What can we deduce about the reproductive condition of spinifex hopping mice (Notomys alexis) from external examination?
Karleah K. Berris A C , William G. Breed A and Susan M. Carthew B
+ Author Affiliations
- Author Affiliations
A School of Biological Sciences, The University of Adelaide, Adelaide, SA 5005, Australia.
B Research Institute for Environment and Livelihoods, Charles Darwin University, Casuarina, NT 0909, Australia.
C Corresponding author. Email: karleah.trengove@gmail.com
Australian Mammalogy 42(1) 11-15 https://doi.org/10.1071/AM18038
Submitted: 27 September 2018 Accepted: 07 January 2019 Published: 7 February 2019
Abstract
Field studies often use external examination of the vagina or the swelling of the scrotum to make deductions about the reproductive condition of rodents. In this study we sought to determine what information on reproductive condition could be gained from external examination of the reproductive anatomy of spinifex hopping mice (Notomys alexis) using individuals from a captive colony. Female N. alexis with perforate vaginae had a significantly larger mean uterine mass and larger mean maximum ovarian follicle diameter than non-perforate females. Corpora lutea were recorded in two perforate animals but were not present in females with a ‘pinhole’-size perforate or non-perforate vagina. In male hopping mice scrotal bulge size was unrelated to testes mass, ventral prostate mass or presence/absence of sperm in the cauda epididymides. Males with dark scrotal pigmentation had a significantly smaller mean testes mass than males with light or no pigmentation. However, there was no relationship between scrotal pigmentation and ventral prostate mass or sperm presence in the cauda epididymides. Our study suggests that vaginal perforation is an acceptable indicator of sexual maturity in female hopping mice, but scrotal bulge size and scrotal pigmentation should not be used to predict reproductive condition of males.
Additional keywords: anatomy, Muridae, reproduction.
References
Allen, E. (1922). The oestrous cycle in the mouse. The American Journal of Anatomy 30, 297–371.| The oestrous cycle in the mouse.Crossref | GoogleScholarGoogle Scholar |
Atlas of Living Australia (2018). Notomys alexis Thomas, 1922, spinifex hopping-mouse. Available at: https://bie.ala.org.au/species/urn:lsid:biodiversity.org.au:afd.taxon:49001532-929e-4b78-97d3-c885e97d671b [accessed 15 July 2018].
Bauer, M., and Breed, W. G. (2006). Variation of sperm head shape and tail length in a species of Australian hydromyine rodent: the spinifex hopping mouse, Notomys alexis. Reproduction, Fertility and Development 18, 797–805.
| Variation of sperm head shape and tail length in a species of Australian hydromyine rodent: the spinifex hopping mouse, Notomys alexis.Crossref | GoogleScholarGoogle Scholar |
Bauer, M., and Breed, W. G. (2008). Testis mass of the spinifex hopping mouse and its impact on fertility potential. Journal of Zoology 274, 349–356.
| Testis mass of the spinifex hopping mouse and its impact on fertility potential.Crossref | GoogleScholarGoogle Scholar |
Breed, W. G. (1975). Environmental factors and reproduction in the female hopping mouse, Notomys alexis. Journal of Reproduction and Fertility 45, 273–281.
| Environmental factors and reproduction in the female hopping mouse, Notomys alexis.Crossref | GoogleScholarGoogle Scholar | 1239506PubMed |
Breed, W. G. (1981). Histology of accessory sex organs and extragonadal sperm reserves in the male hopping mouse Notomys alexis. Archives of Andrology 7, 357–360.
| Histology of accessory sex organs and extragonadal sperm reserves in the male hopping mouse Notomys alexis.Crossref | GoogleScholarGoogle Scholar | 7316609PubMed |
Breed, W. G. (1982). Morphological variation in the testes and accessory sex organs of Australian rodents in the genera Pseudomys and Notomys. Journal of Reproduction and Fertility 66, 607–613.
| Morphological variation in the testes and accessory sex organs of Australian rodents in the genera Pseudomys and Notomys.Crossref | GoogleScholarGoogle Scholar | 7175815PubMed |
Breed, W. G. (1989). Comparative studies on the reproductive biology of three species of laboratory bred Australian conilurine rodents (Muridae: Hydromyinae). Journal of Zoology 217, 683–699.
| Comparative studies on the reproductive biology of three species of laboratory bred Australian conilurine rodents (Muridae: Hydromyinae).Crossref | GoogleScholarGoogle Scholar |
Breed, W. G. (1995). Spinifex hopping-mouse. In ‘The Mammals of Australia’. (Ed. R. Strahan.) pp. 568–570. (Reed Books: Sydney.)
Breed, W. G., and Leigh, C. M. (2011). Reproductive biology of an Old Endemic murid rodent of Australia, the spinifex hopping mouse, Notomys alexis: adaptations for life in the arid zone. Integrative Zoology 6, 321–333.
| Reproductive biology of an Old Endemic murid rodent of Australia, the spinifex hopping mouse, Notomys alexis: adaptations for life in the arid zone.Crossref | GoogleScholarGoogle Scholar | 22182324PubMed |
Breed, W. G., and Taylor, J. (2000). Body mass, testes mass, and sperm size in murine rodents. Journal of Mammalogy 81, 758–768.
| Body mass, testes mass, and sperm size in murine rodents.Crossref | GoogleScholarGoogle Scholar |
Breed, W. G., Leigh, C. M., and Speight, N. (2013). Coevolution of the male and female reproductive tracts in an Old Endemic murine rodent of Australia. Journal of Zoology 289, 94–100.
| Coevolution of the male and female reproductive tracts in an Old Endemic murine rodent of Australia.Crossref | GoogleScholarGoogle Scholar |
Breed, W. G., Leigh, C. M., and Breed, M. F. (2017). Changes in abundance and reproductive activity of small arid-zone murid rodents on an active cattle station in central Australia. Wildlife Research 44, 22–27.
| Changes in abundance and reproductive activity of small arid-zone murid rodents on an active cattle station in central Australia.Crossref | GoogleScholarGoogle Scholar |
Campbell, I. (2007). Chi-squared and Fisher–Irwin tests of two-by-two tables with small sample recommendations. Statistics in Medicine 26, 3661–3675.
| Chi-squared and Fisher–Irwin tests of two-by-two tables with small sample recommendations.Crossref | GoogleScholarGoogle Scholar | 17315184PubMed |
Dickman, C. R., Predavec, M., and Downey, F. J. (1995). Long-range movements of small mammals in arid Australia: implications for land management. Journal of Arid Environments 31, 441–452.
| Long-range movements of small mammals in arid Australia: implications for land management.Crossref | GoogleScholarGoogle Scholar |
Dickman, C. R., Mahon, P. S., Masters, P., and Gibson, D. F. (1999). Long-term dynamics of rodent populations in arid Australia: the influence of rainfall. Wildlife Research 26, 389–403.
| Long-term dynamics of rodent populations in arid Australia: the influence of rainfall.Crossref | GoogleScholarGoogle Scholar |
Dickman, C. R., Greenville, A. C., Beh, C. L., Tamayo, B., and Wardle, G. M. (2010). Social organisation and movements of desert rodents during population “booms” and “busts” in central Australia. Journal of Mammalogy 91, 798–810.
| Social organisation and movements of desert rodents during population “booms” and “busts” in central Australia.Crossref | GoogleScholarGoogle Scholar |
Fox, B. J., and Kemper, C. M. (1982). Growth and development of Pseudomys gracilicaudatus (Rodentia: Muridae) in the laboratory. Australian Journal of Zoology 30, 175–185.
| Growth and development of Pseudomys gracilicaudatus (Rodentia: Muridae) in the laboratory.Crossref | GoogleScholarGoogle Scholar |
Giraudoux, P., Antonietti, J. P., Beale, C., Pleydell, D., and Treglia, M. (2018). Package ‘pgirmess’: spatial analysis and data mining for field ecologists (R package version 1.6.9). Available at: http://perso.orange.fr/giraudoux [accessed 13 December 2018].
Kenagy, G. J., and Trombulak, S. C. (1986). Size and function of mammalian testes in relation to body size. Journal of Mammalogy 67, 1–22.
| Size and function of mammalian testes in relation to body size.Crossref | GoogleScholarGoogle Scholar |
Masters, P. (1993). The effects of fire-driven succession and rainfall on small mammals in spinifex grassland at Uluru National Park, Northern Territory. Wildlife Research 20, 803–813.
| The effects of fire-driven succession and rainfall on small mammals in spinifex grassland at Uluru National Park, Northern Territory.Crossref | GoogleScholarGoogle Scholar |
Peirce, E. J., and Breed, W. G. (1989). Light microscopical structure of the excurrent ducts and distribution of spermatozoa in the Australian rodents Pseudomys australis and Notomys alexis. Journal of Anatomy 162, 195–213.
| 2808117PubMed |
Peirce, E. J., Moore, H. D. M., Leigh, C. M., and Breed, W. G. (2003). Studies on sperm storage in the vas deferens of the spinifex hopping mouse (Notomys alexis). Reproduction (Cambridge, England) 125, 233–240.
| Studies on sperm storage in the vas deferens of the spinifex hopping mouse (Notomys alexis).Crossref | GoogleScholarGoogle Scholar |
Predavec, M. (1994). Population dynamics and environmental changes during natural irruptions of Australian desert rodents. Wildlife Research 21, 569–582.
| Population dynamics and environmental changes during natural irruptions of Australian desert rodents.Crossref | GoogleScholarGoogle Scholar |
Pye, T. (1991). The New Holland mouse (Pseudomys novaehollandiae) (Rodentia: Muridae) in Tasmania: a field study. Wildlife Research 18, 521–531.
| The New Holland mouse (Pseudomys novaehollandiae) (Rodentia: Muridae) in Tasmania: a field study.Crossref | GoogleScholarGoogle Scholar |
R Core Team (2018). R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. Available at: http://www.R-project.org/ [accessed 15 May 2018].
Siegel, J. S., and Castellan, J. N. (1988). ‘Nonparametric Statistics for the Behavioural Sciences.’ (McGraw-Hill: New York.)
Southgate, R., and Masters, P. (1996). Fluctuations of rodent populations in response to rainfall and fire in a central Australian hummock grassland dominated by Plectrachne schinzii. Wildlife Research 23, 289–303.
| Fluctuations of rodent populations in response to rainfall and fire in a central Australian hummock grassland dominated by Plectrachne schinzii.Crossref | GoogleScholarGoogle Scholar |
Suttle, J. M., Moore, H. D. M., Peirce, E. J., and Breed, W. G. (1988). Quantitative studies on variation in sperm head morphology of the hopping mouse, Notomys alexis. The Journal of Experimental Zoology 247, 166–171.
| Quantitative studies on variation in sperm head morphology of the hopping mouse, Notomys alexis.Crossref | GoogleScholarGoogle Scholar |
Woods, R. E., and Ford, F. D. (2000). Observations on the behaviour of the smoky mouse Pseudomys fumeus (Rodentia: Muridae). Australian Mammalogy 22, 35–42.
| Observations on the behaviour of the smoky mouse Pseudomys fumeus (Rodentia: Muridae).Crossref | GoogleScholarGoogle Scholar |
