN14252
Submitted: 12 March 2014  Accepted: 30 June 2014   Published: 19 August 2014

Abstract

The use of forage legumes as a source of protein for ruminants is a sustainable strategy to reduce the use of inorganic-nitrogen fertiliser. In addition, some legumes species contain naturally bioactive secondary compounds, which could improve silage quality and digestive processes in ruminants. The aim of this study is to investigate the effects of bioactive legumes containing condensed tannins or polyphenol oxidase, ensiled alone or in mixture with a grass, on silage quality and conservation characteristics. Six mini-silos were prepared in triplicate as follows: 100% red clover (RC), 100% sainfoin (SF), 100% Timothy (T, control without bioactive compounds), binary mixtures 50% T + 50% RC, 50% T + 50% SF and ternary mixture 50% T + 25% RC + 25% SF. Condensed tannins remain unaltered during the silage-making process in terms of quantity and chemical structures, while polyphenol oxidase is activated during this process. All the silages that contained bioactive legumes were better conserved than the pure grass silo. In addition bioactive legumes were able to preserve protein from their degradation during the silage process. Inclusion of bioactive legumes can improve silage quality, and polyphenol oxidase may be more efficient than condensed tannins to improve the nitrogen value of silage.

Additional keywords: clovers, tannins.


References

Boudra H, Morgavi DP (2008) Reduction in Fusarium toxin levels in corn silage with low dry matter and storage time. Journal of Agricultural and Food Chemistry 56, 4523–4528.
Reduction in Fusarium toxin levels in corn silage with low dry matter and storage time.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXmt1Sru7s%3D&md5=800d6a464a32b42c42b7d2bdee464f20CAS | 18498169PubMed |

Bouwman L, Goldewijk KK, Van Der Hoe KW, Beusen AH, Van Vuuren DP, Willems J, Rufino MC, Stehfest E (2013) Exploring global changes in nitrogen and phosphorus cycles in agriculture induced by livestock production over the 1900–2050 period. In ‘Proceedings of the National Academy of Sciences’. pp. 20 882–20 887.

Conway EJ (1957) ‘Microdiffusion analysis and volumetric error.’

Cunniff P (1995) ‘Official methods of analysis of AOAC international.’ (Association of Official Analytical Chemists: Washington, DC)

Dulphy J, Demarquilly C (1981) Problèmes particuliers aux ensilages. In ‘Previsión de la valeur nutritive des aliments des ruminants’. p. 81. (INRA: Francia)

Gea A, Stringano E, Brown RH, Mueller-Harvey I (2011) In situ analysis and structural elucidation of sainfoin (Onobrychis viciifolia) tannins for high-throughput germplasm screening. Journal of Agricultural and Food Chemistry 59, 495–503.
In situ analysis and structural elucidation of sainfoin (Onobrychis viciifolia) tannins for high-throughput germplasm screening.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXhsF2ku7nJ&md5=14d7385e16a6c322321f1a283386d771CAS | 21175139PubMed |

Grabber JH, Coblentz W (2009) Polyphenol, conditioning, and conservation effects on protein fractions and degradability in forage legumes. Crop Science 49, 1511–1522.
Polyphenol, conditioning, and conservation effects on protein fractions and degradability in forage legumes.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXpvVyrt78%3D&md5=61244e02247dd6c9453435de0a5bc4cfCAS |

Grabber JH, Zeller WE, Mueller-Harvey I (2013) Acetone enhances the direct analysis of Procyanidin- and Prodelphinidin-based condensed tannins in Lotus species by the Butanol–HCl–Iron Assay. Journal of Agricultural and Food Chemistry 61, 2669–2678.
Acetone enhances the direct analysis of Procyanidin- and Prodelphinidin-based condensed tannins in Lotus species by the Butanol–HCl–Iron Assay.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhvFOlt74%3D&md5=356bb17535d84adea5b801fa8b2a718eCAS | 23383722PubMed |

Hetta M, Cone J, Gustavsson AM, Martinsson K (2003) The effect of additives in silages of pure timothy and timothy mixed with red clover on chemical composition and in vitro rumen fermentation characteristics. Grass and Forage Science 58, 249–257.
The effect of additives in silages of pure timothy and timothy mixed with red clover on chemical composition and in vitro rumen fermentation characteristics.Crossref | GoogleScholarGoogle Scholar |

Jouany JP (1982) Volatile fatty acid and alcohol determination in digestive contents, silage juices, bacterial cultures and anaerobic fermentor contents. Sciences des Aliments 2, 131–144.

Lee MRF, Scott MB, Tweed JKS, Minchin FR, Davies DR (2008) Effects of polyphenol oxidase on lipolysis and proteolysis of red clover silage with and without a silage inoculant (Lactobacillus plantarum L54). Animal Feed Science and Technology 144, 125–136.
Effects of polyphenol oxidase on lipolysis and proteolysis of red clover silage with and without a silage inoculant (Lactobacillus plantarum L54).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXntlansrw%3D&md5=ff4a1313a19724a633d43b324afb6e67CAS |

Lee MRF, Tweed JKS, Minchin FR, Winters AL (2009) Red clover polyphenol oxidase: activation, activity and efficacy under grazing. Animal Feed Science and Technology 149, 250–264.
Red clover polyphenol oxidase: activation, activity and efficacy under grazing.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXisVOksrk%3D&md5=87d1fc76eca5c1fb57ac246e8eea1570CAS |

Lee MRF, Cabiddu A, Hou F, Niderkorn V, Kim EJ, Fychan R, Scollan ND (2011) In vitro rumen simulated (RUSITEC) metabolism of freshly cut or wilted grasses with contrasting polyphenol oxidase activities. Grass and Forage Science 66, 196–205.
In vitro rumen simulated (RUSITEC) metabolism of freshly cut or wilted grasses with contrasting polyphenol oxidase activities.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXnvFOis78%3D&md5=25c43619c0ab794f49b6d3844eff8afdCAS |

Lüscher A, Mueller-Harvey I, Soussana J, Rees R, Peyraud J, Helgadóttir Á, Hopkins A (2013) Potential of legume-based grassland-livestock systems in Europe, the role of grasslands in a green future: threats and perspectives in less favoured areas. In ‘Proceedings of the 17th symposium of the European Grassland Federation’. pp. 3–29. (Agricultural University of Iceland: Akureyri)

Min BR, Barry TN, Attwood GT, McNabb WC (2003) The effect of condensed tannins on the nutrition and health of ruminants fed fresh temperate forages: a review. Animal Feed Science and Technology 106, 3–19.
The effect of condensed tannins on the nutrition and health of ruminants fed fresh temperate forages: a review.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXjtlSmtr0%3D&md5=85163c94b560f7d4ab1cbc8d47afad24CAS |

Niderkorn V, Baumont R (2009) Associative effects between forages on feed intake and digestion in ruminants. Animal 3, 951–960.
Associative effects between forages on feed intake and digestion in ruminants.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXhtVSnsL7K&md5=dd1281ac02c88b46f495ba3256f444feCAS | 22444815PubMed |

Rochon JJ, Doyle CJ, Greef JM, Hopkins A, Molle G, Sitzia M, Scholefield D, Smith CJ (2004) Grazing legumes in Europe: a review of their status, management, benefits, research needs and future prospects. Grass and Forage Science 59, 197–214.
Grazing legumes in Europe: a review of their status, management, benefits, research needs and future prospects.Crossref | GoogleScholarGoogle Scholar |

Scharenberg A, Arrigo Y, Gutzwiller A, Soliva CR, Wyss U, Kreuzer M, Dohme F (2007) Palatability in sheep and in vitro nutritional value of dried and ensiled sainfoin (Onobrychis viciifolia) birdsfoot trefoil (Lotus corniculatus), and chicory (Cichorium intybus). Archives of Animal Nutrition 61, 481–496.
Palatability in sheep and in vitro nutritional value of dried and ensiled sainfoin (Onobrychis viciifolia) birdsfoot trefoil (Lotus corniculatus), and chicory (Cichorium intybus).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXhtFyrurzM&md5=93414c8be14157a32d963a478f8a531fCAS | 18069619PubMed |

Theodoridou K, Aufrere J, Andueza D, Le Morvan A, Picard F, Pourrat J, Baumont R (2012) Effects of condensed tannins in wrapped silage bales of sainfoin (Onobrychis viciifolia) on in vivo and in situ digestion in sheep. Animal 6, 245–253.
Effects of condensed tannins in wrapped silage bales of sainfoin (Onobrychis viciifolia) on in vivo and in situ digestion in sheep.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38Xjs1agtw%3D%3D&md5=4350c6d03864cefe4a270297a41bef6cCAS | 22436182PubMed |

Van Soest PJ, Robertson JB, Lewis BA (1991) Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74, 3583–3597.
Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaK38%2FnvVCltA%3D%3D&md5=1b837d0361716b7c813fff797a7701e0CAS | 1660498PubMed |

Waghorn G (2008) Beneficial and detrimental effects of dietary condensed tannins for sustainable sheep and goat production – progress and challenges. Animal Feed Science and Technology 147, 116–139.
Beneficial and detrimental effects of dietary condensed tannins for sustainable sheep and goat production – progress and challenges.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXht1ejtL7J&md5=2dc659ef7f446ab749212f3b4afa8b43CAS |

Winters AL, Minchin FR, Michaelson-Yeates TPT, Lee MRF, Morris P (2008) Latent and active polyphenol oxidase (PPO) in Red Clover (Trifolium pratense) and use of a low PPO mutant to study the role of PPO in proteolysis reduction. Journal of Agricultural and Food Chemistry 56, 2817–2824.
Latent and active polyphenol oxidase (PPO) in Red Clover (Trifolium pratense) and use of a low PPO mutant to study the role of PPO in proteolysis reduction.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXjs1Oht7o%3D&md5=979952468113925bf32e2f4069430dedCAS | 18361497PubMed |