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RESEARCH ARTICLE
Contents Vol 50(6)

Eastern grey kangaroo (Macropus giganteus) myofibres. 2. Characteristics of eight skeletal muscles

N. B. Spiegel A B E , P. C. Wynn C , J. M. Thompson A and P. L. Greenwood D F
+ Author Affiliations
- Author Affiliations

A School of Environmental and Rural Science, University of New England, Armidale, NSW 2351, Australia.

B Faculty of Veterinary Science, University of Sydney, Sydney, NSW 2006, Australia.

C E. H. Graham Centre for Agricultural Innovation, Charles Sturt University, Wagga Wagga, NSW 2678, Australia.

D Industry & Investment NSW, University of New England, Armidale, NSW 2351, Australia.

E Present address: School of Environmental Science, Murdoch University, Murdoch, WA 6150, Australia.

F Corresponding author. Email: paul.greenwood@industry.nsw.gov.au

Animal Production Science 50(6) 393-399 https://doi.org/10.1071/AN09196
Submitted: 11 December 2009  Accepted: 14 April 2010   Published: 11 June 2010

Abstract

The myofibre characteristics of eight skeletal muscles of economic importance, comprising six muscles from the upper hindlimb, one from the lumbar and one from the sacral region, from five eastern grey kangaroos (Macropus giganteus) were determined. Differential staining of myosin heavy chains allowed myofibres to be classified as Types 1 (slow oxidative), 2A (fast oxidative-glycolytic) and 2X/2B (fast glycolytic), as well as the intermediate or transitional Types 2C (Type 1–Type 2A intermediate) and 2AX/B (Type 2A–Type 2X/2B intermediate). The m. psoas minor had a higher area comprising Type 1 myofibres (41.4%) relative to total myofibre area than did any of the other muscles studied (each <5%). This was due to the m. psoas minor having a higher percentage (31.9%) and larger average cross-sectional area (CSA; 4211 µm2) of Type 1 myofibres. Type 2X/2B myofibres comprised over 70% of the relative area in the mm. semimembranosus, semitendinosus and gluteus medius, compared with 34.2% in the m. psoas minor, with the other muscles intermediate. The proportion of Type 2A myofibres ranged from 19.1% (m. gluteus medius) to 34.6% (m. caudal dorsolateral sacrocaudalis) of the relative myofibre area. The m. caudal dorsolateral sacrocaudalis had the largest average myofibre CSA and the m. adductor the smallest (5539 and 2455 µm2, respectively). Among the intermediate myofibre types, Type 2AX/B myofibres were more prevalent (range 4.3%–13.0% of myofibres) than Type 2C myofibres (≤0.5%). Overall, the correlations between carcass weight and the percentage and relative areas of myofibres were positive for Type 2A and negative for Type 2X/2B myofibres. The results provide a detailed characterisation of myofibres in kangaroo skeletal muscles of economic importance. Furthermore, they enhance our understanding of factors influencing kangaroo muscle structure and post-mortem metabolism and provide potential indicators of eating quality of kangaroo meat.

Additional keyword: macropod.


Acknowledgements

Financial support was provided by the Rural Industries Research and Development Corporation (RIRDC) and the Kangaroo Industry Association of Australia (KIAA). The assistance provided by technical staff of the NSW Department of Primary Industries Beef Industry Centre and Dr Ian Colditz (CSIRO, Armidale, NSW, Australia) is also gratefully acknowledged. Constructive editorial comments provided by Dr Paul Hopwood during the preparation of this manuscript are greatly appreciated. The authors also acknowledge Dr Brigitte Picard (INRA, Institut National de la Recherche Agronomique) who provided antibody S5 8H2.


References


Armstrong RB (1980) Properties and distributions of the fibre types in the locomotory muscles of mammals. In ‘Comparative physiology: Primitive mammals’. (Eds K Schmidt-Nielsen, L Bolis, CR Taylor) pp. 243–254. (Cambridge University Press: Cambridge)

Bennett MB (1999) Foot areas, ground reaction forces and pressures beneath the feet of kangaroos, wallabies and rat-kangaroos (Marsupialia: Macropodoidea). Journal of Zoology 247, 365–369.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Briand M, Talmant A, Briand Y, Monin G, Durand R (1981) Metabolic types of muscle in sheep: I. Myosin ATPase, glycolytic and mitochondrial enzyme activities. European Journal of Applied Physiology and Occupational Physiology 46, 347–358.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Caughley G , Shepherd N , Short J (1987) ‘Kangaroos, their ecology and management in the sheep rangelands of Australia.’ (Cambridge University Press: Cambridge)

Dubowitz V , Brooke MH (1973) Normal muscle. In ‘Muscle biopsy: A modern approach’. pp. 34–73. (WB Saunders: London)

Gardner GE, Pethick DW, Greenwood PL, Hegarty RS (2006) The effect of genotype and plane of nutrition on rate of pH decline post mortem and the expression of enzymatic markers of metabolism in lamb carcases. Australian Journal of Agricultural Research 57, 661–670.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Gardner GE, Hopkins DL, Greenwood PL, Cake MA, Boyce MD, Pethick DW (2007) Sheep genotype, age and muscle type affect the expression of metabolic enzyme markers. Australian Journal of Experimental Agriculture 47, 1180–1189.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Genstat (2007) ‘Genstat edition 10.1.’ (Lawes Agricultural Trust: Rothamsted)

Greenwood PL, Davis J, Gaunt GM, Ferrier GR (2006a) Influences on the loin and cellular characteristics of the m. longissimus lumborum of Australian Poll Dorset-sired lambs. Australian Journal of Agricultural Research 57, 1–12.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Greenwood PL, Gardner GE, Hegarty RS (2006b) Lamb myofibre characteristics are influenced by sire estimated breeding values and pastoral nutritional system. Australian Journal of Agricultural Research 57, 627–639.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Greenwood PL, Harden S, Hopkins DL (2007) Myofibre characteristics of ovine longissimus and semitendinosus muscles are influenced by sire breed, gender, rearing type, age, and carcass weight. Australian Journal of Experimental Agriculture 47, 1137–1146.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Greenwood PL, Tomkins NW, Hunter RA, Allingham PG, Harden S, Harper GS (2009) Bovine myofiber characteristics are influenced by postweaning nutrition. Journal of Animal Science 87, 3114–3123.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Grigg G (1988) Kangaroo harvesting and the conservation of the sheep rangelands. Australian Zoologist 24, 124–128. open url image1

Grigg G (2002) The impact of animals on the environment: Should we be switching to kangaroos and, if so, how could we? A paper to stimulate discussion. Animal Production in Australia 24, 425–434. open url image1

Hopwood PR, Butterfield RM (1976) The musculature of the proximal pelvic limb of the eastern grey kangaroo Macropus major (Shaw) Macropus giganteus (Zimm). Journal of Anatomy 121, 259–277.
|  CAS | PubMed |
open url image1

Hopwood PR, Hilmi M, Butterfield RM (1976) A comparative study of the carcass composition of kangaroos and sheep. Australian Journal of Zoology 24, 1–6.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Klieve AV , Ouwerkerk D (2007) Comparative greenhouse gas emissions from herbivores. In ‘Proceedings of the 7th International Symposium on the Nutrition of Herbivores’. (Eds QX Meng, LP Ren, ZJ Cao) pp. 487–500. (China Agricultural University Press: Beijing)

O’Dea K (1988) Kangaroo meat – polyunsaturated and low in fat: Ideal for cholesterol-lowering diets. Australian Zoologist 24, 140–143. open url image1

Park SK, Gunawan AM, Scheffler TL, Grant AL, Gerrard DE (2009) Myosin heavy chain isoform content and energy metabolism can be uncoupled in pig skeletal muscle. Journal of Animal Science 87, 522–531.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Picard B, Duris MP, Jurie C (1998) Classification of bovine muscle fibres by different histochemical techniques. The Histochemical Journal 30, 473–477.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Rehfeldt C, Fiedler I, Dietl G, Ender K (2000) Myogenesis and postnatal muscle cell growth as influenced by selection. Livestock Production Science 66, 177–188.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Sayd T, Mera T, Martin VL, Laville E (1998) Spatial distribution of myosin heavy chain isoforms and lactate dehydrogenase M4 in the limb musculature of two crossbred lambs. Comparative Biochemistry and Physiology Part B 120, 153–163.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Spiegel NB (2008) Factors influencing the quality of meat from kangaroos. PhD Thesis, The University of Sydney, Australia.

Spiegel NB, Beaton AJW, McGrath J, Thompson JM, Wynn PC, Greenwood PL (2002) Myofibre types in eight skeletal muscles from the eastern grey kangaroo (Macropus giganteus). Animal Production in Australia 24, 225–228. open url image1

Spiegel NB, Johns WH, Sinclair SD, Wynn PC, Thompson JM, Greenwood PL (2010) Eastern grey kangaroo (Macropus giganteus) myofibres. 1. A simplified classification method using two commercially available antibodies. Animal Production Science 50, 386–392.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Suzuki A, Cassens RG (1983) A histochemical study of myofibre types in the serratus ventralis thoracis muscle of sheep during growth. Journal of Animal Science 56, 1447–1458.
|  CAS | PubMed |
open url image1

Totland GK, Kryvi H (1991) Distribution patterns of muscle-fiber types in major muscles of the bull (Bos taurus). Anatomy and Embryology 184, 441–450.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Warner RD, Pethick DW, Greenwood PL, Ponnampalam EN, Banks RG, Hopkins DL (2007) Unravelling the complex interactions between genetics, animal age and nutrition as they impact on tissue deposition, muscle characteristics and quality of Australian sheep meat. Australian Journal of Experimental Agriculture 47, 1229–1238.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Wilson GR, Edwards MJ (2008) Native wildlife on rangelands to minimise methane and produce lower-emission meat: Kangaroos versus livestock. Conservation Letters 1, 119–128.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Zhong WWH, Lucas CA, Hoh JFY (2008) Myosin isoforms and fibre types in limb muscles of Australian marsupials: Adaptations to hopping and non-hopping locomotion. Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology 178, 47–55.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1