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RESEARCH ARTICLE

Effect of feed processing and enzyme supplementation on diet digestibility and performance of male weaner pigs fed wheat-based diets in dry or liquid form

Katie A. l’Anson A , Mingan Choct A and Peter H. Brooks B C
+ Author Affiliations
- Author Affiliations

A School of Environmental and Rural Science, University of New England, Armidale, NSW 2351, Australia.

B School of Biomedical and Biological Sciences, Plymouth University, Devon, PL4 8AA, England.

C Corresponding author. Email: pigprof@blueyonder.co.uk

Animal Production Science 53(6) 531-539 https://doi.org/10.1071/AN12256
Submitted: 27 July 2012  Accepted: 11 November 2012   Published: 2 April 2013

Abstract

One hundred and forty-four individually housed, entire male (Large White × Landrace) weaner pigs (28 days; 7.3 s.d. 0.1 kg) were individually housed and allocated to a 26-day feeding trial of 2 by 2 by 3 factorial design. The factors were (1) feed processing method (meal or steam pelleted), (2) feed form (dry or liquid), and (3) enzyme addition (basal diet, or basal diet plus 300 ppm xylanase or 100 ppm phytase). Five days post weaning, two pigs were removed from each treatment. The basal diet was formulated to be marginally adequate for pigs of this age, providing 13.8 MJ digestible energy (DE)/kg DM and 0.79 g available lysine/MJ DE. The diet contained 0.81% Ca, 0.44% non-phytate-P and 0.31% phytate-P. Compared with pigs fed dry diets, pigs fed liquid diets consumed less feed (70 ± 14.2 g/day; P < 0.05) had a lower daily gain (49 ± 12.5 g/day; P < 0.05) and as a consequence had a lower 26-day weight (1.1 ± 0.4 kg; P < 0.05). However, pigs fed liquid diets had a better feed conversion efficiency (adjusted to the same DM content as dry feed) than pigs on dry diets (1.16 versus 1.20 ± 0.02; P < 0.05). Steam pelleting the diets had no effect on feed intake or daily gain, but did improve feed conversion ratio (1.14 versus 1.22 ± 0.02; P < 0.05). The poorer growth on liquid feeds appeared to result from the feeding method imposing a degree of restriction on feed intake. Xylanase or phytase supplementation did not significantly affect non-starch polysaccharide levels in the diets or growth performance. Phytase supplementation increased (P < 0.05) daily intake, daily gain and 26-day weight, of pigs fed dry diets, but not those fed liquid diets. The results indicated that when diets were fed in liquid form, prior steam pelleting of the diets and enzyme supplementation did not provide additional benefit.

Additional keywords: feed intake, liquid feeding.


References

Ao X, Meng QW, Van L, Kim HJ, Hong SM, Cho JH, Kim IH (2010) Effects of non-starch polysaccharide-degrading enzymes on nutrient digestibility, growth performance and blood profiles of growing pigs fed a diet based on corn and soybean meal. Asian-Australasian Journal of Animal Sciences 23, 1632–1638.
Effects of non-starch polysaccharide-degrading enzymes on nutrient digestibility, growth performance and blood profiles of growing pigs fed a diet based on corn and soybean meal.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXhsVCiu78%3D&md5=95e4319f3f403e4a195a313ae36c9d50CAS |

Beal JD, Niven SJ, Brooks PH, Gill BP (2003) The effect of steeping on soluble phosphorus levels in wheat, wheatfeed and soyabean meal. In ‘Proceedings of the British Society of Animal Science 2003’. P. 64. (British Society of Animal Science: Penicuik, Scotland)

Bindelle J, Pieper R, Montoya CA, Van Kessel AG, Leterme P (2011) Nonstarch polysaccharide-degrading enzymes alter the microbial community and the fermentation patterns of barley cultivars and wheat products in an in vitro model of the porcine gastrointestinal tract. FEMS Microbiology Ecology 76, 553–563.
Nonstarch polysaccharide-degrading enzymes alter the microbial community and the fermentation patterns of barley cultivars and wheat products in an in vitro model of the porcine gastrointestinal tract.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXnt1Cgsrg%3D&md5=79f01fe204aa1db05a55d28edd70cfc5CAS |

Blaabjerg K, Carlson D, Hansen-Moller J, Tauson AH, Poulsen HD (2007) In vitro degradation of phytate and lower inositol phosphates in soaked diets and feedstuffs. Livestock Science 109, 240–243.
In vitro degradation of phytate and lower inositol phosphates in soaked diets and feedstuffs.Crossref | GoogleScholarGoogle Scholar |

Blaabjerg K, Carlsson NG, Hansen-Moller J, Poulsen HD (2010a) Effect of heat-treatment, phytase, xylanase and soaking time on inositol phosphate degradation in vitro in wheat, soybean meal and rapeseed cake. Animal Feed Science and Technology 162, 123–134.
Effect of heat-treatment, phytase, xylanase and soaking time on inositol phosphate degradation in vitro in wheat, soybean meal and rapeseed cake.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXhsVGrtLfF&md5=0991f7f17426a04f17092f955a675206CAS |

Blaabjerg K, Jorgensen H, Tauson AH, Poulsen HD (2010b) Heat-treatment, phytase and fermented liquid feeding affect the presence of inositol phosphates in ileal digesta and phosphorus digestibility in pigs fed a wheat and barley diet. Animal 4, 876–885.
Heat-treatment, phytase and fermented liquid feeding affect the presence of inositol phosphates in ileal digesta and phosphorus digestibility in pigs fed a wheat and barley diet.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXlsVOgt7c%3D&md5=b4bbe42e24fc370e0760ca099e52d2c7CAS |

Brooks PH (1999) The potential of liquid feeding systems. In ‘Concepts in pig science’. (Eds TP Lyons, DJA Cole) pp. 81–98. (Nottingham University Press: Nottingham)

Brooks PH (2008) Fermented liquid feed for pigs. CAB Reviews: Perspectives in Agriculture, Veterinary Science, Nutrition and Natural Resources 3, 18. Available at http://dx.doi.org/10.1079/PAVSNNR20083073 [Verified 13 October 2011]

Brooks PH, Beal JD, Niven SJ (2003) Liquid feeding of pigs 1: potential for reducing environmental impact and for improving productivity. Animal Science Papers and Reports 21, 7–22.

Canibe N, Jensen BB (2012) Fermented liquid feed – microbial and nutritional aspects and impact on enteric diseases in pigs. Animal Feed Science and Technology 173, 17–40.
Fermented liquid feed – microbial and nutritional aspects and impact on enteric diseases in pigs.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XjvVemu7c%3D&md5=f9a3126bbc24980d28f2d0da995cbcacCAS |

Chae BJ, Han IK (1998) Processing effects of feeds in swine – Review. Asian-Australasian Journal of Animal Sciences 11, 597–607.

Chae BJ, Han IK, Kim JH, Yang CJ, Ohh SJ, Rhee YC, Chung YK (1997) Effects of feed processing and feeding methods on growth and carcass traits for growing-finishing pigs. Asian-Australasian Journal of Animal Sciences 10, 164–169.

Chastanet F, Pahm AA, Pedersen C, Stein HH (2007) Effect of feeding schedule on apparent energy and amino acid digestibility by growing pigs. Animal Feed Science and Technology 132, 94–102.
Effect of feeding schedule on apparent energy and amino acid digestibility by growing pigs.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XhtF2qu7zJ&md5=8c3267bcc18b29688843082c09281e13CAS |

Choct M, Hughes RJ (1996) Long-chain hydrocarbons as a marker for digestibility studies in poultry. In ‘Proceedings of the Australian Poultry Science Symposium, 8’. (Ed. D Balnave) pp. 186–189 (Abstr.). (University of Sydney Press: Sydney)

Choct M, Hughes RJ (1997) The nutritive value of new season grains for poultry. In ‘Recent advances in animal nutrition in Australia’. (Eds J Corbett, M Choct, J Nolan, J Rowe) (University of New England: Armidale) Available at http://livestocklibrary.com.au/bitstream/handle/1234/19830/97_146.pdf?sequence=1 [Verified 21 August 2012]

Choct M, Dersjant-Li Y, McLeish J, Peisker M (2010) Soy oligosaccharides and soluble non-starch polysaccharides: a review of digestion, nutritive and anti-nutritive effects in pigs and poultry. Asian-Australasian Journal of Animal Sciences 23, 1386–1398.
Soy oligosaccharides and soluble non-starch polysaccharides: a review of digestion, nutritive and anti-nutritive effects in pigs and poultry.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXhsFemur%2FI&md5=85f0dbd8231536974c7233ebb6353049CAS |

Delgado-Andrade C, Rufian-Henares JA, Nieto R, Aguilera JF, Navarro MP, Seiquer I (2010) Does the pelleting process affect the nutritive value of a pre-starter diet for suckling piglets? Ex vivo studies on mineral absorption. Journal of the Science of Food and Agriculture 90, 898–905.
Does the pelleting process affect the nutritive value of a pre-starter diet for suckling piglets? Ex vivo studies on mineral absorption.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXivFWqtbo%3D&md5=0929c9feee7beb6cff091a1a0c91a0f6CAS |

García-Feliz C, Carvajal A, Collazos JA, Rubio P (2009) Herd-level risk factors for faecal shedding of Salmonella enterica in Spanish fattening pigs. Preventive Veterinary Medicine 91, 130–136.
Herd-level risk factors for faecal shedding of Salmonella enterica in Spanish fattening pigs.Crossref | GoogleScholarGoogle Scholar |

Han YK, Thacker PA, Yang JS (2006) Effects of the duration of liquid feeding on performance and nutrient digestibility in weaned pigs. Asian-Australasian Journal of Animal Sciences 19, 396–401.

Hansen CF (2004) Choice of dry feed influences gastric conditions, incidence of Salmonella and performance in growing-finishing pigs. PhD thesis, The Royal Veterinary and Agricultural University, Copenhagen.

Högberg A, Lindberg JE (2006) The effect of level and type of cereal non-starch polysaccharides on the performance, nutrient utilization and gut environment of pigs around weaning. Animal Feed Science and Technology 127, 200–219.
The effect of level and type of cereal non-starch polysaccharides on the performance, nutrient utilization and gut environment of pigs around weaning.Crossref | GoogleScholarGoogle Scholar |

Jensen BB, Mikkelsen LL (1998) Feeding liquid diets to pigs. In ‘Recent advances in animal nutrition 1998’. (Eds PC Garnsworthy, J Wiseman) pp. 107–126. (Nottingham University Press: Thrumpton, Nottingham)

Jha R, Overend DN, Simmins PN, Hickling D, Zijlstra RT (2011) Chemical characteristics, feed processing quality, growth performance and energy digestibility among wheat classes in pelleted diets fed to weaned pigs. Animal Feed Science and Technology 170, 78–90.
Chemical characteristics, feed processing quality, growth performance and energy digestibility among wheat classes in pelleted diets fed to weaned pigs.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXhtlyktr7M&md5=fc3132fa4f883b222b13a7e2adb3974eCAS |

Kiarie E, Owusu-Asiedu A, Simmins PH, Nyachoti CM (2010) Influence of phytase and carbohydrase enzymes on apparent ileal nutrient and standardized ileal amino acid digestibility in growing pigs fed wheat and barley-based diets. Livestock Science 134, 85–87.
Influence of phytase and carbohydrase enzymes on apparent ileal nutrient and standardized ileal amino acid digestibility in growing pigs fed wheat and barley-based diets.Crossref | GoogleScholarGoogle Scholar |

l’Anson KA, Choct M, Brooks PH (2012) The influence of particle size and processing method for wheat-based diets, offered in dry or liquid form, on growth performance and diet digestibility in male weaner pigs. Animal Production Science 52, 899–904.
The influence of particle size and processing method for wheat-based diets, offered in dry or liquid form, on growth performance and diet digestibility in male weaner pigs.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38Xht1Grs7fL&md5=8da2bc9f04151c59143783fa6d800462CAS |

Lahaye L, Riou Y, Seve B (2007) The effect of grinding and pelleting of wheat and maize on amino acids true ileal digestibility and endogenous losses in growing pigs. Livestock Science 109, 138–140.
The effect of grinding and pelleting of wheat and maize on amino acids true ileal digestibility and endogenous losses in growing pigs.Crossref | GoogleScholarGoogle Scholar |

Lahaye L, Ganier P, Thibault JN, Riou Y, Seve B (2008) Impact of wheat grinding and pelleting in a wheat-rapeseed meal diet on amino acid ileal digestibility and endogenous losses in pigs. Animal Feed Science and Technology 141, 287–305.
Impact of wheat grinding and pelleting in a wheat-rapeseed meal diet on amino acid ileal digestibility and endogenous losses in pigs.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXislWhtbY%3D&md5=3022532c9e1c0da2b6fc8a03214b811bCAS |

Lehnen CR, Lovatto PA, Andretta I, Kipper M, Hauschild L, Rossi CA (2011) Meta-analysis of ileal digestibility of amino acids and minerals in pigs fed diets containing enzymes. Pesquisa Agropecuaria Brasileira 46, 438–445.
Meta-analysis of ileal digestibility of amino acids and minerals in pigs fed diets containing enzymes.Crossref | GoogleScholarGoogle Scholar |

Lei XG, Stahl CH (2000) Nutritional benefits of phytase and dietary determinants of its efficacy. Journal of Applied Animal Research 17, 97–112.
Nutritional benefits of phytase and dietary determinants of its efficacy.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXivF2msr8%3D&md5=61be497b8290e7f065b85f41bb1f804aCAS |

Lundblad KK, Issa S, Hancock JD, Behnke KC, McKinney LJ, Alavi S, Prestlokken E, Fledderus J, Sorensen M (2011) Effects of steam conditioning at low and high temperature, expander conditioning and extruder processing prior to pelleting on growth performance and nutrient digestibility in nursery pigs and broiler chickens. Animal Feed Science and Technology 169, 208–217.
Effects of steam conditioning at low and high temperature, expander conditioning and extruder processing prior to pelleting on growth performance and nutrient digestibility in nursery pigs and broiler chickens.Crossref | GoogleScholarGoogle Scholar |

Lundblad KK, Hancock JD, Behnke KC, McKinney LJ, Alavi S, Prestlokken E, Sorensen M (2012) Ileal digestibility of crude protein, amino acids, dry matter and phosphorous in pigs fed diets steam conditioned at low and high temperature, expander conditioned or extruder processed. Animal Feed Science and Technology 172, 237–241.
Ileal digestibility of crude protein, amino acids, dry matter and phosphorous in pigs fed diets steam conditioned at low and high temperature, expander conditioned or extruder processed.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XivVyiuro%3D&md5=0bcf6ddd7662085792aabc446fd72cb7CAS |

Lyberg K (2006) Phosphorus in pig diets. Effect of liquid feeding, phosphorus levels and phytase supplementation on digestibility and performance. PhD thesis, Swedish University of Agricultural Sciences, Uppsala.

Lyberg K, Simonsson A, Lindberg JE (2005) Influence of phosphorus level and soaking of food on phosphorus availability and performance in growing-finishing pigs. Animal Science 81, 375–381.
Influence of phosphorus level and soaking of food on phosphorus availability and performance in growing-finishing pigs.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XmvFegtg%3D%3D&md5=ce1d8c49c3d6078f0032900bb9fe5227CAS |

Lyberg K, Lundh T, Pedersen C, Lindberg JE (2006) Influence of soaking, fermentation and phytase supplementation on nutrient digestibility in pigs offered a grower diet based on wheat and barley. Animal Science 82, 853–858.
Influence of soaking, fermentation and phytase supplementation on nutrient digestibility in pigs offered a grower diet based on wheat and barley.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXis1Sjt7c%3D&md5=385251b144496fbc1d248e753d61bbb9CAS |

McCann MEE, McEvoy JDG, McCracken KJ, Simmins PH (2006) Effect of variety, endosperm hardness, the 1B/1R translocation and enzyme addition on the nutritive value of wheat for growing pigs. Irish Journal of Agricultural and Food Research 45, 173–185.

Missotten JAM, Michiels J, Ovyn A, De Smet S, Dierick NA (2010) Fermented liquid feed for pigs. Archives of Animal Nutrition 64, 437–466.
Fermented liquid feed for pigs.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXhtlyktbrK&md5=01bb86d4c5534073443074b8be4c6b4aCAS |

Mosenthin R, Broz J (2010) Mineral digestibility and environmental issues. Efficacy and interactions of phytases. Livestock Science 134, 258–260.
Mineral digestibility and environmental issues. Efficacy and interactions of phytases.Crossref | GoogleScholarGoogle Scholar |

Nitrayová S, Heger J, Patras P, Kluge H, Broz J (2009) Effect of xylanase on apparent ileal and total tract digestibility of nutrients and energy of rye in young pigs. Archives of Animal Nutrition 63, 281–291.
Effect of xylanase on apparent ileal and total tract digestibility of nutrients and energy of rye in young pigs.Crossref | GoogleScholarGoogle Scholar |

Nortey TN, Patience JF, Sands JS, Trottier NL, Zijlstra RT (2008) Effects of xylanase supplementation on the apparent digestibility and digestible content of energy, amino acids, phosphorus, and calcium in wheat and wheat by-products from dry milling fed to grower pigs. Journal of Animal Science 86, 3450–3464.
Effects of xylanase supplementation on the apparent digestibility and digestible content of energy, amino acids, phosphorus, and calcium in wheat and wheat by-products from dry milling fed to grower pigs.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXhsV2jsb3J&md5=3e18687a25cd8e397db80d1ba581a787CAS |

O’Connor AM, Denagamage T, Sargeant JM, Rajic A, McKean J (2008) Feeding management practices and feed characteristics associated with Salmonella prevalence in live and slaughtered market-weight finisher swine: a systematic review and summation of evidence from 1950 to 2005. Preventive Veterinary Medicine 87, 213–228.
Feeding management practices and feed characteristics associated with Salmonella prevalence in live and slaughtered market-weight finisher swine: a systematic review and summation of evidence from 1950 to 2005.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD1cnmtFWgtg%3D%3D&md5=55cdb9026a2f756f90a471a9a3da9151CAS |

Owusu-Asiedu A, Simmins PH, Brufau J, Lizardo R, Peron A (2010) Effect of xylanase and beta-glucanase on growth performance and nutrient digestibility in piglets fed wheat-barley-based diets. Livestock Science 134, 76–78.
Effect of xylanase and beta-glucanase on growth performance and nutrient digestibility in piglets fed wheat-barley-based diets.Crossref | GoogleScholarGoogle Scholar |

Pallauf J, Rimbach G (1997) Nutritional significance of phytic acid and phytase. Archives of Animal Nutrition 50, 301–319.

Pedersen C, Stein HH (2010) Effects of liquid and fermented liquid feeding on energy, dry matter, protein and phosphorus digestibility by growing pigs. Livestock Science 134, 59–61.
Effects of liquid and fermented liquid feeding on energy, dry matter, protein and phosphorus digestibility by growing pigs.Crossref | GoogleScholarGoogle Scholar |

Peo ER, Libal GW, Wehrbein GF, Cunningham PJ, Vipperman PE (1969) Effect of dietary increments of calcium and phosphorus on growing-fattening swine. Journal of Animal Science 29, 141

Plumed-Ferrer C, von Wright A (2009) Fermented pig liquid feed: nutritional, safety and regulatory aspects. Journal of Applied Microbiology 106, 351–368.
Fermented pig liquid feed: nutritional, safety and regulatory aspects.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXjtFKltbo%3D&md5=c039f00ca56313f7c485319ed7e0256cCAS |

Poulsen HD, Blaabjerg K, Feuerstein D (2007) Comparison of different levels and sources of microbial phytases. Livestock Science 109, 255–257.
Comparison of different levels and sources of microbial phytases.Crossref | GoogleScholarGoogle Scholar |

Poulsen HD, Carlson D, Norgaard JV, Blaabjerg K (2010) Phosphorus digestibility is highly influenced by phytase but slightly by calcium in growing pigs. Livestock Science 134, 100–102.
Phosphorus digestibility is highly influenced by phytase but slightly by calcium in growing pigs.Crossref | GoogleScholarGoogle Scholar |

Russell PJ, Geary TM, Brooks PH, Campbell A (1996) Performance, water use and effluent output of weaner pigs fed ad libitum with either dry pellets or liquid feed and the role of microbial activity in the liquid feed. Journal of the Science of Food and Agriculture 72, 8–16.
Performance, water use and effluent output of weaner pigs fed ad libitum with either dry pellets or liquid feed and the role of microbial activity in the liquid feed.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK28XlslOku78%3D&md5=bf7c07babc803c2e1f7703e03eab6398CAS |

Stege H, Jensen TK, Moller K, Baekbo P, Jorsal SE (2001) Risk factors for intestinal pathogens in Danish finishing pig herds. Preventive Veterinary Medicine 50, 153–164.
Risk factors for intestinal pathogens in Danish finishing pig herds.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD3Mzpslyksg%3D%3D&md5=87b0a8689ee4f4d00fb72a47c2757de5CAS |

Svihus B, Zimonja O (2011) Chemical alterations with nutritional consequences due to pelleting animal feeds: a review. Animal Production Science 51, 590–596.
Chemical alterations with nutritional consequences due to pelleting animal feeds: a review.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXnvF2hsb8%3D&md5=ef3a1cdb2af7fb985bd29f572b61709bCAS |

Theander O, Andersson R, Åman P, Westerlund E (2001) Determination of total dietary fiber and its individual components by the Uppsala method. In ‘CRC handbook of dietary fiber in human nutrition’. 3rd edn. (Ed. GA Spiller) p. 265. (CRC Press: Boca Raton, FL) Available at http://www.crcnetbase.com/doi/abs/10.1201/9781420038514.ch3.5 [Verified 20 December 2012]

Thorpe J, Miller BG, Schulze H (1998) The effects of liquid feeding at different feed intervals on ileal digestibility in the early weaned pig. In ‘Book of Abstracts of the 49th Meeting of the Association of the European Association of Animal Production, Warsaw 24–29 August’. (Eds JAM van Arendonk, V Ducrocq, Y van der Honing, F Madec, T van der Lende, D Puller, J Folch, EW Fernandez and EW Bruns) (Wageningen Pers: Wageningen)

White GA, Doucet FJ, Hill SE, Wiseman J (2008) Physicochemical properties and nutritional quality of raw cereals for newly weaned piglets. Animal 2, 867–878.
Physicochemical properties and nutritional quality of raw cereals for newly weaned piglets.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXhvVKgur0%3D&md5=bb8b7f86213a3080f8100c5692fc89e0CAS |

Wilkins W, Rajic A, Waldner C, McFall M, Chow E, Muckle A, Rosengren L (2010) Distribution of Salmonella serovars in breeding, nursery, and grow-to-finish pigs, and risk factors for shedding in ten farrow-to-finish swine farms in Alberta and Saskatchewan. Canadian Journal of Veterinary Research-Revue Canadienne De Recherche Veterinaire 74, 81–90.

Zijlstra RT, de Lange CFM, Patience JF (1999) Nutritional value of wheat for growing pigs: chemical composition and digestible energy content. Canadian Journal of Animal Science 79, 187–194.
Nutritional value of wheat for growing pigs: chemical composition and digestible energy content.Crossref | GoogleScholarGoogle Scholar |

Zijlstra RT, Owusu-Asiedu A, Simmins PH (2010) Future of NSP-degrading enzymes to improve nutrient utilization of co-products and gut health in pigs. Livestock Science 134, 255–257.
Future of NSP-degrading enzymes to improve nutrient utilization of co-products and gut health in pigs.Crossref | GoogleScholarGoogle Scholar |