Polymorphisms of LHβ and GnRHR genes and their association with the number of embryos recovered in goats
M. Z. Fu A , G. Li B C and Z. Q. Zhou B
+ Author Affiliations
- Author Affiliations
A College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China.
B College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China.
C Corresponding author. Email: liguangdky@163.com
Animal Production Science 54(8) 987-991 https://doi.org/10.1071/AN13076
Submitted: 25 February 2013 Accepted: 1 October 2013 Published: 4 December 2013
Abstract
The objective of the present study was to explore a predictor of superovulation response on the basis of associations between the number of embryos recovered and gene polymorphism. Variation in the goat LHβ and GnRHR genes was investigated using polymerase chain reaction–single-strand conformational polymorphism and DNA sequencing. Two single nucleotide polymorphisms (SNPs) were identified in the 5′-UTR of LHβ gene (A59C, P1 locus) and in the Exon 2 of GnRHR gene (T177A, P6 locus). At the P1 locus in both breeds, the frequencies of one allele were 0.46 and 0.51, respectively. At the P6 locus, the minor allele frequency was 0.23. Associations of both SNPs with the number of embryos recovered and the corpus luteum number were evaluated in Boer and Shaanbei goat breeds. Association analysis showed that both SNPs had significant (P < 0.05) effects on the number of embryos recovered and corpus luteum number. These results indicate that LHβ and GnRHR genes are potential markers for the number of embryos recovered.
Additional keywords: goat, superovulation.
References
Althammer S, Pages A, Eyras E (2012) Predictive models of gene regulation from high-throughput epigenomics data. Comparative and Functional Genomics 2012, 284 786| Predictive models of gene regulation from high-throughput epigenomics data.Crossref | GoogleScholarGoogle Scholar |
An XP, Wang JG, Hou JX, Zhao HB, Bai L, Li G, Wang LX, Liu XQ, Xiao WP, Song YX, Cao BY (2011) Polymorphism identification in the goat MSTN gene and association analysis with growth traits. Czech Journal of Animal Science 56, 529–535.
Ayres SL, Gavin W, Memili E, Behboodi E (2012) Superovulation in goats during the second follicular wave, with or without exogenous progesterone. Small Ruminant Research 104, 146–150.
| Superovulation in goats during the second follicular wave, with or without exogenous progesterone.Crossref | GoogleScholarGoogle Scholar |
Beuzen ND, Stear MJ, Chang KC (2000) Molecular markers and their use in animal breeding. Veterinary Journal (London, England) 160, 42–52.
| Molecular markers and their use in animal breeding.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXmtVCjs70%3D&md5=f119c8ccb886bf523f232fd51e287d2dCAS |
Bó GA, Baruselli PS, Moreno D, Cutaia L, Caccia M, Tr’ıbulo R, Tr’ıbulo H, Mapletoft RJ (2002) The control of follicular wave development for self-appointed embryo transfer programs in cattle. Theriogenology 57, 53–72.
| The control of follicular wave development for self-appointed embryo transfer programs in cattle.Crossref | GoogleScholarGoogle Scholar | 11775981PubMed |
Brown P, Mcneilly JR, Wallace RM, Mcneilly AS, Clark AJ (1993) Characterization of the ovine LHβ-subunit gene: the promoter directs gonadotrope-specific expression in transgenic mice. Molecular and Cellular Endocrinology 93, 157–165.
| Characterization of the ovine LHβ-subunit gene: the promoter directs gonadotrope-specific expression in transgenic mice.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3sXkvVKksbc%3D&md5=29a8e99955e64f080b271099f712c87bCAS | 8349025PubMed |
Dunshea FR, Cronin GM, Barnett JL, Hemsworth PH, Hennessy DP, Campbell RG, Luxford B, Smits RJ, Tilbrook AJ, King RH, McCauley I (2011) Immunisation against gonadotrophin-releasing hormone (GnRH) increases growth and reduces variability in group-housed boars. Animal Production Science 51, 695–701.
| Immunisation against gonadotrophin-releasing hormone (GnRH) increases growth and reduces variability in group-housed boars.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXpvVOhtrc%3D&md5=2665bb6e711490c52b557c8d4a1ec287CAS |
Hasler JF (2003) The current status and future of commercial embryo transfer in cattle. Animal Reproduction Science 79, 245–264.
| The current status and future of commercial embryo transfer in cattle.Crossref | GoogleScholarGoogle Scholar | 14643107PubMed |
Hoffman GE, Le WW, Franceschini I, Caraty A, Advis JP (2011) Expression of fos and in vivo median eminence release of LHRH identifies an active role for preoptic area kisspeptin neurons in synchronized surges of LH and LHRH in the ewe. Endocrinology 152, 214–222.
| Expression of fos and in vivo median eminence release of LHRH identifies an active role for preoptic area kisspeptin neurons in synchronized surges of LH and LHRH in the ewe.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXitVCis7c%3D&md5=cc05983d070f38259e617d35ce46ac8aCAS | 21047947PubMed |
Kraus S, Naor ZS, Eger R (2001) Intracellular signaling pathways mediated by the gonadotropin-releasing hormone (GnRH) receptor. Archives of Medical Research 32, 499–509.
| Intracellular signaling pathways mediated by the gonadotropin-releasing hormone (GnRH) receptor.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XltVegtQ%3D%3D&md5=4817c6c50f0c2537ac217d241de218e9CAS | 11750725PubMed |
Lemieux C, Marguerat S, Lafontaine J, Barbezier N, Bahler J, Bachand F (2011) A pre-mRNA degradation pathway that selectively targets intron-containing genes requires the nuclear poly(A)-binding protein. Molecular Cell 44, 108–119.
| A pre-mRNA degradation pathway that selectively targets intron-containing genes requires the nuclear poly(A)-binding protein.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXht12gtrrJ&md5=f9da8b07099e61fd4ce0c14ceb2803e6CAS | 21981922PubMed |
Li G, Wu HP, Fu MZ, Zhou ZQ (2011) Novel single nucleotide polymorphisms of GnRHR gene and their association with litter size in goats. Archives of Animal Breeding 54, 618–624.
Mafra FA, Bianco B, Christofolini DM, Souza AMB, Zulli K, Barbosa CP (2010) Luteinizing hormone beta-subunit gene (LH beta) polymorphism in infertility and endometriosis-associated infertility. European Journal of Obstetrics, Gynecology, and Reproductive Biology 151, 66–69.
| Luteinizing hormone beta-subunit gene (LH beta) polymorphism in infertility and endometriosis-associated infertility.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXnsVaqs7o%3D&md5=d48b6f7664055e1542cb65d1a78017bfCAS | 20430510PubMed |
Mapletoft RJ, Steward KB, Adams JP (2002) Recent advances in the superovulation in cattle. Reproduction, Nutrition, Development 42, 601–611.
| Recent advances in the superovulation in cattle.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXit1ymtr8%3D&md5=76a2fc2c994a080f57b92fcbb5c484a4CAS | 12625424PubMed |
Merton JS, de Roos AP, Mullaart E, de Ruigh L, Kaal L, Vos PL, Dieleman SJ (2003) Factors affecting oocyte quality and quantity in commercial application of embryo technologies in the cattle breeding industry. Theriogenology 59, 651–674.
| Factors affecting oocyte quality and quantity in commercial application of embryo technologies in the cattle breeding industry.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD38jjslKrtA%3D%3D&md5=503fb1b8ff021b0968603e9b869253a6CAS | 12499010PubMed |
Moreau L, Charcosset A, Hospital F, Gallais A (1998) Marker-assisted selection efficiency in populations of finite size. Genetics 148, 1353–1365.
Mossa F, Duffy P, Naitana S, Lonergan P, Evans AC (2007) Association between numbers of ovarian follicles in the first follicle wave and superovulatory response in ewes. Animal Reproduction Science 100, 391–396.
| Association between numbers of ovarian follicles in the first follicle wave and superovulatory response in ewes.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXlvVOrtLw%3D&md5=09a5cbdecb76f70dd0de10fbfe8ed2bdCAS | 17101245PubMed |
Naor Z (2009) Signaling by G-protein-coupled receptor (GPCR): studies on the GnRH receptor. Frontiers in Neuroendocrinology 30, 10–29.
| Signaling by G-protein-coupled receptor (GPCR): studies on the GnRH receptor.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXhsVKntQ%3D%3D&md5=12d65adc791b948f8a3018646256f2e1CAS | 18708085PubMed |
Ramanujam LN, Liao WX, Roy AC, Loganath A, Goh HH, Ng SC (1999) Association of molecular variants of luteinizing hormone with menstrual disorders. Horumon To Rinsho 51, 243–246.
| Association of molecular variants of luteinizing hormone with menstrual disorders.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1MXlvFGls7o%3D&md5=0b72dbbacea49286c39f74c973130aa9CAS |
Ramanujam LN, Liao WX, Roy AC, Ng SC (2000) Association of molecular variants of luteinizing hormone with male infertility. Human Reproduction 15, 925–928.
| Association of molecular variants of luteinizing hormone with male infertility.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXivF2lt74%3D&md5=3b9fbc9d9d0f7195b55d7d43cff61907CAS | 10739843PubMed |
Smith JT, Li Q, Yap KS, Shahab M, Roseweir AK, Millar RP, Clarke IJ (2011) Kisspeptin is essential for the full preovulatory LH surge and stimulates GnRH release from the isolated ovine median eminence. Endocrinology 152, 1001–1012.
| Kisspeptin is essential for the full preovulatory LH surge and stimulates GnRH release from the isolated ovine median eminence.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXktVGrsbw%3D&md5=e56b54d39e2666c118a6e76763a47ee1CAS | 21239443PubMed |
Sorte H, Morkrid L, Rodningen O, Kulseth MA, Stray-Pedersen A, Matthijs G, Race V, Houge G, Fiskerstrand T, Bjurulf B, Lyle R, Prescott T (2012) Severe ALG8–CDG (CDG-Ih) associated with homozygosity for two novel missense mutations detected by exome sequencing of candidate genes. European Journal of Medical Genetics 55, 196–202.
| Severe ALG8–CDG (CDG-Ih) associated with homozygosity for two novel missense mutations detected by exome sequencing of candidate genes.Crossref | GoogleScholarGoogle Scholar | 22306853PubMed |
Stojilkovic SS, Reinhart J, Catt KJ (1994) Gonadotropin-releasing hormone receptors: structure and signal transduction pathways. Endocrine Reviews 15, 462–499.
Tabor HK, Risch NJ, Myers RM (2002) Candidate gene approaches for studying complex genetic traits: practical considerations. Nature Reviews. Genetics 3, 391–397.
| Candidate gene approaches for studying complex genetic traits: practical considerations.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38Xjs1yhtb4%3D&md5=2b773b9d08e416dabd28a4e8977579afCAS | 11988764PubMed |
Yang WC, Li SJ, Xie YH, Tang KQ, Hua GH, Zhang CY, Yang LG (2011a) Two novel SNPs of the type I gonadotropin releasing hormone receptor gene and their associations with superovulation traits in Chinese Holstein cows. Livestock Science 136, 164–168.
| Two novel SNPs of the type I gonadotropin releasing hormone receptor gene and their associations with superovulation traits in Chinese Holstein cows.Crossref | GoogleScholarGoogle Scholar |
Yang WC, Tang KQ, Zhang CY, Xu DQ, Wen QY, Yang LG (2011b) Polymorphism of the GnRHR gene and its association with litter size in Boer goats. South African Journal of Animal Science 41, 398–402.
| Polymorphism of the GnRHR gene and its association with litter size in Boer goats.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38Xlt1Wnsbk%3D&md5=3bc34b820d32b383fea5eb4ae4e4bfe3CAS |
