Possible involvement of myo-inositol in the physiological response of broilers to high doses of microbial phytase
A. J. Cowieson A C , R. Aureli B , P. Guggenbuhl B and F. Fru-Nji A
+ Author Affiliations
- Author Affiliations
A DSM Nutritional Products, Kaiseraugst, Switzerland.
B DSM Nutritional Products, CRNA, Village-Neuf, France.
C Corresponding author. Email: aaron.cowieson@dsm.com
Animal Production Science 55(6) 710-719 https://doi.org/10.1071/AN14044
Submitted: 27 January 2013 Accepted: 8 March 2014 Published: 20 May 2014
Abstract
The effect of high (1000–3000 phytase units (FYT)/kg) doses of microbial phytase on performance, nutrient digestibility and plasma inositol concentrations in young Ross broiler chicks was investigated in two separate experiments. In both experiments pelleted corn/soy-based diets were used and experimental duration was from Days 8 to 21 and Days 15 to 28 in Experiments 1 and 2, respectively. Treatments in Experiment 1 were arranged as a 2 × 4 + 1 factorial with two concentrations of calcium and available phosphorus and four concentrations of phytase (0, 1000, 2000 or 3000 FYT/kg), with a reference diet containing additional phosphorus and calcium from inorganic sources. In Experiment 2 only four dietary treatments were used, being a nutritionally adequate positive control, a negative control formulated to be insufficient in calcium and available phosphorus and the negative control supplemented with either 1000 or 2000 FYT/kg exogenous phytase. In both experiments, phytase improved performance relative to the appropriate control diet and increased the retention of calcium and phosphorus (P < 0.001). Tibia strength and ash content were increased (P < 0.001) by phytase addition. Plasma inositol concentrations were substantially increased (P < 0.001) by phytase addition to the diet. As inositol has been found to be an insulin mimetic in a range of animal species, these results suggest that part of the beneficial effect of high doses of phytase in broiler production may be conferred via insulin-like mechanisms. The effect of phytase on the expression of insulin-sensitive glucose transport systems, gluconeogenesis and nitrogen cycling is an area for future research. It can be concluded that phytase is effective in improving performance of broiler chicks fed diets that are sufficient and insufficient in calcium and phosphorus. Furthermore, phytase addition results in increased plasma inositol concentrations that may be beneficial in nutrient transport and protein deposition.
Additional keywords: glucose, insulin, nutrition, protein.
References
Bye JW, Cowieson NP, Cowieson AJ, Selle PH, Falconer RJ (2013) Dual effects of sodium phytate on the structural stability and solubility of proteins. Journal of Agricultural and Food Chemistry 61, 290–295.| Dual effects of sodium phytate on the structural stability and solubility of proteins.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XhvVKmsbzE&md5=1de78c24d7bec4abebfd0febf68d8ce5CAS | 23252762PubMed |
Cowieson AJ, Ravindran V (2007) Effect of phytic acid and microbial phytase on the flow and amino acid composition of endogenous protein at the terminal ileum of growing broiler chickens. The British Journal of Nutrition 98, 745–752.
| Effect of phytic acid and microbial phytase on the flow and amino acid composition of endogenous protein at the terminal ileum of growing broiler chickens.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXhtlWhsrjO&md5=98017e63feb50f2eb74b9c0536571403CAS | 17524177PubMed |
Cowieson AJ, Acamovic T, Bedford MR (2004) The effects of phytase and phytic acid on the loss of endogenous amino acids and minerals from broiler chickens. British Poultry Science 45, 101–108.
| The effects of phytase and phytic acid on the loss of endogenous amino acids and minerals from broiler chickens.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXhs1Wgu7c%3D&md5=0299e797fc7f69aaeec63b9b54ae5242CAS | 15115207PubMed |
Cowieson AJ, Wilcock P, Bedford MR (2011) Super-dosing effects of phytase in poultry and other monogastrics. World’s Poultry Science Journal 67, 225–236.
| Super-dosing effects of phytase in poultry and other monogastrics.Crossref | GoogleScholarGoogle Scholar |
Cowieson AJ, Ptak A, Mackowiak P, Sassek M, Prusznska-Oszmalek E, Zyla K, Swiatkiewicz S, Kacmarek S, Jozefiak D (2013) The effect of microbial phytase and myo-inositol on performance and blood biochemistry of broiler chickens fed wheat/corn-based diets. Poultry Science 92, 2124–2134.
| The effect of microbial phytase and myo-inositol on performance and blood biochemistry of broiler chickens fed wheat/corn-based diets.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXht1Ois7nK&md5=34ae91fd7da410bc4bc43c1d3c97b13dCAS | 23873561PubMed |
Dang NT, Mukai R, Yoshida K-I, Ashida H (2010) D-pinitol and myo-inositol stimulate translocation of glucose transporter 4 in skeletal muscle of C57BL/6 mice. Bioscience, Biotechnology, and Biochemistry 74, 1062–1067.
| D-pinitol and myo-inositol stimulate translocation of glucose transporter 4 in skeletal muscle of C57BL/6 mice.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXnt1Gmsb8%3D&md5=34c2ae16cb4bec7b7ea882778164a4d9CAS | 20460718PubMed |
Engelen AJ, van der Heeft FC, Randsdorp PHG, Smit ELC (1994) Simple and rapid determination of phytase activity. Journal of AOAC International 77, 730–764.
Goodgame SD, Mussini FJ, Lu C, Bradley SD, Waldroup PW (2011a) Effect of phytase on the sodium requirement of starting broilers. 1. Sodium chloride as the primary sodium source. International Journal of Poultry Science 10, 251–256.
| Effect of phytase on the sodium requirement of starting broilers. 1. Sodium chloride as the primary sodium source.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXmsFGqt7g%3D&md5=fba635189355d5c9ce8934ece0032086CAS |
Goodgame SD, Mussini FJ, Lu C, Bradley SD, Comert N, Waldroup PW (2011b) Effect of phytase on the sodium requirement of starting broilers. 2. Sodium bicarbonate as the primary sodium source. International Journal of Poultry Science 10, 766–773.
| Effect of phytase on the sodium requirement of starting broilers. 2. Sodium bicarbonate as the primary sodium source.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XlvFWjug%3D%3D&md5=fcceef2d769929a2c735275868937af8CAS |
Karadas F, Pirgozliev V, Pappas AC, Acamovic T, Bedford MR (2010) Effects of different dietary phytase activities on the concentration of antioxidants in the liver of growing broilers. Journal of Animal Physiology and Animal Nutrition 94, 519–526.
Leung K-Y, Mills K, Burren KA, Copp AJ, Greene NDE (2011) Quantitative analysis of myo-inositol in urine, blood and nutritional supplements by high-performance liquid chromatography tandem mass spectrometry. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences 879, 2759–2763.
| Quantitative analysis of myo-inositol in urine, blood and nutritional supplements by high-performance liquid chromatography tandem mass spectrometry.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXhtFehsLjP&md5=d246eca41146335a858d5fd371d36b93CAS | 21856255PubMed |
Nahapetian A, Young VR (1980) Metabolism of 14C-phytate in rats: effect of low and high dietary calcium intakes. The Journal of Nutrition 110, 1458–1472.
Olukosi OA, Cowieson AJ, Adeola O (2008) Energy utilization and growth performance of broilers receiving diets supplemented with enzymes containing carbohydrase or phytase activity individually or in combination. The British Journal of Nutrition 99, 682–690.
| Energy utilization and growth performance of broilers receiving diets supplemented with enzymes containing carbohydrase or phytase activity individually or in combination.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXivFeku7o%3D&md5=93883f7a9d1b32d2a67bbaea8ced6b0dCAS | 17761011PubMed |
Potgieter M, Pretorius E, Pepper MS (2013) Primary and secondary coenzyme Q10 deficiency: the role of therapeutic supplementation. Nutrition Reviews 71, 180–188.
| Primary and secondary coenzyme Q10 deficiency: the role of therapeutic supplementation.Crossref | GoogleScholarGoogle Scholar | 23452285PubMed |
Ravindran V, Morel PCH, Partridge GG, Hruby M, Sands JS (2006) Influence of an Eschericia coli-derived phytase on nutrient utilization in broiler starters fed diets containing varying concentrations of phytic acid. Poultry Science 85, 82–89.
| Influence of an Eschericia coli-derived phytase on nutrient utilization in broiler starters fed diets containing varying concentrations of phytic acid.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XltVKrsA%3D%3D&md5=3bff4a16f1a6dd98cdf6be4b752c99f6CAS | 16493949PubMed |
Ravindran V, Cowieson AJ, Selle PH (2008) Influence of dietary electrolyte balance and microbial phytase on growth performance, nutrient utilization and excreta quality of broiler chickens. Poultry Science 87, 677–688.
| Influence of dietary electrolyte balance and microbial phytase on growth performance, nutrient utilization and excreta quality of broiler chickens.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXkslyktb4%3D&md5=85e1121be4be4ef6816fcc072053ae16CAS | 18339988PubMed |
Selle PH, Ravindran V (2007) Microbial phytase in poultry nutrition. Animal Feed Science and Technology 135, 1–41.
| Microbial phytase in poultry nutrition.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXkt1artb8%3D&md5=1bd1b532a2930834b3981a9155c937b5CAS |
Selle PH, Ravindran V (2008) Phytate-degrading enzymes in pig nutrition. Livestock Science 113, 99–122.
| Phytate-degrading enzymes in pig nutrition.Crossref | GoogleScholarGoogle Scholar |
Selle PH, Cowieson AJ, Ravindran V (2009) Consequences of calcium interactions with phytate and phytase for poultry and pigs. Livestock Science 124, 126–141.
| Consequences of calcium interactions with phytate and phytase for poultry and pigs.Crossref | GoogleScholarGoogle Scholar |
Sweazea KL, Braun EJ (2006) Glucose transporter expression in English sparrows (Passer domesticus). Comparative Biochemistry and Physiology. Part B 144, 263–270.
Tamim NM, Angel R, Christman M (2004) Influence of dietary calcium and phytase on phytate phosphorus hydrolysis in broiler chickens. Poultry Science 83, 1358–1367.
| Influence of dietary calcium and phytase on phytate phosphorus hydrolysis in broiler chickens.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXntFGlsro%3D&md5=1112aec4d567249a02c92892b39a07e7CAS | 15339011PubMed |
Tokushima Y, Takahashi K, Sato K, Akiba Y (2005) Glucose uptake in vivo in skeletal muscles of insulin-injected chicks. Comparative Biochemistry and Physiology. Part B 141, 43–48.
VDLUFA (Verband Deutscher Landwirtschaftlicher Untersuchungs- und Forschungsanstalten Hrsg.) (1976) ‘Handbuch der landwirtschaftlichen Versuchs- und Untersuchungsmethodik (Methodenbuch) Band III: die chemische Untersuchung von Futtermitteln. 3.’ Auflage 1976 mit 1. Erg. Lief. 1983. 2. Erg. Lief. 1993. 3. Erg. Lief. 1997. (VDLUFA-Verlag: Darmstadt, Germany)
Wilkinson SJ, Selle PH, Bedford MR, Cowieson AJ (2014) Separate feeding of calcium improves performance and ileal nutrient digestibility in broiler chicks. Animal Production Science 54, 172–178.
| Separate feeding of calcium improves performance and ileal nutrient digestibility in broiler chicks.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXjtFOmsg%3D%3D&md5=680a47a45942e67fd64962ee16212830CAS |
Yamashita Y, Yamaoka M, Hasunuma T, Ashida H, Yoshida K-I (2013) Detection of orally administered inositol stereoisomers in mouse blood plasma and their effects on translocation of glucose transporter 4 in skeletal muscle cells. Journal of Agricultural and Food Chemistry 61, 4850–4854.
| Detection of orally administered inositol stereoisomers in mouse blood plasma and their effects on translocation of glucose transporter 4 in skeletal muscle cells.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXmvFSrtrk%3D&md5=5970e9e7066f112b4b2aae862bf147bdCAS | 23641877PubMed |
Zyla K, Dulinski M, Pierzchalska M, Grabacka M, Jozefiak D, Swiatkiewicz S (2013) Phytases and myo-inositol modulate performance, bone mineralization and alter lipid fractions in the serum of broilers. Journal of Animal and Feed Sciences 22, 56–62.
