Effects of two different forage sources on mammary gland growth, mammary cell turnover and activity in early lactation dairy cows
Tong Qin A , Haoyu Wang A , Dengpan Bu B , Haisheng Hao A , Dong Wang A and Huabin Zhu A CA Embryo Biotechnology and Reproduction Laboratory, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P.R. China.
B State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P.R. China.
C Corresponding author. Email: zhuhuabin@caas.cn
Animal Production Science 55(5) 630-633 https://doi.org/10.1071/AN13206
Submitted: 21 May 2013 Accepted: 19 February 2014 Published: 15 May 2014
Abstract
The objective of this study was to evaluate the effects of two different forage sources on mammary gland growth, mammary cell turnover and activity in early lactation dairy cows. Twelve early lactation cows were randomly assigned to a CS (33.8% corn straw as sole forage) or MF diet (3.7% Chinese wildrye + 28.4% alfalfa hay + 26.5% corn silage as mixed forage). All cows were fed from Week –3 to Week 8, and mammary biopsies were taken on 16 days postpartum. Mammary cell proliferation and apoptosis were determined by immunohistology, and genes expression in mammary were detected by real-time PCR. Results showed that cell proliferation, gene expression of milk proteins and proteins involved in the synthesis of milk components did not differ between two dietary treatments (P > 0.05). However, cows fed the MF diet had a higher IGF-1 receptor (IGF-1R) expression (P = 0.02), and lower rate of cell apoptosis (P = 0.003) relative to cows fed the CS diet. Collectively, these results suggest that the mammary secretory activity probably was not affected by the dietary treatments, but high quality and mixed forages led to the increased expression of IGF-1R and a larger number of cells in mammary glands, which may be responsible for the higher milk production in early lactation dairy cows.
Additional keywords: gene expression, proliferation and apoptosis, real-time qPCR.
References
Andersen CL, Jensen JL, Ørntoft TF (2004) Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Research 64, 5245–5250.| Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXmtF2mtbg%3D&md5=44d671a2fb9eeaf0bacb688e1e3f7dfbCAS | 15289330PubMed |
Bernier-Dodier P, Girard CL, Talbot BG, Lacasse P (2011) Effect of dry period management on mammary gland function and its endocrine regulation in dairy cows. Journal of Dairy Science 94, 4922–4936.
| Effect of dry period management on mammary gland function and its endocrine regulation in dairy cows.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXht1eisb7F&md5=2bac18d6df008e096e2e906163ef0f5fCAS | 21943744PubMed |
Breier BH, Sauerwein H (1995) Regulation of growth in ruminants by the somatotropic axis. In ‘Ruminant physiology: digestion, metabolism, growth and reproduction’. (Ed. P Cronje) pp. 102–123. (CABI Publishing: London)
Capuco AV, Wood DL, Baldwin R, McLeod K, Paape MJ (2001) Mammary cell number, proliferation, and apoptosis during a bovine lactation: relation to milk production and effect of bST. Journal of Dairy Science 84, 2177–2187.
| Mammary cell number, proliferation, and apoptosis during a bovine lactation: relation to milk production and effect of bST.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXnvV2ks7k%3D&md5=e58e8741d993e7d66961113ea2dcea7aCAS | 11699449PubMed |
Cisse M, Chilliard Y, Coxam V, Davicco MJ, Remond B (1991) Slow release somatotropin in dairy heifers and cows fed two levels of energy concentrate. 2. Plasma hormones and metabolites. Journal of Dairy Science 74, 1382–1394.
| Slow release somatotropin in dairy heifers and cows fed two levels of energy concentrate. 2. Plasma hormones and metabolites.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3MXlt1Olu7s%3D&md5=2c7976594d968600c77b5e7e2e51185fCAS | 1860980PubMed |
Colitti M, Stradaioli G, Stefanon B (2005) Mammary cell turnover in lactating ewes is modulated by changes of energy fuels. Research in Veterinary Science 78, 53–59.
| Mammary cell turnover in lactating ewes is modulated by changes of energy fuels.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD2crjtFymsQ%3D%3D&md5=2589df1bff77b86409c5b163b0f8b283CAS | 15500840PubMed |
Dessauge F, Lollivier V, Ponchon B, Bruckmaier R, Finot L, Wiart S, Cutullic E, Disenhaus C, Barbey S, Boutinaud M (2011) Effects of nutrient restriction on mammary cell turnover and mammary gland remodeling in lactating dairy cows. Journal of Dairy Science 94, 4623–4635.
| Effects of nutrient restriction on mammary cell turnover and mammary gland remodeling in lactating dairy cows.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXhtV2gsLfL&md5=3d20b3dc0bc0a277bad3f15943f30508CAS | 21854935PubMed |
Farr VC, Stelwagen K, Cate LR, Molenaar AJ, McFadden TB, Davis SR (1996) An improved method for the routine biopsy of bovine mammary tissue. Journal of Dairy Science 79, 543–549.
| An improved method for the routine biopsy of bovine mammary tissue.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK28Xjt1Ogurs%3D&md5=f45ab18cb27ee3d78188be2b0d986a30CAS | 8744218PubMed |
Gluckman PD, Breier BH, Davis SR (1987) Physiology of the somatotropic axis with particular reference to the ruminant. Journal of Dairy Science 70, 442–466.
| Physiology of the somatotropic axis with particular reference to the ruminant.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL2sXhvV2jtrk%3D&md5=43fea17338f1f9f24a377eb0c97f685dCAS | 3553258PubMed |
Knight CH, Peaker M (1982) Effects of fasting during mid pregnancy or early lactation on mammary development and milk yield in mice. The Journal of Dairy Research 49, 567–575.
| Effects of fasting during mid pregnancy or early lactation on mammary development and milk yield in mice.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaL3s%2FotlCktw%3D%3D&md5=a3d92ac6e170683d516324308977e908CAS | 6184388PubMed |
Nørgaard J, Sorensen A, Sorensen MT, Andersen JB, Sejrsen K (2005) Mammary cell turnover and enzyme activity in dairy cows: effects of milking frequency and diet energy density. Journal of Dairy Science 88, 975–982.
| Mammary cell turnover and enzyme activity in dairy cows: effects of milking frequency and diet energy density.Crossref | GoogleScholarGoogle Scholar | 15738232PubMed |
Plath-Gabler A, Gabler C, Sinowatz F, Berisha B, Schams D (2001) The expression of the IGF family and GH receptor in the bovine mammary gland. The Journal of Endocrinology 168, 39–48.
| The expression of the IGF family and GH receptor in the bovine mammary gland.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXotVGqtA%3D%3D&md5=21851bd39bc165bc5dca09111b48b731CAS | 11139768PubMed |
Qin T, Wang HY, Bu DP, Hao HS, Wang D, Du WH, Zhu HB (2013) Effect of a corn straw or mixed forage diet on endocrine, metabolism and lactation performance in periparturient cows. Journal of Animal and Veterinary Advances 12, 276–282.
Sejrsen K, Purup S (1997) Influence of prepubertal feeding level on milk yield potential of dairy heifers: a review. Journal of Dairy Science 75, 828–835. http://www.journalofanimalscience.org/content/75/3/828
Singh K, Erdman RA, Swanson KM, Molenaar AJ, Maqbool NJ, Wheeler TT, Arias JA, Quinn-Walsh EC, Stelwagen K (2010) Epigenetic regulation of milk production in dairy cows. Journal of Mammary Gland Biology and Neoplasia 15, 101–112.
| Epigenetic regulation of milk production in dairy cows.Crossref | GoogleScholarGoogle Scholar | 20131087PubMed |
Stefanon B, Colitti M, Gabai G, Knight CH, Wilde CJ (2002) Mammary apoptosis and lactation persistency in dairy animals. The Journal of Dairy Research 69, 37–52.
| Mammary apoptosis and lactation persistency in dairy animals.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XktlWrtLY%3D&md5=ae8128f6ec3a0c604182c7313fb37800CAS | 12047109PubMed |
Zhou Y, Akers RM, Jiang H (2008) Growth hormone can induce expression of four major milk protein genes in transfected MAC-T cells. Journal of Dairy Science 91, 100–108.
| Growth hormone can induce expression of four major milk protein genes in transfected MAC-T cells.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXhsVCrtQ%3D%3D&md5=c82b62dc49f34ea79061b4c569542d71CAS | 18096930PubMed |
