Prevalence of mimosine and DHP toxicity in cattle grazing Leucaena leucocephala pastures in Queensland, Australia
S. A. Dalzell A , D. J. Burnett A , J. E. Dowsett A , V. E. Forbes A and H. M. Shelton A B
+ Author Affiliations
- Author Affiliations
A School of Agriculture and Food Sciences, The University of Queensland, St Lucia, Qld 4072, Australia.
B Corresponding author. Email: m.shelton@uq.edu.au
Animal Production Science 52(5) 365-372 https://doi.org/10.1071/AN11236
Submitted: 17 October 2011 Accepted: 9 February 2012 Published: 10 May 2012
Abstract
A postal survey of the level of awareness of leucaena toxicity and an on-farm study of the toxicity status of Queensland cattle herds grazing leucaena were conducted to investigate the prevalence of mimosine and dihydroxypyridine (DHP) toxicity in Queensland. In total, 195 of 356 graziers surveyed responded to the postal survey. Sixty-three percent had inoculated their cattle with in vitro Synergistes jonesii inoculum (produced in an anaerobic fermenter) and 30% of these had inoculated more than once. The remainder used inappropriate procedures. Many graziers (43%) had occasionally observed toxicity symptoms of hair loss and poor animal growth rates. In the on-farm study, the toxicity status of 385 animals in 44 individually managed herds on 36 properties was determined by urine analysis of mimosine and DHP concentrations. No animals were experiencing mimosine toxicity, based on low concentrations of this compound found in the urine. Using the criterion that average herd urine concentrations of DHP >100 μg/mL was indicative of subclinical toxicity, 48% of herds were exposed to subclinical toxicity due to dominant 3,4-DHP (21%) or dominant 2,3-DHP (27%) toxicity; many of these herds had been inoculated with S. jonesii and were thought to be protected. The finding that 27% of herds were excreting high concentrations of 2,3-DHP was unexpected. Statistical analysis of herd-management data revealed that the method used by graziers to inoculate their herds was significantly (P < 0.05) but weakly linked to herd protection status. It was concluded that subclinical 3,4-DHP and 2,3-DHP toxicity remains a problem in Queensland and is likely to be limiting animal production in a significant number of cattle grazing leucaena-grass pastures.
Additional keywords: 3-hydroxy-4(1H)-pyridine (3,4-DHP), 2-hydroxy-3(1H)-pyridine (2,3-DHP).
References
Allison MJ, Mayberry WR, McSweeney CS, Stahl DA (1992) Synergistes jonesii, gen. nov., sp. nov.: a rumen bacterium that degrades toxic pyridinediols. Systematic and Applied Microbiology 15, 522–529.| Synergistes jonesii, gen. nov., sp. nov.: a rumen bacterium that degrades toxic pyridinediols.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3sXisFWhsrY%3D&md5=740e98fc4a80d204295ac2e262b30033CAS |
Bolam MJ (1998) Manipulation of the supply of protein and energy to ruminants consuming tropical forage through supplementation strategies. PhD Thesis, The University of Queensland, Brisbane.
Domínguez-Bello MG, Lovera M, Rincón MT (1997) Characteristics of dihydroxypyridine-degrading activity in the rumen bacterium Synergistes jonesii. FEMS Microbiology Ecology 23, 361–365.
| Characteristics of dihydroxypyridine-degrading activity in the rumen bacterium Synergistes jonesii.Crossref | GoogleScholarGoogle Scholar |
Ford CW, Megarrity RG, Meehan GV (1984) 2,3-DHP, a novel mimosine metabolite. Leucaena Research Reports 5, 2
Galgal KK (2002) Forage and animal production from selected new Leucaena accessions. PhD Thesis, The University of Queensland, Brisbane.
Ghosh MK, Atreja PP, Buragohain R, Bandyopadhyay S (2007) Influence of short-term Leucaena leucocephala feeding on milk yield and its composition, thyroid hormones, enzyme activity, and secretion of mimosine and its metabolites in milk of cattle. The Journal of Agricultural Science 145, 407–414.
| Influence of short-term Leucaena leucocephala feeding on milk yield and its composition, thyroid hormones, enzyme activity, and secretion of mimosine and its metabolites in milk of cattle.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXptVSgu7o%3D&md5=4a8bd12df8ea404cca87a8d9d50a965fCAS |
Guyton AC, Hall JE (2000) ‘Textbook of medical physiology.’ 10th edn. (W.B. Saunders Company: Philadelphia, PA)
Hammond AC, Allison MJ, Williams MJ, Prine GM, Bates DB (1989) Prevention of leucaena toxicosis of cattle in Florida by ruminal inoculation with 3-hydroxy-4-(1H)-pyridone-degrading bacteria. American Journal of Veterinary Research 50, 2176–2180.
Hart NK, Hofmann A, Lamberton JA, Richards CM (1977) Mimosine, mimosinamine and 3,4-dihydroxypyridine. Heterocycles 7, 265–272.
| Mimosine, mimosinamine and 3,4-dihydroxypyridine.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaE1cXht1Sqtr0%3D&md5=fa3b296ec1a143fd2ca0350dd4324cebCAS |
Hegarty MP, Schinckel PG, Court RD (1964) Reaction of sheep to consumption of Leucaena glauca Benth. and to its toxic principle mimosine. Australian Journal of Agricultural Research 15, 153–167.
| Reaction of sheep to consumption of Leucaena glauca Benth. and to its toxic principle mimosine.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaF2cXptlOhtw%3D%3D&md5=09fa2aada7ac50887bf3f7dcac2f8107CAS |
Hegarty MP, Court RD, Christie GS, Lee CP (1976) Mimosine in Leucaena leucocephala is metabolized to a goitrogen in ruminants. Australian Veterinary Journal 52, 490
| Mimosine in Leucaena leucocephala is metabolized to a goitrogen in ruminants.Crossref | GoogleScholarGoogle Scholar |
Jones RJ (1994) Management of anti-nutritive factors – with special reference to leucaena. In ‘Forage tree legumes in tropical agriculture’. (Eds RC Gutteridge, HM Shelton) pp. 216–231. (CABI: Wallingford, UK)
Jones RJ, Hegarty MP (1984) The effect of different proportions of Leucaena leucocephala in the diet of cattle on growth, feed-intake, thyroid-function and urinary-excretion of 3-hydroxy-4(1h)-pyridone. Australian Journal of Agricultural Research 35, 317–325.
| The effect of different proportions of Leucaena leucocephala in the diet of cattle on growth, feed-intake, thyroid-function and urinary-excretion of 3-hydroxy-4(1h)-pyridone.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL2cXkvVGgsrk%3D&md5=fe136725fbc6d45ec65adf1482a00fe6CAS |
Jones RM, Jones RJ (1984) The effect of Leucaena leucocephala on liveweight gain, thyroid size and thyroxine levels of steers in south-eastern Queensland. Australian Journal of Experimental Agriculture and Animal Husbandry 24, 4–9.
| The effect of Leucaena leucocephala on liveweight gain, thyroid size and thyroxine levels of steers in south-eastern Queensland.Crossref | GoogleScholarGoogle Scholar |
Jones RJ, Megarrity RG (1986) Successful transfer of DHP-degrading bacteria from Hawaiian goats to Australian ruminants to overcome the toxicity of leucaena. Australian Veterinary Journal 63, 259–262.
| Successful transfer of DHP-degrading bacteria from Hawaiian goats to Australian ruminants to overcome the toxicity of leucaena.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaL2s%2FnsFCqsQ%3D%3D&md5=81fc5f003f476063789adf5a344b27ecCAS |
Jones RJ, Winter WH (1982) Serum thyroxine levels and liveweight gain of steers grazing leucaena pastures. Leucaena Research Reports 3, 2
Jones RJ, Ludlow MM, Troughton JH, Blunt CG (1979) Estimation of the proportion of C3 and C4 plant species in the diet of animals from the ratio of natural 12C and 13C isotopes in the faeces. Journal of Agricultural Science, Cambridge 92, 91–100.
| Estimation of the proportion of C3 and C4 plant species in the diet of animals from the ratio of natural 12C and 13C isotopes in the faeces.Crossref | GoogleScholarGoogle Scholar |
Jones RJ, Ford CW, Megarrity RG (1985a) Conversion of 3,4-DHP to 2,3-DHP by rumen bacteria. Leucaena Research Reports 6, 3–4.
Jones RJ, Lowry JB, Megarrity RG (1985b) Transfer of DHP-degrading bacteria from adapted to unadapted ruminants. Leucaena Research Reports 6, 5–6.
Jones RJ, Coates DB, Palmer B (2009) Survival of the rumen bacterium Synergistes jonesii in a herd of Droughtmaster cattle in north Queensland. Animal Production Science 49, 643–645.
| Survival of the rumen bacterium Synergistes jonesii in a herd of Droughtmaster cattle in north Queensland.Crossref | GoogleScholarGoogle Scholar |
Klieve AV, Ouwerkerk D, Turner A, Roberton R (2002) The production and storage of a fermentor-grown bacterial culture containing Synergistes jonesii, for protecting cattle against mimosine and 3-hydroxy-4(1H)-pyridone toxicity from feeding on Leucaena leucocephala. Australian Journal of Agricultural Research 53, 1–5.
| The production and storage of a fermentor-grown bacterial culture containing Synergistes jonesii, for protecting cattle against mimosine and 3-hydroxy-4(1H)-pyridone toxicity from feeding on Leucaena leucocephala.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XhsVGrtL4%3D&md5=6a4a3819a64fddcf8bd129b874adfed7CAS |
Lee CP, Christie GS, Hegarty MP (1980) Anti-thyroid and anti-perioxidase activity of some isomeric dihydroxy pyrodines. Thyroid Research III. In ‘Proceedings of the 8th international thyroid congress’, 1980, Sydney, p. 137.
Lowry JB, Tangendjaja M, Tangendjaja B (1983) Autolysis of mimosine to 3-hydroxy-4–1(H)pyridine in green tissues of Leucaena leucocephala. Journal of the Science of Food and Agriculture 34, 529–533.
| Autolysis of mimosine to 3-hydroxy-4–1(H)pyridine in green tissues of Leucaena leucocephala.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL3sXks12iur8%3D&md5=e61c7cfa46923b1030808000301ef93aCAS |
McSweeney CS, Bamualim A, Murray RM (1984) Ruminal motility in sheep intoxicated with 2,3-dihydroxypyridine. Australian Veterinary Journal 61, 271–272.
| Ruminal motility in sheep intoxicated with 2,3-dihydroxypyridine.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaL2M%2FntF2gsA%3D%3D&md5=8ff6fc05bed7229679266ec1eb565a23CAS |
Phaikaew C, Suksaran W, Ted-arsen J, Nakamanee G, Saichuer A. Seejundee S, Kotprom N, Shelton HM (2012) Incidence of subclinical toxicity in goats and dairy cows consuming leucaena (Leucaena leucocephala) in Thailand. Animal Production Science 52, 283–286.
| Incidence of subclinical toxicity in goats and dairy cows consuming leucaena (Leucaena leucocephala) in Thailand.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XktFWgs7c%3D&md5=6efcac388c1e6a56070a1f94dac3a1ebCAS |
Prasad J, Paliwal OP (1989) Pathological changes in experimentally induced leucaena toxicity in lambs. The Indian Veterinary Journal 66, 711–714.
Pratchett D, Jones RJ, Syrch FX (1991) Use of DHP-degrading rumen bacteria to overcome toxicity in cattle grazing irrigated leucaena pasture. Tropical Grasslands 25, 268–274.
Puchala R, Sahlu T, Davis JJ, Hart SP (1995) Influence of mineral supplementation on 2,3-dihydroxypyridine toxicity in Angora goats. Animal Feed Science and Technology 55, 253–262.
| Influence of mineral supplementation on 2,3-dihydroxypyridine toxicity in Angora goats.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2MXpvFyku70%3D&md5=d13fac65edf2541b5e77db5961e61205CAS |
Quirk MF, Bushell JJ, Jones RJ, Megarrity RG, Butler KL (1988) Live-weight gains on leucaena and native grass pastures after dosing cattle with rumen bacteria capable of degrading DHP, a ruminal metabolite from leucaena. Journal of Agricultural Science 111, 165–170.
| Live-weight gains on leucaena and native grass pastures after dosing cattle with rumen bacteria capable of degrading DHP, a ruminal metabolite from leucaena.Crossref | GoogleScholarGoogle Scholar |
Raurela M, Jones RJ (1985) Degradation of DHP in cattle in Papua New Guinea. Leucaena Research Reports 6, 68
Rincón MT, Allison MJ, Michelangeli F, De Sanctis Y, Domínguez-Bello MG (1998) Anaerobic degradation of mimosine-derived hydroxypyridines by cell free extracts of the rumen bacterium Synergistes jonesii. FEMS Microbiology Ecology 27, 127–132.
| Anaerobic degradation of mimosine-derived hydroxypyridines by cell free extracts of the rumen bacterium Synergistes jonesii.Crossref | GoogleScholarGoogle Scholar |
Rincón MT, Domínguez-Bello MG, Lovera M, Romero R (2000) Degradation of toxic piridinediols derived from mimosine by rumen bacteria: I. Microbiological aspects. Revista Cientifica-Facultad De Ciencias Veterinarias 10, 222–232.
Shelton M, Dalzell S (2007) Production, economic and environmental benefits of leucaena pastures. Tropical Grasslands 41, 174–190.
Tangendjaja B, Wills RBH (1980) Analysis of mimosine and 3-hydroxy-4(1H)-pyridone by high- performance liquid chromatography. Journal of Chromatography. A 202, 317–318.
| Analysis of mimosine and 3-hydroxy-4(1H)-pyridone by high- performance liquid chromatography.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL3MXhsFaktb8%3D&md5=bdf731eae8224e295e788add709568d5CAS |
